Use of ADP/ATP transporter genes to increase nitrogen use efficiency and low nitrogen tolerance to a plant

ABSTRACT

Provided are methods of increasing nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to SEQ ID NO: 2560, 2557, 184, 238, 188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2562 or 2563. Also provided are isolated polynucleotides and polypeptides which can be used to increase nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content and/or abiotic stress tolerance of a plant of a plant.

RELATED APPLICATIONS

This application is a division of U.S. patent application Ser. No.13/059,231 filed Feb. 16, 2011, which is a National Phase of PCT PatentApplication No. PCT/IB2009/053633 having International filing date ofAug. 18, 2009, which claims the benefit of priority of U.S. ProvisionalPatent Application No. 61/136,189 filed on Aug. 18, 2008. The contentsof the above applications are all incorporated by reference as if fullyset forth herein in their entirety.

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 61683SequenceListing.txt, created on Feb. 16,2015, comprising 5,350,431 bytes, submitted concurrently with the filingof this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolypeptides and polynucleotides, nucleic acid constructs comprisingsame, transgenic plants expressing same and methods of using same forincreasing nitrogen use efficiency, yield, biomass, vigor, growth rate,oil content, fertilizer use efficiency, water use efficiency and abioticstress tolerance of a plant.

A common approach to promote plant growth has been, and continues to be,the use of natural as well as synthetic nutrients (fertilizers). Thus,fertilizers are the fuel behind the “green revolution”, directlyresponsible for the exceptional increase in crop yields during the last40 years, and are considered the number one overhead expense inagriculture. Of the three macronutrients provided as main fertilizers[Nitrogen (N), Phosphate (P) and Potassium (K)], nitrogen is the onlyone that usually needs to be replenished every year, particularly forcereals, which comprise more than half of the cultivated areasworldwide. For example, inorganic nitrogenous fertilizers such asammonium nitrate, potassium nitrate, or urea, typically accounts for 40%of the costs associated with crops such as corn and wheat.

Nitrogen is an essential macronutrient for the plant, responsible forbiosynthesis of amino and nucleic acids, prosthetic groups, planthormones, plant chemical defenses, etc. In addition, nitrogen is oftenthe rate-limiting element in plant growth and all field crops have afundamental dependence on inorganic nitrogen. Thus, nitrogen is totranslocated to the shoot, where it is stored in the leaves and stalkduring the rapid step of plant development and up until flowering. Incorn for example, plants accumulate the bulk of their organic nitrogenduring the period of grain germination, and until flowering. Oncefertilization of the plant has occurred, grains begin to form and becomethe main sink of plant nitrogen. The stored nitrogen can be thenredistributed from the leaves and stalk that served as storagecompartments until grain formation.

Since fertilizer is rapidly depleted from most soil types, it must besupplied to growing crops two or three times during the growing season.In addition, the low nitrogen use efficiency (NUE) of the main crops(e.g., in the range of only 30-70%) negatively affects the inputexpenses for the farmer, due to the excess fertilizer applied. Moreover,the over and inefficient use of fertilizers are major factorsresponsible for environmental problems such as eutrophication ofgroundwater, lakes, rivers and seas, nitrate pollution in drinking waterwhich can cause methemoglobinemia, phosphate pollution, atmosphericpollution and the like. However, in spite of the negative impact offertilizers on the environment, and the limits on fertilizer use, whichhave been legislated in several countries, the use of fertilizers isexpected to increase in order support food and fiber production forrapid population growth on limited land resources. For example, it hasbeen estimated that by 2050, more than 150 million tons of nitrogenousfertilizer will be used worldwide annually.

Increased use efficiency of nitrogen by plants should enable crops to becultivated with lower fertilizer input, or alternatively to becultivated on soils of poorer quality and would therefore havesignificant economic impact in both developed and developingagricultural systems.

Genetic improvement of fertilizer use efficiency (FUE) in plants can begenerated either via traditional breeding or via genetic engineering.

-   Attempts to generate plants with increased FUE have been described    in U.S. Pat. Appl. No. 20020046419 to Choo, et al.; U.S. Pat. Appl.    No. 2005010879 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511    to Chomet et al.; Good, A, et al. 2007 (Engineering nitrogen use    efficiency with alanine aminotransferase. Canadian Journal of Botany    85: 252-262); and Good A G et al. 2004 (Trends Plant Sci.    9:597-605).

Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S.A. 2004 101:7833-8)describe Dof1 transgenic plants which exhibit improved growth underlow-nitrogen conditions.

U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stressresponsive promoter to control the expression of Alanine AmineTransferase (AlaAT) and transgenic canola plants with improved droughtand nitrogen deficiency tolerance when compared to control plants.

The global shortage of water supply, desertification, abiotic stress(ABS) conditions (e.g., drought, salinity, osmoticum, flood, suboptimaltemperatures such as cold and heat, toxic chemical pollution, radiation,nutrient deficiencies, and the like) together with the presence oflimited nitrogen and fertilizer sources cause substantial damage toagricultural plants such as major alterations in the plant metabolism,cell death, and decreases in plant growth and crop productivity.

Drought is a gradual phenomenon, which involves periods of abnormallydry weather that persists long enough to produce serious hydrologicimbalances such as crop damage, water supply shortage and increasedsusceptibility to various diseases.

Salinity affects one in five hectares of irrigated land. None of the topfive food crops, i.e., wheat, corn, rice, potatoes, and soybean, cantolerate excessive salt. Detrimental effects of salt on plants resultfrom both water deficit, which leads to osmotic stress (similar todrought stress), and the effect of excess sodium ions on criticalbiochemical processes. As with freezing and drought, high salt causeswater deficit; and the presence of high salt makes it difficult forplant roots to extract water from their environment. Thus, salination ofsoils that are used for agricultural production is a significant andincreasing problem in regions that rely heavily on agriculture, and isworsen by over-utilization, over-fertilization and water shortage,typically caused by climatic change and the demands of increasingpopulation.

Extreme temperatures such as suboptimal or heat temperatures affectplant growth and development through the whole plant life cycle. Thus,low temperatures reduce germination rate and high temperatures result inleaf necrosis. In addition, mature plants that are exposed to excess ofheat may experience heat shock, which may arise in various organs,including leaves and particularly fruit, when transpiration isinsufficient to overcome heat stress. Heat also damages cellularstructures, including organelles and cytoskeleton, and impairs membranefunction. Heat shock may produce a decrease in overall proteinsynthesis, accompanied by expression of heat shock proteins, e.g.,chaperones, which are involved in refolding proteins denatured by heat.High-temperature damage to pollen almost always occurs in conjunctionwith drought stress, and rarely occurs under well-watered conditions.Combined stress can alter plant metabolism in novel ways. Excessivechilling conditions, e.g., low, but above freezing, temperatures affectcrops of tropical origins, such as soybean, rice, maize, and cotton.Typical chilling damage includes wilting, necrosis, chlorosis or leakageof ions from cell membranes. Excessive light conditions, which occurunder clear atmospheric conditions subsequent to cold late summer/autumnnights, can lead to photoinhibition of photosynthesis (disruption ofphotosynthesis). In addition, chilling may lead to yield losses andlower product quality through the delayed ripening of maize.

Nutrient deficiencies cause adaptations of the root architecture,particularly notably for example is the root proliferation withinnutrient rich patches to increase nutrient uptake. Nutrient deficienciescause also the activation of plant metabolic pathways which maximize theabsorption, assimilation and distribution processes such as byactivating architectural changes. Engineering the expression of thetriggered genes may cause the plant to exhibit the architectural changesand enhanced metabolism also under other conditions.

In addition, it is widely known that the plants usually respond to waterdeficiency by creating a deeper root system that allows access tomoisture located in deeper soil layers. Triggering this effect willallow the plants to access nutrients and water located in deeper soilhorizons particularly those readily dissolved in water like nitrates.

Yield is affected by various factors, such as, the number and size ofthe plant organs, plant architecture (for example, the number ofbranches), grains set length, number of filled grains, vigor (e.g.seedling), growth rate, root development, utilization of water,nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.

Crops such as, corn, rice, wheat, canola and soybean account for overhalf of total human caloric intake, whether through direct consumptionof the seeds themselves or through consumption of meat products raisedon processed seeds or forage. Seeds are also a source of sugars,proteins and oils and metabolites used in industrial processes. Theability to increase plant yield, whether through increase dry matteraccumulation rate, modifying cellulose or lignin composition, increasestalk strength, enlarge meristem size, change of plant branchingpattern, erectness of leaves, increase in fertilization efficiency,enhanced seed dry matter accumulation rate, modification of seeddevelopment, enhanced seed filling or by increasing the content of oil,starch or protein in the seeds would have many applications inagricultural and non-agricultural uses such as in the biotechnologicalproduction of pharmaceuticals, antibodies or vaccines.

WO publication No. 2009/013750 discloses genes, constructs and methodsof increasing abiotic stress tolerance, biomass and/or yield in plantsgenerated thereby.

WO publication No. 2008/122980 discloses genes constructs and methodsfor increasing oil content, growth rate and biomass of plants.

WO publication No. 2008/075364 discloses polynucleotides involved inplant fiber development and methods of using same.

WO publication No. 2007/049275 discloses isolated polypeptides,polynucleotides encoding same, transgenic plants expressing same andmethods of using same for increasing fertilizer use efficiency, plantabiotic stress tolerance and biomass.

WO publication No. 2004/104162 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2005/121364 discloses polynucleotides andpolypeptides involved in plant fiber development and methods of usingsame for improving fiber quality, yield and/or biomass of a fiberproducing plant.

WO publication No. 2007/020638 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence at least 80% identical to SEQ ID NO: 2506, 2512,2442, 2496, 2446, 1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128,130-137, 270-287, 289-293, 295-306, 308-362, 364-666, 671, 673-1333,2414-2441, 2443-2445, 2447-2455, 2458-2495, 2497-2505, 2507-2511,2513-2521 or 2522, thereby increasing the nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising thenucleic acid sequence selected from the group consisting of SEQ ID NOs:2506, 2512, 2442, 2496, 2446, 1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60,63-128, 130-137, 270-287, 289-293, 295-306, 308-362, 364-666, 671,673-1333, 2414-2441, 2443-2445, 2447-2455, 2458-2495, 2497-2505,2507-2511, 2513-2522, 3, 5, 6, 9, 10, 14, 15, 20, 61, 62, 129, 288, 294,307, 363, 667, 668, 669, 670, 672, 2398-2413, 2456 and 2457, therebyincreasing the nitrogen use efficiency, fertilizer use efficiency,yield, growth rate, vigor, biomass, oil content and/or abiotic stresstolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least 80% identical toSEQ ID NO: 2557, 2560, 184, 238, 188, 154-156, 158-161, 163-183,185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457,1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559,2561-2562 or 2563, thereby increasing the nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide selected from the groupconsisting of SEQ ID NOs: 2557, 2560, 184, 238, 188, 154-156, 158-161,163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425,1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558,2559, 2561-2563, 138-143, 146, 148, 150-152, 157, 162, 198, 265,1334-1350, 1352-1364, 1426-1428, 1458, 1460, 1732-1734, 1737-1738,2523-2532, 2542 and 2543, thereby increasing the nitrogen useefficiency, fertilizer use efficiency, yield, growth rate, vigor,biomass, oil content and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency and/or oil content of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence at least 80% identical to SEQ ID NO: 3, 5, 6, 9,10, 14, 15, 288, 294, 2398-2412 or 2413, thereby increasing the nitrogenuse efficiency, fertilizer use efficiency, and/or oil content of theplant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency and/or oil content of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising thenucleic acid sequence selected from the group consisting of SEQ ID NOs:3, 5, 6, 9, 10, 14, 15, 288, 294, 2398-2413, thereby increasing thenitrogen use efficiency, fertilizer use efficiency and/or oil content ofthe plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency and/or oil content of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least 80% identical toSEQ ID NO: 138-153, 1334-1350, 1352-1364, 1458, 1460, 2523-2531 or 2532,thereby increasing the nitrogen use efficiency, fertilizer useefficiency and/or oil content of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency,fertilizer use efficiency and/or oil content of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide selected from the groupconsisting of SEQ ID NOs: 138-153, 1334-1350, 1352-1364, 1458, 1460,2523-2532, thereby increasing the nitrogen use efficiency, fertilizeruse efficiency and/or oil content of the plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence at least 80% identical to SEQ ID NO: 2506, 2512, 2442, 2496,2446, 1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2521 or 2522, whereinthe nucleic acid sequence is capable of increasing nitrogen useefficiency, fertilizer use efficiency, yield, growth rate, vigor,biomass, oil content and/or abiotic stress of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 2506, 2512,2442, 2496, 2446, 1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128,130-137, 270-287, 289-293, 295-306, 308-362, 364-666, 671, 673-1333,2414-2441, 2443-2445, 2447-2455, 2458-2495, 2497-2505, 2507-2511,2513-2522, 3, 5, 6, 9, 10, 14, 15, 20, 61, 62, 129, 288, 294, 307, 363,667, 668, 669, 670, 672, 2398-2413, 2456 and 2457.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises an amino acid sequenceat least 80% homologous to the amino acid sequence set forth in SEQ IDNO: 2557, 2560, 184, 238, 188, 154-156, 158-161, 163-183, 185-187,189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459,1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2562or 2563, wherein the amino acid sequence is capable of increasingnitrogen use efficiency, fertilizer use efficiency, yield, growth rate,vigor, biomass, oil content and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises the amino acid sequenceselected from the group consisting of SEQ ID NOs: 2557, 2560, 184, 238,188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237, 239-264,266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397,2533-2541, 2544-2556, 2558, 2559, 2561-2563, 138-143, 146, 148, 150-152,157, 162, 198, 265, 1334-1350, 1352-1364, 1426-1428, 1458, 1460,1732-1734, 1737-1738, 2523-2532, 2542 and 2543.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising the isolatedpolynucleotide of the invention, and a promoter for directingtranscription of the nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising an amino acidsequence at least 80% homologous to SEQ ID NO: 2557, 2560, 184, 238,188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237, 239-264,266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397,2533-2541, 2544-2556, 2558, 2559, 2561-2562 or 2563, wherein the aminoacid sequence is capable of increasing nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising the amino acidsequence selected from the group consisting of SEQ ID NOs: 2557, 2560,184, 238, 188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237,239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735,1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2563, 138-143, 146,148, 150-152, 157, 162, 198, 265, 1334-1350, 1352-1364, 1426-1428, 1458,1460, 1732-1734, 1737-1738, 2523-2532, 2542 and 2543.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polynucleotideof the invention, or the nucleic acid construct of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polypeptide ofthe invention.

According to some embodiments of the invention, the nucleic acidsequence is as set forth in SEQ ID NO: 2506, 2512, 2442, 2496, 2446, 1,2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287, 289-293,295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2521 or 2522.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs: 2506, 2512, 2442, 2496, 2446, 1, 2, 4, 7, 8, 11, 12, 13,16-19, 21-60, 63-128, 130-137, 270-287, 289-293, 295-306, 308-362,364-666, 671, 673-1333, 2414-2441, 2443-2445, 2447-2455, 2458-2495,2497-2505, 2507-2511, 2513-2522.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence at least 80% homologous to SEQID NO: 2557, 2560, 184, 238, 188, 154-156, 158-161, 163-183, 185-187,189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459,1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2562or 2563.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 2557, 2560, 184, 238, 188, 154-156, 158-161,163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425,1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558,2559, 2561-2563.

According to some embodiments of the invention, the nucleic acidsequence is as set forth in SEQ ID NO: 3, 5, 6, 9, 10, 14, 15, 288, 294,2398-2412 or 2413.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs: 3, 5, 6, 9, 10, 14, 15, 288, 294, 2398-2413.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence at least 80% homologous to SEQID NO: 138-153, 1334-1350, 1352-1364, 1458, 1460, 2523-2531 or 2532.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 138-153, 1334-1350, 1352-1364, 1458, 1460,2523-2532.

According to some embodiments of the invention, the plant cell formspart of a plant.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

According to some embodiments of the invention, the abiotic stress isselected from the group consisting of salinity, drought, waterdeprivation, flood, etiolation, low temperature, high temperature, heavymetal toxicity, anaerobiosis, nutrient deficiency, nutrient excess,atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the yield comprises seedyield or oil yield.

Unless otherwise defined, all technical and/or scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which the invention pertains. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of embodiments of the invention, exemplarymethods and/or materials are described below. In case of conflict, thepatent specification, including definitions, will control. In addition,the materials, methods, and examples are illustrative only and are notintended to be necessarily limiting.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

Some embodiments of the invention are herein described, by way ofexample only, with reference to the accompanying drawings. With specificreference now to the drawings in detail, it is stressed that theparticulars shown are by way of example and for purposes of illustrativediscussion of embodiments of the invention. In this regard, thedescription taken with the drawings makes apparent to those skilled inthe art how embodiments of the invention may be practiced.

In the Drawings:

FIG. 1 is a schematic illustration of the pGI binary plasmid used forexpressing the isolated polynucleotide sequences of some embodiments ofthe invention. RB—T-DNA right border; LB—T-DNA left border; H—HindIIIrestriction enzyme; X—XbaI restriction enzyme; B—BamHI restrictionenzyme; S—SalI restriction enzyme; Sm—SmaI restriction enzyme; R-I—EcoRIrestriction enzyme; Sc—SacI/SstI/Ecl136II; (numbers)—Length inbase-pairs; NOS pro=nopaline synthase promoter; NPT-II=neomycinphosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-Asignal (polyadenylation signal); GUSintron—the GUS reporter gene (codingsequence and intron) The isolated polynucleotide sequences of theinvention were cloned into the vector while replacing the GUSintronreporter gene

FIG. 2 is a schematic illustration of the modified pGI binary plasmidused for expressing the isolated polynucleotide sequences of theinvention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiplecloning site; RE—any restriction enzyme; (numbers)—Length in base-pairs;NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferasegene; NOS ter=nopaline synthase terminator; Poly-A signal(polyadenylation signal); GUSintron—the GUS reporter gene (codingsequence and intron) The isolated polynucleotide sequences of theinvention were cloned into the vector while replacing the GUSintronreporter gene.

FIGS. 3A-B are images depicting visualization of root development ofplants grown in transparent agar plates. The different transgenes weregrown in transparent agar plates for 10 days and the plates werephotographed every 3-4 days starting at day 1. FIG. 3A—An image of aphotograph of plants taken following 10 days on agar plates. FIG. 3B—Animage of root analysis in which the length of the root measured isrepresented by a red arrow.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolynucleotides and polypeptides, expression vectors comprising same andtransgenic plants expressing same and, more particularly, but notexclusively, to methods of increasing nitrogen use efficiency, yield,biomass, vigor, growth rate, oil content and abiotic stress tolerance ofa plant using same.

Before explaining at least one embodiment of the invention in detail, itis to be understood that the invention is not necessarily limited in itsapplication to the details set forth in the following description orexemplified by the Examples. The invention is capable of otherembodiments or of being practiced or carried out in various ways.

While reducing the present invention to practice, the present inventorshave identified novel polypeptides and polynucleotides which can be usedto increase nitrogen use efficiency, fertilizer use efficiency, wateruse efficiency, yield, growth rate, biomass, oil content, vigor and/orabiotic stress tolerance of a plant.

Thus, as shown in the Examples section which follows, the presentinventors have utilized bioinformatics tools to generate digitalexpression profiles of gene clusters which expression level isassociated with various conditions and stresses such as nutrientdeficiency, cold, salinity, drought, heat stress, etiolation,waterlogging and oxidative stress (Tables 1-19; Example 1 of theExamples section which follows), and based on the expression profileshave identified genes which are expected to enhance nitrogen useefficiency, biomass, growth rate, yield, vigor, oil content and/orabiotic stress tolerance of a plant (Table 20; polynucleotide SEQ IDNOs:1-137; polypeptides SEQ ID NOs:138-269; Example 1 of the Examplessection which follows). Homologous polypeptides and polynucleotideshaving the same function were also identified (Table 21, polynucleotideSEQ ID NOs:270-1333; polypeptide SEQ ID NOs:1334-2397; Example 2 of theExamples section which follows). To test the effect of the isolatedgenes on the trait-of-interest, the polynucleotides were cloned intobinary vectors (Table 23, polynucleotide SEQ ID NOs: 2398-2522; Example3 of the Examples section which follows) and the predicted proteins wereidentified (Table 23, Example 3). Transgenic plants over-expressing theidentified polynucleotides were found to exhibit increased nitrogen useefficiency, yield, biomass, photosynthetic areas and growth rate (Tables24-521 Examples 5, 6 and 7 of the Examples section which follows), aswell as increased abiotic stress tolerance (e.g., under salinity stress;Tables 53-55, Example 8 of the Examples section which follows).Altogether, these results suggest the use of the novel polynucleotidesand polypeptides of the invention and homologous sequences thereof forincreasing nitrogen use efficiency, fertilizer use efficiency, yield(including oil yield, seed yield and oil content), growth rate, biomass,vigor and/or abiotic stress tolerance of a plant.

Thus, according to an aspect of some embodiments of the invention, thereis provided method of increasing fertilizer use efficiency, nitrogen useefficiency, yield, growth rate, vigor, biomass, oil content and/orabiotic stress tolerance of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequence atleast 80% identical to SEQ ID NO: 2506, 2512, 2442, 2496, 2446, 1, 2, 4,7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287, 289-293,295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2521 or 2522, therebyincreasing the nitrogen use efficiency, fertilizer use efficiency,yield, growth rate, vigor, biomass, oil content, water use efficiencyand/or abiotic stress tolerance of the plant.

As used herein the phrase “fertilizer use efficiency” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per fertilizer unit applied. Themetabolic process can be the uptake, spread, absorbent, accumulation,relocation (within the plant) and use of one or more of the minerals andorganic moieties absorbed by the plant, such as nitrogen, phosphatesand/or potassium.

As used herein the phrase “fertilizer-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) of afertilizer applied which is below the level needed for normal plantmetabolism, growth, reproduction and/or viability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per nitrogen unit applied. The metabolicprocess can be the uptake, spread, absorbent, accumulation, relocation(within the plant) and use of nitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) ofnitrogen (e.g., ammonium or nitrate) applied which is below the levelneeded for normal plant metabolism, growth, reproduction and/orviability.

Improved plant NUE and FUE is translated in the field into eitherharvesting similar quantities of yield, while implementing lessfertilizers, or increased yields gained by implementing the same levelsof fertilizers. Thus, improved NUE or FUE has a direct effect on plantyield in the field. Thus, the polynucleotides and polypeptides of someembodiments of the invention positively affect plant yield, seed yield,and plant biomass. In addition, the benefit of improved plant NUE willcertainly improve crop quality and biochemical constituents of the seedsuch as protein yield and oil yield.

As used herein the phrase “plant yield” refers to the amount (asdetermined by weight or size) or quantity (numbers) of tissue producedper plant or per growing season. Hence increased yield could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time.

It should be noted that a plant yield can be affected by variousparameters including, but not limited to, plant biomass; plant vigor;growth rate; seed yield; seed or grain quantity; seed or grain quality;oil yield; content of oil, starch and/or protein in harvested organs(e.g., seeds or vegetative parts of the plant); number of flowers(florets) per panicle (expressed as a ratio of number of filled seedsover number of primary panicles); harvest index; number of plants grownper area; number and size of harvested organs per plant and per area;number of plants per growing area (density); number of harvested organsin field; total leaf area; carbon assimilation and carbon partitioning(the distribution/allocation of carbon within the plant); resistance toshade; number of harvestable organs (e.g. seeds), seeds per pod, weightper seed; and modified architecture [such as increase stalk diameter,thickness or improvement of physical properties (e.g. elasticity)].

As used herein the phrase “plant biomass” refers to the amount (measuredin grams of air-dry tissue) of a tissue produced from the plant in agrowing season, which could also determine or affect the plant yield orthe yield per growing area. An increase in plant biomass can be in thewhole plant or in parts thereof such as aboveground (harvestable) parts,vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plantorgan size or mass per time (can be measured in cm² per day, day or asthe regression coefficient of along time course).

As used herein the phrase “plant vigor” refers to the amount (measuredby weight) of tissue produced by the plant in a given time. Henceincreased vigor could determine or affect the plant yield or the yieldper growing time or growing area. In addition, early vigor (seed and/orseedling) result with improved field stand.

As used herein the phrase “seed yield” refers to the number or weight ofthe seeds per plant, seeds per pod, or per growing area or to the weightof a single seed, or to the oil extracted per seed. Hence seed yield canbe affected by seed dimensions (e.g., length, width, perimeter, areaand/or volume), number of (filled) seeds and seed filling rate and byseed oil content. Hence increase seed yield per plant could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time; and increase seed yield per growing area could beachieved by increasing seed yield per plant, and/or by increasing numberof plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used hereinrefers to a small embryonic plant enclosed in a covering called the seedcoat (usually with some stored food), the product of the ripened ovuleof gymnosperm and angiosperm plants which occurs after fertilization andsome growth within the mother plant.

The phrase “oil content” as used herein refers to the amount of lipidsin a given plant organ, either the seeds (seed oil content) or thevegetative portion of the plant (vegetative oil content) and istypically expressed as percentage of dry weight (10% humidity of seeds)or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oilproduction of a tissue (e.g., seed, vegetative portion), as well as themass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achievedby increasing the size/mass of a plant's tissue(s) which comprise oilper growth period. Thus, increased oil content of a plant can beachieved by increasing the yield, growth rate, biomass and vigor of theplant.

It should be noted that a plant yield can be determined under stress(e.g., abiotic stress, nitrogen-limiting conditions) or non-stress(normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growthconditions (e.g., water, temperature, light-dark cycles, humidity, saltconcentration, fertilizer concentration in soil, nutrient supply such asnitrogen, phosphorous and/or potassium), which enable normal metabolism,growth, reproduction and/or viability of a plant at any stage in itslife cycle (from seed to mature plant and back to seed again). It shouldbe noted that while the non-stress conditions may include some mildvariations from the optimal conditions (which vary from one type/speciesof a plant to another), such variations do not cause the plant to ceasegrowing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effecton metabolism, growth, reproduction and/or viability of a plant.Accordingly, abiotic stress can be induced by suboptimal environmentalgrowth conditions such as, for example, salinity, water deprivation,flooding, freezing, low or high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, atmospheric pollution or UVirradiation. The implications of abiotic stress are discussed in theBackground section.

The phrase “abiotic stress tolerance” as used herein refers to theability of a plant to endure an abiotic stress without suffering asubstantial alteration in metabolism, growth, productivity and/orviability.

As used herein the phrase “water use efficiency (WUE)” refers to thelevel of organic matter produced per unit of water consumed by theplant, i.e., the dry weight of a plant in relation to the plant's wateruse, e.g., the biomass produced per unit transpiration.

As used herein the term “increasing” refers to at least about 2%, atleast about 3%, at least about 4%, at least about 5%, at least about10%, at least about 15%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, increase in nitrogen use efficiency, fertilizeruse efficiency, yield, growth rate, vigor, biomass, oil content, wateruse efficiency and/or abiotic stress tolerance of a plant as compared toa native plant [i.e., a plant not modified with the biomolecules(polynucleotide or polypeptides) of the invention, e.g., anon-transformed plant of the same species which is grown under the samegrowth conditions).

As used herein, the phrase “exogenous polynucleotide” refers to aheterologous nucleic acid sequence which may not be naturally expressedwithin the plant or which overexpression in the plant is desired. Theexogenous polynucleotide may be introduced into the plant in a stable ortransient manner, so as to produce a ribonucleic acid (RNA) moleculeand/or a polypeptide molecule. It should be noted that the exogenouspolynucleotide may comprise a nucleic acid sequence which is identicalor partially homologous to an endogenous nucleic acid sequence of theplant.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 2506, 2512, 2442, 2496, 2446, 1, 2, 4, 7, 8,11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287, 289-293, 295-306,308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445, 2447-2455,2458-2495, 2497-2505, 2507-2511, 2513-2522.

Identity (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastN software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs: 2506, 2512, 2442, 2496, 2446,1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2522.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO: 2506, 2512, 2442, 2496, 2446,1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2521 or 2522.

As used herein the term “polynucleotide” refers to a single or doublestranded nucleic acid sequence which is isolated and provided in theform of an RNA sequence, a complementary polynucleotide sequence (cDNA),a genomic polynucleotide sequence and/or a composite polynucleotidesequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from thenatural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refersto a sequence, which results from reverse transcription of messenger RNAusing a reverse transcriptase or any other RNA dependent DNA polymerase.Such a sequence can be subsequently amplified in vivo or in vitro usinga DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to asequence derived (isolated) from a chromosome and thus it represents acontiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers toa sequence, which is at least partially complementary and at leastpartially genomic. A composite sequence can include some exonalsequences required to encode the polypeptide of the present invention,as well as some intronic sequences interposing therebetween. Theintronic sequences can be of any source, including of other genes, andtypically will include conserved splicing signal sequences. Suchintronic sequences may further include cis acting expression regulatoryelements.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% homologous to the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 2557, 2560, 184, 238, 188, 154-156, 158-161,163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425,1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558,2559, 2561-2563.

Homology (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastP or TBLASTNsoftware of the National Center of Biotechnology Information (NCBI) suchas by using default parameters, when starting from a polypeptidesequence; or the tBLASTX algorithm (available via the NCBI) such as byusing default parameters, which compares the six-frame conceptualtranslation products of a nucleotide query sequence (both strands)against a protein sequence database.

Homologous sequences include both orthologous and paralogous sequences.The term “paralogous” relates to gene-duplications within the genome ofa species leading to paralogous genes. The term “orthologous” relates tohomologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is byperforming a reciprocal blast search. This may be done by a first blastinvolving blasting the sequence-of-interest against any sequencedatabase, such as the publicly available NCBI database which may befound at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov. If orthologues in rice were sought, thesequence-of-interest would be blasted against, for example, the 28,469full-length cDNA clones from Oryza sativa Nipponbare available at NCBI.The blast results may be filtered. The full-length sequences of eitherthe filtered results or the non-filtered results are then blasted back(second blast) against the sequences of the organism from which thesequence-of-interest is derived. The results of the first and secondblasts are then compared. An orthologue is identified when the sequenceresulting in the highest score (best hit) in the first blast identifiesin the second blast the query sequence (the originalsequence-of-interest) as the best hit. Using the same rational aparalogue (homolog to a gene in the same organism) is found. In case oflarge sequence families, the ClustalW program may be used [HypertextTransfer Protocol://World Wide Web (dot) ebi (dot) ac (dot)uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joiningtree (Hypertext Transfer Protocol://en (dot) wikipedia (dot)org/wiki/Neighbor-joining) which helps visualizing the clustering.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 2557, 2560, 184, 238, 188, 154-156,158-161, 163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351,1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541,2544-2556, 2558, 2559, 2561-2562 or 2563.

According to an aspect of some embodiments of the invention there isprovided a method of increasing nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content and/orabiotic stress tolerance of a plant. The method is effected byexpressing within the plant an exogenous polynucleotide. comprising thenucleic acid sequence selected from the group consisting of SEQ ID NOs:2506, 2512, 2442, 2496, 2446, 1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60,63-128, 130-137, 270-287, 289-293, 295-306, 308-362, 364-666, 671,673-1333, 2414-2441, 2443-2445, 2447-2455, 2458-2495, 2497-2505,2507-2511, 2513-2522, 3, 5, 6, 9, 10, 14, 15, 20, 61, 62, 129, 288, 294,307, 363, 667, 668, 669, 670, 672, 2398-2413, 2456 and 2457, therebyincreasing the nitrogen use efficiency, fertilizer use efficiency,yield, growth rate, vigor, biomass, oil content and/or abiotic stresstolerance of the plant.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO: 2506, 2512, 2442, 2496, 2446,1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2522, 3, 5, 6, 9, 10,14, 15, 20, 61, 62, 129, 288, 294, 307, 363, 667, 668, 669, 670, 672,2398-2413, 2456 or 2457.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence selected from the group consisting of SEQ ID NOs: 2557,2560, 184, 238, 188, 154-156, 158-161, 163-183, 185-187, 189-197,200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730,1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2563, 138-143,146, 148, 150-152, 157, 162, 198, 265, 1334-1350, 1352-1364, 1426-1428,1458, 1460, 1732-1734, 1737-1738, 2523-2532, 2542 and 2543, therebyincreasing the nitrogen use efficiency, fertilizer use efficiency,yield, growth rate, vigor, biomass, oil content and/or abiotic stresstolerance of the plant.

According to an aspect of some embodiments of the invention there isprovided a method of increasing nitrogen use efficiency, fertilizer useefficiency and/or oil content of a plant. The method is effected byexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence at least 80% identical to SEQ ID NO: 3, 5, 6, 9,10, 14, 15, 288, 294, 2398-2412 or 2413, thereby increasing the nitrogenuse efficiency, fertilizer use efficiency, and/or oil content of theplant.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 3, 5, 6, 9, 10, 14, 15, 288, 294, 2398-2413.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs: 3, 5, 6, 9, 10, 14, 15, 288,294, 2398-2413.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO: 3, 5, 6, 9, 10, 14, 15, 288,294, 2398-2412 or 2413.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% homologous to the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 138-153, 1334-1350, 1352-1364, 1458, 1460,2523-2532.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 138-153, 1334-1350, 1352-1364, 1458,1460, 2523-2531 or 2532.

Nucleic acid sequences encoding the polypeptides of the presentinvention may be optimized for expression. Non-limiting examples ofoptimized nucleic acid sequences are provided in SEQ ID NOs: 2415, 2420,2428, 2430, 2431, 2436, 2437, 2441, 2444, 2445, 2446, 2451, 2456, 2468,2471, 2478, 2481, 2484, 2520 and 2522 (Table 23). Examples of suchsequence modifications include, but are not limited to, an altered G/Ccontent to more closely approach that typically found in the plantspecies of interest, and the removal of codons atypically found in theplant species commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriateDNA nucleotides for use within a structural gene or fragment thereofthat approaches codon usage within the plant of interest. Therefore, anoptimized gene or nucleic acid sequence refers to a gene in which thenucleotide sequence of a native or naturally occurring gene has beenmodified in order to utilize statistically-preferred orstatistically-favored codons within the plant. The nucleotide sequencetypically is examined at the DNA level and the coding region optimizedfor expression in the plant species determined using any suitableprocedure, for example as described in Sardana et al. (1996, Plant CellReports 15:677-681). In this method, the standard deviation of codonusage, a measure of codon usage bias, may be calculated by first findingthe squared proportional deviation of usage of each codon of the nativegene relative to that of highly expressed plant genes, followed by acalculation of the average squared deviation. The formula used is: 1SDCU=n=1 N[(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage ofcodon n in highly expressed plant genes, where Yn to the frequency ofusage of codon n in the gene of interest and N refers to the totalnumber of codons in the gene of interest. A Table of codon usage fromhighly expressed genes of dicotyledonous plants is compiled using thedata of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance withthe preferred codon usage for a particular plant cell type is based onthe direct use, without performing any extra statistical calculations,of codon optimization Tables such as those provided on-line at the CodonUsage Database through the NIAS (National Institute of AgrobiologicalSciences) DNA bank in Japan (Hypertext Transfer Protocol://World WideWeb (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Databasecontains codon usage tables for a number of different species, with eachcodon usage Table having been statistically determined based on the datapresent in Genbank.

By using the above Tables to determine the most preferred or mostfavored codons for each amino acid in a particular species (for example,rice), a naturally-occurring nucleotide sequence encoding a protein ofinterest can be codon optimized for that particular plant species. Thisis effected by replacing codons that may have a low statisticalincidence in the particular species genome with corresponding codons, inregard to an amino acid, that are statistically more favored. However,one or more less-favored codons may be selected to delete existingrestriction sites, to create new ones at potentially useful junctions(5′ and 3′ ends to add signal peptide or termination cassettes, internalsites that might be used to cut and splice segments together to producea correct full-length sequence), or to eliminate nucleotide sequencesthat may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, inadvance of any modification, contain a number of codons that correspondto a statistically-favored codon in a particular plant species.Therefore, codon optimization of the native nucleotide sequence maycomprise determining which codons, within the native nucleotidesequence, are not statistically-favored with regards to a particularplant, and modifying these codons in accordance with a codon usage tableof the particular plant to produce a codon optimized derivative. Amodified nucleotide sequence may be fully or partially optimized forplant codon usage provided that the protein encoded by the modifiednucleotide sequence is produced at a level higher than the proteinencoded by the corresponding naturally occurring or native gene.Construction of synthetic genes by altering the codon usage is describedin for example PCT Patent Application 93/07278.

According to some embodiments of the invention, the exogenouspolynucleotide is a non-coding RNA.

As used herein the phrase ‘non-coding RNA” refers to an RNA moleculewhich does not encode an amino acid sequence (a polypeptide). Examplesof such non-coding RNA molecules include, but are not limited to, anantisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor ofa Piwi-interacting RNA (piRNA).

According to a specific embodiment the non-coding polynucleotidecomprises the nucleic acid sequence of SEQ ID NO: 64 or 2459 (NUE512).

Thus, the invention encompasses nucleic acid sequences describedhereinabove; fragments thereof, sequences hybridizable therewith,sequences homologous thereto, sequences encoding similar polypeptideswith different codon usage, altered sequences characterized bymutations, such as deletion, insertion or substitution of one or morenucleotides, either naturally occurring or man induced, either randomlyor in a targeted fashion.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs: 2506, 2512, 2442, 2496, 2446,1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2522.

According to some embodiments of the invention the nucleic acid sequenceis capable of increasing nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, abioticstress tolerance and/or water use efficiency of a plant.

According to some embodiments of the invention the isolatedpolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs: 2506, 2512, 2442, 2496, 2446, 1, 2, 4,7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287, 289-293,295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2522, 3, 5, 6, 9, 10,14, 15, 20, 61, 62, 129, 288, 294, 307, 363, 667, 668, 669, 670, 672,2398-2413, 2456 and 2457.

According to some embodiments of the invention the isolatedpolynucleotide is set forth by SEQ ID NO: 2506, 2512, 2442, 2496, 2446,1, 2, 4, 7, 8, 11, 12, 13, 16-19, 21-60, 63-128, 130-137, 270-287,289-293, 295-306, 308-362, 364-666, 671, 673-1333, 2414-2441, 2443-2445,2447-2455, 2458-2495, 2497-2505, 2507-2511, 2513-2522, 3, 5, 6, 9, 10,14, 15, 20, 61, 62, 129, 288, 294, 307, 363, 667, 668, 669, 670, 672,2398-2413, 2456 or 2457.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NOs: 2557, 2560, 184, 238, 188,154-156, 158-161, 163-183, 185-187, 189-197, 200-237, 239-264, 266-269,1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541,2544-2556, 2558, 2559, 2561-2563.

According to some embodiments of the invention the amino acid sequenceis capable of increasing nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, abioticstress tolerance and/or water use efficiency of a plant.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises the amino acidsequence selected from the group consisting of SEQ ID NOs: 2557, 2560,184, 238, 188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237,239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735,1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2563, 138-143, 146,148, 150-152, 157, 162, 198, 265, 1334-1350, 1352-1364, 1426-1428, 1458,1460, 1732-1734, 1737-1738, 2523-2532, 2542 and 2543.

The invention provides an isolated polypeptide comprising an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to an amino acid sequence selected fromthe group consisting of SEQ ID NOs: 2557, 2560, 184, 238, 188, 154-156,158-161, 163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351,1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541,2544-2556, 2558, 2559, 2561-2563.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID NOs: 2557, 2560, 184, 238, 188, 154-156, 158-161, 163-183,185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457,1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559,2561-2563, 138-143, 146, 148, 150-152, 157, 162, 198, 265, 1334-1350,1352-1364, 1426-1428, 1458, 1460, 1732-1734, 1737-1738, 2523-2532, 2542and 2543.

According to some embodiments of the invention, the polypeptide is setforth by SEQ ID NO: 2557, 2560, 184, 238, 188, 154-156, 158-161,163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425,1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558,2559, 2561-2563, 138-143, 146, 148, 150-152, 157, 162, 198, 265,1334-1350, 1352-1364, 1426-1428, 1458, 1460, 1732-1734, 1737-1738,2523-2532, 2542 or 2543.

The invention also encompasses fragments of the above describedpolypeptides and polypeptides having mutations, such as deletions,insertions or substitutions of one or more amino acids, either naturallyoccurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses whole plants, ancestors andprogeny of the plants and plant parts, including seeds, shoots, stems,roots (including tubers), and plant cells, tissues and organs. The plantmay be in any form including suspension cultures, embryos, meristematicregions, callus tissue, leaves, gametophytes, sporophytes, pollen, andmicrospores. Plants that are particularly useful in the methods of theinvention include all plants which belong to the superfamilyViridiplantae, in particular monocotyledonous and dicotyledonous plantsincluding a fodder or forage legume, ornamental plant, food crop, tree,or shrub selected from the list comprising Acacia spp., Acer spp.,Actinidia spp., Aesculus spp., Agathis australis, Albizia amara,Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Asteliafragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassicaspp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadabafarinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicumspp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomumcassia, Coffea arabica, Colophospermum mopane, Coronillia varia,Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp.,Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogonspp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davalliadivaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogonamplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloapyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp.,Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa,Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp,Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycinejavanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtiacoleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus,Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffheliadissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia,Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex,Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihotesculenta, Medicago saliva, Metasequoia glyptostroboides, Musasapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryzaspp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petuniaspp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photiniaspp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara,Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopiscineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis,Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhusnatalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosaspp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitysvefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghumbicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides,Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themedatriandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vacciniumspp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschiaaethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brusselssprouts, cabbage, canola, carrot, cauliflower, celery, collard greens,flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean,straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize,wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton,rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, atree, an ornamental plant, a perennial grass and a forage crop.Alternatively algae and other non-Viridiplantae can be used for themethods of the present invention.

According to some embodiments of the invention, the plant used by themethod of the invention is a crop plant such as rice, maize, wheat,barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean,sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea),flax, lupinus, rapeseed, tobacco, poplar and cotton.

According to some embodiments of the invention, there is provided aplant cell exogenously expressing the polynucleotide of some embodimentsof the invention, the nucleic acid construct of some embodiments of theinvention and/or the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, expressing the exogenouspolynucleotide of the invention within the plant is effected bytransforming one or more cells of the plant with the exogenouspolynucleotide, followed by generating a mature plant from thetransformed cells and cultivating the mature plant under conditionssuitable for expressing the exogenous polynucleotide within the matureplant.

According to some embodiments of the invention, the transformation iseffected by introducing to the plant cell a nucleic acid construct whichincludes the exogenous polynucleotide of some embodiments of theinvention and at least one promoter for directing transcription of theexogenous polynucleotide in a host cell (a plant cell). Further detailsof suitable transformation approaches are provided hereinbelow.

According to some embodiments of the invention, there is provided anucleic acid construct comprising the isolated polynucleotide of theinvention, and a promoter for directing transcription of the nucleicacid sequence of the isolated polynucleotide in a host cell.

According to some embodiments of the invention, the isolatedpolynucleotide is operably linked to the promoter sequence.

A coding nucleic acid sequence is “operably linked” to a regulatorysequence (e.g., promoter) if the regulatory sequence is capable ofexerting a regulatory effect on the coding sequence linked thereto.

As used herein, the term “promoter” refers to a region of DNA which liesupstream of the transcriptional initiation site of a gene to which RNApolymerase binds to initiate transcription of RNA. The promoter controlswhere (e.g., which portion of a plant) and/or when (e.g., at which stageor condition in the lifetime of an organism) the gene is expressed.

Any suitable promoter sequence can be used by the nucleic acid constructof the present invention. Preferably the promoter is a constitutivepromoter, a tissue-specific, or an abiotic stress-inducible promoter.

Suitable constitutive promoters include, for example, CaMV 35S promoter(SEQ ID NO: 3063; Odell et al., Nature 313:810-812, 1985); ArabidopsisAt6669 promoter (SEQ ID NO:3064; see PCT Publication No. WO04081173A2);maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992);rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last etal., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al.,Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant JNovember; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant Mol.Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant MolBiol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen.Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1); 107-121,1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76,1995). Other constitutive promoters include those in U.S. Pat. Nos.5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785;5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to,leaf-specific promoters [such as described, for example, by Yamamoto etal., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67,1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor etal., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol.23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specificgenes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al.,J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol.14: 633, 1990), Brazil Nut albumin (Pearson' et al., Plant Mol. Biol.18: 235-245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214,1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22,1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et alPlant Mol Biol, 143). 323-32 1990), napA (Stalberg, et al, Planta 199:515-519, 1996), Wheat SPA (Albani et al, Plant Cell, 9: 171-184, 1997),sunflower oleosin (Cummins, etal., Plant Mol. Biol. 19: 873-876, 1992)],endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (MolGen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins(EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein(Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1):53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosaet al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulinGlb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), ricealpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22,1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR genefamily (Plant J 12:235-46, 1997), sorgum gamma-kafirin (PMB 32:1029-35,1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc.Nati. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma of al, PlantMol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem.,123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalenesynthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109,1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245; 1989),apetala-3].

Suitable abiotic stress-inducible promoters include, but not limited to,salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al.,Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such asmaize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266,1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295,1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol.39:373-380, 1999); heat-inducible promoters such as heat tomatohsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention canfurther include an appropriate selectable marker and/or an origin ofreplication. According to some embodiments of the invention, the nucleicacid construct utilized is a shuttle vector, which can propagate both inE. coli (wherein the construct comprises an appropriate selectablemarker and origin of replication) and be compatible with propagation incells. The construct according to the present invention can be, forexample, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus oran artificial chromosome.

The nucleic acid construct of some embodiments of the invention can beutilized to stably or transiently transform plant cells. In stabletransformation, the exogenous polynucleotide is integrated into theplant genome and as such it represents a stable and inherited trait. Intransient transformation, the exogenous polynucleotide is expressed bythe cell transformed but it is not integrated into the genome and assuch it represents a transient trait.

There are various methods of introducing foreign genes into bothmonocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev.Plant. Physiol, Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al,Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNAinto plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev.Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes, eds. Schell, J., and Vasil, L. K., Academic Publishers, SanDiego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds.Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass.(1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego,Calif. (1989) p. 52-68; including methods for direct uptake of DNA intoprotoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNAuptake induced by brief electric shock of plant cells: Zhang et al.Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986)319:791-793. DNA injection into plant cells or tissues by particlebombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al.Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990)79:206-209; by the use of micropipette systems: Neuhaus et al., Theor.Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant.(1990) 79:213-217; glass fibers or silicon carbide whiskertransformation of cell cultures, embryos or callus tissue, U.S. Pat. No.5,464,765 or by the direct incubation of DNA with germinating pollen,DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman,G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p.197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors thatcontain defined DNA segments that integrate into the plant genomic DNA.Methods of inoculation of the plant tissue vary depending upon the plantspecies and the Agrobacterium delivery system. A widely used approach isthe leaf disc procedure which can be performed with any tissue explantthat provides a good source for initiation of whole plantdifferentiation. See, e.g., Horsch et al. in Plant Molecular BiologyManual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. Asupplementary approach employs the Agrobacterium delivery system incombination with vacuum infiltration. The Agrobacterium system isespecially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. Inelectroporation, the protoplasts are briefly exposed to a strongelectric field. In microinjection, the DNA is mechanically injecteddirectly into the cells using very small micropipettes. In microparticlebombardment, the DNA is adsorbed on microprojectiles such as magnesiumsulfate crystals or tungsten particles, and the microprojectiles arephysically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The mostcommon method of plant propagation is by seed. Regeneration by seedpropagation, however, has the deficiency that due to heterozygositythere is a lack of uniformity in the crop, since seeds are produced byplants according to the genetic variances governed by Mendelian rules.Basically, each seed is genetically different and each will grow withits own specific traits. Therefore, it is preferred that the transformedplant be produced such that the regenerated plant has the identicaltraits and characteristics of the parent transgenic plant. Therefore, itis preferred that the transformed plant be regenerated bymicropropagation which provides a rapid, consistent reproduction of thetransformed plants.

Micropropagation is a process of growing new generation plants from asingle piece of tissue that has been excised from a selected parentplant or cultivar. This process permits the mass reproduction of plantshaving the preferred tissue expressing the fusion protein. The newgeneration plants which are produced are genetically identical to, andhave all of the characteristics of, the original plant. Micropropagationallows mass production of quality plant material in a short period oftime and offers a rapid multiplication of selected cultivars in thepreservation of the characteristics of the original transgenic ortransformed plant. The advantages of cloning plants are the speed ofplant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration ofculture medium or growth conditions between stages. Thus, themicropropagation process involves four basic stages: Stage one, initialtissue culturing; stage two, tissue culture multiplication; stage three,differentiation and plant formation; and stage four, greenhouseculturing and hardening. During stage one, initial tissue culturing, thetissue culture is established and certified contaminant-free. Duringstage two, the initial tissue culture is multiplied until a sufficientnumber of tissue samples are produced to meet production goals. Duringstage three, the tissue samples grown in stage two are divided and growninto individual plantlets. At stage four, the transformed plantlets aretransferred to a greenhouse for hardening where the plants' tolerance tolight is gradually increased so that it can be grown in the naturalenvironment.

According to some embodiments of the invention, the transgenic plantsare generated by transient transformation of leaf cells, meristematiccells or the whole plant.

Transient transformation can be effected by any of the direct DNAtransfer methods described above or by viral infection using modifiedplant viruses.

Viruses that have been shown to be useful for the transformation ofplant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus(BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation ofplants using plant viruses is described in U.S. Pat. No. 4,855,237 (beangolden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese PublishedApplication No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); andGluzman, Y. et al., Communications in Molecular Biology: Viral Vectors,Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirusparticles for use in expressing foreign DNA in many hosts, includingplants are described in WO 87/06261.

According to some embodiments of the invention, the virus used fortransient transformations is avirulent and thus is incapable of causingsevere symptoms such as reduced growth rate, mosaic, ring spots, leafroll, yellowing, streaking, pox formation, tumor formation and pitting.A suitable avirulent virus may be a naturally occurring avirulent virusor an artificially attenuated virus. Virus attenuation may be effectedby using methods well known in the art including, but not limited to,sub-lethal heating, chemical treatment or by directed mutagenesistechniques such as described, for example, by Kurihara and Watanabe(Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992),Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as,for example, the American Type culture Collection (ATCC) or by isolationfrom infected plants. Isolation of viruses from infected plant tissuescan be effected by techniques well known in the art such as described,for example by Foster and Tatlor, Eds. “Plant Virology Protocols: FromVirus Isolation to Transgenic Resistance (Methods in Molecular Biology(Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of aninfected plant believed to contain a high concentration of a suitablevirus, preferably young leaves and flower petals, are ground in a buffersolution (e.g., phosphate buffer solution) to produce a virus infectedsap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression ofnon-viral exogenous polynucleotide sequences in plants is demonstratedby the above references as well as by Dawson, W. O. et al., Virology(1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French etal. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990)269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to thevirus itself. Alternatively, the virus can first be cloned into abacterial plasmid for ease of constructing the desired viral vector withthe foreign DNA. The virus can then be excised from the plasmid. If thevirus is a DNA virus, a bacterial origin of replication can be attachedto the viral DNA, which is then replicated by the bacteria.Transcription and translation of this DNA will produce the coat proteinwhich will encapsidate the viral DNA. If the virus is an RNA virus, thevirus is generally cloned as a cDNA and inserted into a plasmid. Theplasmid is then used to make all of the constructions. The RNA virus isthen produced by transcribing the viral sequence of the plasmid andtranslation of the viral genes to produce the coat protein(s) whichencapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which thenative coat protein coding sequence has been deleted from a viralpolynucleotide, a non-native plant viral coat protein coding sequenceand a non-native promoter, preferably the subgenomic promoter of thenon-native coat protein coding sequence, capable of expression in theplant host, packaging of the recombinant plant viral polynucleotide, andensuring a systemic infection of the host by the recombinant plant viralpolynucleotide, has been inserted. Alternatively, the coat protein genemay be inactivated by insertion of the non-native polynucleotidesequence within it, such that a protein is produced. The recombinantplant viral polynucleotide may contain one or more additional non-nativesubgenomic promoters. Each non-native subgenomic promoter is capable oftranscribing or expressing adjacent genes or polynucleotide sequences inthe plant host and incapable of recombination with each other and withnative subgenomic promoters. Non-native (foreign) polynucleotidesequences may be inserted adjacent the native plant viral subgenomicpromoter or the native and a non-native plant viral subgenomic promotersif more than one polynucleotide sequence is included. The non-nativepolynucleotide sequences are transcribed or expressed in the host plantunder control of the subgenomic promoter to produce the desiredproducts.

In a second embodiment, a recombinant plant viral polynucleotide isprovided as in the first embodiment except that the native coat proteincoding sequence is placed adjacent one of the non-native coat proteinsubgenomic promoters instead of a non-native coat protein codingsequence.

In a third embodiment, a recombinant plant viral polynucleotide isprovided in which the native coat protein gene is adjacent itssubgenomic promoter and one or more non-native subgenomic promoters havebeen inserted into the viral polynucleotide. The inserted non-nativesubgenomic promoters are capable of transcribing or expressing adjacentgenes in a plant host and are incapable of recombination with each otherand with native subgenomic promoters. Non-native polynucleotidesequences may be inserted adjacent the non-native subgenomic plant viralpromoters such that the sequences are transcribed or expressed in thehost plant under control of the subgenomic promoters to produce thedesired product.

In a fourth embodiment, a recombinant plant viral polynucleotide isprovided as in the third embodiment except that the native coat proteincoding sequence is replaced by a non-native coat protein codingsequence.

The viral vectors are encapsidated by the coat proteins encoded by therecombinant plant viral polynucleotide to produce a recombinant plantvirus. The recombinant plant viral polynucleotide or recombinant plantvirus is used to infect appropriate host plants. The recombinant plantviral polynucleotide is capable of replication in the host, systemicspread in the host, and transcription or expression of foreign gene(s)(exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Fosterand Taylor, eds. “Plant Virology Protocols: From Virus Isolation toTransgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods inVirology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A.“Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A.“Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa,eds. “Principles and Techniques in Plant Virology”, VanNostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present inventioncan also be introduced into a chloroplast genome thereby enablingchloroplast expression.

A technique for introducing exogenous polynucleotide sequences to thegenome of the chloroplasts is known. This technique involves thefollowing procedures. First, plant cells are chemically treated so as toreduce the number of chloroplasts per cell to about one. Then, theexogenous polynucleotide is introduced via particle bombardment into thecells with the aim of introducing at least one exogenous polynucleotidemolecule into the chloroplasts. The exogenous polynucleotides selectedsuch that it is integratable into the chloroplast's genome viahomologous recombination which is readily effected by enzymes inherentto the chloroplast. To this end, the exogenous polynucleotide includes,in addition to a gene of interest, at least one polynucleotide stretchwhich is derived from the chloroplast's genome. In addition, theexogenous polynucleotide includes a selectable marker, which serves bysequential selection procedures to ascertain that all or substantiallyall of the copies of the chloroplast genomes following such selectionwill include the exogenous polynucleotide. Further details relating tothis technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507which are incorporated herein by reference. A polypeptide can thus beproduced by the protein expression system of the chloroplast and becomeintegrated into the chloroplast's inner membrane.

Since processes which increase oil content, yield, growth rate, biomass,vigor and/or abiotic stress tolerance of a plant can involve multiplegenes acting additively or in synergy (see, for example, in Quesda etal., Plant Physiol. 130:951-063, 2002), the present invention alsoenvisages expressing a plurality of exogenous polynucleotides in asingle host plant to thereby achieve superior effect on oil content,yield, growth rate, biomass, vigor and/or abiotic stress tolerance.

Expressing a plurality of exogenous polynucleotides in a single hostplant can be effected by co-introducing multiple nucleic acidconstructs, each including a different exogenous polynucleotide, into asingle plant cell. The transformed cell can than be regenerated into amature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by co-introducing into a singleplant-cell a single nucleic-acid construct including a plurality ofdifferent exogenous polynucleotides. Such a construct can be designedwith a single promoter sequence which can transcribe a polycistronicmessenger RNA including all the different exogenous polynucleotidesequences. To enable co-translation of the different polypeptidesencoded by the polycistronic messenger RNA, the polynucleotide sequencescan be inter-linked via an internal ribosome entry site (IRES) sequencewhich facilitates translation of polynucleotide sequences positioneddownstream of the IRES sequence. In this case, a transcribedpolycistronic RNA molecule encoding the different polypeptides describedabove will be translated from both the capped 5′ end and the twointernal IRES sequences of the polycistronic RNA molecule to therebyproduce in the cell all different polypeptides. Alternatively, theconstruct can include several promoter sequences each linked to adifferent exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality ofdifferent exogenous polynucleotides, can be regenerated into a matureplant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by introducing different nucleic acidconstructs, including different exogenous polynucleotides, into aplurality of plants. The regenerated transformed plants can then becross-bred and resultant progeny selected for superior abiotic stresstolerance, water use efficiency, fertilizer use efficiency, growth,biomass, yield and/or vigor traits, using conventional plant breedingtechniques.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

Non-limiting examples of abiotic stress conditions include, salinity,drought, water deprivation, excess of water (e.g., flood, waterlogging),etiolation, low temperature, high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, nutrient excess, atmosphericpollution and UV irradiation.

Thus, the invention encompasses plants exogenously expressing thepolynucleotide(s), the nucleic acid constructs and/or polypeptide(s) ofthe invention. Once expressed within the plant cell or the entire plant,the level of the polypeptide encoded by the exogenous polynucleotide canbe determined by methods well known in the art such as, activity assays,Western blots using antibodies capable of specifically binding thepolypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA),radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry,immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribedfrom the exogenous polynucleotide are well known in the art and include,for example, Northern blot analysis, reverse transcription polymerasechain reaction (RT-PCR) analysis (including quantitative,semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

In addition, the endogenous homolog of the exogenous polynucleotide orpolypeptide of the invention, or a fragment of the endogenous homolog(e.g. introns or untranslated regions) in the plant can be used as amarker for marker assisted selection (MAS), in which a marker is usedfor indirect selection of a genetic determinant or determinants of atrait of interest (e.g., biomass, growth rate, oil content, yield,abiotic stress tolerance). These genes (DNA or RNA sequence) may containor be linked to polymorphic sites or genetic markers on the genome suchas restriction fragment length polymorphism (RFLP), microsatellites andsingle nucleotide polymorphism (SNP), DNA fingerprinting (DFP),amplified fragment length polymorphism (AFLP), expression levelpolymorphism, polymorphism of the encoded polypeptide and any otherpolymorphism at the DNA or RNA sequence.

Examples of marker assisted selections include, but are not limited to,selection for a morphological trait (e.g., a gene that affects form,coloration, male sterility or resistance such as the presence or absenceof awn, leaf sheath coloration, height, grain color, aroma of rice);selection for a biochemical trait (e.g., a gene that encodes a proteinthat can be extracted and observed; for example, isozymes and storageproteins); selection for a biological trait (e.g., pathogen races orinsect biotypes based on host pathogen or host parasite interaction canbe used as a marker since the genetic constitution of an organism canaffect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be usedin a wide range of economical plants, in a safe and cost effectivemanner.

Plant lines exogenously expressing the polynucleotide or the polypeptideof the invention are screened to identify those that show the greatestincrease of the desired plant trait.

Following is a non-limiting description of assays which can be used todetermine the effect of the transgene (the exogenous polynucleotide ofsome embodiments of the invention) or is encoded polypeptide on thetrait-of-interest in a plant.

The main parameters of efficiency used to define plant Nitrogen (N)metabolism include nitrogen-uptake efficiency, nitrogen utilizationefficiency, and nitrogen-use efficiency

The Nitrogen-uptake efficiency [the amount of N in above ground biomass(grams of nitrogen)/N applied (grams/hectare)] is the total amount ofnitrogen incorporated by the plant and is a function of the “uptake”(the plant's transport capacity), the metabolic efficiency of theassimilation process and the rate of plant size development, since themass of stalk and leaves created during growth are the actualNitrogen-storage organs. The fraction of the assimilated Nitrogen foundin a shoot that is ultimately transferred to the grain (yield) iscontrolled enzymatically, and thus can be affected by transgenicmanipulation. This parameter is, in effect, equal to the Nitrogen Useefficiency (NUE). Better grain-to-shoot N-partitioning most likely willimprove yield and protein content of the grain.

Similarly, the same calculations of use and utilization efficiencies canbe made for other macronutrients such as Phosphorous (P) and Potassium(K), which have a direct correlation with yield and general planttolerance.

Fertilizer Use Efficiency—

To analyze whether the transgenic plants are more responsive tofertilizers, plants are grown in agar plates or pots with a limitedamount of fertilizer, as described, for example, in Examples 5-7 of theExample section which follows and in Yanagisawa et al (Proc Natl AcadSci USA. 2004; 101:7833-8). The plants are analyzed for their overallsize, time to flowering, yield, protein content of shoot and/or grain.The parameters checked are the overall size of the mature plant, its wetand dry weight, the weight of the seeds yielded, the average seed sizeand the number of seeds produced per plant. Other parameters that may betested are: the chlorophyll content of leaves (as nitrogen plant statusand the degree of leaf verdure is highly correlated), amino acid and thetotal protein content of the seeds or other plant parts such as leavesor shoots, oil content, etc. Similarly, instead of providing nitrogen atlimiting amounts, phosphate or potassium can be added at increasingconcentrations. Again, the same parameters measured are the same aslisted above. In this way, nitrogen use efficiency (NUE), phosphate useefficiency (PUE) and potassium use efficiency (KUE) are assessed,checking the ability of the transgenic plants to thrive under nutrientrestraining conditions.

Nitrogen Use Efficiency—

To analyze whether the transgenic Arabidopsis plants are more responsiveto nitrogen, plant are grown in 0.75-1.5 mM (nitrogen deficientconditions) or 6-15 mM (optimal nitrogen concentration). Plants areallowed to grow for additional 20-40 days or until seed production. Theplants are then analyzed for their overall size, time to flowering,yield, protein content of shoot and/or grain/seed production. Theparameters checked can be the overall size of the plant, wet and dryweight, the weight of the seeds yielded, the average seed size and thenumber of seeds produced per plant. Other parameters that may be testedare: the chlorophyll content of leaves (as nitrogen plant status and thedegree of leaf greenness is highly correlated), amino acid and the totalprotein content of the seeds or other plant parts such as leaves orshoots and oil content. Transformed plants not exhibiting substantialphysiological and/or morphological effects, or exhibiting highermeasured parameters levels than wild-type plants, are identified asnitrogen use efficient plants.

Nitrogen Determination—

The procedure for N (nitrogen) concentration determination in thestructural parts of the plants involves the potassium persulfatedigestion method to convert organic N to NO₃ ⁻ (Purcell and King 1996Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃ ⁻ toNO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) and the measurement ofnitrite by the Griess assay (Vodovotz 1996, supra). The absorbancevalues are measured at 550 nm against a standard curve of NaNO₂. Theprocedure is described in details in Samonte et al. 2006 Agron. J.98:168-176.

Germination Tests—

Germination tests compare the percentage of seeds from transgenic plantsthat could complete the germination process to the percentage of seedsfrom control plants that are treated in the same manner. Normalconditions are considered for example, incubations at 22° C. under22-hour light 2-hour dark daily cycles. Evaluation of germination andseedling vigor is conducted between 4 and 14 days after planting. Thebasal media is 50% MS medium (Murashige and Skoog, 1962 Plant Physiology15, 473-497).

Germination is checked also at unfavorable conditions such as cold(incubating at temperatures lower than 10° C. instead of 22° C.) orusing seed inhibition solutions that contain high concentrations of anosmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM,300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrationsof salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene on plant's vigor, growth rate, biomass,yield and/or oil content can be determined using known methods.

Plant Vigor—

The plant vigor can be calculated by the increase in growth parameterssuch as leaf area, fiber length, rosette diameter, plant fresh weightand the like per time.

Growth Rate—

The growth rate can be measured using digital analysis of growingplants. For example, images of plants growing in greenhouse on plotbasis can be captured every 3 days and the rosette area can becalculated by digital analysis. Rosette area growth is calculated usingthe difference of rosette area between days of sampling divided by thedifference in days between samples.

Evaluation of growth rate can be done by measuring plant biomassproduced, rosette area, leaf size or root length per time (can bemeasured in cm² per day of leaf area).

Relative growth area can be calculated using Formula I.Relative growth area rate=(ΔArea/Δt)*(1/Areat ₀)  Formula I:

Δt is the current analyzed image day subtracted from the initial day(t−t0). Thus, the relative growth area rate is in units of 1/day andlength growth rate is in units of 1/day.

Alternatively, the relative growth rate of the area can be calculated asthe regression coefficient along time course.

Seed Yield—

Evaluation of the seed yield per plant can be done by measuring theamount (weight or size) or quantity (i.e., number) of dry seeds producedand harvested from 8-16 plants and divided by the number of plants.

For example, the total seeds from 8-16 plants can be collected, weightedusing e.g., an analytical balance and the total weight can be divided bythe number of plants. Seed yield per growing area can be calculated inthe same manner while taking into account the growing area given to asingle plant. Increase seed yield per growing area could be achieved byincreasing seed yield per plant, and/or by increasing number of plantscapable of growing in a given area.

In addition, seed yield can be determined via the weight of 1000 seeds.The weight of 1000 seeds can be determined as follows: seeds arescattered on a glass tray and a picture is taken. Each sample isweighted and then using the digital analysis, the number of seeds ineach sample is calculated.

The 1000 seeds weight can be calculated using formula II:1000 Seed Weight=number of seed in sample/sample weight×1000  FormulaII:

The Harvest Index can be calculated using Formula IIIHarvest Index=Average seed yield per plant/Average dry weight  FormulaIII:

Grain Protein Concentration—

Grain protein content (grams grain protein m⁻²) is estimated as theproduct of the mass of grain N (nitrogen) [grams grain Nitrogen m⁻²]multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990,supra). The grain protein concentration is estimated as the ratio ofgrain protein content per unit mass of the grain (grams grain proteinkg⁻¹ grain).

Fiber Length—

Fiber length can be measured using fibrograph. The fibrograph system wasused to compute length in terms of “Upper Half Mean” length. The upperhalf mean (UHM) is the average length of longer half of the fiberdistribution. The fibrograph measures length in span lengths at a givenpercentage point (Hypertext Transfer Protocol://World Wide Web (dot)cottonine (dot) com/ClassificationofCotton/?Pg=4#Length).

Oil Content—

The oil content of a plant can be determined by extraction of the oilfrom the seed or the vegetative portion of the plant. Briefly, lipids(oil) can be removed from the plant (e.g., seed) by grinding the planttissue in the presence of specific solvents (e.g., hexane or petroleumether) and extracting the oil in a continuous extractor. Indirect oilcontent analysis can be carried out using various known methods such asNuclear Magnetic Resonance (NMR) Spectroscopy, which measures theresonance energy absorbed by hydrogen atoms in the liquid state of thesample [See for example, Conway T F. and Earle F R., 1963, Journal ofthe American Oil Chemists' Society; Springer Berlin/Heidelberg, ISSN:0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI)Spectroscopy, which utilizes the absorption of near infrared energy(1100-2500 nm) by the sample; and a method described in WO/2001/023884,which is based on extracting oil a solvent, evaporating the solvent in agas stream which forms oil particles, and directing a light into the gasstream and oil particles which forms a detectable reflected light.

The effect of the transgene or its encoded polypeptide on abiotic stresstolerance can be determined using known methods.

Abiotic Stress Tolerance—

Transformed (i.e., expressing the transgene) and non-transformed (wildtype) plants are exposed to an abiotic stress condition, such as waterdeprivation, suboptimal temperature (low temperature, high temperature),nutrient deficiency, nutrient excess, a salt stress condition, osmoticstress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UVirradiation.

Salinity Tolerance Assay—

Transgenic plants with tolerance to high salt concentrations areexpected to exhibit better germination, seedling vigor or growth in highsalt. Salt stress can be effected in many ways such as, for example, byirrigating the plants with a hyperosmotic solution, by cultivating theplants hydroponically in a hyperosmotic growth solution (e.g., Hoaglandsolution), or by culturing the plants in a hyperosmotic growth medium[e.g., 50% Murashige-Skoog medium (MS medium)]. Since different plantsvary considerably in their tolerance to salinity, the salt concentrationin the irrigation water, growth solution, or growth medium can beadjusted according to the specific characteristics of the specific plantcultivar or variety, so as to inflict a mild or moderate effect on thephysiology and/or morphology of the plants (for guidelines as toappropriate concentration see, Bernstein and Kafkafi, Root Growth UnderSalinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, EshelA and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, andreference therein).

For example, a salinity tolerance test can be performed by irrigatingplants at different developmental stages with increasing concentrationsof sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl)applied from the bottom and from above to ensure even dispersal of salt.Following exposure to the stress condition the plants are frequentlymonitored until substantial physiological and/or morphological effectsappear in wild type plants. Thus, the external phenotypic appearance,degree of wilting and overall success to reach maturity and yieldprogeny are compared between control and transgenic plants. Quantitativeparameters of tolerance measured include, but are not limited to, theaverage wet and dry weight, the weight of the seeds yielded, the averageseed size and the number of seeds produced per plant. Transformed plantsnot exhibiting substantial physiological and/or morphological effects,or exhibiting higher biomass than wild-type plants, are identified asabiotic stress tolerant plants.

Osmotic Tolerance Test—

Osmotic stress assays (including sodium chloride and mannitol assays)are conducted to determine if an osmotic stress phenotype was sodiumchloride-specific or if it was a general osmotic stress relatedphenotype. Plants which are tolerant to osmotic stress may have moretolerance to drought and/or freezing. For salt and osmotic stressgermination experiments, the medium is supplemented for example with 50mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol, 500 mMsorbitol or 15 g (grams) PEG [Polyethylene Glycol 8000].

Drought Tolerance Assay/Osmoticum Assay—

Tolerance to drought is performed to identify the genes conferringbetter plant survival after acute water deprivation. To analyze whetherthe transgenic plants are more tolerant to drought, an osmotic stressproduced by the non-ionic osmolyte sorbitol in the medium can beperformed. Control and transgenic plants are germinated and grown inplant-agar plates for 4 days, after which they are transferred to platescontaining 500 mM sorbitol. The treatment causes growth retardation,then both control and transgenic plants are compared, by measuring plantweight (wet and dry), yield, and by growth rates measured as time toflowering.

Conversely, soil-based drought screens are performed with plantsoverexpressing the polynucleotides detailed above. Seeds from controlArabidopsis plants, or other transgenic plants overexpressing thepolypeptide of the invention are germinated and transferred to pots.Drought stress is obtained after irrigation is ceased accompanied byplacing the pots on absorbent paper to enhance the soil-drying rate.Transgenic and control plants are compared to each other when themajority of the control plants develop severe wilting. Plants arere-watered after obtaining a significant fraction of the control plantsdisplaying a severe wilting. Plants are ranked comparing to controls foreach of two criteria: tolerance to the drought conditions and recovery(survival) following re-watering.

Cold Stress Tolerance—

To analyze cold stress, mature (25 day old) plants are transferred to 4°C. chambers for 1 or 2 weeks, with constitutive light. Later on plantsare moved back to greenhouse. Two weeks later damages from chillingperiod, resulting in growth retardation and other phenotypes, arecompared between both control and transgenic plants, by measuring plantweight (wet and dry), and by comparing growth rates measured as time toflowering, plant size, yield, and the like.

Heat Stress Tolerance—

Heat stress tolerance is achieved by exposing the plants to temperaturesabove 34° C. for a certain period. Plant tolerance is examined aftertransferring the plants back to 22° C. for recovery and evaluation after5 days relative to internal controls (non-transgenic plants) or plantsnot exposed to neither cold or heat stress.

Water Use Efficiency—

can be determined as the biomass produced per unit transpiration. Toanalyze WUE, leaf relative water content can be measured in control andtransgenic plants. Fresh weight (FW) is immediately recorded; thenleaves are soaked for 8 hours in distilled water at room temperature inthe dark, and the turgid weight (TW) is recorded. Total dry weight (DW)is recorded after drying the leaves at 60° C. to a constant weight.Relative water content (RWC) is calculated according to the followingFormula IV:RWC=[(FW−DW)/(TW−DW)]×100  Formula IV

Thus, the invention is of high agricultural value for promoting theyield of commercially desired crops (e.g., biomass of vegetative organsuch as poplar wood, or reproductive organ such as number of seeds orseed biomass) under normal or growth-limiting conditions (e.g.,nitrogen-deficient conditions, abiotic stress).

Any of the transgenic plants described hereinabove or parts thereof maybe processed to produce a feed, meal, protein or oil preparation, suchas for ruminant animals.

The transgenic plants described hereinabove, which exhibit an increasedoil content can be used to produce plant oil (by extracting the oil fromthe plant).

The plant oil (including the seed oil and/or the vegetative portion oil)produced according to the method of the invention may be combined with avariety of other ingredients. The specific ingredients included in aproduct are determined according to the intended use. Exemplary productsinclude animal feed, raw material for chemical modification,biodegradable plastic, blended food product, edible oil, biofuel,cooking oil, lubricant, biodiesel, snack food, cosmetics, andfermentation process raw material. Exemplary products to be incorporatedto the plant oil include animal feeds, human food products such asextruded snack foods, breads, as a food binding agent, aquaculturefeeds, fermentable mixtures, food supplements, sport drinks, nutritionalfood bars, multi-vitamin supplements, diet drinks, and cereal foods.

According to some embodiments of the invention, the oil comprises a seedoil and/or a vegetative portion oil.

According to some embodiments of the invention, the plant cell forms apart of a plant.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having”and their conjugates mean “including but not limited to”.

The term “consisting of means “including and limited to”.

The term “consisting essentially of” means that the composition, methodor structure may include additional ingredients, steps and/or parts, butonly if the additional ingredients, steps and/or parts do not materiallyalter the basic and novel characteristics of the claimed composition,method or structure.

As used herein, the singular form “a”, “an” and “the” include pluralreferences unless the context clearly dictates otherwise. For example,the term “a compound” or “at least one compound” may include a pluralityof compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention maybe presented in a range format. It should be understood that thedescription in range format is merely for convenience and brevity andshould not be construed as an inflexible limitation on the scope of theinvention. Accordingly, the description of a range should be consideredto have specifically disclosed all the possible subranges as well asindividual numerical values within that range. For example, descriptionof a range such as from 1 to 6 should be considered to have specificallydisclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numberswithin that range, for example, 1, 2, 3, 4, 5, and 6. This appliesregardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to includeany cited numeral (fractional or integral) within the indicated range.The phrases “ranging/ranges between” a first indicate number and asecond indicate number and “ranging/ranges from” a first indicate number“to” a second indicate number are used herein interchangeably and aremeant to include the first and second indicated numbers and all thefractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniquesand procedures for accomplishing a given task including, but not limitedto, those manners, means, techniques and procedures either known to, orreadily developed from known manners, means, techniques and proceduresby practitioners of the chemical, pharmacological, biological,biochemical and medical arts.

It is appreciated that certain features of the invention, which are, forclarity, described in the context of separate embodiments, may also beprovided in combination in a single embodiment. Conversely, variousfeatures of the invention, which are, for brevity, described in thecontext of a single embodiment, may also be provided separately or inany suitable subcombination or as suitable in any other describedembodiment of the invention. Certain features described in the contextof various embodiments are not to be considered essential features ofthose embodiments, unless the embodiment is inoperative without thoseelements.

Various embodiments and aspects of the present invention as delineatedhereinabove and as claimed in the claims section below find experimentalsupport in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with theabove descriptions illustrate some embodiments of the invention in a nonlimiting fashion.

Generally, the nomenclature used herein and the laboratory proceduresutilized in the present invention include molecular, biochemical,microbiological and recombinant DNA techniques. Such techniques arethoroughly explained in the literature. See, for example, “MolecularCloning: A laboratory Manual” Sambrook et al., (1989); “CurrentProtocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed.(1994); Ausubel et al., “Current Protocols in Molecular Biology”, JohnWiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide toMolecular Cloning”, John Wiley & Sons, New York (1988); Watson et al.,“Recombinant DNA”, Scientific American Books, New York; Birren et al.(eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, ColdSpring Harbor Laboratory Press, New York (1998); methodologies as setforth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis,J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-IIIColigan J. E., ed. (1994); Stites et al. (eds), “Basic and ClinicalImmunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994);Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W.H. Freeman and Co., New York (1980); available immunoassays areextensively described in the patent and scientific literature, see, forexample, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578;3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533;3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521;“Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic AcidHybridization” Hames, B. D., and Higgins S. J., eds. (1985);“Transcription and Translation” Hames, B. D., and Higgins S. J., Eds.(1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “ImmobilizedCells and Enzymes” IRL Press, (1986); “A Practical Guide to MolecularCloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317,Academic Press; “PCR Protocols: A Guide To Methods And Applications”,Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategiesfor Protein Purification and Characterization—A Laboratory CourseManual” CSHL Press (1996); all of which are incorporated by reference asif fully set forth herein. Other general references are providedthroughout this document. The procedures therein are believed to be wellknown in the art and are provided for the convenience of the reader. Allthe information contained therein is incorporated herein by reference.

Example 1 Identifying Genes which Increase Nitroge Use Efficiency,Fertilizer Use Efficiency, Yield, Oil Content, Biomass and/or AbioticStress Tolerance

Genes which can increase nitrogen use efficiency (NUE), fertilizer useefficiency (FUE), yield, oil content, biomass and/or abiotic stresstolerance (ABST) were identified using several data mining andbioinformatics tools.

All nucleotide sequence datasets used here were originated from publiclyavailable databases. Sequence data from 76 different plant species wasintroduced into a single, comprehensive database. Other information ongene expression, protein annotation, enzymes and pathways were alsoincorporated. Major databases used include:

Genomes

-   -   Arabidopsis genome [TAIR genome version 6 (Hypertext Transfer        Protocol://World Wide Web (dot) arabidopsis (dot) org/)]    -   Rice genome [IRGSP build 4.0 (Hypertext Transfer Protocol://rgp        (dot) dna (dot) affrc (dot) go (dot) jp/IRGSP/)].    -   Poplar [Populus trichocarpa release 1.1 from JGI (assembly        release v1.0) (Hypertext Transfer Protocol://World Wide Web        (dot) genome (dot) jgi-psf (dot) org/)]    -   Brachypodium [JGI 4× assembly, Hypertext Transfer        Protocol://World Wide Web (dot) brachpodium (dot) org)]    -   Soybean [DOE-JGI SCP, version Glyma0 (Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)]    -   Grape [French-Italian Public Consortium for Grapevine Genome        Characterization grapevine genome (Hypertext Transfer        Protocol://World Wide Web (dot) genoscope (dot) cns (dot) fr/)]    -   Castobean [TIGR/J Craig Venter Institute 4× assembly [(Hypertext        Transfer Protocol://msc (dot) jcvi (dot) org/r_communis]    -   Sorghum [DOE-JGI SCP, version Sbi1 [Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)].    -   Partially assembled genome of Maize [Hypertext Transfer        Protocol://maizesequence (dot) org/]

Expressed EST and mRNA Sequences were Extracted from the FollowingDatabases:

-   -   GenBank (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/Genbank/).    -   RefSeq (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/RefSeq/).    -   TAIR (Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/).

Protein and Pathway Databases

-   -   Uniprot (Hypertext Transfer Protocol://World Wide Web (dot)        expasy (dot) uniprot (dot) org/).    -   AraCyc (Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/biocyc/index (dot) jsp).    -   ENZYME (Hypertext Transfer Protocol://expasy (dot) org/enzyme/).

Microarray Datasets were Downloaded from:

-   -   GEO (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/geo/)    -   TAIR (Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/).    -   Proprietary cotton fiber microarray data (PCT Publication No.        WO2008/075364)    -   Proprietary microarray data on Arabidopsis ecotypes (PCT        Publication No. WO2008/122980).

OTL (Quantitative Trailt Locus) Information

-   -   Gramene (Hypertext Transfer Protocol://World Wide Web (dot)        gramene (dot) org/qtl/).

Database assembly was performed to enable to build a wide, rich,reliable annotated and easy to analyze database comprised of publiclyavailable genomic mRNA, and ESTs DNA sequences, data from various cropsas well as gene expression, protein annotation and pathway data QTLs,and other relevant information.

Database assembly is comprised of a toolbox of gene refining,structuring, annotation and analysis tools enabling to construct atailored database for each gene discovery project. Gene refining andstructuring tools enable to reliably detect splice variants andantisense transcripts, generating understanding of various potentialphenotypic outcomes of a single gene. The capabilities of the “LEADS”platform of Compugen LTD for analyzing human genome have been confirmedand accepted by the scientific community [see e.g., “WidespreadAntisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21,379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300(5623), 1288-91; “Computational analysis of alternative splicing usingEST tissue information”, Xie H et al. Genomics 2002], and have beenproven most efficient in plant genomics as well.

EST Clustering and Gene Assembly—

For clustering and assembly Arabidopsis, rice, grape, sorghum,brachypodium and soybean genes the present inventors used “genomicLEADS” version. This tool allows most accurate clustering of ESTs andmRNA sequences on genome, and predicts gene structure as well asalternative splicing events and anti-sense transcription.

Gene Annotation—

Predicted genes and proteins were annotated as follows:

Sequences blast search [Hypertext Transfer Protocol://blast (dot) ncbi(dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] against all plant UniProt[Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/]was performed. Open reading frames of each putative transcript wereanalyzed and longest ORF with higher number of homologues was selectedas predicted protein of the transcript. The predicted proteins wereanalyzed by InterPro [Hypertext Transfer Protocol://World Wide Web (dot)ebi (dot) ac (dot) uk/interpro/].

Blast against proteins from AraCyc and ENZYME databases was used to mapthe predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using blastalgorithm [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov/Blast (dot) cgi] to validate the accuracy of thepredicted protein sequence, and for efficient detection of orthologs.

Gene Expression Profiling—

Few data sources were exploited for gene expression profiling, namelymicroarray data and digital expression profile (as mentioned above).According to gene expression profile, a correlation analysis wasperformed to identify genes which are co-regulated under differentdevelopmental stages and environmental conditions.

Publicly available microarray datasets were downloaded from NCBI GEOsites, renormalized, and integrated into the database. Expressionprofile was one of the most important resource data for identifyinggenes important for NUE, ABST, yield, biomass increment and/or FUE.Moreover, when homolog genes from different crops were found to beassociated with increase of NUE, ABST, FUE, biomass, yield or oilcontent, the genes were marked as “highly predictive” to improve thetrait.

A digital expression profile summary was compiled for each clusteraccording to all keywords included in the sequence records comprisingthe cluster. Digital expression, also known as electronic Northern Blot,is a tool that displays virtual expression profile based on the ESTsequences forming the gene cluster. The tool can provide the expressionprofile of a cluster in terms of plant anatomy (e.g. tissues/organs inwhich the gene is expressed), developmental stage (the developmentalstages at which a gene can be found) and profile of treatment (providesthe physiological conditions under which a gene is expressed such asdrought, cold, pathogen infection, etc). Given a random distribution ofESTs in the different clusters, the digital expression provides aprobability value that describes the probability of a cluster having atotal of N ESTs to contain X ESTs from a certain collection oflibraries. For the probability calculations the following parameterswere taken into consideration: a) the number of ESTs in the cluster; b)the number of ESTs of the implicated and related libraries; and c) theoverall number of ESTs available, representing the species. Therebyclusters with low probability values are highly enriched with ESTs fromthe group of libraries of interest indicating a specialized expression.

The results of the digital and microarray gene expression data areprovided in Tables 1-19, hereinbelow.

Below are summarized the key criteria used to select the genes whichexpression thereof in a plant can be used to increase NUE, FUE, biomass,yield, oil content and ABST. The overexpression Fold (“Fold”) iscalculated as the ratio between the number of ESTs found in a gene or anorthologue group for a certain category (“Keyword”) and the number ofexpected ESTs according to a normal distribution. A probabilistic value(P-value) was estimated for the calculated overexpression folds. Geneswere selected based on the results presented in Tables 1-19 below andother computational filtering combined with manual curation as detailedbelow.

NUE242, NUE244, NUE234, NUE239, NUE240, NUE514, NUE523, NUE533, NUE538,NUE548, NUE549, NUE241, NUE235, NUE251, NUE587 and NUE582 were selectedsince they are highly expressed in roots and under nutrient deficientconditions (as shown in Tables 1 and 2, hereinbelow).

TABLE 1 Digital expression of NUE242, NUE244, NUE234, NUE239, NUE240,NUE514, NUE523, NUE533, NUE538, NUE548, NUE549, NUE241, NUE235, NUE251,NUE587 and NUE582 in different tissues Anatomy germinating seed rootseedling shoot Genes fold p-value fold p-value fold p-value fold p-valueNUE242 10.57 2.68E−12 1.00 5.47E−01 NUE244 1.00 4.48E−02 3.00 1.06E−031.40 1.67E−01 2.00 2.03E−01 NUE234 6.89 1.80E−24 NUE239 7.26 1.87E−21NUE240 12.70 4.65E−40 NUE514 1.97 2.69E−01 2.95 7.95E−60 0.78 1 0.399.99E−01 NUE523 2.15 1.17E−05 1.11 4.45E−01 1.33 1.31E−01 NUE533 2.965.39E−04 0.76 8.43E−01 NUE538 3.47 1.05E−06 0.96 6.20E−01 NUE548 1.721.06E−02 0.65 8.48E−01 0.60 9.41E−01 NUE549 1.51 7.86E−06 2.52 7.38E−130.19 1 NUE241 3.32 7.66E−03 1.00 4.05E−01 0.88 6.88E−01 NUE235 1.002.95E−02 4.94 1.12E−06 0.48 9.46E−01 NUE251 2.72 3.33E−05 NUE587 2.392.20E−02 2.56 1.10E−01 NUE582 2.00 6.00E−08 1.19 2.80E−01 1.89 7.60E−06

Table 1. Digital expression of the indicated genes in germinating seed,root, seedling and shoots. Provided are the fold increase and thecalculated p-values. Results were considered statistically significantif the p-value was lower than 0.05. Blank cells indicate that either thegene is not expressed or data is not available.

TABLE 2 Digital expression of NUE242, NUE244, NUE234, NUE239, NUE240,NUE514, NUE523, NUE533, NUE538, NUE548, NUE549, NUE241, NUE235, NUE251,NUE587 and NUE582 under different growth conditions Treatment nutrientdrought etiolation heat stress deficiencies watelogging Genes foldp-value fold p-value fold p-value fold p-value fold p-value NUE242 1.004.69E−02 5.00 8.06E−06 NUE244 4.00 1.40E−02 7.00 2.93E−06 1.00 1.50E−01NUE234 3.00 2.51E−03 2.93 1.71E−02 NUE239 8.00 5.17E−11 17.36 2.11E−27NUE240 4.00 1.44E−05 26.09 6.02E−35 NUE514 0.14 1 0.49 9.96E−01 1.165.14E−01 5.75 1.26E−38 3.54 1.95E−04 NUE523 1.53 1.07E−01 4.35 5.73E−041.94 2.76E−01 NUE533 1.00 6.19E−01 4.00 3.57E−03 NUE538 1.69 2.10E−017.00 2.32E−06 5.00 2.30E−05 NUE548 0.76 7.80E−01 9.60 1.24E−09 NUE5491.91 8.07E−07 4.08 6.20E−12 9.58 8.54E−30 NUE241 3.00 8.97E−03 NUE2352.00 1.50E−01 6.00 2.61E−06 NUE251 1.89 9.79E−02 3.00 4.85E−02 8.002.04E−08 NUE587 4.50 1.73E−03 3.00 4.71E−03 NUE582 0.63 9.51E−01 3.204.00E−02 0.97 6.10E−01

Table 2. Digital expression of the indicated genes under drought,etiolation, heat stress, nutrient deficiencies and waterlogging.Provided are the fold increase and the calculated p-values. Results wereconsidered statistically significant if the p-value was lower than 0.05.Blank cells indicate that either the gene is not expressed or data isnot available.

NUE229, NUE248, NUE254, NUE542, NUE562, NUE237, NUE221, NUE585 andNUE588 were selected because of their high expression in roots and underdrought stress conditions (as shown in Tables 3 and 4, below).

TABLE 3 Digital expression of NUE229, NUE248, NUE254, NUE542, NUE562,NUE237, NUE221, NUE585 and NUE588 in different tissues Anatomy leaf seedroot seedling shoot Genes fold p-value fold p-value fold p-value foldp-value fold p-value NUE229 4.64 2.79E−04 NUE248 1.19 5.06E−01 3.566.36E−03 NUE254 2.26 1.35E−02 7.90 5.32E−22 0.33 9.53E−01 1.55 1.46E−01NUE542 4.22 9.75E−04 NUE562 2.75 2.40E−02 3.32 3.79E−08 0.71 9.32E−010.62 9.66E−01 NUE237 0.50 9.00E−01 5.35 5.22E−11 1.21 2.97E−01 0.509.67E−01 NUE221 4.15 2.33E−04 0.63 8.74E−01 1.41 2.48E−01 NUE585 2.001.20E−01 6.00 1.34E−04 NUE588 0.99 6.00E−01 2.56 7.16E−05 0.65 9.64E−011.30 1.29E−01

Table 3. Digital expression of the indicated genes in leaf, seed, root,seedling and shoots. Provided are the fold increase and the calculatedp-values. Results were considered statistically significant if thep-value was lower than 0.05. Blank cells indicate that either the geneis not expressed or data is not available.

TABLE 4 Digital expression of NUE229, NUE248, NUE254, NUE542, NUE562,NUE237, NUE221 and NUE588 under different growth conditions Treatmentcold drought etiolation salinity Genes fold p-value fold p-value foldp-value fold p-value NUE229 4.00 6.53E−03 NUE248 4.00 6.02E−03 NUE2543.13 1.29E−02 1.00 3.67E−01 NUE542 3.00 3.50E−02 6.00 1.61E−10 NUE5620.70 0.760127 2.75 3.66E−02 0.98 0.57666 4.35 2.80E−03 NUE237 6.003.30E−04 NUE221 4.00 1.38E−03 1.60 0.28739 NUE585 2.00 5.13E−02 NUE5882.10 0.173185 2.73 3.76E−02 1.39 0.185271 0.72 7.53E−01

Table 4. Digital expression of the indicated genes under cold, drought,etiolation and salinity. Provided are the fold increase and thecalculated p-values. Results were considered statistically significantif the p-value was lower than 0.05. Blank cells indicate that either thegene is not expressed or data is not available.

NUE252 and MAB106, NUE265, NUE553, NUE513, NUE579, NUE580, NUE256,NUE227 and NUE223 were selected because of their high expression underetiolation growth conditions (as shown in Table 5).

TABLE 5 Digital expression of NUE252, MAB106, NUE265, NUE553, NUE513,NUE579, NUE580, NUE256, NUE227 and NUE223 under different growthconditions Treatment drought etiolation heat heavy metal Genes foldp-value fold p-value fold p-value fold p-value NUE252 1.28 4.2E−01 5.672.0E−11 MAB106 0.49 8.7E−01 10.17 5.2E−71 NUE265 1.90 4.9E−02 4.002.6E−03 2.26 7.2E−02 NUE553 1.92 4.4E−02 NUE513 1.05 5.7E−01 3.751.5E−04 NUE579 0.27 9.8E−01 3.18 1.9E−05 NUE580 1.00 5.6E−01 3.163.8E−02 NUE256 1.84 5.9E−02 2.03 9.9E−03 3.43 4.7E−03 NUE227 4.743.4E−03 NUE223 1.40 4.19E−01  4.17 5.6E−09

Table 5. Digital expression of the indicated genes under drought,etiolation, heat and heavy metal. Provided are the fold increase and thecalculated p-values. Results were considered statistically significantif the p-value was lower than 0.05. Note the high expression of NUE252and MAB106 under etiolation. Blank cells indicate that either the geneis not expressed or data is not available.

TABLE 6 Digital expression of NUE252, MAB106, NUE265, NUE553, NUE513,NUE579, NUE580, NUE256, NUE227 and NUE223 under different growthconditions Treatment salinity oxidative stress waterlogging Genes foldp-value fold p-value fold p-value NUE252 MAB106 NUE265 3.00 6.3E−02NUE553 NUE513 NUE579 NUE580 NUE256 2.96 8.2E−02 NUE227 NUE223 2.858.91E−02  2.00 2.31E−02

Table 6. Digital expression of the indicated genes under salinity,oxidative stress and waterlogging. Provided are the fold increase andthe calculated p-values. Results were considered statisticallysignificant if the p-value was lower than 0.05. Blank cells indicatethat either the gene is not expressed or data is not available.

NUE224, NUE230, NUE255, NUE245, NUE237, NUE233, NUE231, NUE228, NUE225and NUE249 were selected because of their high expression in roots andexpressed when treated with plant hormones intrinsically related toplant growth and development (as shown in Tables 7, 8 and 9).

TABLE 7 Digital expression of NUE224, NUE230, NUE255, NUE245, NUE237,NUE233, NUE231, NUE228, NUE225 and NUE249 in different tissues Anatomyleaf callus root seedling shoot Genes fold p-value fold p-value foldp-value fold p-value fold p-value NUE224 1.20 4.0E−01 0.49 9.9E−01 7.264.1E−30 1.64 8.4E−03 1.04 5.0E−01 NUE230 0.71 8.3E−01 1.35 1.4E−02 2.767.1E−09 0.59 1.0E+00 1.16 2.1E−01 NUE255 4.00 3.3E−03 NUE245 1.482.5E−01 0.32 1.0E+00 2.14 4.4E−03 1.03 4.8E−01 1.53 3.2E−02 NUE237 0.478.8E−01 1.39 1.0E−01 5.12 1.3E−10 1.14 3.7E−01 0.47 9.8E−01 NUE233 1.734.4E−02 4.19 9.9E−05 0.95 6.2E−01 1.28 3.3E−01 NUE231 0.75 7.8E−01 8.664.6E−10 0.30 9.7E−01 NUE228 0.17 1.0E+00 2.29 2.4E−12 4.75 3.2E−23 0.131.0E+00 NUE225 11.25 0 2.41 1.0E−14 0.10 1.0E+00 NUE249 5.78 4.17E−05 

Table 7. Digital expression of the indicated genes in leaf, callus,root, seedling and shoot. Provided are the fold increase and thecalculated p-values. Results were considered statistically significantif the p-value was lower than 0.05. Blank cells indicate that either thegene is not expressed or data is not available.

TABLE 8 Digital expression of NUE224, NUE230, NUE255, NUE245, NUE237,NUE233, NUE231, NUE228, NUE225 and NUE249 under different growthconditions and treatments Treatment plant development hormones droughtetiolation Genes fold p-value fold p-value fold p-value NUE224 4.751.7E−06 1.51 1.4E−01 NUE230 2.74 2.1E−04 0.31 9.6E−01 NUE255 4.001.4E−04 NUE245 2.67 1.1E−02 1.28 4.6E−01 NUE237 4.26 5.9E−04 6.004.2E−04 NUE233 11.74 2.5E−10 NUE231 10.00 3.4E−10 NUE228 4.48 3.0E−09NUE225 3.45 3.6E−07 NUE249 2.00 3.0E−02

Table 8. Digital expression of the indicated genes under plantdevelopment hormones, drought and etiolation. Provided are the foldincrease and the calculated p-values. Results were consideredstatistically significant if the p-value was lower than 0.05. Blankcells indicate that either the gene is not expressed or data is notavailable.

TABLE 9 Digital expression of NUE224, NUE230, NUE255, NUE245, NUE237,NUE233, NUE231, NUE228, NUE225 and NUE249 under different growthtreatments Treatment waterlogging photoperiod response salinity Genesfold p-value fold p-value fold p-value NUE224 NUE230 1.26 4.3E−01 NUE255NUE245 2.00 2.7E−02 0.87 6.9E−01 NUE237 NUE233 NUE231 NUE228 NUE22521.00 3.4E−26 28.53 5.6E−82 NUE249 2.00 4.0E−03Table 9. Digital expression of the indicated genes under waterlogging,photoperiod response and salinity. Provided are the fold increase andthe calculated p-values. Results were considered statisticallysignificant if the p-value was lower than 0.05. Blank cells indicatethat either the gene is not expressed or data is not available.

NUE268, NUE574 and NUE575 were selected because of their high expressionin callus (a tissue with high cell division rate) and induced whentreated with plant growth and development related hormones (as shown inTable 10, below).

TABLE 10 Digital expression of NUE268, NUE574 and NUE575 in varioustissues and under different conditions and treatments NUE268 NUE574NUE575 Anatomy leaf fold 0.84 1.24 p-value 0.8 4.8E−01 callus fold 2.372.28 2.47 p-value 6.0E−19 2.0E−04 2.5E−07 root fold 0.41 0.31 1.20p-value 1 9.7E−01 3.8E−01 seedling fold 0.34 1.23 0.45 p-value 1 3.1E−019.9E−01 shoot fold 0.59 0.16 0.91 p-value 9.9E−01 1.0E+00 6.6E−01Treatment plant fold 4.46 2.80 1.84 development p-value 1.4E−12 5.5E−021.7E−01 hormones drought fold 2.00 p-value 1.7E−01 etiolation fold 0.200.35 0.23 p-value 1.0E+00 9.5E−01 9.9E-01 waterlogging fold p-valuephotoperiod fold 3.32 response p-value 3.4E−02 salinity fold 1.00p-value 4.3E−01Table 10. Digital expression of the indicated genes in various tissues(leaf, callus, root, seedling and shoot) and under various treatment orconditions (plant development hormones, drought, etiolation,waterlogging, photoperiod response and salinity. Provided are the foldincrease and the calculated p-values. Results were consideredstatistically significant if the p-value was lower than 0.05. Note thesignificant fold expression in callus and under plant developmenthormones.

CT75, CT7, CT76, CT71, CT74, CT11, CT20, CT81, CT22, CT82, CT3, CT40,CT1, CT6, CT27, CT2, NUE269, NUE545 and NUE544, were selected based ontheir high expression in cotton fiber, which formation is stronglyrelated to cell elongation (Tables 11 and 12 below) and therefore areexpected to have a positive effect on root development under normalconditions, nitrogen deficient conditions, fertilizer shortage and/orwater deficiencies conditions as well as for increasing oil content.

TABLE 11 Digital expression of CT75, CT7, CT76, CT71, CT74, CT11, CT20,CT81, CT22, CT82, CT3, CT40, CT1, CT6, CT27, CT2, NUE269, NUE545 andNUE544 in different tissues Anatomy cotton fiber fruit seed root GeneName fold p-value fold p-value fold p-value fold p-value CT75 1.639.3E−13 CT7 1.65 6.3E−16 CT76 1.21 1.9E−01 CT71 1.6 1.3E−28 CT74 1.682.4E−89 CT11 1.49 4.4E−04 CT20 1.68 1.1E−14 0.6 8.1E−01 CT81 1.379.8E−04 CT22 0.92 7.4E−01 CT82 1.31 3.6E−01 CT3 1.87 1.4E−14 CT40 1.271.9E−03 0.59 8.2E−01 CT1 1.53 2.4E−09 CT6 1.46 6.3E−09 CT27 0.65 9.0E−01CT2 1.43 1.7E−03 NUE269 1.50 2.5E−02 NUE545 1.39 4.6E−03 1 4.5E−01NUE544 1.73 1.5E−03Table 11. Digital expression of the indicated genes in cotton fibers,fruit, seed and root. Provided are the fold increase and the calculatedp-values. Results were considered statistically significant if thep-value was lower than 0.05. Note the significant fold expression incotton fiber. Blank cells indicate that either the gene is not expressedor data is not available.

TABLE 12 Digital expression of CT75, CT7, CT76, CT71, CT74, CT11, CT20,CT81, CT22, CT82, CT3, CT40, CT1, CT6, CT27, CT2, NUE269, NUE545 andNUE544 Anatomy seedling stem leaf Gene Name fold p-value fold p-valuefold p-value CT75 CT7 0.08 1 0.44 9.0E−01 CT76 CT71 0.17 1 CT74 0.17 1CT11 CT20 0.55 0.97 CT81 1.6 0.08 CT22 CT82 CT3 CT40 0.52 0.86 CT1 0.540.97 CT6 0.17 0.99 CT27 CT2 0.21 0.99 NUE269 NUE545 0.63 8.0E−01 NUE5440.6 8.3E−01Table 12. Digital expression of the indicated genes in seedling, stemand leaf. Provided are the fold increase and the calculated p-values.Results were considered statistically significant if the p-value waslower than 0.05. Blank cells indicate that either the gene is notexpressed or data is not available.

Plants growing under low nitrogen conditions or harsh drought conditionssuffer from severe leaf senescence. NUE525, NUE535, NUE565, NUE578,NUE515 and NUE591 were selected as genes highly induced in leaves andunder nutrient deficiencies of drought stress conditions (as shown inTables 13 and 14, below). In addition, NUE578 shows strong induction inplants affected by heat stress.

TABLE 13 Digital expression of NUE525, NUE535, NUE565, NUE578, NUE515and NUE591 in different tissues Anatomy Leaf Root Flower Callus Genesfold p-value fold p-value fold p-value fold p-value NUE525 2.54 4.4E−060.93 6.6E−01 0.28 1.0E+00 NUE535 8.10 1.4E−11 NUE565 4.78 3.3E−03 NUE5782.41 9.1E−04 0.20 1.0E+00 NUE515 3.67 2.2E−02 1.36 4.4E−01 1.00 3.7E−01NUE591 3.41 1.3E−02 1.40 3.6E−01 1.59 2.9E−01Table 13. Digital expression of the indicated genes in leaf, root,flower and callus. Provided are the fold increase and the calculatedp-values. Results were considered statistically significant if thep-value was lower than 0.05. Note the fold expression in leaf. Blankcells indicate that either the gene is not expressed or data is notavailable.

TABLE 14 Digital expression of NUE525, NUE535, NUE565, NUE578, NUE515and NUE591 under different conditions Treatment Nutrient deficiencyDrought Salinity Heat Genes fold p-value fold p-value fold p-value foldp-value NUE525 3.19 1.2E−02 0.54 9.4E−01 1.29 4.6E−01 NUE535 4.066.7E−03 NUE565 3.00 2.3E−02 NUE578 4.25 2.7E−05 1.00 4.0E−01 8.053.8E−08 NUE515 3.00 2.6E−02 NUE591 7.00 2.7E−05

Table 14. Digital expression of the indicated genes under nutrientdeficiency, drought, salinity and heat. Provided are the fold increaseand the calculated p-values. Results were considered statisticallysignificant if the p-value was lower than 0.05. Note the fold expressionunder nutrient deficiency and drought. Blank cells indicate that eitherthe gene is not expressed or data is not available.

NUE520, NUE521, NUE560, NUE563 and NUE573 were selected as genes thatcan improve seedling vigor under nitrogen stress conditions. NUE520,NUE521, NUE560 were selected as genes that are highly expressed in wholeseedlings and are highly induced under drought stress. NUE563 wasselected as a gene that is highly induced in seedling leaves and isinduced under salinity stress. NUE573 is induced in seedling roots andunder salinity stress (see Tables 15 and 16).

TABLE 15 Digital expression of NUE520, NUE521, NUE560, NUE563 and NUE573in different tissues Anatomy Leaf Root Flower Seedling Genes foldp-value fold p-value fold p-value fold p-value NUE520 1.80 8.0E−02 0.886.9E−01 1.34 1.4E−02 1.87 9.1E−05 NUE521 1.43 2.7E−01 1.06 4.4E−01 1.782.1E−02 NUE560 2.68 6.2E−02 0.66 8.2E−01 0.57 9.8E−01 3.67 1.2E−09NUE563 5.07 6.7E−05 0.28 9.8E−01 0.14 1.0E+00 5.30 4.7E−24 NUE573 0.171.0E+00 8.59 3.2E−47 2.00 3.0E−03Table 15. Digital expression of the indicated genes in leaf, root,flower and seedling. Provided are the fold increase and the calculatedp-values. Results were considered statistically significant if thep-value was lower than 0.05. Note the fold expression in leaf (NUE563),root (NUE573) and seedling (NUE520, NUE521, NUE560, NUE563 and NUE573).Blank cells indicate that either the gene is not expressed or data isnot available.

TABLE 16 Digital expression of NUE520, NUE521, NUE560, NUE563 and NUE573under different conditions Treatment Nutrient deficiencies Drought HeatSalinity Genes fold p-value fold p-value fold p-value fold p-valueNUE520 3.96 1.1E−03 8.00 5.5E−06 2.60 6.9E−02 NUE521 6.00 1.3E−04 1.004.5E−01 NUE560 5.00 5.9E−04 NUE563 3.00 2.4E−02 NUE573 1.73 1.3E−01 2.005.4E−02Table 16. Digital expression of the indicated genes under nutrientdeficiency, drought, heat and salinity. Provided are the fold increaseand the calculated p-values. Results were considered statisticallysignificant if the p-value was lower than 0.05. Note the fold expressionunder drought (NUE520, NUE521, NUE560) and salinity (NUE563 and NUE573).Blank cells indicate that either the gene is not expressed or data isnot available.

Seedlings and cell culture are fast growing tissues. Furthermore,emerging root seedlings elongate very fast to reach available water andnitrogen at deeper soils. NUE520, NUE211, NUE564 and NUE567 wereselected for their high expression in root seedlings and/or wholeseedlings, while NUE519 was selected for its high expression in rootseedlings and cell cultures (see Table 17).

TABLE 17 Digital expression of NUE520, NUE211, NUE564, NUE567, andNUE519 in different tissues NUE211 NUE564 NUE567 NUE519 Anatomy Leaffold 1.76 3.39 p-value 2.0E−01 2.5E−03 Cell suspension fold 0.24 8.00p-value 9.9E−01 8.4E−12 Root fold 1.91 3.50 6.11 3.21 p-value 4.6E−021.1E−03 6.9E−06 5.1E−05 Seedling fold 2.01   3.687807 p-value 1.2E−035.9E−03 Shoot fold 1.29 0.21 p-value 2.0E−01 1.0E+00

Table 17. Digital expression of the indicated genes in leaf, cellsuspension, root, seedling and shoot. Provided are the fold increase andthe calculated p-values. Results were considered statisticallysignificant if the p-value was lower than 0.05. Note the fold expressionin root (NUE211, NUE564, NUE567 and NUE519) and seedling (NUE211 andNUE564). Blank cells indicate that either the gene is not expressed ordata is not available.

NUE528, NUE571, NUE531 and NUE590 are induced by cold stress. Coldstress reduces plant photosynthesis and produces similar effect to thatobserved in plants growing under nitrogen deficiency (see Table 18).

TABLE 18 Digital expression of NUE528, NUE571, NUE531 and NUE590 underdifferent conditions Treatment Nutrient deficiencies Cold Heat SalinityDrought Genes fold p-value fold p-value fold p-value fold p-value foldp-value NUE528 2.47 0.08 3.00 5.2E−04 NUE571 7.24 5.8E−09 NUE531 6.004.5E−04 NUE590 1.00 3.9E−02 1 2.9E−01 1 2.9E−01

Table 18. Digital expression of the indicated genes under nutrientdeficiencies, cold, heat, salinity and drought. Provided are the foldincrease and the calculated p-values. Results were consideredstatistically significant if the p-value was lower than 0.05. Note thefold expression under nutrient deficiencies (NUE528) and cold (NUE528,571, 531 and 590). Blank cells indicate that either the gene is notexpressed or data is not available.

NUE206 was selected based on its digital expression analysis. It showedthat NUE206 is highly expressed in roots (2.4 fold p<0.05) andindications of being induced by cold (2.2 fold p<0.08). NUE208 andNUE210 are tomato genes that are expressed in fruit and during fruitripening, respectively. These stages are considered important formaintaining high cell turgor. NUE209 is a putative HB2 homeodomainprotein highly expressed in flower buds. It was selected as a gene thatbelongs to an orthologue group of genes that are highly induced by plantdevelopmental hormones such as auxins (5 fold p<0.002), and in tissuesthat maintain high cell turgor such as the fruit pulp (3 fold p<0.00098)and callus (2 fold p<0.0003). NUE246 was selected because of its highexpression in fruit pericarp (3.7 fold p<0.01) and because it is highlyinduced by drought (4 fold, p<0.0013). NUE516 is a putative Pto kinaseinteractor selected for its induction under drought conditions (3.2fold, p<0.03) and prior to flowering stage (2.0 fold p<0.02). NUE527 waschosen because of its expression in different nutrient deficiencies (3.7fold p<0.002) being mainly expressed under phosphate deficiency (4 fold,p<0.006). NUE547, which is a Putative Ca(2+)-dependent nuclease, wasselected as a gene induced in flowers during pre-anthesis stage (2.0fold p<0.04). NUE551 is an uncharacterized protein that was classifiedand chosen as a gene that is induced in flowers (2.6 fold p<0.007) andis involved in plant carbon metabolism (GO:0005975 carbohydratemetabolism). NUE554 was characterized as TBP-binding protein-like who isinduced in shoots (1.8 fold p<8e-09) during blister and/or milking grainfilling stage (3.4 fold p<1e-08). NUE583 is an uncharacterized proteinhighly expressed in flowers (2.5 fold p<0.006) and significantly inducedby cytokinins (4.0 fold p<2e-05). NUE584 is an unknown protein highlyinduced in shoots and roots (6.0 fold p<8e-07) and overrepresented undernutrient deficiency conditions (6.0 fold p<1e-08) and drought (3.0 foldp<0.03). NUE592 is an unknown protein induced by phosphate deficiency(2.0 fold p<0.05) and by stress related hormones (6.1 fold p<2E-05)

Other NUE and MAB genes were selected based on their induced expressionin different Microarrays experiments. The experiments selected from theGene Expression Omnibus (Hypertext Transfer Protocol://World Wide Web(dot) ncbi (dot) nlm (dot) nih (dot) gov/geo/) were abiotic stresses(drought, salinity) GSE6901, nitrogen deficiency GSE4409, cold GSE3326,rice atlas GSE6893, and auxin GSE3350. From TAIR (Hypertext TransferProtocol://World Wide Web (dot) arabidopsis (dot)org/servlets/Search?type=expr&search_action=new_search) the experimentson salinity 1007966888, osmoticum 1007966835, cold 1007966553 and ABAapplication 1007964750 were chosen, and from Nascarrays (HypertextTransfer Protocol://affymetrix (dot) arabidopsis (dot)info/narrays/experimentbrowse (dot) pI) an experiment on Nitrogendeficiency NASCARRAYS-136 was chosen. Furthermore, a Proprietary cottonfiber microarray data was used to detect the expression of the genes incotton fiber or root specifically (PCT Publication No: WO 2008/075364)

Based on the analysis of the microarray experiments described aboveNUE222 was selected because it is highly expressed under nitrogendeficiency, salinity and because it is strongly induced by ABA (seeTable 19, hereinbelow). NUE267 and NUE206 were selected as these genesare highly induced by salinity, cold and ABA. NUE212 is a cotton genespecifically expressed in roots. MAB52 was selected because it isinduced by drought. MAB53 was selected because it is induced by nitrogendeficiency and it is a functional orthologue of MAB106. NUE566 andNUE568 were selected for their high expression in leaves when comparedto their expression in roots). NUE570 was selected because it is highlyoverrepresented in EST's libraries of leaves (5 fold p<0.001) and isinduced by salinity in the microarray experiment. NUE540 is expressed inroots and is related to root hair cell differentiation (GO:0048765).NUE539, NUE543, NUE576 and NUE577 were selected for being highly inducedunder nitrogen deficiency. NUE577 was also selected for being inducedunder salinity and cold stress. NUE569 was selected for being inducedunder salinity and osmoticum conditions. NUE586 was selected for beinginducted when treated with the growth hormone auxin. NUE253 was selectedas a highly expressed gene under nitrogen deficiency and salinity andNUE593 was selected as a highly expressed gene under salinity conditions

TABLE 19 Microarray expression analysis of NUE222, NUE267, NUE206,NUE212, MAB52, MAB53, NUE539, NUE543, NUE576, NUE566, NUE568, NUE569,NUE570, NUE572, NUE581, NUE540, NUE586, NUE577, NUE253 and NUE593 FoldGene Fold Fold Fold Nitrogen Fold Fold Fold Fold Fold Name SalinityDrought Osmoticum Deficiency Cold ABA Roots Shoot Auxin NUE222 5.0 2.03.0 NUE267 3.0 4.4 3.5 NUE206 4.0 10.0 6.0 NUE212 12.0 MAB52 1.6 MAB531.9 NUE539 4.4 NUE543 2.1 NUE576 3.3 NUE566 2.3 NUE568 22.0 NUE569 1.51.6 NUE570 6.2 NUE572 1.2 2.1 NUE581 20.9 7.0 NUE540 2.0 NUE586 3.1NUE577 2.1 1.9 4.4 NUE253 1.8 1.6 NUE593 2.0

Table 19: Microarray expression analysis of the indicated genes undersalinity, drought, osmoticum, nitrogen deficiency, cold, ABA (abscisicacid) conditions and in roots, shoot and auxin. Blank cells indicatethat either the gene is not expressed.

NUE49, NUE50 and NUE102 are variants of previously described genes thatwere originally selected for yield and NUE improvement (PCT PublicationNo. WO2007/049275)

Overall 137 genes were identified to have a major impact on nitrogen useefficiency, fertilizer use efficiency, yield (e.g., seed yield, oilyield, grain quantity and/or quality), growth rate, vigor, biomass, oilcontent, abiotic stress tolerance and/or water use efficiency whenexpression thereof is increased in plants. The identified genes, theircurated polynucleotide and polypeptide sequences, as well as theirupdated sequences according to GenBank database are summarized in Table20, hereinbelow.

TABLE 20 Genes which affect nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, abioticstress tolerance and/or water use efficiency Polyn. Polyp. Gene NameCluster Name Organism SEQ ID NO: SEQ ID NO: CT1 cotton|gb164|AI725990cotton 1 138 CT11 cotton|gb164|AI725968 cotton 2 139 CT2cotton|gb164|AI727334 cotton 3 140 CT20 cotton|gb164|AI726497 cotton 4141 CT22 cotton|gb164|BG440027 cotton 5 142 CT27 cotton|gb164|AF336280cotton 6 143 CT3 cotton|gb164|AI725456 cotton 7 144 CT40cotton|gb164|BE052317 cotton 8 145 CT6 cotton|gb164|AI726479 cotton 9146 CT7 cotton|gb164|AI727027 cotton 10 147 CT71 cotton|gb164|AI725508cotton 11 148 CT74 cotton|gb164|AI725950 cotton 12 149 CT75cotton|gb164|AI726599 cotton 13 150 CT76 cotton|gb164|AI726155 cotton 14151 CT81 cotton|gb164|AI726693 cotton 15 152 CT82 cotton|gb164|BQ402794cotton 16 153 MAB106 barley|gb157.2|AL450627 barley 17 154 MAB52rice|gb157.2|AU070543 rice 18 155 MAB53 rice|gb157.2|BI805919 rice 19156 NUE102 maize|gb170|AI974922 maize 20 157 NUE206arabidopsis|gb165|AT4G24960 arabidopsis 21 158 NUE208tomato|gb164|BG124666 tomato 22 159 NUE209 tomato|gb164|BG134403 tomato23 160 NUE210 tomato|gb157|TOMTRALTAB tomato 24 161 NUE211rice|gb157.2|AU174544 rice 25 162 NUE212 cotton|gb164|CO081293 cotton 26163 NUE221 rice|gb157.2|BI305241 rice 27 164 NUE222arabidopsis|gb165|AT1G31820 arabidopsis 28 165 NUE223rice|gb157.2|AW069985 rice 29 166 NUE224 rice|gb157.2|AW155063 rice 30167 NUE225 rice|gb157.2|BE039221 rice 31 168 NUE227rice|gb157.2|AU056888 rice 32 169 NUE228 rice|gb157.2|AA753730 rice 33170 NUE229 maize|gb164|AW455682 maize 34 171 NUE230rice|gb157.2|AA749861 rice 35 172 NUE231 rice|gb157.2|AK108994 rice 36173 NUE233 rice|gb157.2|CB640732 rice 37 174 NUE234poplar|gb157.2|BU868634 poplar 38 175 NUE235 soybean|gb162|CA852963soybean 39 176 NUE237 rice|gb157.2|BI811377 rice 40 177 NUE239poplar|gb157.2|BU880014 poplar 41 178 NUE240 poplar|gb157.2|AJ407707poplar 42 179 NUE241 tomato|gb164|BG129806 tomato 43 180 NUE242tomato|gb164|BG791300 tomato 44 181 NUE244 soybean|gb162|CF808561soybean 45 182 NUE245 rice|gb157.2|AT003383 rice 46 183 NUE246grape|gb160|CF207859 grape 47 184 NUE248 maize|gb157|BG354535 maize 48185 NUE249 rice|gb157.2|AU029933 rice 49 186 NUE250rice|gb157.2|AK102239 rice 50 187 NUE251 sorghum|gb161.xeno|AI947781sorghum 51 188 NUE252 arabidopsis|gb165|AT1G58030 arabidopsis 52 189NUE253 rice|gb157.2|AF145730 rice 53 190 NUE254 maize|gb164|AI600563maize 54 191 NUE255 rice|gb157.2|CB000630 rice 55 192 NUE256wheat|gb154|TG_BE216912 wheat 56 193 NUE265 rice|gb157.2|BE039218 rice57 194 NUE267 arabidopsis|gb165|AT5G60680 arabidopsis 58 195 NUE268rice|gb157.2|AA750934 rice 59 196 NUE269 cotton|gb164|AI730085 cotton 60197 NUE49 maize|gb154|AW037179 maize 61 198 NUE50 maize|gb164|AW287760maize 62 199 NUE511 maize|gb157|AW360667 maize 63 200 NUE512arabidopsis|gb157.2|AT5G23460 arabidopsis 64 201 NUE513arabidopsis|gb157.2|AT3G26100 arabidopsis 65 202 NUE514soybean|gb162|SOYHPR soybean 66 203 NUE515 arabidopsis|gb165|AT1G44920arabidopsis 67 204 NUE515 arabidopsis|gb157.2|AT1G44920_P1 arabidopsis67 266 NUE516 arabidopsis|gb157.2|AT1G48210 arabidopsis 68 205 NUE519wheat|gb164|BE445396 wheat 69 206 NUE520 rice|gb157.2|BI305493 rice 70207 NUE521 rice|gb157.2|AU077950 rice 71 208 NUE523sorghum|gb161.xeno|AI901439 sorghum 72 209 NUE525sorghum|gb161.xeno|AW052978 sorghum 73 210 NUE527sorghum|gb161.xeno|AW055409 sorghum 74 211 NUE528sorghum|gb161.xeno|AI372194 sorghum 75 212 NUE531 rice|gb157.2|B1805136rice 76 213 NUE532 maize|gb164|AW054475 maize 77 214 NUE533soybean|gb166|AW350050 soybean 78 215 NUE535 sorghum|gb161.crp|BE599042sorghum 79 216 NUE536 maize|gb164|BQ279657 maize 80 217 NUE537barley|gb157.2|AJ234408 barley 81 218 NUE538 sorghum|gb161.xeno|AW923729sorghum 82 219 NUE539 rice|gb157.2|AW155216 rice 83 220 NUE540arabidopsis|gb157.2|AT1G13980 arabidopsis 84 221 NUE542arabidopsis|gb157.2|AT3G46280 arabidopsis 85 222 NUE543rice|gb157.2|AK063415 rice 86 223 NUE544 cotton|gb164|BQ412384 cotton 87224 NUE545 cotton|gb164|AI055737 cotton 88 225 NUE547sorghum|gb161.xeno|BI139559 sorghum 89 226 NUE548sorghum|gb161.xeno|BQ279657 sorghum 90 227 NUE549sorghum|gb161.xeno|AF019147 sorghum 91 228 NUE550 canola|gb161|EE559843canola 92 229 NUE551 barley|gb157.3|BE420701 barley 93 230 NUE553barley|gb157.3|BE421829 barley 94 231 NUE554 sorghum|gb161.xeno|AA011880sorghum 95 232 NUE560 rice|gb157.2|BE229552 rice 96 233 NUE562rice|gb157.2|BE039784 rice 97 234 NUE563 rice|gb157.2|AU057884 rice 98235 NUE564 maize|gb164|AI619269 maize 99 236 NUE565arabidopsis|gb157.2|AT5G15080 arabidopsis 100 237 NUE566arabidopsis|gb165|AT2G43700 arabidopsis 101 238 NUE567arabidopsis|gb165|AT1G60680 arabidopsis 102 239 NUE568arabidopsis|gb165|AT1G78450 arabidopsis 103 240 NUE569arabidopsis|gb165|AT2G03890 arabidopsis 104 241 NUE570arabidopsis|gb165|AT1G43910 arabidopsis 105 242 NUE571arabidopsis|gb157.2|AT1G47530 arabidopsis 106 243 NUE572arabidopsis|gb157.2|AT2G24240 arabidopsis 107 244 NUE573arabidopsis|gb165|AT4G15390 arabidopsis 108 245 NUE574rice|gb157.2|BI807603 rice 109 246 NUE575 rice|gb157.2|AU068829 rice 110247 NUE576 rice|gb157.2|AA752451 rice 111 248 NUE577arabidopsis|gb165|AT1G67800 arabidopsis 112 249 NUE578wheat|gb164|BE401454 wheat 113 250 NUE579 arabidopsis|gb165|AT1G70850arabidopsis 114 251 NUE580 arabidopsis|gb165|AT2G35880 arabidopsis 115252 NUE581 arabidopsis|gb165|AT1G12845 arabidopsis 116 253 NUE582sorghum|gb161.xeno|T18303 sorghum 117 254 NUE583 rice|gb157.2|AU172665rice 118 255 NUE584 sorghum|gb161.crp|AW923545 sorghum 119 256 NUE585arabidopsis|gb165|AT1G71900 arabidopsis 120 257 NUE586arabidopsis|gb165|AT1G72320 arabidopsis 121 258 NUE587sorghum|gb161.xeno|AW672541 sorghum 122 259 NUE588 rice|gb157.2|AA750816rice 123 260 NUE590 sorghum|gb161.xeno|AI622209 sorghum 124 261 NUE591sorghum|gb161.xeno|BE123399 sorghum 125 262 NUE592sorghum|gb161.xeno|AI901557 sorghum 126 263 NUE593arabidopsis|gb165|AT2G04066 arabidopsis 127 264 CT82cotton|gb164|BQ402794_T1 cotton 128 153 NUE102 maize|gb164|AI974922_T1maize 129 265 NUE211 rice|gb157.2|AU174544_T1 rice 130 162 NUE212cotton|gb164|CO081293_T1 cotton 131 163 NUE269 cotton|gb164|AI730085_T1cotton 132 197 NUE519 wheat|gb164|BE445396_T1 wheat 133 206 NUE535sorghum|gb161.xeno|BE599042_T1 sorghum 134 267 NUE537barley|gb157.2|AJ234408_T1 barley 135 218 NUE544cotton|gb164|BQ412384_T1 cotton 136 268 NUE584sorghum|gb161.xeno|AW923465_T1 sorghum 137 269

Table 20. Provided are polynucleotides (polyn.) and polypeptides(polyp.) which affect nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, abioticstress tolerance and/or water use efficiency of a plant.

Example 2 Identification of Homologues which Affect NUE, FUE, Yield,Growth Rate, Vigor, Biomass, Oil Content, ABST and WUE

The concepts of orthology and paralogy have been applied to functionalcharacterizations and classifications on the scale of whole-genomecomparisons. Orthologs and paralogs constitute two major types ofhomologs: The first evolved from a common ancestor by specialization,and the latter are related by duplication events. It is assumed thatparalogs arising from ancient duplication events are likely to havediverged in function while true orthologs are more likely to retainidentical function over evolutionary time.

To further investigate and identify putative ortholog genes of genesaffecting nitrogen use efficiency, fertilizer use efficiency, yield(e.g., seed yield, oil yield, biomass, grain quantity and/or quality),growth rate, vigor, biomass, oil content, abiotic stress toleranceand/or water use efficiency (presented in Table 20, above) all sequenceswere aligned using the BLAST (/Basic Local Alignment Search Tool/).Sequences sufficiently similar were tentatively grouped. These putativeorthologs were further organized under a Phylogram—a branching diagram(tree) assumed to be a representation of the evolutionary relationshipsamong the biological taxa. Putative ortholog groups were analyzed as totheir agreement with the phylogram and in cases of disagreements theseortholog groups were broken accordingly. Expression data was analyzedand the EST libraries were classified using a fixed vocabulary of customterms such as developmental stages (e.g., genes showing similarexpression profile through development with up regulation at specificstage, such as at the seed filling stage) and/or plant organ (e.g.,genes showing similar expression profile across their organs with upregulation at specific organs such as root). The annotations from allthe ESTs clustered to a gene were analyzed statistically by comparingtheir frequency in the cluster versus their abundance in the database,allowing the construction of a numeric and graphic expression profile ofthat gene, which is termed “digital expression”. The rationale of usingthese two complementary methods with methods of phenotypic associationstudies of QTLs, and phenotype expression correlation is based on theassumption that true orthologs are likely to retain identical functionover evolutionary time. These methods provide different sets ofindications on function similarities between two homologous genes,similarities in the sequence level—identical amino acids in the proteindomains and similarity in expression profiles.

The search and identification of homologous genes involves the screeningof sequence information available, for example, in public databases,which include but are not limited to the DNA Database of Japan (DDBJ),Genbank, and the European Molecular Biology Laboratory Nucleic AcidSequence Database (EMBL) or versions thereof or the MIPS database. Anumber of different search algorithms have been developed, including butnot limited to the suite of programs referred to as BLAST programs.There are five implementations of BLAST, three designed for nucleotidesequence queries (BLASTN, BLASTX, and TBLASTX) and two designed forprotein sequence queries (BLASTP and TBLASTN) (Coulson, Trends inBiotechnology: 76-80, 1994; Birren et al., Genome Analysis, I: 543,1997). Such methods involve alignment and comparison of sequences. TheBLAST algorithm calculates percent sequence identity and performs astatistical analysis of the similarity between the two sequences. Thesoftware for performing BLAST analysis is publicly available through theNational Centre for Biotechnology Information. Other such software oralgorithms are GAP, BESTFIT, FASTA and TFASTA. GAP uses the algorithm ofNeedleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find thealignment of two complete sequences that maximizes the number of matchesand minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis ofa gene family may be carried out using sequence similarity analysis. Toperform this analysis one may use standard programs for multiplealignments e.g. Clustal W. A neighbor-joining tree of the proteinshomologous to the genes of some embodiments of the invention may be usedto provide an overview of structural and ancestral relationships.Sequence identity may be calculated using an alignment program asdescribed above. It is expected that other plants will carry a similarfunctional gene (orthologue) or a family of similar genes and thosegenes will provide the same preferred phenotype as the genes presentedhere. Advantageously, these family members may be useful in the methodsof some embodiments of the invention. Example of other plants include,but not limited to, barley (Hordeum vulgare), Arabidopsis (Arabidopsisthaliana), maize (Zea mays), cotton (Gossypium), Oilseed rape (Brassicanapus), Rice (Oryza sativa), Sugar cane (Saccharum officinarum), Sorghum(Sorghum bicolor), Soybean (Glycine max), Sunflower (Helianthus annuus),Tomato (Lycopersicon esculentum) and Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology is preferably carriedout on a full-length sequence, but may also be based on a comparison ofcertain regions such as conserved domains. The identification of suchdomains would also be well within the realm of the person skilled in theart and would involve, for example, a computer readable format of thenucleic acids of some embodiments of the invention, the use of alignmentsoftware programs and the use of publicly available information onprotein domains, conserved motifs and boxes. This information isavailable in the PRODOM (Hypertext Transfer Protocol://World Wide Web(dot) biochem (dot) ucl (dot) ac (dot)uk/bsm/dbbrowser/protocol/prodomqry (dot) html), PIR (Hypertext TransferProtocol://pir (dot) Georgetown (dot) edu/) or Pfam (Hypertext TransferProtocol://World Wide Web (dot) sanger (dot) ac (dot) uk/Software/Pfam/)database. Sequence analysis programs designed for motif searching may beused for identification of fragments, regions and conserved domains asmentioned above. Preferred computer programs include, but are notlimited to, MEME, SIGNALSCAN, and GENESCAN.

A person skilled in the art may use the homologous sequences providedherein to find similar sequences in other species and other organisms.Homologues of a protein encompass, peptides, oligopeptides,polypeptides, proteins and enzymes having amino acid substitutions,deletions and/or insertions relative to the unmodified protein inquestion and having similar biological and functional activity as theunmodified protein from which they are derived. To produce suchhomologues, amino acids of the protein may be replaced by other aminoacids having similar properties (conservative changes, such as similarhydrophobicity, hydrophilicity, antigenicity, propensity to form orbreak a-helical structures or 3-sheet structures). Conservativesubstitution Tables are well known in the art [see for example Creighton(1984) Proteins. W.H. Freeman and Company]. Homologues of a nucleic acidencompass nucleic acids having nucleotide substitutions, deletionsand/or insertions relative to the unmodified nucleic acid in questionand having similar biological and functional activity as the unmodifiednucleic acid from which they are derived.

Table 21, hereinbelow, lists a summary of orthologous and homologoussequences of the polynucleotide sequences (SEQ ID NOs:1-137) andpolypeptide sequences (SEQ ID NOs:138-269) presented in Table 20, whichwere identified using BLAST (TBLASTN and BlastP programs) having atleast 80% identity to the selected polypeptides and which are expectedto posses the same role in NUE, ABST, FUE, WUE, biomass increment,growth rate increment, yield, vigor and/or oil content of plants.

TABLE 21 Homologues of the identified polynucleotides and polypeptideswhich affect nitrogen use efficiency, fertilizer use efficiency, yield,growth rate, vigor, biomass, oil content, abiotic stress toleranceand/or water use efficiency of a plant Homol- Polyn. Polyp. ogy to % SEQID SEQ SEQ ID Core cluster global Algo- NO: Cluster name Organism ID NO:name identity rithm 270 cacao|gb167|CU484898 cacao 1334 138cotton|gb164|AI725990 88.2 blastp 271 cotton|gb164|AI726705 cotton 1335138 cotton|gb164|AI725990 86.9 blastp 272 almond|gb157.2|AY947462 almond1336 139 cotton|gb164|AI725968 85.7 blastp 273 apple|gb157.3|CO415932apple 1337 139 cotton|gb164|AI725968 83.5 blastp 274 bean|gb167|CA902463bean 1338 139 cotton|gb164|AI725968 87.9 blastp 275 cacao|gb167|CU519200cacao 1339 139 cotton|gb164|AI725968 95.5 blastp 276citrus|gb166|CK936045 citrus 1340 139 cotton|gb164|AI725968 92.4 blastp277 cotton|gb164|AI728519 cotton 1341 139 cotton|gb164|AI725968 90.7blastp 278 grape|gb160|AF373604 grape 1342 139 cotton|gb164|AI72596886.2 blastp 279 lotus|gb157.2|AY770405 lotus 1343 139cotton|gb164|AI725968 85.7 blastp 280 medicago|gb157.2|BI311053 medicago1344 139 cotton|gb164|AI725968 87.4 blastp 281papaya|gb165|GFXEU141966X1 papaya 1345 139 cotton|gb164|AI725968 90.1blastp 282 poplar|gb170|BU882889 poplar 1346 139 cotton|gb164|AI72596887.6 blastp 283 poplar|gb170|CV256507 poplar 1347 139cotton|gb164|AI725968 83.9 blastp 284 prunus|gb167|AJ825116 prunus 1348139 cotton|gb164|AI725968 85.2 blastp 285 soybean|gb168|BE659913 soybean1349 139 cotton|gb164|AI725968 87.4 blastp 286 soybean|gb168|BE659915soybean 1350 139 cotton|gb164|AI725968 85.2 blastp 287spurge|gb161|DV143720 spurge 1351 139 cotton|gb164|AI725968 84.75tblastn 288 cotton|gb164|AI726482 cotton 1352 140 cotton|gb164|AI72733498.1 blastp 289 cacao|gb167|CU473257 cacao 1353 141cotton|gb164|AI726497 87.4 blastp 290 cotton|gb164|BF272326 cotton 1354141 cotton|gb164|AI726497 83.3 blastp 291 cotton|gb164|AI729672 cotton1355 144 cotton|gb164|AI725456 83.7 blastp 292 cotton|gb164|CB350460cotton 1356 145 cotton|gb164|BE052317 87.8 blastp 293cotton|gb164|DV437946 cotton 1357 145 cotton|gb164|BE052317 87.8 blastp294 cotton|gb164|AI726435 cotton 1358 146 cotton|gb164|AI726479 95.1blastp 295 cacao|gb167|CF972823 cacao 1359 148 cotton|gb164|AI72550881.4 blastp 296 cotton|gb164|AI725520 cotton 1360 148cotton|gb164|AI725508 81.8 blastp 297 cotton|gb164|BE054381 cotton 1361148 cotton|gb164|AI725508 85.4 blastp 298 cotton|gb164|AI726610 cotton1362 149 cotton|gb164|AI725950 86.8 blastp 299 cotton|gb164|AI731567cotton 1363 149 cotton|gb164|AI725950 96.4 blastp 300cotton|gb164|AI726627 cotton 1364 150 cotton|gb164|AI726599 96.4 blastp301 brachypodium|gb169|BE425417 brachypodium 1365 154barley|gb157.2|AL450627 84.7 blastp 302 leymus|gb166|EG388830 leymus1366 154 barley|gb157.2|AL450627 86.4 blastp 303pseudoroegneria|gb167|FF340314 pseudoroegneria 1367 154barley|gb157.2|AL450627 89.4 blastp 304 wheat|gb164|BE429931 wheat 1368154 barley|gb157.2|AL450627 89.4 blastp 305 switchgrass|gb167|DN142225switchgrass 1369 156 rice|gb157.2|BI805919 82.5 blastp 306brachypodium|gb169|BE425715 brachypodium 1370 157 maize|gb170|AI97492285.2 blastp 306 brachypodium|gb169|BE425715 brachypodium 1370 265maize|gb164|AI974922 81 blastp 307 maize|gb170|BG320615 maize 1371 157maize|gb170|AI974922 92.1 blastp 307 maize|gb170|BG320615 maize 1371 265maize|gb164|AI974922 86 blastp 308 maize|gb170|CF023721 maize 1372 157maize|gb170|AI974922 89.1 blastp 308 maize|gb170|CF023721 maize 1372 265maize|gb164|AI974922 87.5 blastp 309 maize|gb170|CF059393 maize 1373 157maize|gb170|AI974922 87.6 blastp 309 maize|gb170|CF059393 maize 1373 265maize|gb164|AI974922 86 blastp 310 maize|gb170|SRR014551S0286097 maize1374 265 maize|gb164|AI974922 88.1 blastp 310maize|gb170|SRR014551S0286097 maize 1374 157 maize|gb170|AI974922 85.1blastp 311 rice|gb170|OS11G09020 rice 1375 265 maize|gb164|AI97492283.56 tblastn 311 rice|gb170|OS11G09020 rice 1375 157maize|gb170|AI974922 80.1 blastp 312 rice|gb170|OS12G08090 rice 1376 157maize|gb170|AI974922 86 blastp 312 rice|gb170|OS12G08090 rice 1376 265maize|gb164|AI974922 81.3 blastp 313 rice|gb170|OS12G08130 rice 1377 157maize|gb170|AI974922 86.2 blastp 313 rice|gb170|OS12G08130 rice 1377 265maize|gb164|AI974922 81.5 blastp 314 sorghum|gb161.crp|BE358811 sorghum1378 157 maize|gb170|AI974922 95.6 blastp 314 sorghum|gb161.crp|BE358811sorghum 1378 265 maize|gb164|AI974922 89.8 blastp 315sorghum|gb161.crp|BG052599 sorghum 1379 157 maize|gb170|AI974922 89.1blastp 315 sorghum|gb161.crp|BG052599 sorghum 1379 265maize|gb164|AI974922 87.5 blastp 316 sorghum|gb161.crp|BG464355 sorghum1380 157 maize|gb170|AI974922 91 blastp 316 sorghum|gb161.crp|BG464355sorghum 1380 265 maize|gb164|AI974922 85.6 blastp 317sorghum|gb161.crp|BG488442 sorghum 1381 157 maize|gb170|AI974922 89.1blastp 317 sorghum|gb161.crp|BG488442 sorghum 1381 265maize|gb164|AI974922 87.7 blastp 318 sorghum|gb161.crp|SBGWP027891sorghum 1382 157 maize|gb170|AI974922 87.6 blastp 318sorghum|gb161.crp|SBGWP027891 sorghum 1382 265 maize|gb164|AI974922 86blastp 319 wheat|gb164|BI479031 wheat 1383 265 maize|gb164|AI97492281.74 tblastn 319 wheat|gb164|BI479031 wheat 1383 157maize|gb170|AI974922 80.33 tblastn 320 b_rapa|gb162|BG544047 b_rapa 1384158 arabidopsis|gb165|AT4G24960 87.5 blastp 321 b_rapa|gb162|EX087649b_rapa 1385 158 arabidopsis|gb165|AT4G24960 82.2 blastp 322canola|gb161|DY020042 canola 1386 158 arabidopsis|gb165|AT4G24960 86.8blastp 323 radish|gb164|EV538867 radish 1387 158arabidopsis|gb165|AT4G24960 84.4 blastp 324 radish|gb164|EV544902 radish1388 158 arabidopsis|gb165|AT4G24960 85.1 blastp 325radish|gb164|EX746928 radish 1389 158 arabidopsis|gb165|AT4G24960 84.4blastp 326 radish|gb164|EX748244 radish 1390 158arabidopsis|gb165|AT4G24960 83.9 blastp 327 thellungiella|gb167|BY812778thellungiella 1391 158 arabidopsis|gb165|AT4G24960 84.3 blastp 328apple|gb157.3|CN876940 apple 1392 159 tomato|gb164|BG124666 81.7 blastp329 apple|gb157.3|CN944710 apple 1393 159 tomato|gb164|BG124666 81.7blastp 330 apricot|gb157.2|CB819340 apricot 1394 159tomato|gb164|BG124666 82.3 blastp 331 b_oleracea|gb161|AM057864b_oleracea 1395 159 tomato|gb164|BG124666 80.6 blastp 332b_rapa|gb162|EE527690 b_rapa 1396 159 tomato|gb164|BG124666 80.6 blastp333 cacao|gb167|CU493876 cacao 1397 159 tomato|gb164|BG124666 80.3blastp 334 canola|gb161|CD830518 canola 1398 159 tomato|gb164|BG12466680 blastp 335 canola|gb161|CX279110 canola 1399 159tomato|gb164|BG124666 80.6 blastp 336 cassava|gb164|DV454217 cassava1400 159 tomato|gb164|BG124666 82.3 blastp 337catharanthus|gb166|EG557732 catharanthus 1401 159 tomato|gb164|BG12466681.1 blastp 338 citrus|gb166|CB290240 citrus 1402 159tomato|gb164|BG124666 83.4 blastp 339 coffea|gb157.2|DV694449 coffea1403 159 tomato|gb164|BG124666 83.4 blastp 340 cotton|gb164|AI727100cotton 1404 159 tomato|gb164|BG124666 83.7 blastp 341cynara|gb167|GE589728 cynara 1405 159 tomato|gb164|BG124666 80 blastp342 ipomoea|gb157.2|EE875432 ipomoea 1406 159 tomato|gb164|BG124666 81.7blastp 343 kiwi|gb166|FG405906 kiwi 1407 159 tomato|gb164|BG124666 81.6blastp 344 peach|gb157.2|BU044342 peach 1408 159 tomato|gb164|BG12466684 blastp 345 pepper|gb157.2|CA514905 pepper 1409 159tomato|gb164|BG124666 93.1 blastp 346 periwinkle|gb164|EG557732periwinkle 1410 159 tomato|gb164|BG124666 81.1 blastp 347petunia|gb166|CV294973 petunia 1411 159 tomato|gb164|BG124666 88.7tblastn 348 poplar|gb170|BU867493 poplar 1412 159 tomato|gb164|BG12466685.2 blastp 349 prunus|gb167|BU044342 prunus 1413 159tomato|gb164|BG124666 84 blastp 350 safflower|gb162|EL399778 safflower1414 159 tomato|gb164|BG124666 81.71 tblastn 351 soybean|gb168|AL371264soybean 1415 159 tomato|gb164|BG124666 81.1 blastp 352soybean|gb168|BE661867 soybean 1416 159 tomato|gb164|BG124666 80.6blastp 353 spurge|gb161|DV121886 spurge 1417 159 tomato|gb164|BG12466680.6 blastp 354 strawberry|gb164|DY670203 strawberry 1418 159tomato|gb164|BG124666 82.4 blastp 355 sunflower|gb162|EL460579 sunflower1419 159 tomato|gb164|BG124666 80 tblastn 356thellungiella|gb167|DN773683 thellungiella 1420 159tomato|gb164|BG124666 80.6 blastp 357 tobacco|gb162|EB445785 tobacco1421 159 tomato|gb164|BG124666 90.9 blastp 358 potato|gb157.2|BG098579potato 1422 160 tomato|gb164|BG134403 97.1 blastp 359potato|gb157.2|CK246251 potato 1423 160 tomato|gb164|BG134403 96.7blastp 360 potato|gb157.2|CK246610 potato 1424 160 tomato|gb164|BG13440396 blastp 361 brachypodium|gb169|BF260689 brachypodium 1425 162rice|gb157.2|AU174544 88.6 tblastn 362 maize|gb170|AI676864 maize 1426162 rice|gb157.2|AU174544 86.9 blastp 363 rice|gb170|OS02G44980 rice1427 162 rice|gb157.2|AU174544 80.6 blastp 364sorghum|gb161.crp|BF704932 sorghum 1428 162 rice|gb157.2|AU174544 89blastp 365 rice|gb170|OS12G38010 rice 1429 168 rice|gb157.2|BE03922191.14 tblastn 366 rice|gb170|OS12G38270 rice 1430 168rice|gb157.2|BE039221 81.2 blastp 367 rice|gb170|OS10G38040 rice 1431169 rice|gb157.2|AU056888 98.1 blastp 368 maize|gb170|BQ528487 maize1432 170 rice|gb157.2|AA753730 89.1 blastp 369sorghum|gb161.crp|CD221960 sorghum 1433 170 rice|gb157.2|AA753730 87.1blastp 370 switchgrass|gb167|DN149767 switchgrass 1434 170rice|gb157.2|AA753730 86.7 blastp 371 sorghum|gb161.crp|SBGWP095487sorghum 1435 171 maize|gb164|AW455682 89.3 blastp 372sugarcane|gb157.3|CA172410 sugarcane 1436 171 maize|gb164|AW455682 89blastp 373 switchgrass|gb167|DN144560 switchgrass 1437 171maize|gb164|AW455682 85.2 blastp 374 brachypodium|gb169|BE404970brachypodium 1438 172 rice|gb157.2|AA749861 88.7 blastp 375cenchrus|gb166|EB654111 cenchrus 1439 172 rice|gb157.2|AA749861 87blastp 376 leymus|gb166|EG400906 leymus 1440 172 rice|gb157.2|AA74986183.6 blastp 377 maize|gb170|AW018173 maize 1441 172rice|gb157.2|AA749861 90 blastp 378 maize|gb170|LLAI637139 maize 1442172 rice|gb157.2|AA749861 88.9 blastp 379 sorghum|gb161.crp|AI783434sorghum 1443 172 rice|gb157.2|AA749861 90.5 blastp 380sugarcane|gb157.3|BU925706 sugarcane 1444 172 rice|gb157.2|AA749861 91.2blastp 381 switchgrass|gb167|DN142209 switchgrass 1445 172rice|gb157.2|AA749861 91 blastp 382 switchgrass|gb167|DN142636switchgrass 1446 172 rice|gb157.2|AA749861 90.7 blastp 383wheat|gb164|BE398863 wheat 1447 172 rice|gb157.2|AA749861 80.9 blastp384 wheat|gb164|BE404970 wheat 1448 172 rice|gb157.2|AA749861 81.7blastp 385 wheat|gb164|BE418290 wheat 1449 172 rice|gb157.2|AA749861 82blastp 386 maize|gb170|BM895695 maize 1450 173 rice|gb157.2|AK10899484.5 blastp 387 rice|gb170|OS04G55740 rice 1451 173rice|gb157.2|AK108994 94.7 blastp 388 sorghum|gb161.crp|BM895695 sorghum1452 173 rice|gb157.2|AK108994 82.2 blastp 389brachypodium|gb169|CA684980 brachypodium 1453 174 rice|gb157.2|CB64073287.2 blastp 390 maize|gb170|AW562805 maize 1454 174rice|gb157.2|CB640732 87.1 blastp 391 sorghum|gb161.crp|CD219694 sorghum1455 174 rice|gb157.2|CB640732 87.7 blastp 392 soybean|gb168|AL366192soybean 1456 174 rice|gb157.2|CB640732 80.38 tblastn 393poplar|gb170|AI166596 poplar 1457 175 poplar|gb157.2|BU868634 88.2blastp 394 castorbean|gb160|AJ605572 castorbean 1458 176soybean|gb162|CA852963 81 blastp 395 chestnut|gb170|SRR006296S0014660chestnut 1459 176 soybean|gb162|CA852963 80.08 tblastn 396citrus|gb166|CK740163 citrus 1460 176 soybean|gb162|CA852963 80.08tblastn 397 cowpea|gb166|FF394551 cowpea 1461 176 soybean|gb162|CA85296390.7 blastp 398 medicago|gb157.2|AA660751 medicago 1462 176soybean|gb162|CA852963 87.9 blastp 399 peanut|gb167|EH042453 peanut 1463176 soybean|gb162|CA852963 88.66 tblastn 400 soybean|gb168|BU547671soybean 1464 176 soybean|gb162|CA852963 97.2 blastp 401barley|gb157.3|BE194421 barley 1465 177 rice|gb157.2|BI811377 81.5blastp 402 brachypodium|gb169|BE424330 brachypodium 1466 177rice|gb157.2|BI811377 82.4 blastp 403 leymus|gb166|EG376396 leymus 1467177 rice|gb157.2|BI811377 81.8 blastp 404 pseudoroegneria|gb167|FF349876pseudoroegneria 1468 177 rice|gb157.2|BI811377 82.1 blastp 405sugarcane|gb157.3|CA099115 sugarcane 1469 177 rice|gb157.2|BI811377 81blastp 406 wheat|gb164|BE424330 wheat 1470 177 rice|gb157.2|BI81137781.82 tblastn 407 wheat|gb164|BE516775 wheat 1471 177rice|gb157.2|BI811377 82.1 blastp 408 antirrhinum|gb166|AJ560033antirrhinum 1472 180 tomato|gb164|BG129806 82.9 blastp 409antirrhinum|gb166|AJ801252 antirrhinum 1473 180 tomato|gb164|BG12980683.3 blastp 410 apple|gb157.3|AU301287 apple 1474 180tomato|gb164|BG129806 86.9 blastp 411 apple|gb157.3|CN488989 apple 1475180 tomato|gb164|BG129806 84.7 blastp 412 apple|gb157.3|CN864173 apple1476 180 tomato|gb164|BG129806 84.7 blastp 413 apple|gb157.3|CN869339apple 1477 180 tomato|gb164|BG129806 87.4 blastp 414aquilegia|gb157.3|DR939874 aquilegia 1478 180 tomato|gb164|BG129806 81.5blastp 415 arabidopsis|gb165|AT1G04750 arabidopsis 1479 180tomato|gb164|BG129806 81.5 blastp 416 arabidopsis|gb165|AT2G33120arabidopsis 1480 180 tomato|gb164|BG129806 82 blastp 417artemisia|gb164|EY060063 artemisia 1481 180 tomato|gb164|BG129806 84.7blastp 418 artemisia|gb164|EY073689 artemisia 1482 180tomato|gb164|BG129806 84.7 blastp 419 avocado|gb164|CK762777 avocado1483 180 tomato|gb164|BG129806 82.9 blastp 420 avocado|gb164|CV461025avocado 1484 180 tomato|gb164|BG129806 80.6 blastp 421b_oleracea|gb161|AM386735 b_oleracea 1485 180 tomato|gb164|BG129806 80.6blastp 422 b_oleracea|gb161|CB617574 b_oleracea 1486 180tomato|gb164|BG129806 81.6 blastp 423 b_rapa|gb162|CA992099 b_rapa 1487180 tomato|gb164|BG129806 80.2 blastp 424 b_rapa|gb162|CV544695 b_rapa1488 180 tomato|gb164|BG129806 82.4 blastp 425 b_rapa|gb162|DN961220b_rapa 1489 180 tomato|gb164|BG129806 82.1 blastp 426b_rapa|gb162|DY008890 b_rapa 1490 180 tomato|gb164|BG129806 82.4 blastp427 b_rapa|gb162|EX034829 b_rapa 1491 180 tomato|gb164|BG129806 80.6blastp 428 banana|gb167|FF558354 banana 1492 180 tomato|gb164|BG12980680.4 blastp 429 banana|gb167|FL658702 banana 1493 180tomato|gb164|BG129806 82 blastp 430 barley|gb157.3|BE413339 barley 1494180 tomato|gb164|BG129806 80.8 blastp 431 basilicum|gb157.3|DY343103basilicum 1495 180 tomato|gb164|BG129806 84.1 blastp 432bean|gb167|CA898578 bean 1496 180 tomato|gb164|BG129806 83.8 blastp 433bean|gb167|CA899486 bean 1497 180 tomato|gb164|BG129806 87.2 blastp 434bean|gb167|CA907867 bean 1498 180 tomato|gb164|BG129806 87.4 blastp 435brachypodium|gb169|BE417694 brachypodium 1499 180 tomato|gb164|BG12980680.2 blastp 436 cacao|gb167|CA798042 cacao 1500 180tomato|gb164|BG129806 83.3 blastp 437 cacao|gb167|CU474349 cacao 1501180 tomato|gb164|BG129806 90.5 blastp 438 cacao|gb167|CU478046 cacao1502 180 tomato|gb164|BG129806 85.1 blastp 439 canola|gb161|CD816574canola 1503 180 tomato|gb164|BG129806 82.4 blastp 440canola|gb161|CD818619 canola 1504 180 tomato|gb164|BG129806 80.2 blastp441 canola|gb161|CD826636 canola 1505 180 tomato|gb164|BG129806 82.9blastp 442 canola|gb161|CD841484 canola 1506 180 tomato|gb164|BG12980682.4 blastp 443 canola|gb161|CN734885 canola 1507 180tomato|gb164|BG129806 81.5 blastp 444 canola|gb161|DW998530 canola 1508180 tomato|gb164|BG129806 82.1 blastp 445 canola|gb161|DY028580 canola1509 180 tomato|gb164|BG129806 82.5 blastp 446 canola|gb161|EE483345canola 1510 180 tomato|gb164|BG129806 80.2 blastp 447cassava|gb164|BM259789 cassava 1511 180 tomato|gb164|BG129806 84.2blastp 448 cassava|gb164|CK645968 cassava 1512 180 tomato|gb164|BG12980684.7 blastp 449 cassava|gb164|DV446794 cassava 1513 180tomato|gb164|BG129806 82.4 blastp 450 castorbean|gb160|EE255473castorbean 1514 180 tomato|gb164|BG129806 82.9 blastp 451castorbean|gb160|EE255572 castorbean 1515 180 tomato|gb164|BG129806 85.1blastp 452 castorbean|gb160|EE259993 castorbean 1516 180tomato|gb164|BG129806 86 blastp 453 centaurea|gb166|EH728993 centaurea1517 180 tomato|gb164|BG129806 84.3 blastp 454 centaurea|gb166|EH737653centaurea 1518 180 tomato|gb164|BG129806 83.33 tblastn 455centaurea|gb166|EH743515 centaurea 1519 180 tomato|gb164|BG129806 84.7blastp 456 centaurea|gb166|EH747496 centaurea 1520 180tomato|gb164|BG129806 82 blastp 457 chestnut|gb170|SRR006295S0000799chestnut 1521 180 tomato|gb164|BG129806 84.7 blastp 458chestnut|gb170|SRR006295S0000895 chestnut 1522 180 tomato|gb164|BG12980685.7 blastp 459 cichorium|gb166|DT212405 cichorium 1523 180tomato|gb164|BG129806 83.33 tblastn 460 cichorium|gb166|DT212482cichorium 1524 180 tomato|gb164|BG129806 84.7 blastp 461cichorium|gb166|EH686887 cichorium 1525 180 tomato|gb164|BG129806 82.88tblastn 462 citrus|gb166|BE205677 citrus 1526 180 tomato|gb164|BG12980688.3 blastp 463 citrus|gb166|CB290704 citrus 1527 180tomato|gb164|BG129806 83.3 blastp 464 citrus|gb166|CF830698 citrus 1528180 tomato|gb164|BG129806 83.8 blastp 465 coffea|gb157.2|CF588660 coffea1529 180 tomato|gb164|BG129806 82.9 blastp 466 coffea|gb157.2|DV665256coffea 1530 180 tomato|gb164|BG129806 80.5 blastp 467cotton|gb164|AI055143 cotton 1531 180 tomato|gb164|BG129806 82.4 blastp468 cotton|gb164|AI726538 cotton 1532 180 tomato|gb164|BG129806 82.43tblastn 469 cotton|gb164|BF268281 cotton 1533 180 tomato|gb164|BG12980688.3 blastp 470 cotton|gb164|BF270800 cotton 1534 180tomato|gb164|BG129806 85.1 blastp 471 cotton|gb164|BF274309 cotton 1535180 tomato|gb164|BG129806 88.8 blastp 472 cowpea|gb166|FF382703 cowpea1536 180 tomato|gb164|BG129806 84.2 blastp 473 cowpea|gb166|FF385500cowpea 1537 180 tomato|gb164|BG129806 87.4 blastp 474cowpea|gb166|FF388694 cowpea 1538 180 tomato|gb164|BG129806 88 blastp475 cycas|gb166|CB090084 cycas 1539 180 tomato|gb164|BG129806 80.6blastp 476 cynara|gb167|GE583641 cynara 1540 180 tomato|gb164|BG12980681.98 tblastn 477 cynara|gb167|GE586008 cynara 1541 180tomato|gb164|BG129806 80.18 tblastn 478 dandelion|gb161|DY820375dandelion 1542 180 tomato|gb164|BG129806 84.23 tblastn 479dandelion|gb161|DY822153 dandelion 1543 180 tomato|gb164|BG129806 85.1blastp 480 fescue|gb161|DT686644 fescue 1544 180 tomato|gb164|BG12980682.9 blastp 481 ginger|gb164|DY354490 ginger 1545 180tomato|gb164|BG129806 82.9 blastp 482 ginger|gb164|DY357009 ginger 1546180 tomato|gb164|BG129806 81.53 tblastn 483 grape|gb160|BQ797249 grape1547 180 tomato|gb164|BG129806 84.2 blastp 484 grape|gb160|CA814878grape 1548 180 tomato|gb164|BG129806 83.4 blastp 485grape|gb160|CB009359 grape 1549 180 tomato|gb164|BG129806 83.8 blastp486 ipomoea|gb157.2|BJ554498 ipomoea 1550 180 tomato|gb164|BG129806 90.1blastp 487 ipomoea|gb157.2|BJ555833 ipomoea 1551 180tomato|gb164|BG129806 89.6 blastp 488 ipomoea|gb157.2|BJ565525 ipomoea1552 180 tomato|gb164|BG129806 89.6 blastp 489 ipomoea|gb157.2|DQ016990ipomoea 1553 180 tomato|gb164|BG129806 82 blastp 490 kiwi|gb166|FG428824kiwi 1554 180 tomato|gb164|BG129806 81.5 blastp 491lettuce|gb157.2|DW046480 lettuce 1555 180 tomato|gb164|BG129806 85.1blastp 492 lettuce|gb157.2|DW051770 lettuce 1556 180tomato|gb164|BG129806 80.6 blastp 493 lettuce|gb157.2|DW054433 lettuce1557 180 tomato|gb164|BG129806 84.7 blastp 494 lettuce|gb157.2|DW104005lettuce 1558 180 tomato|gb164|BG129806 83.8 blastp 495lettuce|gb157.2|DW148893 lettuce 1559 180 tomato|gb164|BG129806 84.7blastp 496 liriodendron|gb166|CK761427 liriodendron 1560 180tomato|gb164|BG129806 81.1 blastp 497 lovegrass|gb167|EH189433 lovegrass1561 180 tomato|gb164|BG129806 81.5 blastp 498 maize|gb170|AI621444maize 1562 180 tomato|gb164|BG129806 83 blastp 499 maize|gb170|AI901672maize 1563 180 tomato|gb164|BG129806 81.5 blastp 500medicago|gb157.2|AL371369 medicago 1564 180 tomato|gb164|BG129806 81.3blastp 501 medicago|gb157.2|AW127543 medicago 1565 180tomato|gb164|BG129806 85.3 blastp 502 medicago|gb157.2|AW329342 medicago1566 180 tomato|gb164|BG129806 81.5 blastp 503 melon|gb165|AM743036melon 1567 180 tomato|gb164|BG129806 82.9 blastp 504melon|gb165|DV633620 melon 1568 180 tomato|gb164|BG129806 80.6 blastp505 nuphar|gb166|ES730054 nuphar 1569 180 tomato|gb164|BG129806 81.2blastp 506 oak|gb170|CU639508 oak 1570 180 tomato|gb164|BG129806 85.7blastp 507 oak|gb170|SRR006307S0008904 oak 1571 180tomato|gb164|BG129806 84.7 blastp 508 oil_palm|gb166|CN599846 oil_palm1572 180 tomato|gb164|BG129806 82 blastp 509 onion|gb162|CF440003 onion1573 180 tomato|gb164|BG129806 82.43 tblastn 510 papaya|gb165|AM904122papaya 1574 180 tomato|gb164|BG129806 84.2 blastp 511papaya|gb165|EX245134 papaya 1575 180 tomato|gb164|BG129806 83.8 blastp512 peach|gb157.2|BU040787 peach 1576 180 tomato|gb164|BG129806 88.7blastp 513 peach|gb157.2|BU048627 peach 1577 180 tomato|gb164|BG12980681.53 tblastn 514 peanut|gb167|EH042957 peanut 1578 180tomato|gb164|BG129806 88.4 blastp 515 peanut|gb167|EH044861 peanut 1579180 tomato|gb164|BG129806 83 blastp 516 pepper|gb157.2|CA520584 pepper1580 180 tomato|gb164|BG129806 82.4 blastp 517 petunia|gb166|CV296853petunia 1581 180 tomato|gb164|BG129806 82.9 blastp 518pineapple|gb157.2|DT337519 pineapple 1582 180 tomato|gb164|BG129806 83.3blastp 519 poplar|gb170|AI166018 poplar 1583 180 tomato|gb164|BG12980686.9 blastp 520 poplar|gb170|BI120322 poplar 1584 180tomato|gb164|BG129806 82.9 blastp 521 poplar|gb170|BI128184 poplar 1585180 tomato|gb164|BG129806 81.1 blastp 522 poplar|gb170|BU818354 poplar1586 180 tomato|gb164|BG129806 87.8 blastp 523 poplar|gb170|CB240411poplar 1587 180 tomato|gb164|BG129806 81.1 blastp 524potato|gb157.2|BG590329 potato 1588 180 tomato|gb164|BG129806 80.3blastp 525 potato|gb157.2|BG886984 potato 1589 180 tomato|gb164|BG12980682.9 blastp 526 potato|gb157.2|BI406651 potato 1590 180tomato|gb164|BG129806 100 blastp 527 prunus|gb167|BU040787 prunus 1591180 tomato|gb164|BG129806 88.7 blastp 528 prunus|gb167|BU048627 prunus1592 180 tomato|gb164|BG129806 85.6 blastp 529pseudoroegneria|gb167|FF341379 pseudoroegneria 1593 180tomato|gb164|BG129806 81.2 blastp 530 radish|gb164|EV527352 radish 1594180 tomato|gb164|BG129806 82.5 blastp 531 radish|gb164|EV528724 radish1595 180 tomato|gb164|BG129806 82 blastp 532 radish|gb164|EV532638radish 1596 180 tomato|gb164|BG129806 82 blastp 533radish|gb164|EV535212 radish 1597 180 tomato|gb164|BG129806 82.5 blastp534 radish|gb164|EV544241 radish 1598 180 tomato|gb164|BG129806 82blastp 535 radish|gb164|EV549527 radish 1599 180 tomato|gb164|BG12980681.98 tblastn 536 radish|gb164|EV567707 radish 1600 180tomato|gb164|BG129806 82 blastp 537 radish|gb164|EW724564 radish 1601180 tomato|gb164|BG129806 82 blastp 538 radish|gb164|EX755021 radish1602 180 tomato|gb164|BG129806 80.7 blastp 539 rice|gb170|OS03G58840rice 1603 180 tomato|gb164|BG129806 82.9 blastp 540rice|gb170|OS07G09600 rice 1604 180 tomato|gb164|BG129806 82.9 blastp541 safflower|gb162|EL373980 safflower 1605 180 tomato|gb164|BG12980684.7 blastp 542 safflower|gb162|EL381462 safflower 1606 180tomato|gb164|BG129806 83.8 blastp 543 safflower|gb162|EL389501 safflower1607 180 tomato|gb164|BG129806 81.5 blastp 544 safflower|gb162|EL404279safflower 1608 180 tomato|gb164|BG129806 84.7 blastp 545senecio|gb170|DY663178 senecio 1609 180 tomato|gb164|BG129806 81.08tblastn 546 sorghum|gb161.crp|AW282206 sorghum 1610 180tomato|gb164|BG129806 81.5 blastp 547 sorghum|gb161.crp|AW565015 sorghum1611 180 tomato|gb164|BG129806 83 blastp 548 soybean|gb168|AL371369soybean 1612 180 tomato|gb164|BG129806 87.8 blastp 549soybean|gb168|AL371370 soybean 1613 180 tomato|gb164|BG129806 87.6blastp 550 soybean|gb168|AL374552 soybean 1614 180 tomato|gb164|BG12980687.4 blastp 551 soybean|gb168|AL384290 soybean 1615 180tomato|gb164|BG129806 86.7 blastp 552 soybean|gb168|BE658783 soybean1616 180 tomato|gb164|BG129806 84.7 blastp 553 soybean|gb168|BE660085soybean 1617 180 tomato|gb164|BG129806 85.1 blastp 554spurge|gb161|DV113185 spurge 1618 180 tomato|gb164|BG129806 83.8 blastp555 spurge|gb161|DV115533 spurge 1619 180 tomato|gb164|BG129806 86.5blastp 556 spurge|gb161|DV129528 spurge 1620 180 tomato|gb164|BG12980682.4 blastp 557 strawberry|gb164|CO380944 strawberry 1621 180tomato|gb164|BG129806 87.4 blastp 558 strawberry|gb164|DY667942strawberry 1622 180 tomato|gb164|BG129806 83.8 blastp 559sugarcane|gb157.3|CA066679 sugarcane 1623 180 tomato|gb164|BG12980681.53 tblastn 560 sugarcane|gb157.3|CA070863 sugarcane 1624 180tomato|gb164|BG129806 83 blastp 561 sugarcane|gb157.3|CA073069 sugarcane1625 180 tomato|gb164|BG129806 82.5 blastp 562sugarcane|gb157.3|CA098212 sugarcane 1626 180 tomato|gb164|BG129806 81.1blastp 563 sugarcane|gb157.3|CA105955 sugarcane 1627 180tomato|gb164|BG129806 83 blastp 564 sugarcane|gb157.3|CA125341 sugarcane1628 180 tomato|gb164|BG129806 83 blastp 565 sunflower|gb162|CD848438sunflower 1629 180 tomato|gb164|BG129806 83.8 blastp 566sunflower|gb162|CD855829 sunflower 1630 180 tomato|gb164|BG129806 84.7blastp 567 sunflower|gb162|DY909391 sunflower 1631 180tomato|gb164|BG129806 84.7 blastp 568 sunflower|gb162|EL423569 sunflower1632 180 tomato|gb164|BG129806 83.3 blastp 569 sunflower|gb162|EL429220sunflower 1633 180 tomato|gb164|BG129806 85.1 blastp 570switchgrass|gb167|DN143573 switchgrass 1634 180 tomato|gb164|BG12980682.4 blastp 571 switchgrass|gb167|DN151435 switchgrass 1635 180tomato|gb164|BG129806 82.9 blastp 572 switchgrass|gb167|FE607763switchgrass 1636 180 tomato|gb164|BG129806 83 blastp 573switchgrass|gb167|FE624609 switchgrass 1637 180 tomato|gb164|BG12980684.2 blastp 574 thellungiella|gb167|BY802757 thellungiella 1638 180tomato|gb164|BG129806 81.5 blastp 575 tobacco|gb162|DV157924 tobacco1639 180 tomato|gb164|BG129806 82.4 blastp 576 tobacco|gb162|EB426444tobacco 1640 180 tomato|gb164|BG129806 96.4 tblastn 577tobacco|gb162|EB426574 tobacco 1641 180 tomato|gb164|BG129806 84.7blastp 578 tobacco|gb162|EB677916 tobacco 1642 180 tomato|gb164|BG12980694.1 blastp 579 tomato|gb164|BG135003 tomato 1643 180tomato|gb164|BG129806 84.2 blastp 580 tomato|gb164|BG629456 tomato 1644180 tomato|gb164|BG129806 82.9 blastp 581 triphysaria|gb164|DR172719triphysaria 1645 180 tomato|gb164|BG129806 82.3 blastp 582triphysaria|gb164|EY126667 triphysaria 1646 180 tomato|gb164|BG12980683.8 blastp 583 triphysaria|gb164|EY128979 triphysaria 1647 180tomato|gb164|BG129806 83.8 blastp 584 walnuts|gb166|CV198306 walnuts1648 180 tomato|gb164|BG129806 85.7 blastp 585 wheat|gb164|BE400499wheat 1649 180 tomato|gb164|BG129806 80.8 blastp 586wheat|gb164|BE417694 wheat 1650 180 tomato|gb164|BG129806 81.2 blastp587 wheat|gb164|CA595472 wheat 1651 180 tomato|gb164|BG129806 81.2blastp 588 zamia|gb166|FD768487 zamia 1652 180 tomato|gb164|BG12980680.2 blastp 589 barley|gb157.3|AL450674 barley 1653 183rice|gb157.2|AT003383 85.7 blastp 590 brachypodium|gb169|BE424284brachypodium 1654 183 rice|gb157.2|AT003383 83.4 blastp 591fescue|gb161|DT675288 fescue 1655 183 rice|gb157.2|AT003383 85.4 blastp592 leymus|gb166|CN466264 leymus 1656 183 rice|gb157.2|AT003383 84.6blastp 593 maize|gb170|AI438809 maize 1657 183 rice|gb157.2|AT00338384.4 blastp 594 maize|gb170|AI977870 maize 1658 183rice|gb157.2|AT003383 82.8 blastp 595 maize|gb170|LLDQ245361 maize 1659183 rice|gb157.2|AT003383 85.7 blastp 596 pseudoroegneria|gb167|FF341007pseudoroegneria 1660 183 rice|gb157.2|AT003383 85.7 blastp 597rye|gb164|BE586725 rye 1661 183 rice|gb157.2|AT003383 85.7 blastp 598sorghum|gb161.crp|AW565030 sorghum 1662 183 rice|gb157.2|AT003383 82.9blastp 599 sugarcane|gb157.3|CA084082 sugarcane 1663 183rice|gb157.2|AT003383 83.1 blastp 600 switchgrass|gb167|DN142592switchgrass 1664 183 rice|gb157.2|AT003383 86.4 blastp 601switchgrass|gb167|DN145453 switchgrass 1665 183 rice|gb157.2|AT003383 85blastp 602 wheat|gb164|BE424284 wheat 1666 183 rice|gb157.2|AT00338385.7 blastp 603 wheat|gb164|BE498139 wheat 1667 183rice|gb157.2|AT003383 85.3 blastp 604 wheat|gb164|BF200880 wheat 1668183 rice|gb157.2|AT003383 85.1 blastp 605 wheat|gb164|CA620728 wheat1669 183 rice|gb157.2|AT003383 81.67 tblastn 606brachypodium|gb169|AJ476542 brachypodium 1670 185 maize|gb157|BG35453591 blastp 607 leymus|gb166|EG388555 leymus 1671 185 maize|gb157|BG35453588.3 blastp 608 pseudoroegneria|gb167|FF346414 pseudoroegneria 1672 185maize|gb157|BG354535 88.9 blastp 609 rice|gb170|OS01G51190 rice 1673 185maize|gb157|BG354535 91.57 tblastn 610 sorghum|gb161.crp|AW283867sorghum 1674 185 maize|gb157|BG354535 98.8 blastp 611wheat|gb164|AL820971 wheat 1675 185 maize|gb157|BG354535 88.3 blastp 612maize|gb170|BI388811 maize 1676 186 rice|gb157.2|AU029933 82.8 blastp613 sorghum|gb161.crp|DR807282 sorghum 1677 186 rice|gb157.2|AU029933 82blastp 614 rice|gb170|OS01G65169 rice 1678 187 rice|gb157.2|AK10223982.5 blastp 615 brachypodium|gb169|BE421953 brachypodium 1679 188sorghum|gb161.xeno|AI947781 81.97 tblastn 616 maize|gb170|AI947781 maize1680 188 sorghum|gb161.xeno|AI947781 95.8 blastp 617rice|gb170|OS01G65100 rice 1681 188 sorghum|gb161.xeno|AI947781 87blastp 618 switchgrass|gb167|DN144961 switchgrass 1682 188sorghum|gb161.xeno|AI947781 90.2 tblastn 619 canola|gb161|EE417585canola 1683 189 arabidopsis|gb165|AT1G58030 89 blastp 620radish|gb164|EV566943 radish 1684 189 arabidopsis|gb165|AT1G58030 89.47tblastn 621 barley|gb157.3|BE412663 barley 1685 191 maize|gb164|AI60056384.2 blastp 622 brachypodium|gb169|BE407009 brachypodium 1686 191maize|gb164|AI600563 88.6 blastp 623 rice|gb170|OS04G56290 rice 1687 191maize|gb164|AI600563 88.1 blastp 624 sorghum|gb161.crp|AI622153 sorghum1688 191 maize|gb164|AI600563 95.3 blastp 625 sugarcane|gb157.3|CA067412sugarcane 1689 191 maize|gb164|AI600563 95 blastp 626switchgrass|gb167|DN150103 switchgrass 1690 191 maize|gb164|AI60056393.5 blastp 627 wheat|gb164|BE407009 wheat 1691 191 maize|gb164|AI60056387.5 blastp 628 rice|gb170|OS01G03530 rice 1692 192rice|gb157.2|CB000630 99.8 blastp 629 barley|gb157.3|BF066082 barley1693 193 wheat|gb154|TG_BE216912 88.03 tblastn 630barley|gb157.3|HVU08135 barley 1694 193 wheat|gb154|TG_BE216912 81.7blastp 631 brachypodium|gb169|HVU08135 brachypodium 1695 193wheat|gb154|TG_BE216912 91.1 blastp 632 fescue|gb161|DT682842 fescue1696 193 wheat|gb154|TG_BE216912 86.5 blastp 633 leymus|gb166|CD808858leymus 1697 193 wheat|gb154|TG_BE216912 97.4 blastp 634maize|gb170|AI491463 maize 1698 193 wheat|gb154|TG_BE216912 84.3 blastp635 maize|gb170|AI601031 maize 1699 193 wheat|gb154|TG_BE216912 84.9blastp 636 pseudoroegneria|gb167|FF347239 pseudoroegneria 1700 193wheat|gb154|TG_BE216912 98.3 blastp 637 rice|gb170|OS03G56670 rice 1701193 wheat|gb154|TG_BE216912 85 blastp 638 rye|gb164|BE636806 rye 1702193 wheat|gb154|TG_BE216912 84 blastp 639 sorghum|gb161.crp|AI861201sorghum 1703 193 wheat|gb154|TG_BE216912 85.4 blastp 640switchgrass|gb167|DN144671 switchgrass 1704 193 wheat|gb154|TG_BE21691284.4 blastp 641 wheat|gb164|BE213564 wheat 1705 193wheat|gb154|TG_BE216912 91.5 blastp 642 wheat|gb164|BE216912 wheat 1706193 wheat|gb154|TG_BE216912 89.8 blastp 643 wheat|gb164|BE415875 wheat1707 193 wheat|gb154|TG_BE216912 93.5 blastp 644 wheat|gb164|CK217408wheat 1708 193 wheat|gb154|TG_BE216912 83.12 tblastn 645wheat|gb164|DR737269 wheat 1709 193 wheat|gb154|TG_BE216912 82.25tblastn 646 leymus|gb166|EG400892 leymus 1710 194 rice|gb157.2|BE03921881.9 blastp 647 b_oleracea|gb161|AM059989 b_oleracea 1711 195arabidopsis|gb165|AT5G60680 80.6 blastp 648 canola|gb161|DW997913 canola1712 195 arabidopsis|gb165|AT5G60680 81.2 blastp 649 bean|gb167|CA898406bean 1713 196 rice|gb157.2|AA750934 80.5 blastp 650 cacao|gb167|CU469591cacao 1714 196 rice|gb157.2|AA750934 80.6 blastp 651cassava|gb164|CK641441 cassava 1715 196 rice|gb157.2|AA750934 80.9blastp 652 castorbean|gb160|T15009 castorbean 1716 196rice|gb157.2|AA750934 80.2 blastp 653 cowpea|gb166|FC457559 cowpea 1717196 rice|gb157.2|AA750934 80.2 blastp 654 cowpea|gb166|FC461906 cowpea1718 196 rice|gb157.2|AA750934 80.3 blastp 655 maize|gb170|W21620 maize1719 196 rice|gb157.2|AA750934 87.6 blastp 656 oil_palm|gb166|CN601354oil_palm 1720 196 rice|gb157.2|AA750934 81.1 blastp 657papaya|gb165|EX264224 papaya 1721 196 rice|gb157.2|AA750934 80 blastp658 pineapple|gb157.2|CO730751 pineapple 1722 196 rice|gb157.2|AA75093481.6 blastp 659 sorghum|gb161.crp|W21620 sorghum 1723 196rice|gb157.2|AA750934 87.4 blastp 660 soybean|gb168|AL373484 soybean1724 196 rice|gb157.2|AA750934 80.9 blastp 661 soybean|gb168|AW348141soybean 1725 196 rice|gb157.2|AA750934 80.4 blastp 662soybean|gb168|AW587090 soybean 1726 196 rice|gb157.2|AA750934 80.4blastp 663 sugarcane|gb157.3|BQ535675 sugarcane 1727 196rice|gb157.2|AA750934 86.8 blastp 664 switchgrass|gb167|DN140694switchgrass 1728 196 rice|gb157.2|AA750934 86.1 blastp 665switchgrass|gb167|DN141888 switchgrass 1729 196 rice|gb157.2|AA75093485.8 blastp 666 switchgrass|gb167|FE603746 switchgrass 1730 196rice|gb157.2|AA750934 85.3 blastp 667 maize|gb170|BE129570 maize 1731198 maize|gb154|AW037179 96.1 blastp 668 maize|gb170|BI478834 maize 1732198 maize|gb154|AW037179 92.9 blastp 669 rice|gb170|OS02G54730 rice 1733198 maize|gb154|AW037179 81.4 blastp 670 sorghum|gb161.crp|BE129570sorghum 1734 198 maize|gb154|AW037179 91.8 blastp 671sorghum|gb161.crp|CD231473 sorghum 1735 199 maize|gb164|AW287760 81.98tblastn 672 sugarcane|gb157.3|CA089926 sugarcane 1736 199maize|gb164|AW287760 88.56 tblastn 673 switchgrass|gb167|FL699406switchgrass 1737 199 maize|gb164|AW287760 85.3 blastp 674switchgrass|gb167|FL727557 switchgrass 1738 199 maize|gb164|AW28776081.9 blastp 675 sorghum|gb161.crp|BF480947 sorghum 1739 200maize|gb157|AW360667 96.1 blastp 676 sugarcane|gb157.3|CA069365sugarcane 1740 200 maize|gb157|AW360667 97.2 blastp 677apple|gb157.3|CN873722 apple 1741 202 arabidopsis|gb157.2|AT3G2610080.26 tblastn 678 chestnut|gb170|SRR006295S0001486 chestnut 1742 202arabidopsis|gb157.2|AT3G26100 82.2 blastp 679 cotton|gb164|AI728964cotton 1743 202 arabidopsis|gb157.2|AT3G26100 80.3 blastp 680grape|gb160|CB004507 grape 1744 202 arabidopsis|gb157.2|AT3G26100 80.3blastp 681 papaya|gb165|EX243430 papaya 1745 202arabidopsis|gb157.2|AT3G26100 82.8 blastp 682 poplar|gb170|AI165788poplar 1746 202 arabidopsis|gb157.2|AT3G26100 80.26 tblastn 683poplar|gb170|BI071804 poplar 1747 202 arabidopsis|gb157.2|AT3G2610080.75 tblastn 684 bean|gb167|CV538336 bean 1748 203 soybean|gb162|SOYHPR80.83 tblastn 685 bean|gb167|PVU72768 bean 1749 203 soybean|gb162|SOYHPR85.8 blastp 686 clover|gb162|BB932705 clover 1750 203soybean|gb162|SOYHPR 83.3 blastp 687 cowpea|gb166|FC457443 cowpea 1751203 soybean|gb162|SOYHPR 87.92 tblastn 688 medicago|gb157.2|AL366760medicago 1752 203 soybean|gb162|SOYHPR 84.7 blastp 689medicago|gb157.2|AW328889 medicago 1753 203 soybean|gb162|SOYHPR 80.52tblastn 690 medicago|gb157.2|AW329415 medicago 1754 203soybean|gb162|SOYHPR 83.67 tblastn 691 medicago|gb157.2|AW329734medicago 1755 203 soybean|gb162|SOYHPR 85.11 tblastn 692soybean|gb168|S44202 soybean 1756 203 soybean|gb162|SOYHPR 95.7 blastp693 b_rapa|gb162|CV544929 b_rapa 1757 266 arabidopsis|gb157.2|AT1G4492080.38 tblastn 693 b_rapa|gb162|CV544929 b_rapa 1757 204arabidopsis|gb165|AT1G44920 80.15 tblastn 694 radish|gb164|EV525414radish 1758 266 arabidopsis|gb157.2|AT1G44920 81.3 blastp 694radish|gb164|EV525414 radish 1758 204 arabidopsis|gb165|AT1G44920 80.7blastp 695 thellungiella|gb167|DN777579 thellungiella 1759 266arabidopsis|gb157.2|AT1G44920 81.7 blastp 695thellungiella|gb167|DN777579 thellungiella 1759 204arabidopsis|gb165|AT1G44920 81.1 blastp 696 arabidopsis|gb165|AT3G17410arabidopsis 1760 205 arabidopsis|gb157.2|AT1G48210 87.1 blastp 697b_oleracea|gb161|AM385784 b_oleracea 1761 205arabidopsis|gb157.2|AT1G48210 87.4 blastp 698 b_rapa|gb162|DN962030b_rapa 1762 205 arabidopsis|gb157.2|AT1G48210 83.52 tblastn 699b_rapa|gb162|EX020680 b_rapa 1763 205 arabidopsis|gb157.2|AT1G48210 85.4blastp 700 b_rapa|gb162|EX025892 b_rapa 1764 205arabidopsis|gb157.2|AT1G48210 85.99 tblastn 701 canola|gb161|CX278279canola 1765 205 arabidopsis|gb157.2|AT1G48210 86.3 blastp 702canola|gb161|EG021170 canola 1766 205 arabidopsis|gb157.2|AT1G4821083.79 tblastn 703 radish|gb164|EV525080 radish 1767 205arabidopsis|gb157.2|AT1G48210 87.6 blastp 704 radish|gb164|EV543636radish 1768 205 arabidopsis|gb157.2|AT1G48210 85.4 blastp 705radish|gb164|EY895533 radish 1769 205 arabidopsis|gb157.2|AT1G48210 84.7blastp 706 thellungiella|gb167|DN774052 thellungiella 1770 205arabidopsis|gb157.2|AT1G48210 87.4 blastp 707 wheat|gb164|AL822688 wheat1771 206 wheat|gb164|BE445396 92.9 blastp 708 wheat|gb164|CD869154 wheat1772 206 wheat|gb164|BE445396 92.9 blastp 709 banana|gb167|ES432415banana 1773 208 rice|gb157.2|AU077950 85.3 blastp 710barley|gb157.3|BI948762 barley 1774 208 rice|gb157.2|AU077950 81.5blastp 711 barley|gb157.3|BJ453298 barley 1775 208 rice|gb157.2|AU07795094.2 blastp 712 citrus|gb166|CF419725 citrus 1776 208rice|gb157.2|AU077950 80.8 blastp 713 cotton|gb164|BF277609 cotton 1777208 rice|gb157.2|AU077950 81.5 blastp 714 fescue|gb161|CK801460 fescue1778 208 rice|gb157.2|AU077950 91.1 blastp 715 maize|gb170|AI619081maize 1779 208 rice|gb157.2|AU077950 97.7 blastp 716maize|gb170|AW216176 maize 1780 208 rice|gb157.2|AU077950 87.8 blastp717 maize|gb170|BG841543 maize 1781 208 rice|gb157.2|AU077950 97.7blastp 718 maize|gb170|H89383 maize 1782 208 rice|gb157.2|AU077950 86.9blastp 719 onion|gb162|CF447150 onion 1783 208 rice|gb157.2|AU077950 80blastp 720 pseudoroegneria|gb167|FF343595 pseudoroegneria 1784 208rice|gb157.2|AU077950 94.2 blastp 721 rice|gb170|OS05G36110 rice 1785208 rice|gb157.2|AU077950 86.2 blastp 722 sorghum|gb161.crp|AI783327sorghum 1786 208 rice|gb157.2|AU077950 96.5 blastp 723sorghum|gb161.crp|H89383 sorghum 1787 208 rice|gb157.2|AU077950 87.9blastp 724 sugarcane|gb157.3|BQ479039 sugarcane 1788 208rice|gb157.2|AU077950 98.1 blastp 725 switchgrass|gb167|DN144476switchgrass 1789 208 rice|gb157.2|AU077950 96.9 blastp 726switchgrass|gb167|FE642599 switchgrass 1790 208 rice|gb157.2|AU07795087.5 blastp 727 wheat|gb164|BG909438 wheat 1791 208rice|gb157.2|AU077950 93.4 blastp 728 wheat|gb164|CA497850 wheat 1792208 rice|gb157.2|AU077950 81.9 blastp 729 wheat|gb164|CA658427 wheat1793 208 rice|gb157.2|AU077950 94.2 blastp 730 cenchrus|gb166|EB654920cenchrus 1794 209 sorghum|gb161.xeno|AI901439 82.2 blastp 731maize|gb170|AI855209 maize 1795 209 sorghum|gb161.xeno|AI901439 89.4blastp 732 sugarcane|gb157.3|BU102825 sugarcane 1796 209sorghum|gb161.xeno|AI901439 96.2 blastp 733 switchgrass|gb167|DN146789switchgrass 1797 209 sorghum|gb161.xeno|AI901439 81.2 blastp 734maize|gb170|AI944302 maize 1798 210 sorghum|gb161.xeno|AW052978 93.5blastp 735 sugarcane|gb157.3|BQ534346 sugarcane 1799 210sorghum|gb161.xeno|AW052978 93.5 blastp 736 switchgrass|gb167|FL722616switchgrass 1800 210 sorghum|gb161.xeno|AW052978 86.1 blastp 737barley|gb157.3|BE413281 barley 1801 211 sorghum|gb161.xeno|AW055409 88.3blastp 738 leymus|gb166|EG382167 leymus 1802 211sorghum|gb161.xeno|AW055409 88.5 blastp 739 maize|gb170|AI855325 maize1803 211 sorghum|gb161.xeno|AW055409 93.5 blastp 740rice|gb170|OS01G09010 rice 1804 211 sorghum|gb161.xeno|AW055409 88.8blastp 741 switchgrass|gb167|DN145994 switchgrass 1805 211sorghum|gb161.xeno|AW055409 93 blastp 742 wheat|gb164|BE414789 wheat1806 211 sorghum|gb161.xeno|AW055409 88.5 blastp 743barley|gb157.3|BE437905 barley 1807 212 sorghum|gb161.xeno|AI37219482.43 tblastn 744 brachypodium|gb169|BE437905 brachypodium 1808 212sorghum|gb161.xeno|AI372194 80.2 blastp 745 leymus|gb166|EG394243 leymus1809 212 sorghum|gb161.xeno|AI372194 80.4 blastp 746maize|gb170|BG320821 maize 1810 212 sorghum|gb161.xeno|AI372194 94.5blastp 747 maize|gb170|LLT23330 maize 1811 212sorghum|gb161.xeno|AI372194 96.2 blastp 748 rice|gb170|OS08G45240 rice1812 212 sorghum|gb161.xeno|AI372194 82.2 blastp 749sugarcane|gb157.3|CA073529 sugarcane 1813 212sorghum|gb161.xeno|AI372194 97.9 blastp 750 switchgrass|gb167|DN145055switchgrass 1814 212 sorghum|gb161.xeno|AI372194 94.9 blastp 751wheat|gb164|BE404004 wheat 1815 212 sorghum|gb161.xeno|AI372194 81.59tblastn 752 maize|gb170|AI939746 maize 1816 213 rice|gb157.2|BI805136 80blastp 753 rice|gb170|OS08G44840 rice 1817 213 rice|gb157.2|BI80513699.8 blastp 754 wheat|gb164|BE400051 wheat 1818 213rice|gb157.2|BI805136 80.54 tblastn 755 barley|gb157.3|AL506838 barley1819 214 maize|gb164|AW054475 86.9 blastp 756brachypodium|gb169|BE406703 brachypodium 1820 214 maize|gb164|AW05447590.9 blastp 757 rice|gb170|OS01G13730 rice 1821 214 maize|gb164|AW05447592.7 blastp 758 sorghum|gb161.crp|AI739896 sorghum 1822 214maize|gb164|AW054475 97.8 blastp 759 sugarcane|gb157.3|BQ479038sugarcane 1823 214 maize|gb164|AW054475 97.8 blastp 760switchgrass|gb167|FE622691 switchgrass 1824 214 maize|gb164|AW05447595.1 blastp 761 wheat|gb164|BE406703 wheat 1825 214 maize|gb164|AW05447586.7 blastp 762 apple|gb157.3|AU301405 apple 1826 215soybean|gb166|AW350050 93.1 blastp 763 arabidopsis|gb165|AT2G27600arabidopsis 1827 215 soybean|gb166|AW350050 91 blastp 764b_rapa|gb162|CV546524 b_rapa 1828 215 soybean|gb166|AW350050 90.6 blastp765 b_rapa|gb162|EX019335 b_rapa 1829 215 soybean|gb166|AW350050 89.9blastp 766 barley|gb157.3|BE438944 barley 1830 215soybean|gb166|AW350050 87.5 blastp 767 basilicum|gb157.3|DY330212basilicum 1831 215 soybean|gb166|AW350050 88 blastp 768bean|gb167|CA896847 bean 1832 215 soybean|gb166|AW350050 98.4 blastp 769brachypodium|gb169|BE405668 brachypodium 1833 215 soybean|gb166|AW35005087.9 blastp 770 cacao|gb167|CA794307 cacao 1834 215soybean|gb166|AW350050 93.1 blastp 771 canola|gb161|CD814779 canola 1835215 soybean|gb166|AW350050 88.7 blastp 772 canola|gb161|DY024749 canola1836 215 soybean|gb166|AW350050 90.8 blastp 773castorbean|gb160|EG661556 castorbean 1837 215 soybean|gb166|AW35005093.1 blastp 774 chestnut|gb170|SRR006295S0002595 chestnut 1838 215soybean|gb166|AW350050 92.9 blastp 775 citrus|gb166|CF830344 citrus 1839215 soybean|gb166|AW350050 93.8 blastp 776 cotton|gb164|AI726326 cotton1840 215 soybean|gb166|AW350050 94 blastp 777 cotton|gb164|AI729650cotton 1841 215 soybean|gb166|AW350050 91.5 blastp 778cotton|gb164|AI731487 cotton 1842 215 soybean|gb166|AW350050 89.5 blastp779 cotton|gb164|AI731657 cotton 1843 215 soybean|gb166|AW350050 92.2blastp 780 cowpea|gb166|FF395986 cowpea 1844 215 soybean|gb166|AW35005094.2 blastp 781 iceplant|gb164|AF165422 iceplant 1845 215soybean|gb166|AW350050 91.3 blastp 782 lettuce|gb157.2|DW049083 lettuce1846 215 soybean|gb166|AW350050 90.8 blastp 783 lettuce|gb157.2|DW059917lettuce 1847 215 soybean|gb166|AW350050 83.9 blastp 784maize|gb170|AI615072 maize 1848 215 soybean|gb166|AW350050 89.9 blastp785 maize|gb170|AI714627 maize 1849 215 soybean|gb166|AW350050 89.7blastp 786 medicago|gb157.2|AW329426 medicago 1850 215soybean|gb166|AW350050 91.94 tblastn 787 pine|gb157.2|AL751019 pine 1851215 soybean|gb166|AW350050 88.2 blastp 788 pine|gb157.2|BE643751 pine1852 215 soybean|gb166|AW350050 86.8 blastp 789 poplar|gb170|AI166646poplar 1853 215 soybean|gb166|AW350050 91.7 blastp 790poplar|gb170|BI069748 poplar 1854 215 soybean|gb166|AW350050 90.8 blastp791 poplar|gb170|BI070062 poplar 1855 215 soybean|gb166|AW350050 81.7blastp 792 poplar|gb170|CN549423 poplar 1856 215 soybean|gb166|AW35005081.6 blastp 793 potato|gb157.2|BG096555 potato 1857 215soybean|gb166|AW350050 87.4 blastp 794 potato|gb157.2|BI177056 potato1858 215 soybean|gb166|AW350050 90.8 blastp 795 rice|gb170|OS01G04814rice 1859 215 soybean|gb166|AW350050 90.8 blastp 796sorghum|gb161.crp|BE366383 sorghum 1860 215 soybean|gb166|AW350050 90.6blastp 797 soybean|gb168|AW329426 soybean 1861 215soybean|gb166|AW350050 94 blastp 798 soybean|gb168|AW719488 soybean 1862215 soybean|gb166|AW350050 94 blastp 799 soybean|gb168|AW719867 soybean1863 215 soybean|gb166|AW350050 97.5 blastp 800 spikemoss|gb165|FE429017spikemoss 1864 215 soybean|gb166|AW350050 85.3 blastp 801spruce|gb162|CO217587 spruce 1865 215 soybean|gb166|AW350050 88.2 blastp802 strawberry|gb164|CO816822 strawberry 1866 215 soybean|gb166|AW35005092.4 blastp 803 sugarcane|gb157.3|BQ533539 sugarcane 1867 215soybean|gb166|AW350050 90.6 blastp 804 sunflower|gb162|CD849902sunflower 1868 215 soybean|gb166|AW350050 90.57 tblastn 805sunflower|gb162|DY927633 sunflower 1869 215 soybean|gb166|AW350050 82.3blastp 806 switchgrass|gb167|DN142133 switchgrass 1870 215soybean|gb166|AW350050 90.3 blastp 807 tomato|gb164|AI637361 tomato 1871215 soybean|gb166|AW350050 90.6 blastp 808 tomato|gb164|BE459090 tomato1872 215 soybean|gb166|AW350050 87.2 blastp 809triphysaria|gb164|DR175699 triphysaria 1873 215 soybean|gb166|AW35005090.3 blastp 810 wheat|gb164|BE405903 wheat 1874 215soybean|gb166|AW350050 87.9 blastp 811 maize|gb170|BG316566 maize 1875267 sorghum|gb161.xeno|BE599042 98.87 tblastn 811 maize|gb170|BG316566maize 1875 216 sorghum|gb161.crp|BE599042 96.8 blastp 812rice|gb170|OS11G10420 rice 1876 267 sorghum|gb161.xeno|BE599042 93.57tblastn 812 rice|gb170|OS11G10420 rice 1876 216sorghum|gb161.crp|BE599042 88 blastp 813 aquilegia|gb157.3|DR921243aquilegia 1877 217 maize|gb164|BQ279657 80.7 blastp 814avocado|gb164|CK766314 avocado 1878 217 maize|gb164|BQ279657 80.5 blastp815 brachypodium|gb169|BE492967 brachypodium 1879 217maize|gb164|BQ279657 85.7 blastp 815 brachypodium|gb169|BE492967brachypodium 1879 227 sorghum|gb161.xeno|BQ279657 84.7 blastp 816castorbean|gb160|EE255906 castorbean 1880 217 maize|gb164|BQ279657 81.3blastp 816 castorbean|gb160|EE255906 castorbean 1880 227sorghum|gb161.xeno|BQ279657 81 blastp 817 centaurea|gb166|EH728846centaurea 1881 217 maize|gb164|BQ279657 80.08 tblastn 818chestnut|gb170|SRR006295S0011600 chestnut 1882 217 maize|gb164|BQ27965781.3 blastp 818 chestnut|gb170|SRR006295S0011600 chestnut 1882 227sorghum|gb161.xeno|BQ279657 80.6 blastp 819 citrus|gb166|CB305147 citrus1883 227 sorghum|gb161.xeno|BQ279657 81.4 blastp 819citrus|gb166|CB305147 citrus 1883 217 maize|gb164|BQ279657 81.3 blastp820 cotton|gb164|CO121350 cotton 1884 217 maize|gb164|BQ279657 82.4tblastn 820 cotton|gb164|CO121350 cotton 1884 227sorghum|gb161.xeno|BQ279657 81.41 tblastn 821 kiwi|gb166|FG403767 kiwi1885 217 maize|gb164|BQ279657 80.5 blastp 822 leymus|gb166|EG376319leymus 1886 217 maize|gb164|BQ279657 90.3 blastp 822leymus|gb166|EG376319 leymus 1886 227 sorghum|gb161.xeno|BQ279657 89.6blastp 823 papaya|gb165|EX229221 papaya 1887 217 maize|gb164|BQ27965781.3 blastp 823 papaya|gb165|EX229221 papaya 1887 227sorghum|gb161.xeno|BQ279657 81 blastp 824 potato|gb157.2|BE341318 potato1888 217 maize|gb164|BQ279657 81.3 blastp 824 potato|gb157.2|BE341318potato 1888 227 sorghum|gb161.xeno|BQ279657 81.3 blastp 825pseudoroegneria|gb167|FF342296 pseudoroegneria 1889 217maize|gb164|BQ279657 89.9 blastp 825 pseudoroegneria|gb167|FF342296pseudoroegneria 1889 227 sorghum|gb161.xeno|BQ279657 89.2 blastp 826rice|gb170|OS03G21914 rice 1890 217 maize|gb164|BQ279657 90.6 blastp 826rice|gb170|OS03G21914 rice 1890 227 sorghum|gb161.xeno|BQ279657 89.9blastp 827 rice|gb170|OS03G50620 rice 1891 217 maize|gb164|BQ279657 88.2blastp 827 rice|gb170|OS03G50620 rice 1891 227sorghum|gb161.xeno|BQ279657 87.2 blastp 828 sugarcane|gb157.3|BQ536934sugarcane 1892 227 sorghum|gb161.xeno|BQ279657 99.3 blastp 828sugarcane|gb157.3|BQ536934 sugarcane 1892 217 maize|gb164|BQ279657 98.1blastp 829 sugarcane|gb157.3|CA096803 sugarcane 1893 227sorghum|gb161.xeno|BQ279657 98.9 blastp 829 sugarcane|gb157.3|CA096803sugarcane 1893 217 maize|gb164|BQ279657 97.8 blastp 830sunflower|gb162|BU018368 sunflower 1894 217 maize|gb164|BQ279657 80.2blastp 831 switchgrass|gb167|FL773351 switchgrass 1895 227sorghum|gb161.xeno|BQ279657 94.8 blastp 831 switchgrass|gb167|FL773351switchgrass 1895 217 maize|gb164|BQ279657 94 blastp 832tobacco|gb162|DV158876 tobacco 1896 217 maize|gb164|BQ279657 80.9 blastp832 tobacco|gb162|DV158876 tobacco 1896 227 sorghum|gb161.xeno|BQ27965780.2 blastp 833 tomato|gb164|BG124565 tomato 1897 217maize|gb164|BQ279657 80.9 blastp 833 tomato|gb164|BG124565 tomato 1897227 sorghum|gb161.xeno|BQ279657 80.6 blastp 834 wheat|gb164|BE492967wheat 1898 217 maize|gb164|BQ279657 89.9 blastp 834 wheat|gb164|BE492967wheat 1898 227 sorghum|gb161.xeno|BQ279657 89.2 blastp 835wheat|gb164|BQ168983 wheat 1899 217 maize|gb164|BQ279657 90.3 blastp 835wheat|gb164|BQ168983 wheat 1899 227 sorghum|gb161.xeno|BQ279657 89.6blastp 836 fescue|gb161|CK801026 fescue 1900 218 barley|gb157.2|AJ23440887 blastp 837 pseudoroegneria|gb167|FF340368 pseudoroegneria 1901 218barley|gb157.2|AJ234408 92.1 blastp 838 rice|gb170|OS07G05360 rice 1902218 barley|gb157.2|AJ234408 81.2 blastp 839 wheat|gb164|BE213379 wheat1903 218 barley|gb157.2|AJ234408 92.1 blastp 840 wheat|gb164|BE401132wheat 1904 218 barley|gb157.2|AJ234408 90 blastp 841wheat|gb164|BE401288 wheat 1905 218 barley|gb157.2|AJ234408 90.7 blastp842 wheat|gb164|CA654680 wheat 1906 218 barley|gb157.2|AJ234408 84.89tblastn 843 wheat|gb164|CA695915 wheat 1907 218 barley|gb157.2|AJ23440888.49 tblastn 844 maize|gb170|AW433364 maize 1908 219sorghum|gb161.xeno|AW923729 87.9 blastp 845 sorghum|gb161.crp|AW922411sorghum 1909 219 sorghum|gb161.xeno|AW923729 81.1 blastp 846sugarcane|gb157.3|CA068925 sugarcane 1910 219sorghum|gb161.xeno|AW923729 91.1 blastp 847 switchgrass|gb167|DN144528switchgrass 1911 219 sorghum|gb161.xeno|AW923729 86.8 blastp 848switchgrass|gb167|DN144587 switchgrass 1912 219sorghum|gb161.xeno|AW923729 85.8 blastp 849 switchgrass|gb167|FL758275switchgrass 1913 219 sorghum|gb161.xeno|AW923729 80.1 blastp 850castorbean|gb160|MDL29813M001539 castorbean 1914 221arabidopsis|gb157.2|AT1G13980 83.3 blastp 851 poplar|gb170|BU886496poplar 1915 221 arabidopsis|gb157.2|AT1G13980 82 blastp 852soybean|gb168|BM308552 soybean 1916 221 arabidopsis|gb157.2|AT1G1398081.2 blastp 853 maize|gb170|BM072861 maize 1917 226sorghum|gb161.xeno|BI139559 95.5 blastp 854 rice|gb170|OS01G07200 rice1918 226 sorghum|gb161.xeno|BI139559 83.5 blastp 855sugarcane|gb157.3|CA112539 sugarcane 1919 226sorghum|gb161.xeno|BI139559 88.25 tblastn 856 barley|gb157.3|BE412997barley 1920 228 sorghum|gb161.xeno|AF019147 84.1 blastp 857brachypodium|gb169|BE403874 brachypodium 1921 228sorghum|gb161.xeno|AF019147 83 blastp 858 fescue|gb161|DT680716 fescue1922 228 sorghum|gb161.xeno|AF019147 81 blastp 859 leymus|gb166|CN466335leymus 1923 228 sorghum|gb161.xeno|AF019147 83.4 blastp 860maize|gb170|AF019147 maize 1924 228 sorghum|gb161.xeno|AF019147 91.7blastp 861 maize|gb170|AI948311 maize 1925 228sorghum|gb161.xeno|AF019147 90 blastp 862 rice|gb170|OS04G55650 rice1926 228 sorghum|gb161.xeno|AF019147 85.2 blastp 863sugarcane|gb157.3|BQ536348 sugarcane 1927 228sorghum|gb161.xeno|AF019147 97.9 blastp 864 switchgrass|gb167|DN140659switchgrass 1928 228 sorghum|gb161.xeno|AF019147 91.8 blastp 865switchgrass|gb167|DN141292 switchgrass 1929 228sorghum|gb161.xeno|AF019147 90.8 blastp 866 wheat|gb164|BE403874 wheat1930 228 sorghum|gb161.xeno|AF019147 83.9 blastp 867wheat|gb164|BE405077 wheat 1931 228 sorghum|gb161.xeno|AF019147 84.5blastp 868 b_rapa|gb162|EX027120 b_rapa 1932 229 canola|gb161|EE55984394.1 blastp 869 brachypodium|gb169|BF202681 brachypodium 1933 230barley|gb157.3|BE420701 90.9 blastp 870 maize|gb170|LLAI629913 maize1934 230 barley|gb157.3|BE420701 87 blastp 871pseudoroegneria|gb167|FF340034 pseudoroegneria 1935 230barley|gb157.3|BE420701 96.8 blastp 872 rice|gb170|OS07G44660 rice 1936230 barley|gb157.3|BE420701 88.6 blastp 873 sorghum|gb161.crp|AW282627sorghum 1937 230 barley|gb157.3|BE420701 88.3 blastp 874switchgrass|gb167|FE609054 switchgrass 1938 230 barley|gb157.3|BE42070188.1 blastp 875 brachypodium|gb169|BE421829 brachypodium 1939 231barley|gb157.3|BE420701 90.2 blastp 875 brachypodium|gb169|BE421829brachypodium 1939 235 rice|gb157.2|AU057884 82.6 blastp 875brachypodium|gb169|BE421829 brachypodium 1939 261sorghum|gb161.xeno|AI622209 81.1 blastp 876 fescue|gb161|DT679850 fescue1940 231 barley|gb157.3|BE421829 95.3 blastp 876 fescue|gb161|DT679850fescue 1940 235 rice|gb157.2|AU057884 84.1 blastp 876fescue|gb161|DT679850 fescue 1940 261 sorghum|gb161.xeno|AI622209 81.6blastp 877 leymus|gb166|EG396605 leymus 1941 231 barley|gb157.3|BE42182996.3 blastp 877 leymus|gb166|EG396605 leymus 1941 235rice|gb157.2|AU057884 83.6 blastp 877 leymus|gb166|EG396605 leymus 1941261 sorghum|gb161.xeno|AI622209 83.1 blastp 878 maize|gb170|AI622209maize 1942 261 sorghum|gb161.xeno|AI622209 94 blastp 878maize|gb170|AI622209 maize 1942 235 rice|gb157.2|AU057884 82.9 blastp878 maize|gb170|AI622209 maize 1942 231 barley|gb157.3|BE421829 81.8blastp 879 sugarcane|gb157.3|CA123704 sugarcane 1943 261sorghum|gb161.xeno|AI622209 93.5 blastp 879 sugarcane|gb157.3|CA123704sugarcane 1943 231 barley|gb157.3|BE421829 86.5 blastp 879sugarcane|gb157.3|CA123704 sugarcane 1943 235 rice|gb157.2|AU057884 84.6blastp 880 switchgrass|gb167|FE619680 switchgrass 1944 261sorghum|gb161.xeno|AI622209 91 blastp 880 switchgrass|gb167|FE619680switchgrass 1944 235 rice|gb157.2|AU057884 85.4 blastp 880switchgrass|gb167|FE619680 switchgrass 1944 231 barley|gb157.3|BE42182982.7 blastp 881 switchgrass|gb167|FE630609 switchgrass 1945 261sorghum|gb161.xeno|AI622209 89.6 blastp 881 switchgrass|gb167|FE630609switchgrass 1945 235 rice|gb157.2|AU057884 84 blastp 881switchgrass|gb167|FE630609 switchgrass 1945 231 barley|gb157.3|BE42182981.9 blastp 882 wheat|gb164|BE497607 wheat 1946 231barley|gb157.3|BE421829 96.8 blastp 882 wheat|gb164|BE497607 wheat 1946235 rice|gb157.2|AU057884 84.6 blastp 882 wheat|gb164|BE497607 wheat1946 261 sorghum|gb161.xeno|AI622209 82.6 blastp 883wheat|gb164|BF428660 wheat 1947 231 barley|gb157.3|BE421829 96.8 blastp883 wheat|gb164|BF428660 wheat 1947 235 rice|gb157.2|AU057884 85.6blastp 883 wheat|gb164|BF428660 wheat 1947 261sorghum|gb161.xeno|AI622209 82.6 blastp 884 barley|gb157.3|BE411922barley 1948 232 sorghum|gb161.xeno|AA011880 81 blastp 885brachypodium|gb169|BE398696 brachypodium 1949 232sorghum|gb161.xeno|AA011880 82.3 blastp 886 cenchrus|gb166|EB652789cenchrus 1950 232 sorghum|gb161.xeno|AA011880 92.3 blastp 887cotton|gb164|DT574337 cotton 1951 232 sorghum|gb161.xeno|AA011880 95.9blastp 888 leymus|gb166|CN465754 leymus 1952 232sorghum|gb161.xeno|AA011880 81.1 blastp 889 maize|gb170|AA011880 maize1953 232 sorghum|gb161.xeno|AA011880 95.9 blastp 890maize|gb170|LLCD979368 maize 1954 232 sorghum|gb161.xeno|AA011880 95.9blastp 891 pseudoroegneria|gb167|FF344484 pseudoroegneria 1955 232sorghum|gb161.xeno|AA011880 83.3 blastp 892 rice|gb170|OS07G46750 rice1956 232 sorghum|gb161.xeno|AA011880 87.9 blastp 893sugarcane|gb157.3|BQ535840 sugarcane 1957 232sorghum|gb161.xeno|AA011880 97.3 blastp 894 sugarcane|gb157.3|BQ536355sugarcane 1958 232 sorghum|gb161.xeno|AA011880 94 blastp 895sugarcane|gb157.3|CA065609 sugarcane 1959 232sorghum|gb161.xeno|AA011880 83.94 tblastn 896 sugarcane|gb157.3|CA075754sugarcane 1960 232 sorghum|gb161.xeno|AA011880 93.3 blastp 897sugarcane|gb157.3|CA078921 sugarcane 1961 232sorghum|gb161.xeno|AA011880 98.6 blastp 898 switchgrass|gb167|DN141728switchgrass 1962 232 sorghum|gb161.xeno|AA011880 91.5 blastp 899switchgrass|gb167|DN145078 switchgrass 1963 232sorghum|gb161.xeno|AA011880 92.4 blastp 900 wheat|gb164|BE398306 wheat1964 232 sorghum|gb161.xeno|AA011880 82.8 blastp 901wheat|gb164|BE398696 wheat 1965 232 sorghum|gb161.xeno|AA011880 81.4blastp 902 wheat|gb164|BE423010 wheat 1966 232sorghum|gb161.xeno|AA011880 82.8 blastp 903 wheat|gb164|CA484184 wheat1967 232 sorghum|gb161.xeno|AA011880 97.7 blastp 904barley|gb157.3|BE413465 barley 1968 233 rice|gb157.2|BE229552 87.4blastp 905 brachypodium|gb169|BE413465 brachypodium 1969 233rice|gb157.2|BE229552 86.5 blastp 906 leymus|gb166|EG379179 leymus 1970233 rice|gb157.2|BE229552 87.1 blastp 907 maize|gb170|T26952 maize 1971233 rice|gb157.2|BE229552 87.7 blastp 908 sorghum|gb161.crp|BG549557sorghum 1972 233 rice|gb157.2|BE229552 87.7 blastp 909sugarcane|gb157.3|CA099583 sugarcane 1973 233 rice|gb157.2|BE229552 88.3blastp 910 switchgrass|gb167|FE610789 switchgrass 1974 233rice|gb157.2|BE229552 85.6 blastp 911 switchgrass|gb167|FL748149switchgrass 1975 233 rice|gb157.2|BE229552 88 blastp 912wheat|gb164|BE430330 wheat 1976 233 rice|gb157.2|BE229552 86.83 tblastn913 wheat|gb164|BE490164 wheat 1977 233 rice|gb157.2|BE229552 87.1blastp 914 wheat|gb164|BF201086 wheat 1978 233 rice|gb157.2|BE22955287.4 blastp 915 amborella|gb166|CD484126 amborella 1979 234rice|gb157.2|BE039784 96 blastp 916 amborella|gb166|CK760819 amborella1980 234 rice|gb157.2|BE039784 97.4 blastp 917antirrhinum|gb166|AJ558674 antirrhinum 1981 234 rice|gb157.2|BE03978489.4 blastp 918 antirrhinum|gb166|AJ559850 antirrhinum 1982 234rice|gb157.2|BE039784 91.4 blastp 919 antirrhinum|gb166|AJ787300antirrhinum 1983 234 rice|gb157.2|BE039784 91.4 blastp 920antirrhinum|gb166|AJ789533 antirrhinum 1984 234 rice|gb157.2|BE03978491.4 blastp 921 apple|gb157.3|CN489349 apple 1985 234rice|gb157.2|BE039784 90.7 blastp 922 apple|gb157.3|CN496576 apple 1986234 rice|gb157.2|BE039784 91.4 blastp 923 apple|gb157.3|CN995013 apple1987 234 rice|gb157.2|BE039784 90.7 blastp 924 apricot|gb157.2|CB819597apricot 1988 234 rice|gb157.2|BE039784 92.7 blastp 925apricot|gb157.2|CV044080 apricot 1989 234 rice|gb157.2|BE039784 93.4blastp 926 aquilegia|gb157.3|DR915026 aquilegia 1990 234rice|gb157.2|BE039784 94.7 blastp 927 arabidopsis|gb165|AT2G36160arabidopsis 1991 234 rice|gb157.2|BE039784 90.7 blastp 928arabidopsis|gb165|AT3G11510 arabidopsis 1992 234 rice|gb157.2|BE03978491.4 blastp 929 arabidopsis|gb165|AT3G52580 arabidopsis 1993 234rice|gb157.2|BE039784 91.4 blastp 930 artemisia|gb164|EY033322 artemisia1994 234 rice|gb157.2|BE039784 89.4 blastp 931 artemisia|gb164|EY038655artemisia 1995 234 rice|gb157.2|BE039784 88.1 blastp 932artemisia|gb164|EY050701 artemisia 1996 234 rice|gb157.2|BE039784 89.4blastp 933 avocado|gb164|CK753882 avocado 1997 234 rice|gb157.2|BE03978493.4 blastp 934 b_juncea|gb164|EVGN00033609170815 b_juncea 1998 234rice|gb157.2|BE039784 92.7 blastp 935 b_juncea|gb164|EVGN00191625522759b_juncea 1999 234 rice|gb157.2|BE039784 92.7 blastp 936b_juncea|gb164|EVGN00422623890637 b_juncea 2000 234rice|gb157.2|BE039784 84.8 blastp 937 b_juncea|gb164|EVGN00544912222373b_juncea 2001 234 rice|gb157.2|BE039784 93.4 blastp 938b_juncea|gb164|EVGN00716011751939 b_juncea 2002 234rice|gb157.2|BE039784 92.1 blastp 939 b_juncea|gb164|EVGN00888211982122b_juncea 2003 234 rice|gb157.2|BE039784 92.1 blastp 940b_juncea|gb164|EVGN01248609033239 b_juncea 2004 234rice|gb157.2|BE039784 92.7 blastp 941 b_oleracea|gb161|DY026232b_oleracea 2005 234 rice|gb157.2|BE039784 93.4 blastp 942b_oleracea|gb161|DY026495 b_oleracea 2006 234 rice|gb157.2|BE039784 92.7blastp 943 b_oleracea|gb161|DY026867 b_oleracea 2007 234rice|gb157.2|BE039784 93.4 blastp 944 b_oleracea|gb161|DY027139b_oleracea 2008 234 rice|gb157.2|BE039784 92.7 blastp 945b_oleracea|gb161|DY028093 b_oleracea 2009 234 rice|gb157.2|BE039784 92.7blastp 946 b_oleracea|gb161|ES942246 b_oleracea 2010 234rice|gb157.2|BE039784 92.7 blastp 947 b_rapa|gb162|BG544390 b_rapa 2011234 rice|gb157.2|BE039784 92.7 blastp 948 b_rapa|gb162|CA992255 b_rapa2012 234 rice|gb157.2|BE039784 92.7 blastp 949 b_rapa|gb162|CV433769b_rapa 2013 234 rice|gb157.2|BE039784 92.7 blastp 950b_rapa|gb162|CV433783 b_rapa 2014 234 rice|gb157.2|BE039784 93.4 blastp951 b_rapa|gb162|CX265694 b_rapa 2015 234 rice|gb157.2|BE039784 93.4blastp 952 b_rapa|gb162|CX270276 b_rapa 2016 234 rice|gb157.2|BE03978492.7 blastp 953 b_rapa|gb162|CX270426 b_rapa 2017 234rice|gb157.2|BE039784 93.4 blastp 954 b_rapa|gb162|DY008989 b_rapa 2018234 rice|gb157.2|BE039784 93.4 blastp 955 b_rapa|gb162|EE525926 b_rapa2019 234 rice|gb157.2|BE039784 92.7 blastp 956 b_rapa|gb162|L33661b_rapa 2020 234 rice|gb157.2|BE039784 93.4 blastp 957banana|gb167|DN240239 banana 2021 234 rice|gb157.2|BE039784 96.7 blastp958 banana|gb167|ES433381 banana 2022 234 rice|gb157.2|BE039784 96.7blastp 959 banana|gb167|FF558372 banana 2023 234 rice|gb157.2|BE03978496.7 blastp 960 banana|gb167|FF558518 banana 2024 234rice|gb157.2|BE039784 97.4 blastp 961 banana|gb167|FL662140 banana 2025234 rice|gb157.2|BE039784 91.4 blastp 962 barley|gb157.3|AL501882 barley2026 234 rice|gb157.2|BE039784 96 blastp 963 barley|gb157.3|BE412576barley 2027 234 rice|gb157.2|BE039784 97.4 blastp 964barley|gb157.3|BQ768399 barley 2028 234 rice|gb157.2|BE039784 82.8blastp 965 barley|gb157.3|DN183050 barley 2029 234 rice|gb157.2|BE03978480.9 blastp 966 basilicum|gb157.3|DY331402 basilicum 2030 234rice|gb157.2|BE039784 91.4 blastp 967 basilicum|gb157.3|DY344099basilicum 2031 234 rice|gb157.2|BE039784 90.1 blastp 968bean|gb167|CA897110 bean 2032 234 rice|gb157.2|BE039784 91.4 blastp 969bean|gb167|CA897113 bean 2033 234 rice|gb157.2|BE039784 91.4 blastp 970beet|gb162|BQ060487 beet 2034 234 rice|gb157.2|BE039784 94 blastp 971brachypodium|gb169|BE398957 brachypodium 2035 234 rice|gb157.2|BE03978494.04 tblastn 972 brachypodium|gb169|BE402469 brachypodium 2036 234rice|gb157.2|BE039784 97.35 tblastn 973 brachypodium|gb169|BE403589brachypodium 2037 234 rice|gb157.2|BE039784 95.4 blastp 974brachypodium|gb169|BE406789 brachypodium 2038 234 rice|gb157.2|BE03978496.7 blastp 975 bruguiera|gb166|BP949576 bruguiera 2039 234rice|gb157.2|BE039784 91.39 tblastn 976 cacao|gb167|CA796567 cacao 2040234 rice|gb157.2|BE039784 93.4 blastp 977 cacao|gb167|CU473326 cacao2041 234 rice|gb157.2|BE039784 92.1 blastp 978 canola|gb161|AY196093canola 2042 234 rice|gb157.2|BE039784 93.4 blastp 979canola|gb161|CD811632 canola 2043 234 rice|gb157.2|BE039784 93.4 blastp980 canola|gb161|CD812906 canola 2044 234 rice|gb157.2|BE039784 92.7blastp 981 canola|gb161|CD820445 canola 2045 234 rice|gb157.2|BE03978493.4 blastp 982 canola|gb161|CD822523 canola 2046 234rice|gb157.2|BE039784 92.7 blastp 983 canola|gb161|CD823758 canola 2047234 rice|gb157.2|BE039784 92.7 blastp 984 canola|gb161|CD827084 canola2048 234 rice|gb157.2|BE039784 92.7 blastp 985 canola|gb161|CD829044canola 2049 234 rice|gb157.2|BE039784 92.7 blastp 986canola|gb161|CD840491 canola 2050 234 rice|gb157.2|BE039784 92.7 blastp987 canola|gb161|CN730264 canola 2051 234 rice|gb157.2|BE039784 93.4blastp 988 canola|gb161|CN731838 canola 2052 234 rice|gb157.2|BE03978492.7 blastp 989 canola|gb161|CX190513 canola 2053 234rice|gb157.2|BE039784 92.7 blastp 990 canola|gb161|CX280454 canola 2054234 rice|gb157.2|BE039784 92.7 blastp 991 canola|gb161|CX280565 canola2055 234 rice|gb157.2|BE039784 92.7 blastp 992 canola|gb161|H07559canola 2056 234 rice|gb157.2|BE039784 92.7 blastp 993cassava|gb164|CK647007 cassava 2057 234 rice|gb157.2|BE039784 95.4blastp 994 cassava|gb164|CK650413 cassava 2058 234 rice|gb157.2|BE03978495.4 blastp 995 cassava|gb164|CK652715 cassava 2059 234rice|gb157.2|BE039784 95.4 blastp 996 castorbean|gb160|MDL29693M002016castorbean 2060 234 rice|gb157.2|BE039784 93.4 blastp 997castorbean|gb160|T14945 castorbean 2061 234 rice|gb157.2|BE039784 93.4blastp 998 catharanthus|gb166|EG556977 catharanthus 2062 234rice|gb157.2|BE039784 88.7 blastp 999 catharanthus|gb166|EG557933catharanthus 2063 234 rice|gb157.2|BE039784 93.4 blastp 1000cenchrus|gb166|EB656767 cenchrus 2064 234 rice|gb157.2|BE039784 94.7blastp 1001 cenchrus|gb166|EB664187 cenchrus 2065 234rice|gb157.2|BE039784 97.4 blastp 1002 centaurea|gb166|EH724794centaurea 2066 234 rice|gb157.2|BE039784 91.4 blastp 1003centaurea|gb166|EH739148 centaurea 2067 234 rice|gb157.2|BE039784 91.4blastp 1004 centaurea|gb166|EH748001 centaurea 2068 234rice|gb157.2|BE039784 90.7 blastp 1005 centaurea|gb166|EH753801centaurea 2069 234 rice|gb157.2|BE039784 91.4 blastp 1006centaurea|gb166|EH780000 centaurea 2070 234 rice|gb157.2|BE039784 91.4blastp 1007 cherry|gb157.2|EE488074 cherry 2071 234rice|gb157.2|BE039784 92.05 tblastn 1008chestnut|gb170|SRR006295S0002784 chestnut 2072 234 rice|gb157.2|BE03978494 blastp 1009 chestnut|gb170|SRR006295S0004532 chestnut 2073 234rice|gb157.2|BE039784 93.4 blastp 1010 chestnut|gb170|SRR006295S0010942chestnut 2074 234 rice|gb157.2|BE039784 94 blastp 1011chlamydomonas|gb162|AW676072 chlamydomonas 2075 234rice|gb157.2|BE039784 85.6 blastp 1012 cichorium|gb166|DT211087cichorium 2076 234 rice|gb157.2|BE039784 89.4 blastp 1013cichorium|gb166|DT214005 cichorium 2077 234 rice|gb157.2|BE039784 91.4blastp 1014 cichorium|gb166|EL356717 cichorium 2078 234rice|gb157.2|BE039784 91.4 blastp 1015 cichorium|gb166|EL365574cichorium 2079 234 rice|gb157.2|BE039784 91.4 blastp 1016citrus|gb166|BQ623292 citrus 2080 234 rice|gb157.2|BE039784 94.7 blastp1017 citrus|gb166|BQ624114 citrus 2081 234 rice|gb157.2|BE039784 93.4blastp 1018 coffea|gb157.2|BQ449109 coffea 2082 234rice|gb157.2|BE039784 93.4 blastp 1019 coffea|gb157.2|DV673676 coffea2083 234 rice|gb157.2|BE039784 92.7 blastp 1020 cotton|gb164|AI726845cotton 2084 234 rice|gb157.2|BE039784 92.7 blastp 1021cotton|gb164|AI730068 cotton 2085 234 rice|gb157.2|BE039784 92.1 blastp1022 cotton|gb164|BE054711 cotton 2086 234 rice|gb157.2|BE039784 92.7blastp 1023 cotton|gb164|BF271677 cotton 2087 234 rice|gb157.2|BE03978492.1 blastp 1024 cotton|gb164|CD485874 cotton 2088 234rice|gb157.2|BE039784 81.46 tblastn 1025 cotton|gb164|DV849004 cotton2089 234 rice|gb157.2|BE039784 92.1 blastp 1026 cotton|gb164|ES792938cotton 2090 234 rice|gb157.2|BE039784 92.1 blastp 1027cowpea|gb166|FC459672 cowpea 2091 234 rice|gb157.2|BE039784 91.4 blastp1028 cowpea|gb166|FF384317 cowpea 2092 234 rice|gb157.2|BE039784 81.6blastp 1029 cowpea|gb166|FF385803 cowpea 2093 234 rice|gb157.2|BE03978491.4 blastp 1030 cowpea|gb166|FF389079 cowpea 2094 234rice|gb157.2|BE039784 83.4 blastp 1031 cowpea|gb166|FF391295 cowpea 2095234 rice|gb157.2|BE039784 90.7 blastp 1032 cryptomeria|gb166|BP173938cryptomeria 2096 234 rice|gb157.2|BE039784 92.7 blastp 1033cryptomeria|gb166|BW994122 cryptomeria 2097 234 rice|gb157.2|BE03978492.7 blastp 1034 cycas|gb166|EX923616 cycas 2098 234rice|gb157.2|BE039784 92.1 blastp 1035 cycas|gb166|EX924938 cycas 2099234 rice|gb157.2|BE039784 91.39 tblastn 1036 cynara|gb167|GE586142cynara 2100 234 rice|gb157.2|BE039784 91.4 blastp 1037cynara|gb167|GE586173 cynara 2101 234 rice|gb157.2|BE039784 91.4 blastp1038 cynara|gb167|GE591726 cynara 2102 234 rice|gb157.2|BE039784 90.1blastp 1039 dandelion|gb161|DY804347 dandelion 2103 234rice|gb157.2|BE039784 91.4 blastp 1040 dandelion|gb161|DY807877dandelion 2104 234 rice|gb157.2|BE039784 91.4 blastp 1041eucalyptus|gb166|CB967799 eucalyptus 2105 234 rice|gb157.2|BE039784 94.7blastp 1042 eucalyptus|gb166|CT980941 eucalyptus 2106 234rice|gb157.2|BE039784 94.7 blastp 1043 fescue|gb161|DT679829 fescue 2107234 rice|gb157.2|BE039784 98 blastp 1044 fescue|gb161|DT682674 fescue2108 234 rice|gb157.2|BE039784 96 blastp 1045 fescue|gb161|DT688310fescue 2109 234 rice|gb157.2|BE039784 98 blastp 1046flax|gb157.3|CV478813 flax 2110 234 rice|gb157.2|BE039784 89.4 tblastn1047 ginger|gb164|DY372231 ginger 2111 234 rice|gb157.2|BE039784 97.4blastp 1048 grape|gb160|BQ796073 grape 2112 234 rice|gb157.2|BE03978494.7 blastp 1049 grape|gb160|BQ796330 grape 2113 234rice|gb157.2|BE039784 93.4 blastp 1050 grape|gb160|BQ800180 grape 2114234 rice|gb157.2|BE039784 94 blastp 1051 iceplant|gb164|BE034755iceplant 2115 234 rice|gb157.2|BE039784 93.4 blastp 1052iceplant|gb164|CA833881 iceplant 2116 234 rice|gb157.2|BE039784 92.7blastp 1053 ipomoea|gb157.2|BJ554031 ipomoea 2117 234rice|gb157.2|BE039784 94.7 blastp 1054 ipomoea|gb157.2|BJ555694 ipomoea2118 234 rice|gb157.2|BE039784 92.72 tblastn 1055ipomoea|gb157.2|BJ557693 ipomoea 2119 234 rice|gb157.2|BE039784 94.7blastp 1056 ipomoea|gb157.2|BU691365 ipomoea 2120 234rice|gb157.2|BE039784 94 blastp 1057 kiwi|gb166|FG404658 kiwi 2121 234rice|gb157.2|BE039784 92.7 blastp 1058 kiwi|gb166|FG404746 kiwi 2122 234rice|gb157.2|BE039784 92.1 blastp 1059 kiwi|gb166|FG408063 kiwi 2123 234rice|gb157.2|BE039784 94 blastp 1060 lettuce|gb157.2|DW078606 lettuce2124 234 rice|gb157.2|BE039784 91.4 blastp 1061 leymus|gb166|EG388410leymus 2125 234 rice|gb157.2|BE039784 96 blastp 1062liriodendron|gb166|CK743464 liriodendron 2126 234 rice|gb157.2|BE03978494.7 blastp 1063 liriodendron|gb166|CO998653 liriodendron 2127 234rice|gb157.2|BE039784 93.4 blastp 1064 lotus|gb157.2|AI967817 lotus 2128234 rice|gb157.2|BE039784 90.1 blastp 1065 lotus|gb157.2|CB826697 lotus2129 234 rice|gb157.2|BE039784 89.4 blastp 1066 lovegrass|gb167|DN480258lovegrass 2130 234 rice|gb157.2|BE039784 99.3 blastp 1067lovegrass|gb167|EH183996 lovegrass 2131 234 rice|gb157.2|BE039784 98.7blastp 1068 maize|gb170|AI612306 maize 2132 234 rice|gb157.2|BE03978497.4 blastp 1069 maize|gb170|AI967032 maize 2133 234rice|gb157.2|BE039784 98.7 blastp 1070 maize|gb170|AI979679 maize 2134234 rice|gb157.2|BE039784 85.71 tblastn 1071 maize|gb170|AW054617 maize2135 234 rice|gb157.2|BE039784 97.4 blastp 1072 maize|gb170|AW165569maize 2136 234 rice|gb157.2|BE039784 97.4 blastp 1073maize|gb170|LLBU037867 maize 2137 234 rice|gb157.2|BE039784 94.7 tblastn1074 maize|gb170|LLDQ244878 maize 2138 234 rice|gb157.2|BE039784 92.7blastp 1075 maize|gb170|LLDQ245962 maize 2139 234 rice|gb157.2|BE03978492.1 blastp 1076 maize|gb170|T18275 maize 2140 234 rice|gb157.2|BE03978497.4 blastp 1077 marchantia|gb166|BJ841500 marchantia 2141 234rice|gb157.2|BE039784 89.4 blastp 1078 marchantia|gb166|C95799marchantia 2142 234 rice|gb157.2|BE039784 92.1 blastp 1079medicago|gb157.2|AA660312 medicago 2143 234 rice|gb157.2|BE039784 89.4blastp 1080 medicago|gb157.2|AA660491 medicago 2144 234rice|gb157.2|BE039784 88.7 blastp 1081 melon|gb165|AM713905 melon 2145234 rice|gb157.2|BE039784 94 blastp 1082 melon|gb165|AM719737 melon 2146234 rice|gb157.2|BE039784 84.1 blastp 1083 melon|gb165|AM719902 melon2147 234 rice|gb157.2|BE039784 92.76 tblastn 1084 melon|gb165|EB714362melon 2148 234 rice|gb157.2|BE039784 94 blastp 1085mesostigma|gb166|DN254866 mesostigma 2149 234 rice|gb157.2|BE039784 86.3blastp 1086 millet|gb161|CD724748 millet 2150 234 rice|gb157.2|BE03978496 blastp 1087 millet|gb161|CD725398 millet 2151 234rice|gb157.2|BE039784 92.72 tblastn 1088 nuphar|gb166|CD475044 nuphar2152 234 rice|gb157.2|BE039784 94 blastp 1089 nuphar|gb166|CK757845nuphar 2153 234 rice|gb157.2|BE039784 94 blastp 1090nuphar|gb166|CK767949 nuphar 2154 234 rice|gb157.2|BE039784 93.4 blastp1091 oak|gb170|DB996865 oak 2155 234 rice|gb157.2|BE039784 93.4 blastp1092 oak|gb170|DB998068 oak 2156 234 rice|gb157.2|BE039784 94 blastp1093 oak|gb170|DN949738 oak 2157 234 rice|gb157.2|BE039784 94 blastp1094 oil_palm|gb166|EL681750 oil_palm 2158 234 rice|gb157.2|BE039784 96blastp 1095 oil_palm|gb166|EL930220 oil_palm 2159 234rice|gb157.2|BE039784 96 blastp 1096 oil_palm|gb166|EL930363 oil_palm2160 234 rice|gb157.2|BE039784 96 blastp 1097 onion|gb162|BQ580074 onion2161 234 rice|gb157.2|BE039784 96 blastp 1098 papaya|gb165|EX231620papaya 2162 234 rice|gb157.2|BE039784 92.7 blastp 1099papaya|gb165|EX252393 papaya 2163 234 rice|gb157.2|BE039784 92.7 blastp1100 peach|gb157.2|BU040848 peach 2164 234 rice|gb157.2|BE039784 93.4blastp 1101 peanut|gb167|CD037918 peanut 2165 234 rice|gb157.2|BE03978492.1 blastp 1102 peanut|gb167|CX018155 peanut 2166 234rice|gb157.2|BE039784 92.1 blastp 1103 pepper|gb157.2|BM061978 pepper2167 234 rice|gb157.2|BE039784 92.1 blastp 1104 pepper|gb157.2|BM062219pepper 2168 234 rice|gb157.2|BE039784 92.7 blastp 1105pepper|gb157.2|BM066627 pepper 2169 234 rice|gb157.2|BE039784 92.7blastp 1106 periwinkle|gb164|EG556977 periwinkle 2170 234rice|gb157.2|BE039784 88.7 blastp 1107 periwinkle|gb164|EG557933periwinkle 2171 234 rice|gb157.2|BE039784 93.4 blastp 1108physcomitrella|gb157|AW127039 physcomitrella 2172 234rice|gb157.2|BE039784 89.4 tblastn 1109 physcomitrella|gb157|BQ827306physcomitrella 2173 234 rice|gb157.2|BE039784 88.7 blastp 1110pine|gb157.2|AW010184 pine 2174 234 rice|gb157.2|BE039784 92.7 blastp1111 pine|gb157.2|BX248872 pine 2175 234 rice|gb157.2|BE039784 93.4blastp 1112 pine|gb157.2|BX251919 pine 2176 234 rice|gb157.2|BE03978492.7 blastp 1113 pine|gb157.2|DR102094 pine 2177 234rice|gb157.2|BE039784 82.1 blastp 1114 pine|gb157.2|H75266 pine 2178 234rice|gb157.2|BE039784 92.7 blastp 1115 poplar|gb170|AI162468 poplar 2179234 rice|gb157.2|BE039784 96 blastp 1116 poplar|gb170|AI163154 poplar2180 234 rice|gb157.2|BE039784 93.4 blastp 1117 poplar|gb170|AI164614poplar 2181 234 rice|gb157.2|BE039784 94 blastp 1118poplar|gb170|AI164759 poplar 2182 234 rice|gb157.2|BE039784 94.7 blastp1119 poppy|gb166|FE964530 poppy 2183 234 rice|gb157.2|BE039784 92.1blastp 1120 poppy|gb166|FE965652 poppy 2184 234 rice|gb157.2|BE03978491.4 blastp 1121 potato|gb157.2|AW906248 potato 2185 234rice|gb157.2|BE039784 92.7 blastp 1122 potato|gb157.2|BF459889 potato2186 234 rice|gb157.2|BE039784 92.1 blastp 1123 potato|gb157.2|BG350431potato 2187 234 rice|gb157.2|BE039784 92.1 blastp 1124potato|gb157.2|BG351012 potato 2188 234 rice|gb157.2|BE039784 92.1blastp 1125 potato|gb157.2|BG351586 potato 2189 234rice|gb157.2|BE039784 92.05 tblastn 1126 prunus|gb167|BQ641170 prunus2190 234 rice|gb157.2|BE039784 92.7 blastp 1127 prunus|gb167|BU040848prunus 2191 234 rice|gb157.2|BE039784 93.4 blastp 1128pseudoroegneria|gb167|FF343278 pseudoroegneria 2192 234rice|gb157.2|BE039784 97.4 blastp 1129 pseudoroegneria|gb167|FF349878pseudoroegneria 2193 234 rice|gb157.2|BE039784 96 blastp 1130radish|gb164|EV527917 radish 2194 234 rice|gb157.2|BE039784 92.7 blastp1131 radish|gb164|EV528399 radish 2195 234 rice|gb157.2|BE039784 92.1blastp 1132 radish|gb164|EV535656 radish 2196 234 rice|gb157.2|BE03978492.7 blastp 1133 radish|gb164|EV535984 radish 2197 234rice|gb157.2|BE039784 92.7 blastp 1134 radish|gb164|EV538012 radish 2198234 rice|gb157.2|BE039784 92.7 blastp 1135 radish|gb164|EV543948 radish2199 234 rice|gb157.2|BE039784 92.7 blastp 1136 radish|gb164|EV544942radish 2200 234 rice|gb157.2|BE039784 92.7 blastp 1137radish|gb164|EV545164 radish 2201 234 rice|gb157.2|BE039784 92.7 blastp1138 radish|gb164|EV565378 radish 2202 234 rice|gb157.2|BE039784 92.05tblastn 1139 radish|gb164|EV565564 radish 2203 234 rice|gb157.2|BE03978492.7 blastp 1140 radish|gb164|EV565962 radish 2204 234rice|gb157.2|BE039784 92.7 blastp 1141 radish|gb164|EV569172 radish 2205234 rice|gb157.2|BE039784 92.7 blastp 1142 radish|gb164|EV571678 radish2206 234 rice|gb157.2|BE039784 92.7 blastp 1143 radish|gb164|EW714068radish 2207 234 rice|gb157.2|BE039784 92.7 blastp 1144radish|gb164|EW715107 radish 2208 234 rice|gb157.2|BE039784 92.7 blastp1145 radish|gb164|EW715768 radish 2209 234 rice|gb157.2|BE039784 92.7blastp 1146 radish|gb164|EX755320 radish 2210 234 rice|gb157.2|BE03978492.1 blastp 1147 radish|gb164|EX762413 radish 2211 234rice|gb157.2|BE039784 92.7 blastp 1148 radish|gb164|EX762524 radish 2212234 rice|gb157.2|BE039784 92.1 blastp 1149 radish|gb164|EX762893 radish2213 234 rice|gb157.2|BE039784 92.7 blastp 1150 radish|gb164|EY902515radish 2214 234 rice|gb157.2|BE039784 92.1 blastp 1151radish|gb164|EY916898 radish 2215 234 rice|gb157.2|BE039784 92.7 blastp1152 radish|gb164|T25179 radish 2216 234 rice|gb157.2|BE039784 92.7blastp 1153 rice|gb170|OS02G06700 rice 2217 234 rice|gb157.2|BE039784 98blastp 1154 rose|gb157.2|EC586094 rose 2218 234 rice|gb157.2|BE03978492.1 blastp 1155 rye|gb164|BE494213 rye 2219 234 rice|gb157.2|BE03978496.69 tblastn 1156 safflower|gb162|EL401182 safflower 2220 234rice|gb157.2|BE039784 90.7 blastp 1157 safflower|gb162|EL403588safflower 2221 234 rice|gb157.2|BE039784 90.1 blastp 1158safflower|gb162|EL408982 safflower 2222 234 rice|gb157.2|BE039784 86.09tblastn 1159 senecio|gb170|DY663041 senecio 2223 234rice|gb157.2|BE039784 88.7 blastp 1160 sorghum|gb161.crp|AW120027sorghum 2224 234 rice|gb157.2|BE039784 98.7 blastp 1161sorghum|gb161.crp|BE238630 sorghum 2225 234 rice|gb157.2|BE039784 98.7blastp 1162 sorghum|gb161.crp|BE367365 sorghum 2226 234rice|gb157.2|BE039784 98.7 blastp 1163 soybean|gb168|AI967817 soybean2227 234 rice|gb157.2|BE039784 92.1 blastp 1164 soybean|gb168|AJ388676soybean 2228 234 rice|gb157.2|BE039784 92.1 blastp 1165soybean|gb168|AW349445 soybean 2229 234 rice|gb157.2|BE039784 91.4blastp 1166 spikemoss|gb165|DN837720 spikemoss 2230 234rice|gb157.2|BE039784 87.4 blastp 1167 spikemoss|gb165|FE450939spikemoss 2231 234 rice|gb157.2|BE039784 87.4 blastp 1168spruce|gb162|CO216116 spruce 2232 234 rice|gb157.2|BE039784 92.1 blastp1169 spruce|gb162|CO227952 spruce 2233 234 rice|gb157.2|BE039784 92.1blastp 1170 spruce|gb162|DR449297 spruce 2234 234 rice|gb157.2|BE03978484.8 blastp 1171 spruce|gb162|DR449808 spruce 2235 234rice|gb157.2|BE039784 82.1 blastp 1172 spruce|gb162|DR474303 spruce 2236234 rice|gb157.2|BE039784 80.8 blastp 1173 spruce|gb162|DR534167 spruce2237 234 rice|gb157.2|BE039784 82.1 blastp 1174 spruce|gb162|DR579185spruce 2238 234 rice|gb157.2|BE039784 80.8 blastp 1175spurge|gb161|BE095303 spurge 2239 234 rice|gb157.2|BE039784 92.72tblastn 1176 spurge|gb161|DV124297 spurge 2240 234 rice|gb157.2|BE03978491.4 blastp 1177 strawberry|gb164|CO380977 strawberry 2241 234rice|gb157.2|BE039784 92.1 blastp 1178 strawberry|gb164|CO817246strawberry 2242 234 rice|gb157.2|BE039784 93.4 blastp 1179strawberry|gb164|EX670929 strawberry 2243 234 rice|gb157.2|BE039784 85.5blastp 1180 sugarcane|gb157.3|BQ529920 sugarcane 2244 234rice|gb157.2|BE039784 97.35 tblastn 1181 sugarcane|gb157.3|BQ533000sugarcane 2245 234 rice|gb157.2|BE039784 98.7 blastp 1182sugarcane|gb157.3|CA076561 sugarcane 2246 234 rice|gb157.2|BE039784 97.4blastp 1183 sugarcane|gb157.3|CA102375 sugarcane 2247 234rice|gb157.2|BE039784 96.03 tblastn 1184 sugarcane|gb157.3|CA123229sugarcane 2248 234 rice|gb157.2|BE039784 96 blastp 1185sugarcane|gb157.3|CA137141 sugarcane 2249 234 rice|gb157.2|BE039784 98.7blastp 1186 sugarcane|gb157.3|CA230074 sugarcane 2250 234rice|gb157.2|BE039784 92.72 tblastn 1187 sunflower|gb162|AJ318263sunflower 2251 234 rice|gb157.2|BE039784 90.1 blastp 1188sunflower|gb162|CD848093 sunflower 2252 234 rice|gb157.2|BE039784 91.4blastp 1189 sunflower|gb162|CD848805 sunflower 2253 234rice|gb157.2|BE039784 90.1 blastp 1190 sunflower|gb162|EL430967sunflower 2254 234 rice|gb157.2|BE039784 82.8 blastp 1191switchgrass|gb167|DN149917 switchgrass 2255 234 rice|gb157.2|BE03978496.7 blastp 1192 switchgrass|gb167|DN150990 switchgrass 2256 234rice|gb157.2|BE039784 98.7 blastp 1193 switchgrass|gb167|FE599497switchgrass 2257 234 rice|gb157.2|BE039784 96 blastp 1194switchgrass|gb167|FE608350 switchgrass 2258 234 rice|gb157.2|BE03978496.7 blastp 1195 switchgrass|gb167|FE625398 switchgrass 2259 234rice|gb157.2|BE039784 80.13 tblastn 1196 switchgrass|gb167|FE627660switchgrass 2260 234 rice|gb157.2|BE039784 98.7 blastp 1197switchgrass|gb167|FE634044 switchgrass 2261 234 rice|gb157.2|BE039784 98blastp 1198 switchgrass|gb167|FE637032 switchgrass 2262 234rice|gb157.2|BE039784 97.4 blastp 1199 switchgrass|gb167|FL948269switchgrass 2263 234 rice|gb157.2|BE039784 82.12 tblastn 1200switchgrass|gb167|GD043911 switchgrass 2264 234 rice|gb157.2|BE03978480.13 tblastn 1201 tamarix|gb166|EG966933 tamarix 2265 234rice|gb157.2|BE039784 93.4 blastp 1202 tamarix|gb166|EG972900 tamarix2266 234 rice|gb157.2|BE039784 82.8 blastp 1203thellungiella|gb167|BY818453 thellungiella 2267 234rice|gb157.2|BE039784 94 blastp 1204 thellungiella|gb167|DN775374thellungiella 2268 234 rice|gb157.2|BE039784 94 blastp 1205tobacco|gb162|AM816373 tobacco 2269 234 rice|gb157.2|BE039784 81.5blastp 1206 tobacco|gb162|CN498843 tobacco 2270 234rice|gb157.2|BE039784 82.2 blastp 1207 tobacco|gb162|CV019114 tobacco2271 234 rice|gb157.2|BE039784 91.4 blastp 1208 tobacco|gb162|CV020233tobacco 2272 234 rice|gb157.2|BE039784 91.4 blastp 1209tobacco|gb162|CV021807 tobacco 2273 234 rice|gb157.2|BE039784 92.7blastp 1210 tobacco|gb162|NTU66262 tobacco 2274 234rice|gb157.2|BE039784 90.7 blastp 1211 tomato|gb164|BG123159 tomato 2275234 rice|gb157.2|BE039784 92.1 blastp 1212 tomato|gb164|BG123562 tomato2276 234 rice|gb157.2|BE039784 92.7 blastp 1213 tomato|gb164|U21078tomato 2277 234 rice|gb157.2|BE039784 92.7 blastp 1214triphysaria|gb164|BM357412 triphysaria 2278 234 rice|gb157.2|BE03978492.7 blastp 1215 triphysaria|gb164|EX988766 triphysaria 2279 234rice|gb157.2|BE039784 91.4 blastp 1216 triphysaria|gb164|EX990185triphysaria 2280 234 rice|gb157.2|BE039784 91.4 blastp 1217triphysaria|gb164|EX992752 triphysaria 2281 234 rice|gb157.2|BE03978491.4 blastp 1218 volvox|gb162|AW676072 volvox 2282 234rice|gb157.2|BE039784 85 blastp 1219 walnuts|gb166|CV197623 walnuts 2283234 rice|gb157.2|BE039784 92.1 blastp 1220 walnuts|gb166|EL891118walnuts 2284 234 rice|gb157.2|BE039784 94.7 blastp 1221wheat|gb164|AL827137 wheat 2285 234 rice|gb157.2|BE039784 97.4 blastp1222 wheat|gb164|BE398647 wheat 2286 234 rice|gb157.2|BE039784 96 blastp1223 wheat|gb164|BE398957 wheat 2287 234 rice|gb157.2|BE039784 97.4blastp 1224 wheat|gb164|BE405321 wheat 2288 234 rice|gb157.2|BE039784 96blastp 1225 wheat|gb164|BE406789 wheat 2289 234 rice|gb157.2|BE03978495.4 blastp 1226 wheat|gb164|BJ240969 wheat 2290 234rice|gb157.2|BE039784 80.79 tblastn 1227 wheat|gb164|BM135152 wheat 2291234 rice|gb157.2|BE039784 82.9 blastp 1228 wheat|gb164|CA616908 wheat2292 234 rice|gb157.2|BE039784 94.7 tblastn 1229 wheat|gb164|CJ652504wheat 2293 234 rice|gb157.2|BE039784 93.4 blastp 1230wheat|gb164|DN829631 wheat 2294 234 rice|gb157.2|BE039784 80.92 tblastn1231 zamia|gb166|DY032098 zamia 2295 234 rice|gb157.2|BE039784 91.4blastp 1232 barley|gb157.3|BE412461 barley 2296 236 maize|gb164|AI61926987.9 blastp 1233 brachypodium|gb169|BE404324 brachypodium 2297 236maize|gb164|AI619269 86.4 blastp 1234 cenchrus|gb166|EB653779 cenchrus2298 236 maize|gb164|AI619269 94.8 blastp 1235 fescue|gb161|DT696747fescue 2299 236 maize|gb164|AI619269 85.6 blastp 1236leymus|gb166|EG375640 leymus 2300 236 maize|gb164|AI619269 88.8 blastp1237 lovegrass|gb167|EH189611 lovegrass 2301 236 maize|gb164|AI619269 92blastp 1238 maize|gb170|AI944307 maize 2302 236 maize|gb164|AI61926994.4 blastp 1239 oat|gb164|BE439172 oat 2303 236 maize|gb164|AI61926985.58 tblastn 1240 pseudoroegneria|gb167|FF354244 pseudoroegneria 2304236 maize|gb164|AI619269 88.4 blastp 1241 rice|gb170|OS02G53790 rice2305 236 maize|gb164|AI619269 89.3 blastp 1242 rice|gb170|OS07G43170rice 2306 236 maize|gb164|AI619269 90.1 blastp 1243sorghum|gb161.crp|AW011679 sorghum 2307 236 maize|gb164|AI619269 95.8blastp 1244 sorghum|gb161.crp|CD231888 sorghum 2308 236maize|gb164|AI619269 90.6 blastp 1245 sugarcane|gb157.3|CA072943sugarcane 2309 236 maize|gb164|AI619269 95.3 blastp 1246sugarcane|gb157.3|CA090072 sugarcane 2310 236 maize|gb164|AI619269 95.8blastp 1247 switchgrass|gb167|DN145249 switchgrass 2311 236maize|gb164|AI619269 92.5 blastp 1248 switchgrass|gb167|FE626130switchgrass 2312 236 maize|gb164|AI619269 93.4 blastp 1249arabidopsis|gb165|AT3G01300 arabidopsis 2313 237arabidopsis|gb157.2|AT5G15080 83.3 blastp 1250 b_rapa|gb162|CA992096b_rapa 2314 237 arabidopsis|gb157.2|AT5G15080 82.5 blastp 1251canola|gb161|EE473973 canola 2315 237 arabidopsis|gb157.2|AT5G15080 82.3blastp 1252 arabidopsis|gb165|AT1G60690 arabidopsis 2316 239arabidopsis|gb165|AT1G60680 84.1 blastp 1253 arabidopsis|gb165|AT1G60710arabidopsis 2317 239 arabidopsis|gb165|AT1G60680 83.2 blastp 1254arabidopsis|gb165|AT1G60730 arabidopsis 2318 239arabidopsis|gb165|AT1G60680 84.44 tblastn 1255 b_rapa|gb162|ES935213b_rapa 2319 239 arabidopsis|gb165|AT1G60680 82.1 blastp 1256canola|gb161|CD815566 canola 2320 239 arabidopsis|gb165|AT1G60680 80.6blastp 1257 canola|gb161|CD819004 canola 2321 239arabidopsis|gb165|AT1G60680 81.5 blastp 1258 canola|gb161|DY003163canola 2322 239 arabidopsis|gb165|AT1G60680 81.8 blastp 1259radish|gb164|EV524749 radish 2323 239 arabidopsis|gb165|AT1G60680 81.6blastp 1260 radish|gb164|EV544729 radish 2324 239arabidopsis|gb165|AT1G60680 81.8 blastp 1261 b_rapa|gb162|EX018587b_rapa 2325 242 arabidopsis|gb165|AT1G43910 85.3 blastp 1262canola|gb161|EE452442 canola 2326 242 arabidopsis|gb165|AT1G43910 91.5blastp 1263 radish|gb164|EX749875 radish 2327 243arabidopsis|gb157.2|AT1G47530 91.94 tblastn 1264arabidopsis|gb165|AT4G30940 arabidopsis 2328 244arabidopsis|gb157.2|AT2G24240 88.3 blastp 1265 castorbean|gb160|EG675736castorbean 2329 244 arabidopsis|gb157.2|AT2G24240 83.6 blastp 1266cotton|gb164|CO495384 cotton 2330 244 arabidopsis|gb157.2|AT2G24240 83.3blastp 1267 nicotiana_benthamiana|gb162|CK280239 nicotiana_benthamiana2331 244 arabidopsis|gb157.2|AT2G24240 80.1 blastp 1268poplar|gb170|CA822859 poplar 2332 244 arabidopsis|gb157.2|AT2G24240 81.2blastp 1269 poplar|gb170|CV237453 poplar 2333 244arabidopsis|gb157.2|AT2G24240 81.8 blastp 1270 potato|gb157.2|CK243505potato 2334 244 arabidopsis|gb157.2|AT2G24240 83.1 blastp 1271soybean|gb168|AW586330 soybean 2335 244 arabidopsis|gb157.2|AT2G2424080.3 blastp 1272 soybean|gb168|BP073481 soybean 2336 244arabidopsis|gb157.2|AT2G24240 80.4 blastp 1273 barley|gb157.3|AL502083barley 2337 246 rice|gb157.2|BI807603 89.93 tblastn 1274brachypodium|gb169|BE471061 brachypodium 2338 246 rice|gb157.2|BI80760390.3 blastp 1275 maize|gb170|AW066842 maize 2339 246rice|gb157.2|BI807603 88.1 blastp 1276 maize|gb170|CF021466 maize 2340246 rice|gb157.2|BI807603 81 blastp 1277 maize|gb170|LLCD975615 maize2341 246 rice|gb157.2|BI807603 88.8 blastp 1278 maize|gb170|T12700 maize2342 246 rice|gb157.2|BI807603 91.8 blastp 1279sorghum|gb161.crp|AW066842 sorghum 2343 246 rice|gb157.2|BI807603 92.9blastp 1280 sorghum|gb161.crp|AW747438 sorghum 2344 246rice|gb157.2|BI807603 82.1 blastp 1281 sugarcane|gb157.3|BU925651sugarcane 2345 246 rice|gb157.2|BI807603 88.27 tblastn 1282switchgrass|gb167|FL745129 switchgrass 2346 246 rice|gb157.2|BI80760391.8 blastp 1283 wheat|gb164|BE471061 wheat 2347 246rice|gb157.2|BI807603 89.7 blastp 1284 rice|gb170|OS01G09340 rice 2348247 rice|gb157.2|AU068829 86.29 tblastn 1285 brachypodium|gb169|AV835247brachypodium 2349 248 rice|gb157.2|AA752451 86.7 blastp 1286maize|gb170|BG835950 maize 2350 248 rice|gb157.2|AA752451 80.7 blastp1287 sorghum|gb161.crp|BE598733 sorghum 2351 248 rice|gb157.2|AA75245181.4 blastp 1288 sugarcane|gb157.3|CA101548 sugarcane 2352 248rice|gb157.2|AA752451 84.5 blastp 1289 switchgrass|gb167|FE639520switchgrass 2353 248 rice|gb157.2|AA752451 88.9 blastp 1290barley|gb157.3|AL511842 barley 2354 250 wheat|gb164|BE401454 98.4 blastp1291 brachypodium|gb169|BE488258 brachypodium 2355 250wheat|gb164|BE401454 93.4 blastp 1292 fescue|gb161|DT699211 fescue 2356250 wheat|gb164|BE401454 90.2 blastp 1293 leymus|gb166|CD808752 leymus2357 250 wheat|gb164|BE401454 97.6 blastp 1294pseudoroegneria|gb167|FF347865 pseudoroegneria 2358 250wheat|gb164|BE401454 99.2 blastp 1295 rice|gb170|OS08G45190 rice 2359250 wheat|gb164|BE401454 81.9 blastp 1296 sorghum|gb161.crp|AW287236sorghum 2360 250 wheat|gb164|BE401454 83.7 blastp 1297wheat|gb164|BE488191 wheat 2361 250 wheat|gb164|BE401454 99.2 blastp1298 arabidopsis|gb165|AT1G70830 arabidopsis 2362 251arabidopsis|gb165|AT1G70850 85.4 blastp 1299arabidopsis|gb165|AT1G70830T4 arabidopsis 2363 251arabidopsis|gb165|AT1G70850 88.24 tblastn 1300 barley|gb157.3|BE420890barley 2364 254 sorghum|gb161.xeno|T18303 81.5 blastp 1301brachypodium|gb169|BE401954 brachypodium 2365 254sorghum|gb161.xeno|T18303 83 blastp 1302 cenchrus|gb166|EB656949cenchrus 2366 254 sorghum|gb161.xeno|T18303 89.1 blastp 1303fescue|gb161|DT686385 fescue 2367 254 sorghum|gb161.xeno|T18303 80.1blastp 1304 leymus|gb166|CN466500 leymus 2368 254sorghum|gb161.xeno|T18303 82.2 blastp 1305 maize|gb170|AF093538 maize2369 254 sorghum|gb161.xeno|T18303 91.3 blastp 1306 maize|gb170|T18303maize 2370 254 sorghum|gb161.xeno|T18303 94.9 blastp 1307pseudoroegneria|gb167|FF348742 pseudoroegneria 2371 254sorghum|gb161.xeno|T18303 81.2 blastp 1308 rice|gb170|OS09G19734T3 rice2372 254 sorghum|gb161.xeno|T18303 83.7 blastp 1309sugarcane|gb157.3|BQ533149 sugarcane 2373 254 sorghum|gb161.xeno|T1830397.5 blastp 1310 switchgrass|gb167|DN141290 switchgrass 2374 254sorghum|gb161.xeno|T18303 89.1 blastp 1311 switchgrass|gb167|DN141310switchgrass 2375 254 sorghum|gb161.xeno|T18303 89.5 blastp 1312wheat|gb164|BE406144 wheat 2376 254 sorghum|gb161.xeno|T18303 81.5blastp 1313 wheat|gb164|BF200548 wheat 2377 254sorghum|gb161.xeno|T18303 81.9 blastp 1314 wheat|gb164|X77733 wheat 2378254 sorghum|gb161.xeno|T18303 81.2 blastp 1315 maize|gb170|CD936650maize 2379 269 sorghum|gb161.xeno|AW923465 89.12 tblastn 1315maize|gb170|CD936650 maize 2379 256 sorghum|gb161.crp|AW923545 88.85tblastn 1316 aquilegia|gb157.3|DR915439 aquilegia 2380 257arabidopsis|gb165|AT1G71900 81.1 blastp 1317 arabidopsis|gb165|AT1G34470arabidopsis 2381 257 arabidopsis|gb165|AT1G71900 80.11 tblastn 1318castorbean|gb160|EE258327 castorbean 2382 257arabidopsis|gb165|AT1G71900 81 blastp 1319castorbean|gb160|MDL29728M000834 castorbean 2383 257arabidopsis|gb165|AT1G71900 81.56 tblastn 1320 grape|gb160|CB035795grape 2384 257 arabidopsis|gb165|AT1G71900 82.4 blastp 1321radish|gb164|EW714634 radish 2385 257 arabidopsis|gb165|AT1G71900 89.74tblastn 1322 maize|gb170|CF021816 maize 2386 259sorghum|gb161.xeno|AW672541 88.3 blastp 1323 maize|gb170|CO527882 maize2387 259 sorghum|gb161.xeno|AW672541 85 blastp 1324switchgrass|gb167|FE626524 switchgrass 2388 259sorghum|gb161.xeno|AW672541 85.4 blastp 1325 sugarcane|gb157.3|CA069240sugarcane 2389 262 sorghum|gb161.xeno|BE123399 89.08 tblastn 1326sugarcane|gb157.3|CA078694 sugarcane 2390 262sorghum|gb161.xeno|BE123399 88.4 blastp 1327 maize|gb170|AI901557 maize2391 263 sorghum|gb161.xeno|AI901557 86.2 blastp 1328sorghum|gb161.crp|AW286491 sorghum 2392 263 sorghum|gb161.xeno|AI90155798.5 blastp 1329 sugarcane|gb157.3|CA068682 sugarcane 2393 263sorghum|gb161.xeno|AI901557 93.9 blastp 1330 switchgrass|gb167|DN146139switchgrass 2394 263 sorghum|gb161.xeno|AI901557 83.6 blastp 1331switchgrass|gb167|FE604486 switchgrass 2395 263sorghum|gb161.xeno|AI901557 82.7 blastp 1332 maize|gb170|CD945482 maize2396 265 maize|gb164|AI974922 81.53 tblastn 1333sorghum|gb161.crp|BE599314 sorghum 2397 265 maize|gb164|AI974922 80.49tblastn

Table 21: Provided are the homologous polypeptides (polypep.) andpolynucleotides (polynucl.) of the genes and polypeptides identified inTable 20, which are capable of increase nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent, abiotic stress tolerance and/or water use efficiency of aplant. Homology was calculated as % of identity over the alignedsequences. The query sequences were polypeptide sequences SEQ IDNOs:138-269 and the subject sequences are polypeptide sequences ornucleotide sequences which were dynamically translated in all sixreading frames identified in the database based on greater than 80%identity to the query polypeptide sequences.

Example 3 Gene Cloning and Generation of Binary Vectors for Expressionin Plants

Cloning Strategy

Genes presented in Examples 1 and 2 above were cloned into binaryvectors for the generation of transgenic plants. For cloning, thefull-length open reading frames (ORF) were first identified. ESTclusters and in some cases mRNA sequences were analyzed to identify theentire open reading frame of each gene, by comparing the results ofseveral translation algorithms to known proteins from other plantspecies.

In order to clone the full-length cDNAs, Reverse Transcription followedby PCR (RT-PCR) was performed on total RNA extracted from leaves, roots,fibers or other plant tissues, growing under either normal, nutrientdeficient or other abiotic stress conditions. Total RNA extraction,production of cDNA and PCR amplification was performed using standardprotocols described elsewhere (Sambrook J., E. F. Fritsch, and T.Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd Ed. ColdSpring Harbor Laboratory Press, New York.), which are well known tothose skilled in the art. PCR products were purified using PCRpurification kit (Qiagen) and sequencing of the amplified PCR productswas performed, using ABI 377 sequencer (Applied Biosystems). In case ofnone or weak PCR product bands were visible on Ethidium Bromide—stained1% agarose gels, 0.1-1 μL of the PCR product was used as a DNA template,and PCR amplification was effected using either the same or new set ofprimers, designed internally to the first set of primers. In such cases,the set of primers which is expected to produce the longer PCR productis designated External primers set (EF and ER for External-Forward andExternal-Reverse, respectively), and the set of primers which expectedto produce the shorter PCR product is designated Nested primers set (NFand NR for Nested-Forward and Nested-Reverse, respectively), asillustrated in Table 22 below. Cloning of the cotton genes CT75, CT7,CT76, CT71, CT74, CT11, CT20, CT81, CT22, CT82, CT3, CT40, CT1, CT6,CT27 and CT2 was performed using only one set of primers, as detailed inWO Publication No: WO2005/121364.

To facilitate cloning of the cDNAs, a 7-12 bp extension was added to the5′ prime end of most of the primers. The primer extension includes anendonuclease restriction site (Table 22). The restriction sites wereselected using two parameters: (a). The site does not exist in the cDNAsequence; and (b). The restriction sites in the forward and reverseprimers are designed such that the digested cDNA is inserted in thesense formation into the binary vector utilized for transformation.Table 22, hereinbelow, provides the primers designation, restrictionendonuclease sites added for the subsequent cloning, and sequences ofeach primer used for the amplification of the genes of the someembodiments of the invention.

TABLE 22 PCR primers used for cloning and for screening positive clonesSEQ SEQ ID ID Gene ID Enz. Primers for cloning NO: Primers for screeningNO: Plas. NUE227 SalI, NUE22 TTAGTCGACAG 2564 101-F GCTATGACCAT 2860pGXN 7_EF_ AGAAGAGGCAA GATTACGCC SalI GAACAACTAG XbaI NUE22 TATCTAGACGAT2565 7_ER_ CGGTGTCCACTG XbaI TACAG NUE22 TTAGTCGACACT 2566 7_NF_AGCTGCATGGC SalI AATGG NUE22 TTAGTCGACACT 2567 NUE22 TATCTAGATTA 28617_NR_ AGCTGCATGGC 7_NR_ ACGCGTTGATC XbaI AATGG XbaI GATCAGC NUE233 SalI,NUE23 TTAGTCGACCTC 2568 pKsJ or 3_EF_ GAAATCCTTCCC Topo SalI AAGAC XbaINUE23 TATCTAGAGTCA 2569 3_ER_ CAGAATAGTAC XbaI ACGTACACAAC NUE23TTAGTCGACCGC 2570 3_NF_ ACGCTTCTCCAT SalI TTC NUE23 TATCTAGATCAA 25713_NR_ ACTAAGTACTCC XbaI AGTAACAAC NUE237 SalI, NUE23 AAAGTCGACCC 257235S_1F GGAGAGGACA 2862 pGXN 7_EF_ TCTCTCTCGTTT GGCTTCTTGAG SalI CGATTCCXbaI NUE23 ATTCTAGATCAA 2573 NUE23 ACTCTAGAACT 2863 7_ER_ CCACATAGCCT7_NR_ CTATTAACAAT XbaI AGAGCAC XbaI GCACGGAG NUE23 AAAGTCGACAG 25747_NF_ ATTCGATCCAAC SalI CAAACC NUE23 ACTCTAGAACTC 2575 7_NR_TATTAACAATGC XbaI ACGGAG NUE241 SalI, NUE24 AAAGTCGACAA 2576 35S_1FGGAGAGGACA 2864 pGXN 1_EF_ TTCTTCTTTGTT GGCTTCTTGAG SalI TGCTTGC XbaINUE24 ATTCTAGATAA 2577 NUE24 ATTCTAGATCA 2865 1_ER_ ATGCTGATATA 1_NR_CAATAGAAAC XbaI GGACAAAGC XbaI ATCCTCCCTC NUE24 AAAGTCGACGA 2578 1_NF_AGAAAACCCAC SalI AAAACCAG NUE24 ATTCTAGATCAC 2579 1_NR_ AATAGAAACAT XbaICCTCCCTC NUE242 XbaI, NUE24 TATCTAGAGAG 2580 p35S_F GGACAGGCTT 2866 pGXN2_EF_ AAGAGAGAGAC 2 CTTGAGATCCT XbaI TTTGAAGATG SacI NUE24 TGAGCTCTTAAG2581 NUE24 TGAGCTCTTAT 2867 2_ER_ AGTAGACACAA 2_NR_ TAGGAAGCAA SacICTCCTGCG SacI CTTCAAGAAAT G SalI, NUE24 TTAGTCGACTGA 2582 2_NF_AGATGGAAGCA SalI AACTCTAAC SacI NUE24 TGAGCTCTTATT 2583 2_NR_AGGAAGCAACT SacI TCAAGAAATG NUE255 EcoR NUE25 ATGATATCCCTC 2584 p35S_FGGACAGGCTT 2868 Topo V 5_EF_ CAACCTCTCTCC 2 CTTGAGATCCT EcoRV CAAC NUE25TAGATATCGATT 2585 NUE25 TAGATATCTCA 2869 5_ER_ GCTTCTTGTACT 5_NR_TCATTTGATCA EcoRV CTGATCATC EcoRV GCTTTAGCG NUE25 ATGATATCCAA 2586 5_NF_GAATTAAGGTG EcoRV TAGCAACC NUE25 TAGATATCTCAT 2587 5_NR_ CATTTGATCAGCEcoRV TTTAGCG NUE269 SalI, NUE26 TATGTCGACAC 2588 35S_1F GGAGAGGACA 2870pGXN 9_NF_ AAGGAAATGAT GGCTTCTTGAG SalI GGCTATTG XbaI NUE26 TATCTAGACACC2589 NUE26 TATCTAGACAC 2871 9_NR_ ACAACATGATA 9_NR_ CACAACATGA XbaIGCTTTTG XbaI TAGCTTTTG NUE521 Sal, NUE52 AAGGTCGACCT 2590 p35S_FGGACAGGCTT 2872 pGXN 1 NF GGGAGCTAGCT 2 CTTGAGATCCT Sal TTGGAG Xba NUE52ACTCTAGATCAC 2591 NUE52 CGTCTAGATCA 2873 1 ER ACCGATTCCAC 1 NRGATCGTGTTGA Xba ACATAAC Xba GCACTTGAGC NUE52 AAGGTCGACCT 2592 1 NFGGGAGCTAGCT Sal TTGGAG NUE52 CGTCTAGATCA 2593 1 NR GATCGTGTTGA XbaGCACTTGAGC NUE554 SmaI, NUE55 TCCCGGGCTCCG 2594 35S_1F GGAGAGGACA 2874pGXN 4_EF_ TCTCTAGGGTTT GGCTTCTTGAG SmaI GAG SacI NUE55 TGAGCTCTCAGT2595 NUE55 TGAGCTCTCAG 2875 4_ER_ GATTGGAACTCT 4_ER_ TGATTGGAACT SacIAGATCTTG SacI CTAGATCTTG NUE562 XbaI, NUE56 TATCTAGACTTG 2596 35S_1FGGAGAGGACA 2876 pGXN 2_EF_ AGCTAGGGTTTT GGCTTCTTGAG XbaI ATCGC SacINUE56 TGAGCTCTTAAT 2597 NUE56 TGAGCTCTTAT 2877 2_ER_ GCAGACGGTAA 2_NR_GAAGATTACA SacI CATCTAGG SacI GCCTCCTACC NUE56 TATCTAGAAAC 2598 2_NF_AATGTCCGGGA XbaI GGAAGAAGAC NUE56 TGAGCTCTTATG 2599 2_NR_ AAGATTACAGCSacI CTCCTACC NUE567 Sal, NUE56 AGAGTCGACGT 2600 35S_1F GGAGAGGACA 2878pGXN 7_EF_ GACATAAAATC GGCTTCTTGAG Sal CATGGCTG Xba NUE56 TATCTAGATCAG2601 NUE56 ACCTCTAGATC 2879 7_ER_ CTTACACAAGC 7 NR ATTAAGTGGCT XbaCCTTAGCA Xba TTCCAGGAAG NUE56 GAGGTCGACAA 2602 7_NF TCCATGGCTGA SalAGCTTG NUE56 ACCTCTAGATCA 2603 7 NR TTAAGTGGCTTT Xba CCAGGAAG NUE568Sal, NUE56 AGAGTCGACCG 2604 35S_1F GGAGAGGACA 2880 pGXN 8 EF CAACGGAAAAC GGCTTCTTGAG Sal AAATC Xba NUE56 TATCTAGAAGA 2605 NUE56TATCTAGATCA 2881 8 ER TAGGCTTATCTC 8 NR TGTTCACTGAG Xba AATGGCT XbaTAACGATACTA ACAG NUE56 TAGGTCGACAC 2606 8 NF AAATCCGCCAA Sal TGGAAGNUE56 TATCTAGATCAT 2607 8 NR GTTCACTGAGTA Xba ACGATACTAAC AG NUE573 Sal,NUE TAGGTCGACGA 2608 35S_1F GGAGAGGACA 2882 pKsJ 573 EF GAGAAATCCATGGCTTCTTGAG Sal GGAGACG Sac NUE57 CGAGCTCAATTT 2609 NUE57 CGAGCTCTCAG2883 3_ER CAGTACAGGAT 3 NR TACAGGATTTA Sac TTAAACC Sac AACCAAGACA Sma,NUE57 ACCCGGGAGAC 2610 3_NF GATGACGATGA Sma AGGTTG Sac NUE57CGAGCTCTCAGT 2611 3 NR ACAGGATTTAA Sac ACCAAGACA NUE575 EcoR NUE57AAGATATCCCA 2612 NUE57 AAGATATCCC 2884 pKsJ V 5_NF_E AACACCAAACC 5_NF_EAAACACCAAA coRV CTCG coRV CCCTCG NUE57 TAGATATCTCAT 2613 101_RAAGTTGGGTA 2885 5_NR_ CATATTCCTAGC ACGCCAGGGT EcoRV TTATCAACCTC NUE585SalI, NUE58 AAAGTCGACCG 2614 35S_1F GGAGAGGACA 2886 pGXN 5_EF_ATTTCTGCTTCG GGCTTCTTGAG SalI ATCTCTAC XbaI NUE58 ATTCTAGACCTT 2615NUE58 ATTCTAGATTA 2887 5_ER_ CTTCGATCTTCT 5_NR_ GTTTGCAGTTA XbaI TGAACCXbaI TCGCAGTGG NUE58 AAAGTCGACGT 2616 5_NF_ CTGGGTCGAAG SalI TTAAATAGGNUE58 ATTCTAGATTAG 2617 5_NR_ TTTGCAGTTATC XbaI GCAGTGG NUE587 SalI,NUE58 AAAGTCGACGT 2618 35S_1F GGAGAGGACA 2888 pGXN 7_EF_ TCCATTGGAGGGGCTTCTTGAG SalI AGAATCG XbaI NUE58 ATTCTAGATTCA 2619 NUE58 ATTCTAGATTA2889 7_ER_ AAAGGAAAATG 7_NR_ TTTCAAACATG XbaI GAGAGG XbaI AAATGAGTTG CNUE58 AAAGTCGACAA 2620 7_NF_ AGGCTTGGAAA SalI GGAAGG NUE58 ATTCTAGATTAT2621 7_NR_ TTCAAACATGA XbaI AATGAGTTGC NUE528 Sac NUE52 AGAGCTCAACC 2622101F GCTATGACCAT 2890 pGXN 8_EF_ CTAACGTTTCGA GATTACGCC Sac TCG SalI,NUE52 TGAGCTCTTCCA 2623 NUE52 TGAGCTCTGGC 2891 8_ER_ GAAGTAGCATC 8_NR_CTTCACCCTCT Sac TTTCG Sac ATATCTC NUE52 AATGTCGACGA 2624 8_NF_AGCGTCTGAGC SalI CAGTCC NUE52 TGAGCTCTGGCC 2625 8_NR_ TTCACCCTCTAT SacATCTC NUE535 Sal, NUE53 ATTGTCGACGA 2626 101_F GCTATGACCAT 2892 pGXN5_NF_ GTATGCTTTCCG GATTACGCC Sal ATGGG XbaI NUE53 TTTCTAGACTAT 2627NUE53 TTTCTAGACTA 2893 5_NR_ GAATGAATCCG 5_NR_ TGAATGAATCC XbaITGACTCTTG XbaI GTGACTCTTG NUE538 Sal, NUE53 ATTGTCGACCAC 2628 NUE53ATTGTCGACCA 2894 pKSJ 8_EF_ GACCATTCTTCA 8_EF_ CGACCATTCTT Sal TTTTCCSal CATTTTCC Sma NUE53 TCCCGGGTTAG 2629 NOS R GCGGGACTCT 2895 8_ER_AACTGAGTCTG AATCATAAAA Sma AAAGGATGG ACC NUE548 Sal NUE54 AATGTCGACGT2630 101_F GCTATGACCAT 2896 pGXN 8_NF_ CCTAATACTATA GATTACGCC SalCTCGCAATCC Xba NUE54 AATCTAGATCA 2631 NUE54 AATCTAGATCA 2897 8_NR_ACCAACTAGTTT 8_NR_ ACCAACTAGTT Xba GCAGCTCCT Xba TGCAGCTCCT NUE537 Sal,NUE53 TAAGTCGACCA 2632 101_ER GAAACACCAT 2898 pGXN 7_NF_ AACAACATGTCCTTCGTTCTTG Sal TGCCTGTG Xba NUE53 ATTCTAGATTAA 2633 NUE53 TAAGTCGACC2899 7_NR_ CACATCGTTTGG 7_NF_ AAACAACATG Xba TGCATAGC Sal TCTGCCTGTGNUE551 Sal, NUE55 AATGTCGACGTT 2634 NUE55 GTCAAGCTGTG 2900 pGXN 1_NF_GATCAGTCAGC 1_seqF CTGTCTTCC Sal CCACTTC Xba NUE55 TATCTAGAGAC 2635101_ER GAAACACCAT 2901 1_ER_ ATAATCCATCA CTTCGTTCTTG Xba ACGGTTG NUE553Xba. NUE55 AATCTAGACTC 2636 NUE55 AATCTAGAGA 2902 pGXN 3_EF_ ACGAATCCACC3_NF_ CACGGACCGA Xba GATCAG Xba ACAGCTAG Sma NUE55 TCCCGGGACAC 2637NOS_R GCGGGACTCT 2903 3_ER_ ACATCATGGCT AATCATAAAA Sma GTTACAG ACC NUE55AATCTAGAGAC 2638 3_NF_ ACGGACCGAAC Xba AGCTAG NUE55 TCCCGGGCGAC 26393_NR_ TTCATATACAGA Sma CGGATG NUE511 Xba. NUE51 AATCTAGAGAT 2640 NUE51AATCTAGAGA 2904 pGXN 1_EF_ TAGGAGCAGGG 1_EF_ TTAGGAGCAG Xba ACCAATC XbaGGACCAATC Sac NUE51 TGAGCTCTTAGG 2641 101_ER GAAACACCAT 2905 1_NR_TACATGATGAC CTTCGTTCTTG Sac ATTTCAGCA NUE512 Xba. NUE51 AATCTAGACCT 2642NUE51 AATCTAGACCT 2906 pGXN 2_NF_ ATTGCTCATGAT 2_NF_ ATTGCTCATGA XbaGTTTGA Xba TGTTTGA Sac NUE51 TGAGCTCTTACA 2643 Nos_R GCGGGACTCT 29072_NR_ AAGGCAGGAAA AATCATAAAA Sac TACAGAAG ACC NUE542 XbaI, NUE54TATCTAGAAATT 2644 NUE54 GTACGTCTCCG 2908 pGXN 2_EF_ TAGCTCGTTGAT 2_seqFTCCGACAAC XbaI GATGG SacI NUE54 TGAGCTCCTAGT 2645 101_ER GAAACACCAT 29092_ER_ GTCCATGTCAAT CTTCGTTCTTG sacI GATGTC NUE54 TATCTAGATAGC 2646 2_NF_TCGTTGATGATG XbaI GAGG NUE54 TGAGCTCTTATC 2647 2_NR_ CATGTCAATGAT SacIGTCCATC NUE569 SalI NUE56 AAAGTCGACGC 2648 35S_1F GGAGAGGACA 2910 pGXN9_NF_ TACTGCTTCTTC GGCTTCTTGAG SalI TGTTCACC SacI NUE56 TGAGCTCTACTA2649 NUE56 GAGATGGAGC 2911 9_NR_ CCATAGAACTG 9_seqR CTTGTCATGA SacIAAGAAGAAGTC NUE244 SalI, NUE24 TTAGTCGACTAG 2650 35S_1F GGAGAGGACA 2912pGXN 4_NF_ ACTGATGGGAA GGCTTCTTGAG SalI GTGTTCC XbaI NUE24 TATCTAGACTAC2651 NUE24 TATCTAGACTA 2913 4_NR_ TACACGGATTG 4_NR_ CTACACGGATT XbaICCCAAAC XbaI GCCCAAAC NUE577 NUE57 AATCTAGAGTTT 2652 35S_1F GGAGAGGACA2914 TopoB 7_NF_ ATCTTGTTTTGG GGCTTCTTGAG XbaI GTTTGG NUE57 TCCCGGGGTGA2653 NUE57 TCCCGGGGTG 2915 7_NR_ AAGATCTCAGA 7_NR_ AAAGATCTCA SmaICCACCTC SmaI GACCACCTC NUE253 XbaI, NUE25 TATCTAGACTTC 2654 35S_1FGGAGAGGACA 2916 pKSJ 3_EF_ TTCCTCCATATC GGCTTCTTGAG XbaI ACACG SmaINUE25 TCCCGGGTCAC 2655 3_ER_ GTGGCATGCAT SmaI GATCTG NUE25 TATCTAGAAAC2656 NUE25 TCCCGGGTCAT 2917 3_NF_ AATGGATGGGG 3_NR_ CACTCGCTCTC XbaIAGGAGGAC SmaI GAATTCC NUE25 TCCCGGGTCATC 2657 3_NR_ ACTCGCTCTCGA SmaIATTCC NUE583 XbaI. NUE58 TATCTAGACAC 2658 35S_1F GGAGAGGACA 2918 pGXN3_EF_ GAATCAACCCA GGCTTCTTGAG XbaI CCAGAG SacI NUE58 TGAGCTCTCAAT 2659NUE58 TGAGCTCTCAT 2919 3_ER_ GCCGATCATCA 3_NR_ CAGAACCGGA SacI GTGCTAAGSacI AGAAGTTGG NUE58 TATCTAGAAAC 2660 3_NF_ AATGCCTTGGGT XbaI TTATCATCCNUE58 TGAGCTCTCATC 2661 3_NR_ AGAACCGGAAG SacI AAGTTGG NUE235 XbaI,NUE23 TATCTAGAATTG 2662 35S_1F GGAGAGGACA 2920 pGXN 5_EF_ AGCAGAGGAGCGGCTTCTTGAG XbaI CATG SacI NUE23 TGAGCTCCTACA 2663 NUE23 TGAGCTCTTAA2921 5_ER_ CAGGGTGCCAG 5_NR_ GTGCAAGTTGT SacI ATCTC SacI CAATCCTATTGNUE23 TATCTAGAGGA 2664 5_NF_ GCCATGGCCAA XbaI AATC NUE23 TGAGCTCTTAAG2665 5_NR_ TGCAAGTTGTCA SacI ATCCTATTG NUE231 35S_1F GGAGAGGACA 2922GGCTTCTTGAG GA NUE23 CCTGAGAGGG 2923 1_GA_ CGATCATATC R NUE513 XbaI,NUE51 AATCTAGAGAT 2666 35S_1F GGAGAGGACA 2924 pKSJ 3_NF_ GATGGTTTGATGGGCTTCTTGAG XbaI CAGATG SmaI NUE51 TCCCGGGCTAA 2667 NUE51 CTGCTTTGACA2925 3_NR_ CGTAGTTTCTTA 3_seqR TGGCTTAGAC SmaI CCAACCAAAC NUE516 SalI,NUE51 AATGTCGACGA 2668 p35S_F GGACAGGCTT 2926 pGXN 6_NF_ GAGAAGGGTGT 2CTTGAGATCCT SalI AATGAGCTG XbaI NUE51 TATCTAGATCAT 2669 NUE51TATCTAGATCA 2927 6_NR_ CAGTAGGGGTT 6_NR_ TCAGTAGGGG XbaI CCTATGTGG XbaITTCCTATGTGG NUE223 SalI, NUE22 AAAGTCGACCA 2670 35S_1F GGAGAGGACA 2928pGXN 3_NF_ AGAGGTAGCAC GGCTTCTTGAG SalI ATCCTCTCC XbaI NUE22 ATTCTAGACCG2671 NUE22 ATTCTAGACCG 2929 3_NR_ GATTGAACTAA 3_NR_ GATTGAACTA XbaITTAACGAC XbaI ATTAACGAC NUE540 SalI, NUE54 AAAGTCGACAG 2672 35S_1FGGAGAGGACA 2930 pGXN 0_NF_ GAAGATTGTGA GGCTTCTTGAG SalI GCATTGAAG XbaINUE54 ATTCTAGACACC 2673 NUE54 CATACCAACAT 2931 0_NR_ TAATGATCTCAC 0_ER_GTTCGACCAC XbaI TTGTAAGG NdeI NUE544 SalI, NUE54 TTAGTCGACAG 2674 35S_1FGGAGAGGACA 2932 pKSJ 4_EF_ CCTTGCCTTGTT GGCTTCTTGAG SalI TCTTC SmaINUE54 TCCCGGGCAAC 2675 NUE54 TCCCGGGCTTT 2933 4_ER_ TTATACACTCAA 4_NR_CATCCATGTGT SmaI CCAAAGC SmaI GCAGTG NUE54 TTAGTCGACCAT 2676 4_NF_ACACACACAGT SalI GAGAGGTAGG NUE54 TCCCGGGCTTTC 2677 4_NR_ ATCCATGTGTGCSmaI AGTG NUE560 XbaI, NUE56 AATCTAGAAGA 2678 35S_1F GGAGAGGACA 2934pGXN 0_EF_ AACCCAGAGGA GGCTTCTTGAG XbaI GCAGC SacI NUE56 CGAGCTCAAGG2679 NUE56 TGAGCTCCTAC 2935 0_ER_ GATTATTATTGC 0_NR_ TTCTAGGCCTT SacIAGGTTG SacI GTTGCTGC NUE56 AATCTAGAGAA 2680 0_NF_ GCAGGAAGGAA XbaIGCAGAG NUE56 TGAGCTCCTACT 2681 0_NR_ TCTAGGCCTTGT SacI TGCTGC NUE563XbaI, NUE56 AATCTAGAGAT 2682 NUE56 ATTCTAGATCA 2936 pGXN 3_EF_AACATCAGTAG 3_NF_ CAGCAACACA XbaI TTCGCAGC XbaI ATCACCAC SacI NUE56CGAGCTCAACA 2683 101_R AAGTTGGGTA 2937 3_ER_ CACTCACACCA ACGCCAGGGT SacIAAAGTCC NUE56 ATTCTAGATCAC 2684 3_NF_ AGCAACACAAT XbaI CACCAC NUE56TGAGCTCCACTG 2685 3_NR_ CTACTGAAGGC SacI AAATTC NUE565 XbaI NUE56ATTCTAGATTTT 2686 35S_1F GGAGAGGACA 2938 pGXN 5_EF_ CCTGGATTTTGTGGCTTCTTGAG XbaI TTTCTC SacI NUE56 TGAGCTCTCAAT 2687 NUE56 TGAGCTCCTAC2939 5_ER_ TAAAGAGTTAC 5_NR_ TTGAGCCTTCT SacI CCTAACG SacI AGCTCTGTTCNUE56 ATTCTAGAGATT 2688 5_NF_ TGGGGAAAAGC XbaI TATGG NUE56 TGAGCTCCTACT2689 5_NR_ TGAGCCTTCTAG SacI CTCTGTTC NUE566 SalI NUE56 TACGTCGACTTC2690 35S_1F GGAGAGGACA 2940 Topo 6_EF_ ACATGTCTTGAC GGCTTCTTGAG SalITAGTTCATATG NUE56 TAAGTCGACAC 2691 NUE56 CGAAGGCATA 2941 6_ER_GATACATTCAAT 6_R GACGTCTGTC SalI ACAATCACC NUE56 TTAGTCGACCTT 2692 6_NF_CCATCATGCTCC SalI CAAAG NUE56 TAAGTCGACTC 2693 6_NR_ AACTCAGCATC SalIACGTCTCAGC NUE586 SalI, NUE58 AATGTCGACTC 2694 35S_1F GGAGAGGACA 2942pKSJ 6_EF_ GTTTCTCCTCTA GGCTTCTTGAG SalI ACGTCAAC SmaI NUE58 TCCCGGGTCAG2695 NUE58 CATCGAAGCA 2943 6_ER_ CAGCTCTCTGTC 6_R CTTCTCAACTG SmaITGTTAC NUE58 ATAGTCGACGTT 2696 6_NF_ TAACATAGTTG SalI GGGCTAGG NUE58CCCCGGGATAA 2697 6_NR_ GCCAGGAGATG SmaI AAAGGAG NUE588 SalI, NUE58AAAGTCGACGA 2698 35S_1F GGAGAGGACA 2944 pGXN 8_NF_ TCGAAAAGAGAGGCTTCTTGAG SalI AGAGGAGC XbaI NUE58 ATTCTAGACTAA 2699 NUE58 ATTCTAGACTA2945 8_NR_ TCTCTCTCCCTC 8_NR_ ATCTCTCTCCC XbaI CCTCC XbaI TCCCTCC NUE59135S_1F GGAGAGGACA 2946 GGCTTCTTGAG GA NUE59 CTCTTGCAGCT 2947 1_GA_CTTGATCTTC R NUE206 XbaI, NUE20 ATTCTAGAATTT 2700 35S_1F GGAGAGGACA 2948pGN 6_EF_ ACACAGACTTG GGCTTCTTGAG XbaI TCGCTCTC SalI NUE20 TATCTAGACTTC2701 NUE20 TATCTAGATCA 2949 6_ER_ TGATTCAGTGAC 6_NR_ TCAGTGACTGT XbaITGTGAGC XbaI GAGCCTCGT NUE20 ATAGTCGACAA 2702 6_NF_ CAATGGACAAA SalITTTTGGAC NUE20 TATCTAGATCAT 2703 6_NR_ CAGTGACTGTG XbaI AGCCTCGT NUE208XbaI, NUE20 AATCTAGACTG 2704 35S_1F GGAGAGGACA 2950 pGN 8_EF_AAAGAGAGAGA GGCTTCTTGAG XbaI GGTATGGC SacI NUE20 TGAGCTCTGAAT 2705 NUE20TGAGCTCTTAT 2951 8_ER_ TAGTCATCTATT 8_NR_ TAGTCATCTAT SacI GGGTCC SacITGGGTCCTGAG NUE20 TATCTAGAAAC 2706 8_NF_ AATGGCAGGTG XbaI AGGCAACTCNUE20 TGAGCTCTTATT 2707 8_NR_ AGTCATCTATTG SacI GGTCCTGAG NUE209 SalI,NUE20 AATGTCGACTTT 2708 35S_1F GGAGAGGACA 2952 pGN 9_EF_ GTGATGACCCTTGGCTTCTTGAG SalI TTAAGG XbaI NUE20 ATTCTAGAGGT 2709 NUE20 ATTCTAGATTA2953 9_ER_ AGTTAGCCGGT 9_NR_ TTAGCCGGTCA XbaI CATGTTG XbaI TGTTGTAGTCNUE20 AATGTCGACAA 2710 9_NF_ CAATGGATTGG SalI GAAAAACAGC NUE20ATTCTAGATTAT 2711 9_NR_ TAGCCGGTCAT XbaI GTTGTAGTC NUE210 SalI NUE21TGAGTCGACGT 2712 35S_1F GGAGAGGACA 2954 pGN 0_EF_ CTTGAAATGTTTGGCTTCTTGAG SalI GGTGGGT XbaI NUE21 TATCTAGACTTA 2713 NUE21 TGTCTAGACTA2955 0_ER_ CTTGCCCTTTGC 0_NR_ TGCTATGAGG XbaI TTATGA XbaI AAAAGAAACTAAGC NUE21 AATGTCGACAA 2714 0_NF_ CAATGTTTGGTG SalI GGTTCAATGTG NUE21TGTCTAGACTAT 2715 0_NR_ GCTATGAGGAA XbaI AAGAAACTAAG C NUE211 35S_1FGGAGAGGACA 2956 GGCTTCTTGAG GeneArt NOS_R GCGGGACTCT 2957 AATCATAAAA ACCNUE212 XbaI NUE21 ATTCTAGAATAT 2716 35S_1F GGAGAGGACA 2958 pGN 2_EF_CATAATGAAAG GGCTTCTTGAG XbaI GGATTCG SacI NUE21 TGAGCTCCCATT 2717 NUE21TGAGCTCTTAT 2959 2_ER_ AGAACCGAGAC 2_NEW TAGAACCGAG SacI TGAAG _NR_ACTGAAGATA SacI CTTA NUE21 TATCTAGAAAC 2718 2_NF_ AATGAAAGGGA XbaITTCGCTCC NUE21 TGAGCTCTTATT 2719 2_NR_ AGAACCGAGAC SacI TGAAGATACTT ANUE221 EcoR NUE22 AAGATATCAAT 2720 35S_1F GGAGAGGACA 2960 pKSJ V 1_EF_GACTTTCCCCAT GGCTTCTTGAG EcoRV CTATCC NUE22 ACGATATCAAT 2721 NUE22ATGATATCCAT 2961 1_ER_ CGACCAACAAC 1_NR_ TACATGTGTGT EcoRV TAACATTACEcoRV ATCCGACG NUE22 AAGATATCCTTC 2722 1_NF_ TAATAATCAAC EcoRV CGACAGGNUE22 ATGATATCCATT 2723 1_NR_ ACATGTGTGTAT EcoRV CCGACG NUE222 SalI,NUE22 ATAGTCGACGG 2724 NUE22 AGTTGCATCGA 2962 pGN 2_EF_ GAAGTATCATT2_seq_F TCTTGATCTTG SalI AGTTCATTACC 1 XbaI NUE22 TATCTAGACTAG 2725101_ER CTGCAAGGCG 2963 2_ER_ TATCCCTAACGT ATTAAGTTGG XbaI AACAAAGACTCNUE22 AATGTCGACTTA 2726 2_NF_ CCATGGGAGAC SalI TATAACATG NUE22TATCTAGACTAC 2727 2_NR_ TAACGTAACAA XbaI AGACTCTTCACA NUE229 XbaI NUE22TATCTAGACTGT 2728 NUE22 GCAAGGTTAG 2964 pGN 9_EF_ CTGTTTGCCTGT 9_seq_FCTTCATGACG XbaI CGAG 1 SmaI NUE22 TCCCGGGATACT 2729 101_ER GAAACACCAT2965 9_ER_ CAAATCAAATG CTTCGTTCTTG SmaI AAAGTCCG NUE22 CATCTAGACAA 27309_NF_ CAATGGCGAGG XbaI ATGATC NUE22 TCCCGGGTTAG 2731 9_NR_ ATAGAAGTTTATSmaI CCCATCAGGG NUE254 SalI NUE25 AATGTCGACAG 2732 NUE25 AATGTCGACCT2966 pGN 4_EF_ TCTGCACTGGA 4_NF_ GGAAGGACAG SalI AGGACAG SalI CATGTCGXbaI NUE25 TATCTAGACTTG 2733 101_R AAGTTGGGTA 2967 4_ER_ TTGCCAGCATCTACGCCAGGGT XbaI CTTATG NUE25 AATGTCGACCT 2734 4_NF_ GGAAGGACAGC SalIATGTCG NUE25 TATCTAGACTAT 2735 4_NR_ GACTAGCTGAT XbaI GGAGTCCTCC NUE267NUE26 CTTCTTCAATGG 2736 NUE26 CTTCTTCAATG 2968 Topo 7_F CGACGG 7_FGCGACGG NUE26 TAGTCATGCAA 2737 101_ER GAAACACCAT 2969 7_R ATATTTAATCTTCTTCGTTCTTG GGAACCC NUE519 SalI, NUE51 TTAGTCGACTTA 2738 NUE51TTAGTCGACTT 2970 pGN 9_NF_ AGATGGCCAAG 9_NF_ AAGATGGCCA SalI GTTAACGSalI AGGTTAACG XbaI NUE51 TATCTAGACTAA 2739 101_ER CTGCAAGGCG 2971 9_NR_TGCCGTTGCTTC ATTAAGTTGG XbaI TAGTAATAG NUE549 XbaI, NUE54 TATCTAGATCCT2740 NUE54 CAGCTGTGGA 2972 pGN 9_EF_ CTCCCTAGCTAG 9_seq_F AGGCATCAACXbaI CAAG 3 SacI NUE54 TGAGCTCCTAAT 2741 101_R AAGTTGGGTA 2973 9_ER_CACCCTGGCTGT ACGCCAGGGT SacI TGAC NUE54 TATCTAGATCCC 2742 9_NF_TAGCTAGCAAG XbaI CTCTAG NUE54 TGAGCTCCCTTA 2743 9_NR_ ATGCCATGCTGC SacIG NUE572 XbaI, NUE57 ATTCTAGATACA 2744 35S_1F GGAGAGGACA 2974 pGN 2_NF_TCGTCTTCACCT GGCTTCTTGAG XbaI AATTTTC SacI NUE57 CGAGCTCAACA 2745 NUE57CGAGCTCAAC 2975 2_NR_ AGCAAACTAAA 2_NR_ AAGCAAACTA SacI CGTGAAC SacIAACGTGAAC NUE592 EcoR NUE59 ATGATATCAAA 2746 35S_1F GGAGAGGACA 2976 pKSJV 2_EF_ TCCGGTGGAC GGCTTCTTGAG EcoRV NUE59 TAGATATCCAA 2747 NUE59TAGATATCGTT 2977 2_ER_ CACTCACTAGG 2_NR_ GAACGCTCCA EcoRV GAGCACAG EcoRVCATCATG NUE59 TAGATATCAGA 2748 2_NF_ ATTCGCAGGGA EcoRV TGCC NUE59TAGATATCGTTG 2749 2_NR_ AACGCTCCACA EcoRV TCATG NUE248 XbaI, NUE24GCTCTAGAAGG 2750 NUE24 GCTCTAGAAG 2978 pGN 8_NF_ CGAGATGTGGG 8_NF_GCGAGATGTG XbaI AGTC XbaI GGAGTC SacI NUE24 TGAGCTCCTACT 2751 NOS_RGCGGGACTCT 2979 8_NR_ AGGCCTTCTCCT AATCATAAAA SacI TTGTTG ACC NUE590SacI NUE59 AATCTAGACAA 2752 35S_1F GGAGAGGACA 2980 TopoB 0_EF_CTGCAACTGCA GGCTTCTTGAG XbaI ACTAGC NUE59 CGAGCTCACAG 2753 0_ER_CTAAACATCAA SacI TCCTCTTC NUE59 TGAGCTCTGCA 2754 NUE59 TGAGCTCCTCA 29810_NF_ AGCAATCACCA 0_NR_ TTTTATTTGCT SacI GTTTG SacI GCGTG NUE59TGAGCTCCTCAT 2755 0_NR_ TTTATTTGCTGC SacI GTG NUE245 35S_1F GGAGAGGACA2982 GGCTTCTTGAG GA NUE24 CTCGGTGTTCT 2983 5_GA_ TGATGGTCAC R1 NUE52035S_1F GGAGAGGACA 2984 GGCTTCTTGAG GA NUE52 TTCTTGACCTT 2985 0_GA_GGTCAGCTTG R2 NUE574 SmaI NUE57 Agattagtcccaaagatta 2756 35S_1FGGAGAGGACA 2986 Topo 4_EF_ ttcg GGCTTCTTGAG maI NUE57 Gacattgtggggaagcta2757 NUE57 gcatgtaattgtagctttc 2987 4_ER_ ct 4_NR_ tttt maI maI NUE57Gatacaaagaattcgcttt 2758 4_NF_S gc maI NUE57 gcatgtaattgtagctttctt 27594_NR_ tt maI NUE224 XbaI. NUE22 TATCTAGAGTTT 2760 p35S_F GGAGAGGACA 2988pGXN 4_EF_ GCTTGCTTACCA 1 GGCTTCTTGAG XbaI GGAG SmaI NUE22 TCCCGGGTTAGC2761 NUE22 TCCCGGGTTAG 2989 4_ER_ AGCATCGATCG 4_ER_ CAGCATCGATC SmaITACACTAG SmaI GTACACTAG NUE225 SalI, NUE22 AATGTCGACGA 2762 p35S_FGGAGAGGACA 2990 pGXN 5_NF_ GTTTACAAGAG 1 GGCTTCTTGAG SalI ACCCAGACG XbaINUE22 ACTCTAGAATTC 2763 NUE22 ACTCTAGAATT 2991 5_NR_ AGTCATAGATC 5_NR_CAGTCATAGAT XbaI GCCTTG XbaI CGCCTTG NUE230 p35S_F GGAGAGGACA 2992 1GGCTTCTTGAG GA NUE23 GGATCTTGATG 2993 0_GA_ TACACGTTTGG R1 NUE234 p35S_FGGAGAGGACA 2994 1 GGCTTCTTGAG GA NUE23 CGATGTTGCAC 2995 4_GA_ CTCTTTGGR1 NUE239 p35S_F GGAGAGGACA 2996 1 GGCTTCTTGAG GA NUE23 CGAAATCCTCT 29979_GA_ GGGAATGAC R1 NUE240 p35S_F GGAGAGGACA 2998 1 GGCTTCTTGAG GA NUE24CCTCAGTAGA 2999 0_GA_ GAGAGACTCG R1 TCG NUE246 p35S_F GGAGAGGACA 3000 1GGCTTCTTGAG GA NUE24 CAACACTTGCA 3001 6_GA_ TCACCCTAGTC R1 NUE249 p35S_FGGAGAGGACA 3002 1 GGCTTCTTGAG GA NUE24 CCACCTCAAG 3003 9_GA_ AACAGTAACGR1 AG NUE250 p35S_F GGAGAGGACA 3004 1 GGCTTCTTGAG GA NUE25 GAAGGTAGAG3005 0_GA_ TGCAGCATGG R1 NUE252 XbaI, NUE25 TATCTAGATTGG 2764 p35S_FGGAGAGGACA 3006 pGXN 2_EF_ TCACAGGGGAT 1 GGCTTCTTGAG XbaI AGGC SacINUE25 TGAGCTCCTAA 2765 NUE25 SacI 3007 2_ER_ GATGCTGCTTTC 2_NR_TGAGCTCCTAC SacI TCAGACTATG TATGCCAAAG AACCTTCATG NUE25 TATCTAGAGAA 27662_NF_ ATTGTGTTTGTT XbaI TGATGGG NUE25 TGAGCTCCTACT 2767 2_NR_ATGCCAAAGAA SacI CCTTCATG NUE265 XbaI, NUE26 TATCTAGAGAG 2768 p35S_FGGAGAGGACA 3008 pGXN 5_NF_ AAATGACAAGT 1 GGCTTCTTGAG XbaI GTCTGGAAG SacINUE26 TGAGCTCGGAG 2769 NUE26 TGAGCTCGGA 3009 5_NR_ TGATCACTACTG 5_NR_GTGATCACTAC SacI CTTCTCC SacI TGCTTCTCC NUE268 SalI NUE26 AATGTCGACTG2770 p35S_F GGAGAGGACA 3010 pGXN 8_NF_ AAGATGGCTGA 1 GGCTTCTTGAG SalICGATTTG XbaI NUE26 TATCTAGACTAG 2771  NUE26 TATCTAGACTA 3011 8_NR_TCTTAGCCACCA 8_NR_ GTCTTAGCCAC XbaI CCAGAAC XbaI CACCAGAAC NUE514 XbaI,NUE51 AATCTAGAGGA 2772 p35S_F GGAGAGGACA 3012 pGXN 4_EF_ TTGAGACATGC 1GGCTTCTTGAG XbaI ACTTAACAG SacI NUE51 TGAGCTCTTTTG 2773 NUE51TGAGCTCCTAC 3013 4_ER_ AGCACCTCTTAT 4_NR_ AATACACCTCT SacI TTAGC SacITGACATCCTTC NUE51 AATCTAGAACT 2774 4_NF_ CATCAGCAACT XbaI ACAACGTG NUE51TGAGCTCCTACA 2775 4_NR_ ATACACCTCTTG SacI ACATCCTTC NUE515 SalI, NUE51TAAGTCGACGA 2776 p35S_F GGAGAGGACA 3014 pGXN 5_NF_ TACAATGAGAA 1GGCTTCTTGAG SalI, TGTTAGTTCTTC G XbaI NUE51 TATCTAGATCA 2777 NUE51TATCTAGATCAT 3015 5_NR_ CACCATCGTCTT 5_NR_ TCACCATCGTC XbaI ATCAATGAAGXbaI TTATCAATGAA G NUE523 SmaI, NUE52 ACCCGGGTCGT 2778 p35S_F GGAGAGGACA3016 Topo 3_EF_ CTCATCAATTCA 1 GGCTTCTTGAG SmaI AGATCC SacI NUE52TGAGCTCCCCTT 2779 NUE52 TGAGCTCCCCT 3017 3_ER_ CAAACTAATCA 3_ER_TCAAACTAATC SacI ATCTTG SacI AATCTTG NUE525 p35S_F GGAGAGGACA 3018 pQXY1 GGCTTCTTGAG N GA NUE52 GTACTGAAGCT 3019 5_GA_ CGTCCTGGAC R NUE527 XbaINUE52 AATCTAGAAAG 2780 p35S_F GGAGAGGACA 3020 pKSJ 7 EF AGCACCACCAG 1GGCTTCTTGAG XbaI AGCAG EcoR NUE52 TTGATATCCTTT 2781 NUE52 TTGATATCCTT3021 V 7_ER_ ATGTCACCATTC 7_ER_ TATGTCACCAT EcoRV ATCTCAG coRV TCATCTCAGNUE532 XbaI, NUE53 AATCTAGACTG 2782 p35S_F GGAGAGGACA 3022 pGXN 2_EF_GTTTAGGAGAC 1 GGCTTCTTGAG XbaI GAAAAGG SacI NUE53 AGAGCTCCTAC 2783 NUE53AGAGCTCCTATC 3023 2_ER_ TCAACTCCATCG 2_NR_ TACTCAACTTC SacI CCTCAG SacITCTGATGATTC TC NUE53 AATCTAGAAGT 2784 2_NF_ GCTCTCCGGTTT XbaI GAGG_NUE53 AGAGCTCCTACT 2785 2_NR_ ACTCAACTTCTC SacI TGATGATTCTC NUE533p35S_F GGAGAGGACA 3024 pQXY 1 GGCTTCTTGAG N GA NUE53 GGTTAGACAC 30253_GA_ GAGCTTCTCAG R AC NUE536 XbaI, NUE53 ATTCTAGAGCCT 2786 p35S_FGGAGAGGACA 3026 pGXN 6_EF_ TCTGATTCCCAC 1 GGCTTCTTGAG XbaI TCC SacINUE53 TGAGCTCTGGA 2787 NUE53 CGAGCTCAAA 3027 6_ER_ GTATCTGGTTTA 6_NR_GTCTCACTCCG SacI GTTCGTC SacI CACTACAC NUE53 AATCTAGACCT 2788 6_NF_ACTATACTTGCA XbaI ACCTCTCC NUE53 CGAGCTCAAAG 2789 6_NR_ TCTCACTCCGCASacI CTACAC NUE547 p35S_F GGAGAGGACA 3028 pQXY 1 GGCTTCTTGAG N GA NUE54GTGTGCAGCTC 3029 7_GA_ GAACTTGG R NUE550 SmaI NUE55 ACCCGGGGTAA 2790p35S_F GGACAGGCTT 3030 pKSJ 0_EF_ CACTATCAAGA 2 CTTGAGATCCT SmaIGACGATGAAG NUE55 TCCCGGGGTTTA 2791 NUE55 TCCCGGGAATC 3031 0_ER_CATTGTTCTCGT 0_NR_ TTTATTAACGA SmaI TTCAAATC SmaI AACAGCAG NUE55ACCCGGGCTAT 2792 0_NF_ CAAGAGACGAT SmaI GAAGGTTG NUE55 TCCCGGGAATCT 27930_NR_ TTATTAACGAA SmaI ACAGCAG NUE564 XbaI, NUE56 AATCTAGACTTC 2794p35S_F GGAGAGGACA 3032 pGXN 4_EF_ AAGCAGGCAGC 1 GGCTTCTTGAG XbaI ACACSacI NUE56 CGAGCTCAAAG 2795 NUE56 TGAGCTCCTAC 3033 4_ER_ GGTCCATCATA4_NR_ ATGTCCCTTAG SacI ATCACAG SacI ATTGCTCTATT C NUE56 TATCTAGAGGA 27964_NF_ AACCTTGAGCC XbaI ATGG NUE56 TGAGCTCCTACA 2797 4_NR_ TGTCCCTTAGATSacI TGCTCTATTC NUE576 SalI, NUE57 AAAGTCGACAG 2798 p35S_F GGAGAGGACA3034 pGXN 6_EF_ GAACAGCAACA 1 GGCTTCTTGAG SalI AAAGTAAGC SmaI NUE57TCCCGGGCTAA 2799 NUE57 TCCCGGGCTAA 3035 6_ER_ ACTGTCCCATTC 6_NR_GTAGCATGAG SmaI TGCGTG SmaI TCTAGAGCTTG G NUE57 AAAGTCGACCA 2800 6_NF_ACAACCACACA SalI CACTCACAG NUE57 TCCCGGGCTAA 2801 6_NR_ GTAGCATGAGT SmaICTAGAGCTTGG NUE579 SalI, NUE57 AATGTCGACTCT 2802 p35S_F GGAGAGGACA 3036pGXN 9_NF_ CAAAACCCTAA 1 GGCTTCTTGAG SalI CTGTTTCC_ XbaI NUE57ATTCTAGACAG 2803 NUE57 ATTCTAGACAG 3037 9_NR_ GATAATAGATA 9_NR_GATAATAGAT XbaI GTCACACGAGG XbaI AGTCACACGA GG NUE581 SalI, NUE58AAAGTCGACCA 2804 p35S_F GGAGAGGACA 3038 pGXN 1_EF_ AAAGAATCTGT 1GGCTTCTTGAG SalI CTTCTTCTCTG XbaI NUE58 ATTCTAGACTAT 2805 NUE58ACTCTAGATTA 3039 1_ER_ CCAAGAAGGAA 1_NR_ GAACCACAAA XbaI CAATGAGG XbaIAGATTACAAC ATC NUE58 AAAGTCGACGG 2806 1_NF_ TAAAATATCTTT SalI CTTGTGCAGNUE58 ACTCTAGATTAG 2807 1_NR_ AACCACAAAAG XbaI ATTACAACATC MAB52 6669FTCAGCCACCCA 3040 pGN AACCATGAC GA MAB52 GAAGTCCTGA 3041 _R_SeqGACCGTTGATA G MAB10 MAB10 GTTCCAGTTGAG 2808 T7_1 TACGACTCACT 3042 pGN 66_EF CGAGCAG ATAGGGCGA EcoR MAB10 TTGATATCCCAG 2809 MAB10 AAGATATCGT3043 V, 6_ER TCTGTTTATTGC 6_NR_ GCTAAACTATA _EcoRV ATCATC EcoRVCATCAAACGT G PstI MAB10 AACTGCAGGAT 2810 6 CATCCTCACATT _NF_ GCGAG PstIMAB10 AAGATATCGTG 2811 6_NR_ CTAAACTATAC EcoRV ATCAAACGTG NUE251 35S_1FGGAGAGGACA 3044 GGCTTCTTGAG GA NUE25 GAAGTACCAC 3045 1_GA_ CAGTTGAAGA RAGC NUE545 SalI, NUE54 TATGTCGACAG 2812 NUE54 GCAACAATTGT 3046 pGXN5_NF_ GTTATGGGGAA 5_F GGAGTCAACA SalI GAAGCTAG C XbaI NUE54 TATCTAGATCAT2813 101_R AAGTTGGGTA 3047 5_NR_ CAGTAGCCACG ACGCCAGGGT XbaI AACTTGTCTAGNUE570 Sal, NUE57 TTCGTCGACTAA 2814 NUE57 CTTTGAGACGT 3048 pKSJ 0_NFGCACAAATGGC 0 SeqF  TAGCTGTTGAG Sal GACTC Sma NUE57 ACCCGGGTCAA 2815101_R AAGTTGGGTA 3049 0 NR GGAGCTGAAAC ACGCCAGGGT Sma ACTAGAGTTACTNUE571 Sal, NUE57 GTAGTCGACTTC 2816 NUE57 GTAGTCGACTT 3050 pGXN 1_NF_ACATGGGAAAG 1_NF_ CACATGGGAA Sal GATAAGAC Sal AGGATAAGAC Xba NUE57AATCTAGATCA 2817 101_R AAGTTGGGTA 3051 1 NR CTGATATAGTCC ACGCCAGGGT XbaACGTCCTAAGG NUE578 XbaI, NUE57 AATCTAGAATA 2818 35S_1F GGAGAGGACA 3052pGXN 8_EF_ TCCTCCCATTCT GGCTTCTTGAG XbaI CATTCTG SmaI NUE57 TCCCGGGCTAAT2819 NUE57 TCCCGGGCTAA 3053 8_ER_ GCAATCTCCAA 8_NR_ GAAAAGGTAG SmaICTCCAAG SmaI GAGAAGGAAG G NUE57 AATCTAGAAGC 2820 8_NF_ GGAGAAGAGGA XbaIAGGAG NUE57 TCCCGGGCTAA 2821 8_NR_ GAAAAGGTAGG SmaI AGAAGGAAGG NUE580XbaI, NUE58 AATCTAGACGG 2822 35S_1F GGAGAGGACA 3054 pGXN 0_NF_AATATACATTTG GGCTTCTTGAG XbaI CTTTGTG SmaI NUE58 TCCCGGGCTACT 2823 NUE58TCCCGGGCTAC 3055 0_NR_ GCTGAATGCTCT 0_NR_ TGCTGAATGCT SmaI CTTTGC SmaICTCTTTGC NUE582 XbaI NUE58 AATCTAGAAAT 2824 35S_1F GGAGAGGACA 3056 pGXN2_NF_ CATCCTTCCCCA GGCTTCTTGAG XbaI ACCTC SmaI NUE58 CCCCGGGACCC 2825NUE58 CCCCGGGACC 3057 2_NR_ AAACAGTCATG 2_NR_ CAAACAGTCA SmaI CTAGG SmaITGCTAGG NUE584 SalI, NUE58 AAAGTCGACAA 2826 35S_1F GGAGAGGACA 3058 pGXN4_NF_ GGTTGGAGATT GGCTTCTTGAG SalI GTGAAATTG SacI NUE58 CGAGCTCATACT2827 NUE58 CGAGCTCATAC 3059 4_NR_ CTACGTTCCCGT 4_NR_ TCTACGTTCCC SacIGTGG SacI GTGTGG NUE593 35S_1F GGAGAGGACA 3060 GGCTTCTTGAG GA NUE59GTAGCCTGAA 3061 3_GA_ CAGCAGAACC R CT1 SmaI Reverse AAGTTGGGTAA 2828 pKSCGCCAGGGT SacI Forward GGTGGCTCCTAC 2829 AAATGCCATC CT11 SmaI ReverseAAGTTGGGTAA 2830 pKS CGCCAGGGT SacI Forward GGTGGCTCCTAC 2831 AAATGCCATCCT2 XbaI Reverse AAGTTGGGTAA 2832 pKS CGCCAGGGT Forward ATGGGGCAACA 2833TCACTTGGG CT20 SmaI Reverse AAGTTGGGTAA 2834 pKS CGCCAGGGT SacI ForwardGGTGGCTCCTAC 2835 AAATGCCATC CT22 SmaI Reverse AAGTTGGGTAA 2836 pKSCGCCAGGGT SacI Forward GGTGGCTCCTAC 2837 AAATGCCATC CT27 SmaI ReverseAAGTTGGGTAA 2838 pKS CGCCAGGGT EcoR Forward GGTGGCTCCTAC 2839 VAAATGCCATC CT3 SmaI Reverse AAGTTGGGTAA 2840 pKS CGCCAGGGT SacI ForwardGGTGGCTCCTAC 2841 AAATGCCATC CT40 SmaI Reverse AAGTTGGGTAA 2842 pKSCGCCAGGGT SacI Forward GGTGGCTCCTAC 2843 AAATGCCATC CT6 SmaI ReverseAAGTTGGGTAA 2844 pKS CGCCAGGGT SacI Forward GGTGGCTCCTAC 2845 AAATGCCATCCT7 SmaI Reverse AAGTTGGGTAA 2846 pKS CGCCAGGGT EcoR ForwardGGTGGCTCCTAC 2847 V AAATGCCATC CT71 XbaI Reverse AAGTTGGGTAA 2848 pKSCGCCAGGGT SacI Forward GGTGGCTCCTAC 2849 AAATGCCATC CT74 SmaI ReverseAAGTTGGGTAA 2850 pKS CGCCAGGGT SacI Forward GGTGGCTCCTAC 2851 AAATGCCATCCT75 SmaI Reverse AAGTTGGGTAA 2852 pKS CGCCAGGGT EcoR ForwardGGTGGCTCCTAC 2853 V AAATGCCATC CT76 SmaI Reverse AAGTTGGGTAA 2854 pKSCGCCAGGGT SacI Forward GGTGGCTCCTAC 2855 AAATGCCATC CT81 SmaI ReverseAAGTTGGGTAA 2856 pKS CGCCAGGGT SacI Forward GGTGGCTCCTAC 2857 AAATGCCATCCT82 SmaI Reverse AAGTTGGGTAA 2858 pKS CGCCAGGGT SacI ForwardGGTGGCTCCTAC 2859 AAATGCCATC

Table 22: Provided are the sequences of the primers used for cloning theindicated genes and for screening of cloned binary plasmids. Primers areprovided from 5′→3′. “EF”=external forward primer; “ER” external reverseprimer; “NF” nested forward primer; “NR” nested reverse primer. Unlessindicated otherwise, all genes were cloned from RNA molecules.“GA”=GeneArt, synthetically prepared genes; “Enz.”=Enzyme;“Plas.”=Plasmid.

Each digested PCR product was inserted into a high copy vectororiginated from pBlue-script KS plasmid vector (pBlue-script KS plasmidvector, Hypertext Transfer Protocol://World Wide Web (dot) stratagene(dot) com/manuals/212205 (dot) pdf). In case of the high copy vectororiginated from pBlue-script KS plasmid vector (pGN) PCR product wasinserted in the high copy plasmid upstream to the NOS terminator (SEQ IDNO:3064) originated from pBI 101.3 binary vector (GenBank Accession No.U12640, nucleotides 4417 to 4693). In other cases the PCR product wasinserted into the pCR®-BluntII-TOPO® high copy vector (ZeroBlunt® TOPO®PCR cloning Kit, Invitrogene). Some of the genes were syntheticallysynthesized ordered from a commercial supplier (GeneArt, GmbH) thosegenes were received into the pQXYN, pGXN high copy vectors obtained fromthe suppliers.

Sequencing of the inserted genes was performed, using the ABI 377sequencer (Applied Biosystems). In some cases, after confirming thesequences of the cloned genes, the cloned cDNA accompanied with the NOSterminator was introduced into the binary vectors pGI containing the 35Spromoter via digestion with appropriate restriction endonucleases. Inother cases the cloned cDNA accompanied with the 35S promoter wasintroduced into the pGI vector. In any case the insert was followed bysingle copy of the NOS terminator (SEQ ID NO: 3064). The digestedproducts and the linearized plasmid vector were ligated using T4 DNAligase enzyme (Roche, Switzerland)

For some of the cloned polynucleotides, instead of amplifying thesequence from cDNA, synthetic sequences were ordered from a commercialsupplier (GeneArt, GmbH). Thus, no primers were used for theamplification of the synthetic genes. To optimize the coding sequencesof the synthetic genes, codon-usage Tables calculated from planttranscriptomes were used (example of such Tables can be found in theCodon Usage Database available online at Hypertext TransferProtocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/). Theoptimized coding sequences is designed in a way that no changes areintroduced in the encoded amino acid sequence while using codonspreferred for expression in dicotyledonous plants mainly tomato andArabidopsis; and monocotyledonous plants such as maize. Such optimizedsequences promote better translation rate and therefore higher proteinexpression levels. To the optimized sequences flanking additional uniquerestriction enzymes sites were added—to facilitate the cloning of thegenes into the binary vectors.

The pPI and pGI binary vector plasmids were used to introduce the geneconstructs into plants. pPI plasmid was constructed by inserting asynthetic poly-(A) signal sequence, originating from pGL3 basic plasmidvector (Promega, Acc. No. U47295; bp 4658-4811) into the HindIIIrestriction site of the binary vector pBI101.3 (Clontech, Acc. No.U12640). In some cases the backbone binary plasmid used was pGI which issimilar to pPI but the GUS gene was replaced by the GUS-Intron gene(Vancanneyt. G, et al MGG 220, 245-50, 1990). pPI or pGI plasmid wasused to clone the polynucleotide sequences, initially under the controlof 35S promoter [Odell, J T, et al. Nature 313, 810-812 (28 Feb. 1985);SEQ ID NO: 3063] or Arabidopsis thaliana promoter At6669 (SEQ IDNO:3064, PCT Publication No. WO2004/104162). The At6669 or the CaMV 35Spromoter sequence (set forth in SEQ ID NO: 3063) is inserted in the pPIor pGI binary vector, upstream to the cloned genes by using therestriction enzymes HindIII and SalI or BamHI (Roche, Switzerland). Thedigested PCR product and the linearized plasmid vector were ligatedusing T4 DNA ligase enzyme (Roche, Switzerland), as described above.

60 μL of E. coli, strain DH5-α competent cells (about 10⁹ cells/mL) weretransformed using 1 μl of ligation reaction mixture by electroporation,using a MicroPulser electroporator (Biorad), 0.2 cm cuvettes (Biorad)and EC-2 electroporation program (Biorad). E. coli cells were grown on0.8 mL LB liquid medium at 37° C. for 1 hrs and 0.2 mL of the cellsuspension were plated on LB-agar plates supplemented with theantibiotics kanamycin 50 mg/L (Sigma). Plates were then incubated at 37°C. for 16 hrs. Bacteria colonies were grown and expression was confirmedby PCR amplification using the primers sets detailed in Table 22, above,which were designed to span the inserted sequence in the binary vector.

PCR products were separated on 1.5% agarose gels and product sizes wereestimated by comparing to DNA ladder (MBI Fermentas).

TABLE 23 Cloned sequences SEQ ID NO: SEQ ID NO: of of cloned or Gene theencoded Cloning synthetic gene Name polypeptide Cluster Method 2398 CT12523 cotton|gb164|AI725990_T1 cloned 2399 CT11 2524cotton|gb164|AI725968_T1 cloned 2400 CT2 2525 cotton|gb164|AI727334_T1cloned 2401 CT20 2526 cotton|gb164|AI726497_T1 cloned 2402 CT22 2527cotton|gb164|BG440027_T1 cloned 2403 CT27 2528 cotton|gb164|AF336280_T1cloned 2404 CT3 144 cotton|gb164|AI725456_T1 cloned 2405 CT40 145cotton|gb164|BE052317_T1 cloned 2406 CT6 2529 cotton|gb164|AI726479_T1cloned 2407 CT7 147 cotton|gb164|AI727027_T1 cloned 2408 CT71 148cotton|gb164|AI725508_T1 cloned 2409 CT74 149 cotton|gb164|AI725950_T1cloned 2410 CT75 2530 cotton|gb164|AI726599_T1 cloned 2411 CT76 2531cotton|gb164|AI726155_T1 cloned 2412 CT81 2532 cotton|gb164|AI726693_T1cloned 2413 CT82 153 cotton|gb164|BQ402794_T1 cloned 2414 MAB106 154barley|gb157.2|AL450627_T1 cloned 2415 MAB52 155rice|gb157.2|AU070543_T1 synthesized_ optimized 2416 NUE206 158arabidopsis|gb165|AT4G24960_T1 cloned 2417 NUE208 2533tomato|gb164|BG124666_T1 cloned 2418 NUE209 160 tomato|gb164|BG134403_T1cloned 2419 NUE210 2534 tomato|gb157|TOMTRALTAB_T1 cloned 2420 NUE211162 rice|gb157.2|AU174544_T1 synthesized_ optimized 2421 NUE212 163cotton|gb164|CO081293_T1 cloned 2422 NUE221 164 rice|gb157.2|BI305241_T1cloned 2423 NUE222 165 arabidopsis|gb1T615|AT1G31820_T1 cloned 2424NUE223 166 rice|gb157.2|AW069985_T1 cloned 2425 NUE224 167rice|gb157.2|AW155063_T1 cloned 2426 NUE225 168 rice|gb157.2|BE039221_T1cloned 2427 NUE227 169 rice|gb157.2|AU056888_T1 cloned 2428 NUE228 170rice|gb157.2|AA753730_T1 synthesized_ optimized 2429 NUE229 2535maize|gb164|AW455682_T1 cloned 2430 NUE230 172 rice|gb157.2|AA749861_T1synthesized_ optimized 2431 NUE231 173 rice|gb157.2|AK108994_T1synthesized_ optimized 2432 NUE233 174 rice|gb157.2|CB640732_T1 cloned2433 NUE234 175 poplar|gb157.2|BU868634_T1 synthesized_ optimized 2434NUE235 176 soybean|gb162|CA852963_T1 cloned 2435 NUE237 177rice|gb157.2|BI811377_T1 cloned 2436 NUE239 178poplar|gb157.2|BU880014_T1 synthesized_ optimized 2437 NUE240 179poplar|gb157.2|AJ407707_T1 synthesized_ optimized 2438 NUE241 180tomato|gb164|BG129806_T1 cloned 2439 NUE242 2536tomato|gb164|B0791300_T1 cloned 2440 NUE244 182soybean|gb162|CF808561_T1 cloned 2441 NUE245 2537rice|gb157.2|AT003383_T1 synthesized_ optimized 2442 NUE246 184grape|gb160|CF207859_T1 synthesized 2443 NUE248 2538maize|gb157|BG354535_T1 cloned 2444 NUE249 186 rice|gb157.2|AU029933_T1synthesized_ optimized 2445 NUE250 187 rice|gb157.2|AK102239_T1synthesized_ optimized 2446 NUE251 188 sorghum|gb161.xeno|AI947781_T1synthesized_ optimized 2447 NUE252 189 arabidopsis|gb165|AT1G58030_T1cloned 2448 NUE253 190 rice|gb157.2|AF145730_T1 cloned 2449 NUE254 2539maize|gb164|AI600563_T1 cloned 2450 NUE255 2540 rice|gb157.2|CB000630_T1cloned 2451 NUE256 193 wheat|gb164|BE415875_T1 synthesized_ optimized2452 NUE265 194 rice|gb157.2|BE039218_T1 cloned 2453 NUE267 195arabidopsis|gb165|AT5G60680_T1 cloned 2454 NUE268 196rice|gb157.2|AA750934_T1 cloned 2455 NUE269 2541cotton|gb164|AI730085_T1 cloned 2456 NUE49 2542 maize|gb154|AW037179_T1synthesized_ optimized 2457 NUE50 2543 maize|gb164|AW287760_T1 cloned2458 NUE511 2544 maize|gb157|AW360667_T1 cloned 2459 NUE512 201arabidopsis|gb157.2|AT5G23460_T1 cloned 2460 NUE513 2545arabidopsis|gb157.2|AT3G26100_T1 cloned 2461 NUE514 2546soybean|gb162|SOYHPR_T1 cloned 2462 NUE515 2547arabidopsis|gb5|AT1G44920_T1 cloned 2463 NUE516 205arabidopsis|gb157.2|AT1G48210_T1 cloned 2464 NUE519 2548wheat|gb164|BE445396_T1 cloned 2465 NUE520 207 rice|gb157.2|BI305493_T1synthesized 2466 NUE521 208 rice|gb157.2|AU077950_T1 cloned 2467 NUE523209 sorghum|gb161.xeno|AI901439_T1 cloned 2468 NUE525 210sorghum|gb161.xeno|AW052978_T1 synthesized_ optimized 2469 NUE527 211sorghum|gb161.xeno|AW055409_T1 cloned 2470 NUE528 212sorghum|gb161.xeno|AI372194_T1 cloned 2471 NUE531 213rice|gb157.2|BI805136_T1 synthesized_ optimized 2472 NUE532 214maize|gb164|AW054475_T1 cloned 2473 NUE533 215 soybean|gb166|AW350050_T1cloned 2474 NUE535 2549 sorghum|gb161.crp|BE599042_T1 cloned 2475 NUE536217 maize|gb164|BQ279657_T1 cloned 2476 NUE537 218barley|gb157.2|AJ234408_T1 cloned 2477 NUE538 219sorghum|gb161.xeno|AW923729_T1 cloned 2478 NUE539 220rice|gb157.2|AW155216_T1 synthesized_ optimized 2479 NUE540 2550arabidopsis|gb157.2|AT1G13980_T1 cloned 2480 NUE542 2551arabidopsis|gb157.2|AT3G46280_T1 cloned 2481 NUE543 223rice|gb157.2|AK063415_T1 synthesized_ optimized 2482 NUE544 2552cotton|gb164|BQ412384_T1 cloned 2483 NUE545 2553cotton|gb164|A1055737_T1 cloned 2484 NUE547 226sorghum|gb161.xeno|BI139559_T1 synthesized_ optimized 2485 NUE548 227sorghum|gb161.xeno|BQ279657_T1 cloned 2486 NUE549 228sorghum|gb161.xeno|AF019147_T1 cloned 2487 NUE550 229canola|gb161|EE559843_T1 cloned 2488 NUE551 2554barley|gb157.3|BE420701_T1 cloned 2489 NUE553 231barley|gb157.3|BE421829_T1 cloned 2490 NUE554 232sorghum|gb161.xeno|AA011880_T1 cloned 2491 NUE560 233rice|gb157.2|BE229552_T1 cloned 2492 NUE562 2555rice|gb157.2|BE039784_T1 cloned 2493 NUE563 235 rice|gb157.2|AU057884_T1cloned 2494 NUE564 236 maize|gb164|AI619269_T1 cloned 2495 NUE565 237arabidopsis|gb157.2|AT5G15080_T1 cloned 2496 NUE566 238arabidopsis|gb165|AT2G4370_T1 cloned 2497 NUE567 239arabidopsis|gb165|AT1G6068_T1 cloned 2498 NUE568 240arabidopsis|gb165|AT1G7845_T1 cloned 2499 NUE569 241arabidopsis|gb165|AT2G0389_T1 cloned 2500 NUE570 242arabidopsis|gb165|AT1G4391_T1 cloned 2501 NUE571 243arabidopsis|gb157.2|AT1G47530_T1 cloned 2502 NUE572 244arabidopsis|gb157.2|AT2G24240_T1 cloned 2503 NUE573 245arabidopsis|gb165|AT4G15390_T1 cloned 2504 NUE574 2556rice|gb157.2|BI807603_T1 cloned 2505 NUE575 247 rice|gb157.2|AU068829_T1cloned 2506 NUE576 2557 rice|gb157.2|AA752451_T1 cloned 2507 NUE577 249arabidopsis|gb165|AT1G6780_T1 cloned 2508 NUE578 250wheat|gb164|BE401454_T1 cloned 2509 NUE579 2558arabidopsis|gb165|AT1G7085_T1 cloned 2510 NUE580 2559arabidopsis|gb165|AT2G3588_T1 cloned 2511 NUE581 253arabidopsis|gb165|AT1G12845_T1 cloned 2512 NUE582 2560sorghum|gb161.xeno|T18303_T1 cloned 2513 NUE583 255rice|gb157.2|AU172665_T1 cloned 2514 NUE584 2561sorghum|gb161.crp|AW923545_T1 cloned 2515 NUE585 257arabidopsis|gb165|AT1G7190_T1 cloned 2516 NUE586 2562arabidopsis|gb165|AT1G7232_T1 cloned 2517 NUE587 259sorghum|gb161.xeno|AW672541_T1 cloned 2518 NUE588 260rice|gb157.2|AA750816_T1 cloned 2519 NUE590 2563sorghum|gb161.xeno|A1622209_T1 cloned 2520 NUE591 262sorghum|gb161.xeno|BE123399_T1 synthesized_ optimized 2521 NUE592 263sorghum|gb161.xeno|AI901557_T1 cloned 2522 NUE593 264arabidopsis|gb165|AT2G04066_T1 synthesized_ optimized

Table 23. Provided are the cloned or synthetically produced genes andtheir encoded polypeptides, along with the sequence identifiers,organisms from which the genes were cloned.

Example 4 Generation of Transgenic Plants Expressing the Polynucleotidesof Some Embodiments of the Invention

Arabidopsis transformation was performed according to Clough S J, Bent AF. (1998) Floral dip: a simplified method for Agrobacterium-mediatedtransformation of Arabidopsis thaliana. Plant J. 16(6): 735-43; andDesfeux C, Clough S J, Bent A F. (20000 Female reproductive tissues arethe primary targets of Agrobacterium-mediated transformation by theArabidopsis floral-dip method. Plant Physiol. 123(3): 895-904.).Briefly—Arabidopsis thaliana var Columbia (T₀ plants) were transformedusing the Floral Dip procedure described by Clough S J and Bent A F (10)and by Desfeux C et al. (11), with minor modifications. Arabidopsisthaliana Columbia (Co10) T₀ Plants were sown in 250 ml pots filled withwet peat-based growth mix. The pots were covered with aluminum foil anda plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubatedin a growth chamber at 18-24° C. under 16/8 hour light/dark cycles. TheT₀ plants were ready for transformation six days prior to anthesis.Single colonies of Agrobacterium carrying the binary vectors harboringthe polynucleotides of some embodiments of the invention were culturedin LB medium supplemented with kanamycin (50 mg/L) and gentamycin (50mg/L). The cultures were incubated at 28° C. for 48 hrs under vigorousshaking and centrifuged at 4000 rpm for 5 minutes. The pelletscomprising Agrobacterium cells were resuspended in a transformationmedium which contained half-strength (2.15 g/L) Murashige-Skoog (MS)medium (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/L B5Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSISpecialists, CT) in double-distilled water, at pH of 5.7.

Transformation of T₀ plants was performed by inverting each plant intoan Agrobacterium suspension such that the flowering stem is submergedfor 3-5 seconds. Each inoculated T₀ plant was immediately placed in aplastic tray, then covered with clear plastic dome to maintain humidityand kept in the dark at room temperature for 18 hrs to facilitateinfection and transformation. Transformed (transgenic) plants were thenuncovered and transferred to a greenhouse for recovery and maturation.The transgenic T₀ plants were grown in the greenhouse for 3-5 weeksuntil siliques maturation, and then seeds were harvested and kept atroom temperature until sowing.

For generating T1 and T₂ transgenic plants harboring the polynucleotidesof some embodiments of the invention, seeds collected from transgenic T₀plants were surface-sterilized by soaking in 70% ethanol for 1 minute,followed by soaking in 5% sodium hypochlorite and 0.05% Triton X-100 for5 minutes. The surface-sterilized seeds were thoroughly washed insterile distilled water then placed on culture plates containinghalf-strength Murashige-Skoog (Duchefa); 2% sucrose; 0.8% plant agar; 50mM kanamycin; and 200 mM carbenicylin (Duchefa). The culture plates wereincubated at 4° C. for 48 hours then transferred to a growth room at 25°C. for an additional week of incubation. Vital T₁ Arabidopsis plantswere transferred to fresh culture plates for another week of incubation.Following incubation, the T₁ plants were removed from culture plates andplanted in growth mix contained in 250 ml pots. The transgenic plantswere allowed to grow in a greenhouse to maturity. Seeds harvested fromT₁ plants were cultured and grown to maturity as T₂ plants under thesame conditions as used for culturing and growing the T₁ plants. Atleast 10 independent transformation events were created from eachconstruct for which bulk of T2 seeds were collected.

The NUE584 (SEQ ID NO: 2514), NUE253 (SEQ ID NO: 2448), NUE533 (SEQ IDNO: 2473), NUE577 (SEQ ID NO: 2507), NUE590 (SEQ ID NO: 2519) and NUE562(SEQ ID NO: 2492) genes were cloned, introduced in Arabidopsis and T2seeds were produced.

NUE540 (SEQ ID NO: 2479), NUE549 (SEQ ID NO: 2486), and NUE533 (SEQ IDNO: 2473) developed purple healthy plants, suggesting increased vigor ofthe transgenic plants.

NUE591 (SEQ ID NO: 2520) produced light green plants. This phenotyperelates the gene to the photosynthetic capacity of the plant atdifferent nitrogen fertilization levels.

Example 5 Assay 1: Improved Nitrogen Use Efficiency In Vitro (TissueCulture Assay)

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (used as a selecting agent). After sowing,plates were transferred for 2-3 days for stratification at 4° C. andthen grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7to 10 days. At this time point, seedlings randomly chosen were carefullytransferred to plates containing ½ MS media (15 mM N) for the normalnitrogen concentration treatment and 0.75 mM nitrogen for the lownitrogen concentration treatments. Each plate contained 5 seedlings ofthe same transgenic event, and 3-4 different plates (replicates) foreach event. For each polynucleotide of the invention at least fourindependent transformation events were analyzed from each construct.Plants expressing the polynucleotides of the invention were compared tothe average measurement of the control plants (empty vector or GUSreporter gene under the same promoter) used in the same experiment.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) and located in adarkroom, was used for capturing images of plantlets sawn in agarplates.

The image capturing process was repeated every 3-4 days starting at day1 till day 10 (see for example the images in FIGS. 3 A-B). An imageanalysis system was used, which consists of a personal desktop computer(Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39[Java based image processing program which was developed at the U.S.National Institutes of Health and freely available on the internet atHypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images werecaptured in resolution of 10 Mega Pixels (3888×2592 pixels) and storedin a low compression JPEG (Joint Photographic Experts Group standard)format. Next, analyzed data was saved to text files and processed usingthe JMP statistical analysis software (SAS institute).

Seedling Analysis—

Using the digital analysis seedling data was calculated, including leafarea, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following formulas V, VI and VII.Relative growth rate of leaf area=Regression coefficient of leaf areaalong time course.  Formula V:Relative growth rate of root coverage=Regression coefficient of rootcoverage along time course.  Formula VI:Relative growth rate of root length=Regression coefficient of rootcoverage along time course.  Formula VII:

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C., and weighed again to measure plant dryweight for later statistical analysis. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results were used to resolvethe effect of the gene introduced on plant vigor under optimalconditions. Similarly, the effect of the gene introduced on biomassaccumulation, under optimal conditions, was determined by comparing theplants' fresh and dry weight to that of control plants (containing anempty vector or the GUS reporter gene under the same promoter). Fromevery construct created, 3-5 independent transformation events wereexamined in replicates.

Statistical Analyses—

To identify genes conferring significantly improved plant vigor orenlarged root architecture, the results obtained from the transgenicplants were compared to those obtained from control plants. To identifyoutperforming genes and constructs, results from the independenttransformation events tested were analyzed separately. To evaluate theeffect of a gene event over a control the data was analyzed by Student'st-test and the p-value was calculated. Results were consideredsignificant if p≦0.1. The JMP statistics software package was used(Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results

The genes presented in Tables 24-25, hereinbelow, were found to improvenitrogen use efficiency (NUE) by producing larger plant biomass whengrown under limiting nitrogen growth conditions, compared to controlplants.

Tables 24 and 25 depict analyses of plant biomass (plant fresh and dryweight and leaf area) when grown under limiting nitrogen conditions [lownitrogen or nitrogen deficient conditions (0.75 mM N)] in plantsoverexpressing the polynucleotides of some embodiments of the inventionunder the regulation of a constitutive promoter (35S). Evaluation ofeach gene was performed by testing the performance of several events.Some of the genes were evaluated in more than one tissue culture assayand the second experiment confirmed the significant increment in plantbiomass. Event with p-value<0.1 was considered statisticallysignificant.

TABLE 24 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (freshand dry weight) under nitrogen deficient conditions Plant Biomass FreshPlant Biomass Dry Weight [mg] Weight [mg] Gene % Gene % Name Event #Average p-value incr. Name Event # Average p-value incr. CT11 4892.1204.50 6.3E−02 16.5 CT11 4894.3 7.80 7.7E−02 39.29 Control 175.55 CT114892.3 8.85 1.3E−01 58.04 CT22 5023.1 184.08 2.6E−04 43.5 CT11 4892.27.85 4.5E−02 40.18 Control 128.24 CT11 4893.2 5.98 6.8E−01 6.70 CT275033.7 195.00 3.2E−02 55.4 CT11 4892.1 9.20 3.7E−02 64.29 CT27 5031.4150.93 3.2E−01 20.3 Control 5.60 CT27 5035.2 233.40 2.7E−02 86.0 CT275033.7 7.68 1.2E−01 37.05 CT27 5033.6 150.63 4.2E−01 20.0 CT27 5031.46.73 2.2E−01 20.09 CT27 5033.4 179.95 1.8E−01 43.4 CT27 5035.2 9.684.3E−02 72.77 CT27 5033.8 189.30 2.0E−02 50.9 CT27 5033.6 6.40 3.1E−0114.29 CT27 5033.5 146.98 3.6E−01 17.1 CT27 5033.4 8.03 1.4E−01 43.30Control 125.47 CT27 5033.8 7.60 7.7E−02 35.71 CT6 4943.1 184.30 8.3E−0246.9 CT27 5033.5 6.40 9.6E−02 14.29 CT6 4941.4 188.38 3.0E−02 50.1Control 5.60 Control 125.47 CT6 4943.1 7.78 1.9E−01 38.84 CT76 5044.6213.08 1.8E−01 21.4 CT6 4941.4 9.60 1.3E−03 71.43 CT76 5041.5 250.501.6E−01 42.7 Control 5.60 CT76 5043.5 207.00 2.5E−01 17.9 CT76 5044.67.83 1.1E−01 22.21 CT76 5041.7 204.53 1.6E−01 16.5 CT76 5041.5 9.102.0E−01 42.12 CT76 5041.9 209.00 8.4E−02 19.1 CT76 5043.5 11.05 1.7E−0272.57 CT76 5041.6 256.10 9.0E−02 45.9 CT76 5041.7 7.35 1.9E−01 14.79Control 175.55 CT76 5041.9 7.33 2.0E−01 14.40 CT81 4992.1 223.50 1.8E−0227.3 CT76 5041.6 9.28 9.6E−02 44.85 Control 175.55 Control 6.40 NUE2088354.8 118.28 9.1E−02 17.3 CT81 4992.1 9.60 7.2E−04 49.93 NUE208 8351.3138.15 8.5E−02 37.0 CT81 4993.5 6.60 8.0E−01 3.07 NUE208 8355.3 128.534.9E−02 27.5 Control 6.40 NUE208 8351.5 112.93 3.5E−01 12.0 NUE2066731.2 7.65 8.4E−01 4.97 Control 100.81 NUE206 6732.9 10.70 7.4E−0246.83 NUE209 8191.2 135.83 4.0E−01 34.7 NUE206 6732.5 7.98 7.0E−01 9.43NUE209 8192.13 118.85 2.3E−01 17.9 Control 7.2875 NUE209 8192.14 160.401.7E−01 59.1 NUE208 8354.8 6.05 1.0E−01 55.63 NUE209 8191.5 119.281.9E−01 18.3 NUE208 8351.3 7.95 4.3E−02 104.50 NUE209 8192.1 134.454.5E−01 33.4 NUE208 8355.3 6.63 9.8E−03 70.42 Control 100.81 NUE2088351.5 6.33 1.0E−02 62.70 NUE211 8265.1 118.48 6.7E−02 47.0 Control 3.89Control 80.58 NUE209 8191.2 6.20 2.2E−01 59.49 NUE212 8331.1 115.184.7E−01 14.2 NUE209 8192.13 5.80 4.4E−02 49.20 NUE212 8335.2 182.986.6E−03 81.5 NUE209 8192.14 7.58 6.3E−02 94.86 NUE212 8334.1 121.252.6E−01 20.3 NUE209 8191.5 7.85 5.2E−02 101.93 NUE212 8331.4 177.681.1E−01 76.2 NUE209 8192.1 5.85 9.5E−02 50.48 Control 100.81 Control3.89 NUE221 9802.8 134.30 1.6E−01 18.0 NUE210 8202.1 4.83 4.9E−01 24.12NUE221 9806.1 138.60 9.0E−02 21.8 NUE210 8202.2 6.35 6.3E−03 63.34Control 113.81 NUE210 8201.3 5.50 4.7E−02 41.48 NUE222 8851.4 131.685.8E−03 44.1 Control 3.89 NUE222 8854.1 108.37 6.9E−02 18.6 NUE2128334.1 5.18 2.2E−01 42.76 NUE222 8853.2 119.23 1.9E−02 30.4 NUE2128332.1 3.83 7.5E−01 5.52 NUE222 8851.3 161.75 2.1E−02 77.0 NUE212 8331.44.75 6.6E−02 31.03 Control 91.40 Control 3.63 NUE227 9851.2 102.751.2E−01 26.1 NUE221 9806.1 6.025 1.9E−01 18.7 NUE227 9854.2 84.708.3E−01 4.0 Control 5.075 NUE227 9853.4 102.83 2.9E−01 26.2 NUE2228851.4 4.03 7.9E−01 3.54 NUE227 9853.1 103.23 1.3E−01 26.7 NUE222 8853.24.65 2.2E−01 19.61 NUE227 9852.3 101.98 1.4E−01 25.2 NUE222 8851.3 6.453.3E−03 65.92 Control 81.48 Control 3.89 NUE230 9154.2 181.58 2.3E−0248.8 NUE224 9002.2 6.93 2.1E−02 39.55 NUE230 9151.2 125.18 8.8E−01 2.6NUE224 9001.3 6.83 2.8E−01 37.70 Control 122.05 Control 4.96 NUE23110633.3 138.98 8.5E−02 22.1 NUE227 9851.2 4.90 3.6E−01 24.05 Control113.81 NUE227 9854.2 4.60 4.8E−01 16.46 NUE233 10174.3 156.40 3.7E−0260.8 NUE227 9853.4 4.55 5.3E−01 15.19 NUE233 10174.1 176.20 4.0E−03 81.2NUE227 9853.1 4.83 1.4E−01 22.15 NUE233 10173.7 103.68 7.4E−01 6.6NUE227 9852.3 5.18 7.7E−02 31.01 Control 97.24 Control 3.95 NUE23310174.1 117.95 8.1E−03 37.2 NUE228 10092.2 6.75 2.8E−02 35.34 NUE23310173.7 95.40 4.6E−01 10.9 Control 4.99 Control 86.00 NUE230 9154.2 7.832.2E−02 57.68 NUE235 9691.1 175.50 3.2E−01 43.8 NUE230 9151.2 5.405.4E−01 8.82 NUE235 9693.3 178.60 3.1E−03 46.3 NUE230 9153.3 5.286.9E−01 6.30 NUE235 9694.3 156.28 2.1E−01 28.0 NUE230 9153.1 5.483.3E−01 10.33 Control 122.05 Control 4.96 NUE237 9651.1 159.43 1.6E−0130.6 NUE231 10633.3 8.425 1.0E−05 66.0 NUE237 9654.4 170.70 4.1E−02 39.9Control 5.075 NUE237 9654.1 128.70 7.1E−01 5.4 NUE233 10174.3 6.055.1E−02 44.05 NUE237 9653.3 133.33 6.1E−01 9.2 NUE233 10174.1 8.152.7E−03 94.05 Control 122.05 NUE233 10173.7 4.45 7.3E−01 5.95 NUE2399192.3 168.58 5.1E−02 27.4 Control 4.20 NUE239 9192.1 142.68 1.7E−01 7.8NUE235 9694.2 5.25 7.9E−01 5.79 NUE239 9191.2 136.75 5.8E−01 3.3 NUE2359691.1 7.28 1.6E−01 46.60 Control 132.34 NUE235 9694.4 5.83 4.1E−0117.38 NUE240 9172.1 157.53 5.3E−03 19.0 NUE235 9693.3 7.28 6.0E−02 46.60NUE240 9174.3 143.65 1.7E−01 8.5 NUE235 9694.3 7.88 1.5E−02 58.69Control 132.34 Control 4.96 NUE241 9632.5 133.63 1.4E−01 64.0 NUE2379651.1 7.03 3.2E−01 41.56 NUE241 9631.3 148.18 3.8E−03 81.9 NUE2379654.4 8.88 2.0E−02 78.84 NUE241 9632.3 137.60 8.0E−03 68.9 NUE2379654.1 6.23 6.5E−02 25.44 NUE241 9632.4 131.93 1.9E−02 61.9 NUE2379653.3 7.08 8.5E−02 42.57 Control 81.48 Control 4.96 NUE242 9212.1124.50 2.7E−02 48.7 NUE239 9191.1 9.00 5.3E−02 60.71 NUE242 9214.1 89.987.5E−01 7.4 NUE239 9191.2 6.13 5.2E−01 9.38 NUE242 9211.2 95.30 4.3E−0113.8 Control 5.60 NUE242 9213.4 125.50 2.8E−02 49.9 NUE240 9172.4 7.353.5E−02 18.55 Control 83.75 NUE240 9174.3 6.53 6.1E−01 5.24 NUE2449061.1 119.60 2.8E−03 30.9 Control 6.20 NUE244 9061.5 148.80 1.4E−0162.8 NUE241 9633.4 5.68 6.1E−02 43.67 Control 91.40 NUE241 9632.3 6.551.3E−02 65.82 NUE246 9033.6 140.75 1.4E−01 41.2 NUE241 9632.2 6.204.4E−02 56.96 NUE246 9033.8 111.20 4.8E−01 11.6 NUE241 9632.4 5.687.9E−04 43.67 NUE246 9033.4 148.63 2.7E−03 49.1 Control 3.95 NUE2469034.1 138.50 6.0E−03 39.0 NUE246 9033.6 4.40 3.2E−01 19.32 NUE2469031.1 131.63 3.5E−01 32.1 NUE246 9033.8 5.13 1.8E−01 38.98 Control99.68 NUE246 9033.4 4.73 8.2E−02 28.14 NUE248 8981.5 197.35 1.3E−02 30.1NUE246 9034.1 6.43 1.7E−02 74.46 Control 151.66 NUE246 9031.1 4.802.5E−01 30.17 NUE249 9124.2 82.68 9.2E−01 3.4 Control 3.69 NUE249 9121.4125.13 2.0E−03 56.5 NUE248 8981.5 7.98 6.0E−02 23.17 NUE249 9123.3 89.552.3E−01 12.0 NUE248 8984.1 6.75 7.1E−01 4.25 Control 79.94 NUE248 8981.27.35 3.3E−01 13.51 NUE250 9132.1 149.53 4.6E−02 22.5 Control 6.48 NUE2509133.2 193.20 1.3E−02 58.3 NUE249 9124.2 5.05 7.6E−01 11.60 NUE2509132.2 152.38 1.8E−01 24.8 NUE249 9121.4 5.50 2.3E−02 21.55 NUE2509134.1 205.50 5.0E−02 68.4 Control 4.53 Control 122.05 NUE250 9132.17.38 4.8E−02 48.61 NUE251 10181.3 175.35 4.7E−03 80.3 NUE250 9133.2 7.952.5E−03 60.20 NUE251 10183.2 127.58 1.7E−01 31.2 NUE250 9132.2 7.231.0E−01 45.59 NUE251 10183.1 118.03 3.6E−01 21.4 NUE250 9134.1 8.031.1E−02 61.71 Control 97.24 Control 4.96 NUE252 9011.3 252.23 1.3E−0240.9 NUE251 10181.3 7.03 2.9E−02 67.26 NUE252 9012.2 201.53 3.1E−01 12.5NUE251 10183.2 5.35 2.6E−01 27.38 Control 179.06 NUE251 10183.1 4.934.6E−01 17.26 NUE256 10063.4 127.55 1.7E−01 31.2 Control 4.2 NUE25610064.1 155.88 3.1E−02 60.3 NUE256 10063.4 5.78 1.0E−01 37.50 NUE25610061.2 158.93 1.1E−02 63.4 NUE256 10064.1 7.40 1.0E−02 76.19 NUE25610062.4 147.40 1.4E−01 51.6 NUE256 10061.2 6.35 1.8E−02 51.19 NUE25610063.2 134.18 1.9E−01 38.0 NUE256 10062.4 7.43 1.4E−01 76.79 NUE25610061.1 101.35 8.3E−01 4.2 NUE256 10063.2 5.73 1.4E−01 36.31 Control97.24 NUE256 10061.1 4.40 8.0E−01 4.76 NUE256 10061.2 104.20 5.5E−0121.2 Control 4.20 NUE256 10061.4 127.68 1.8E−01 48.5 NUE512 9284.3 4.656.8E−01 5.38 NUE256 10063.2 88.28 8.5E−01 2.6 NUE512 9282.3 7.78 1.3E−0276.20 Control 86.00 NUE512 9284.4 6.70 2.6E−02 51.84 NUE268 8992.1108.25 4.3E−01 10.4 Control 4.41 NUE268 8996.3 118.68 5.5E−01 21.0NUE515 9713.6 6.725 2.4E−02 32.5 NUE268 8996.5 177.25 8.4E−02 80.8Control 5.075 NUE268 8996.2 112.10 3.7E−01 14.3 NUE516 9291.1 5.782.4E−01 16.37 Control 98.05 NUE516 9291.4 7.43 2.7E−02 49.62 NUE2699104.1 93.80 3.0E−01 23.1 NUE516 9293.2 5.78 4.3E−01 16.37 NUE269 9101.394.65 6.2E−03 24.2 Control 4.96 Control 76.20 NUE519 9371.2 11.754.8E−02 89.52 NUE512 9284.2 166.53 6.1E−05 73.7 NUE519 9371.1 8.501.5E−02 37.10 NUE512 9284.3 112.35 2.1E−01 17.2 NUE519 9372.2 6.606.7E−01 6.45 NUE512 9282.3 181.10 1.3E−02 88.9 Control 6.20 NUE5129284.4 171.70 7.6E−02 79.1 NUE525 9531.2 5.25 3.6E−01 −10.45 Control95.88 NUE525 9534.1 5.40 4.4E−01 −7.89 NUE515 9712.5 116.43 8.6E−01 2.3NUE525 9531.3 6.73 4.4E−01 14.71 NUE515 9713.6 148.18 2.0E−02 30.2NUE525 9533.1 7.43 1.2E−02 26.65 Control 113.81 NUE525 9531.1 7.209.9E−02 22.81 NUE514 9404.1 113.98 8.5E−02 36.1 Control 5.86 NUE5149403.2 94.58 1.2E−01 12.9 NUE531 10083.1 6.58 4.2E−02 31.83 NUE5149402.5 99.38 3.5E−01 18.7 NUE531 10082.2 6.25 1.6E−01 25.31 Control83.75 NUE531 10081.4 8.50 2.4E−02 70.43 NUE516 9291.1 128.58 6.7E−01 5.3NUE531 10081.5 8.03 2.5E−02 60.90 NUE516 9291.4 165.93 1.5E−01 35.9Control 4.99 NUE516 9293.2 139.73 5.7E−01 14.5 NUE532 9222.4 6.153.5E−05 44.71 Control 122.05 NUE532 9222.1 6.98 1.3E−02 64.12 NUE5199371.2 182.10 1.5E−02 37.6 NUE532 9223.3 5.53 1.4E−01 30.00 Control132.34 NUE532 9224.4 4.43 4.4E−01 4.12 NUE521 9363.1 107.40 3.1E−01 28.2Control 4.25 NUE521 9362.2 119.80 2.6E−02 43.0 NUE536 9233.3 6.034.8E−03 63.39 NUE521 9361.2 136.10 3.5E−04 62.5 NUE536 9234.1 4.553.3E−01 23.39 NUE521 9361.3 104.15 3.8E−01 24.4 NUE536 9231.3 4.085.7E−01 10.51 NUE521 9363.4 132.95 5.8E−03 58.7 NUE536 9232.4 3.986.1E−01 7.80 Control 83.75 Control 3.69 NUE523 9412.5 190.08 2.4E−0125.3 NUE537 9391.1 5.08 3.5E−01 15.01 NUE523 9414.2 192.23 1.9E−01 26.7NUE537 9393.2 4.53 9.2E−01 2.55 NUE523 9412.1 187.50 2.8E−02 23.6 NUE5379394.4 5.90 2.8E−01 33.71 Control 151.66 NUE537 9391.2 5.53 2.2E−0125.21 NUE527 9201.1 111.28 3.2E−03 273.7 NUE537 9393.3 5.63 2.7E−0227.48 NUE527 9202.6 51.70 2.1E−02 73.6 Control 4.41 NUE527 9203.2 49.774.3E−02 67.1 NUE539 10101.5 6.78 4.4E−03 61.31 NUE527 9204.1 45.833.0E−01 53.9 NUE539 10103.5 5.63 1.7E−01 33.93 Control 29.78 NUE53910101.2 7.43 4.4E−02 76.79 NUE531 10083.1 159.05 9.2E−02 38.1 NUE53910101.7 7.05 2.3E−02 67.86 NUE531 10082.2 154.43 1.6E−02 34.1 NUE53910103.4 4.88 3.6E−01 16.07 NUE531 10081.4 173.70 5.9E−02 50.8 Control4.20 NUE531 10081.5 154.38 1.9E−02 34.0 NUE542 9333.2 8.35 2.6E−02 89.24Control 115.16 NUE542 9334.1 4.80 5.2E−01 8.78 NUE531 10081.4 128.203.4E−02 49.1 NUE542 9331.3 4.83 6.9E−01 9.35 NUE531 10083.2 91.956.4E−01 6.9 NUE542 9334.3 4.65 8.1E−01 5.38 NUE531 10081.5 165.435.5E−02 92.4 Control 4.41 Control 86.00 NUE548 9095.2 8.05 2.6E−01 44.07NUE532 9222.4 143.08 9.9E−02 70.8 NUE548 9095.4 8.60 4.7E−02 53.91NUE532 9222.1 106.98 2.3E−01 27.7 NUE548 9091.1 6.43 3.0E−01 14.99NUE532 9223.3 100.20 1.3E−01 19.6 Control 5.59 Control 83.75 NUE5499343.7 7.67 2.2E−02 54.88 NUE532 9222.4 118.33 7.6E−02 41.3 Control 4.95NUE532 9222.1 170.88 1.1E−01 104.0 NUE550 9143.1 5.75 3.9E−04 35.29NUE532 9223.3 115.80 3.1E−01 38.3 NUE550 9143.4 6.85 7.3E−03 61.18NUE532 9223.5 104.90 7.7E−01 25.3 NUE550 9142.2 6.45 1.6E−02 51.76NUE532 9224.4 110.48 6.3E−01 31.9 Control 4.25 Control 98.05 NUE5539181.5 5.55 7.6E−02 30.59 NUE535 9082.2 32.95 4.7E−01 10.7 NUE553 9184.34.58 6.3E−01 7.65 NUE535 9086.2 73.97 3.2E−02 148.4 NUE553 9182.2 4.702.4E−01 10.59 NUE535 9086.3 51.43 6.0E−02 72.7 Control 4.25 NUE5359081.1 61.90 1.9E−01 107.9 NUE554 9114.1 4.33 9.4E−01 1.76 NUE535 9084.457.00 9.3E−03 91.4 NUE554 9115.2 6.88 1.3E−02 61.76 Control 29.78 NUE5549114.2 5.35 1.8E−02 25.88 NUE537 9391.1 131.75 2.2E−01 37.4 NUE5549115.3 4.45 5.9E−01 4.71 NUE537 9393.2 110.88 3.9E−01 15.6 Control 4.25NUE537 9394.4 214.60 6.4E−02 123.8 NUE564 9242.3 4.55 6.7E−05 114.12NUE537 9391.2 141.33 3.2E−02 47.4 NUE564 9243.2 4.03 7.8E−02 89.41NUE537 9393.3 136.40 2.1E−04 42.3 NUE564 9242.4 3.28 3.6E−02 54.12Control 95.88 NUE564 9242.2 3.90 7.6E−02 83.53 NUE538 9782.4 108.852.7E−01 33.6 NUE564 9243.4 4.35 2.2E−06 104.71 NUE538 9781.4 95.734.5E−01 17.5 Control 2.13 NUE538 9781.1 94.65 2.6E−01 16.2 NUE567 9263.23.15 2.1E−01 48.24 NUE538 9782.1 145.73 8.8E−02 78.9 NUE567 9261.3 3.051.8E−02 43.53 Control 81.48 NUE567 9263.3 3.28 8.5E−03 54.12 NUE53910101.5 163.80 2.4E−02 68.5 NUE567 9261.4 3.28 5.9E−03 54.12 NUE53910103.5 124.98 2.8E−01 28.5 Control 2.13 NUE539 10101.2 177.98 2.7E−0283.0 NUE569 9384.4 2.63 3.0E−01 23.53 NUE539 10101.7 162.73 2.5E−02 67.3NUE569 9381.2 5.20 1.8E−02 144.71 NUE539 10103.4 105.13 7.6E−01 8.1NUE569 9381.5 2.90 5.3E−01 36.47 Control 97.24 NUE569 9381.3 4.531.3E−01 112.94 NUE542 9333.2 165.80 6.0E−02 72.9 NUE569 9384.2 3.584.5E−01 68.24 NUE542 9331.3 150.08 9.7E−02 56.5 Control 2.13 NUE5429334.3 153.73 2.9E−03 60.3 NUE570 9311.4 4.23 1.6E−01 98.82 NUE5429332.1 167.08 1.0E−01 74.3 NUE570 9313.3 3.85 5.1E−02 81.18 Control95.88 NUE570 9314.4 3.58 8.1E−01 68.24 NUE542 9333.2 169.30 1.0E−01 38.7NUE570 9314.1 4.25 4.3E−02 100.00 NUE542 9332.1 165.13 3.6E−02 35.3NUE570 9312.3 4.33 4.8E−01 103.53 Control 122.05 Control 2.13 NUE54310051.2 99.90 1.4E−01 22.6 NUE571 9304.2 4.28 1.1E−02 101.18 NUE54310051.6 113.23 1.8E−01 39.0 NUE571 9304.3 4.15 1.7E−02 95.29 NUE54310053.1 97.10 3.1E−01 19.2 NUE571 9303.2 5.13 6.8E−05 141.18 NUE54310054.2 121.08 9.3E−03 48.6 NUE571 9302.3 3.63 7.7E−02 70.59 Control81.48 NUE571 9301.4 3.90 1.9E−02 83.53 NUE544 9764.1 143.68 3.0E−02 33.9Control 2.13 NUE544 9763.4 115.60 4.9E−01 7.7 NUE572 9321.3 3.35 2.6E−0257.65 NUE544 9764.2 127.50 1.6E−01 18.8 NUE572 9321.1 4.50 2.2E−02111.76 NUE544 9763.3 123.08 3.5E−02 14.7 NUE572 9322.1 3.03 1.2E−0142.35 Control 107.29 NUE572 9324.3 4.10 1.5E−02 92.94 NUE549 9343.7142.03 1.9E−02 19.6 NUE572 9322.2 3.63 1.2E−02 70.59 Control 118.75Control 2.13 NUE550 9141.3 109.05 5.0E−01 11.2 NUE573 9491.4 5.653.2E−01 14.14 NUE550 9143.1 136.53 1.7E−01 39.2 NUE573 9491.1 6.181.2E−01 24.75 NUE550 9143.4 161.73 1.3E−02 64.9 NUE573 9493.2 5.533.2E−01 11.78 NUE550 9142.2 131.30 5.7E−02 33.9 Control 4.95 Control98.05 NUE574 10364.2 5.73 2.9E−01 11.98 NUE553 9181.5 52.48 2.2E−04 76.2NUE574 10366.2 7.08 5.3E−02 38.39 NUE553 9184.1 57.30 3.2E−03 92.4Control 5.11 NUE553 9184.3 82.23 1.3E−05 176.2 NUE576 9791.3 6.708.6E−04 69.62 NUE553 9185.2 56.35 8.3E−04 89.3 NUE576 9792.4 5.186.6E−02 31.01 NUE553 9182.2 68.93 8.8E−03 131.5 NUE576 9794.1 4.784.0E−01 20.89 Control 29.78 NUE576 9793.3 5.65 8.4E−04 43.04 NUE5549115.2 215.73 3.9E−02 120.0 Control 3.95 NUE554 9114.2 116.98 1.9E−0119.3 NUE581 9723.6 5.875 2.7E−01 15.8 Control 98.05 NUE581 9724.9 5.4256.3E−01 6.9 NUE564 9242.3 120.53 8.6E−03 25.7 Control 5.075 NUE5649243.2 130.18 2.3E−02 35.8 NUE582 9564.2 5.28 5.6E−01 6.30 NUE564 9242.2221.87 6.4E−02 131.4 NUE582 9562.4 6.45 1.8E−01 29.97 NUE564 9243.4121.85 1.0E−01 27.1 NUE582 9561.2 6.95 1.4E−03 40.05 Control 95.88Control 4.96 NUE567 9263.2 133.50 8.5E−02 39.2 NUE583 9673.1 5.801.1E−01 46.84 NUE567 9261.2 133.75 2.6E−01 39.5 NUE583 9673.2 4.332.7E−01 9.49 Control 95.88 NUE583 9671.2 5.28 2.1E−01 33.54 NUE5689461.2 164.90 7.0E−02 38.9 NUE583 9671.1 4.88 3.7E−01 23.42 Control118.75 Control 3.95 NUE569 9381.2 97.40 1.5E−01 20.5 NUE585 9662.4 4.653.3E−01 26.10 NUE569 9381.3 108.85 7.3E−02 34.7 NUE585 9661.5 4.785.4E−02 29.49 NUE569 9384.2 93.95 9.3E−02 16.2 NUE585 9661.3 3.906.6E−01 5.76 Control 80.83 NUE585 9662.1 4.00 6.9E−01 8.47 NUE570 9311.4127.53 1.3E−01 33.0 NUE585 9661.1 4.43 2.5E−01 20.00 NUE570 9314.1135.55 3.8E−01 41.4 Control 3.69 NUE570 9312.3 143.83 3.5E−02 50.0NUE586 9751.1 5.38 1.9E−01 22.86 Control 95.88 NUE586 9751.7 6.431.4E−01 46.86 NUE573 9491.1 135.18 6.0E−02 13.8 NUE586 9752.1 8.153.7E−04 86.29 Control 118.75 Control 4.38 NUE574 10364.2 110.48 2.1E−0228.5 NUE587 9643.2 7.45 8.6E−03 70.29 NUE574 10362. 2 89.95 7.8E−01 4.6NUE587 9643.1 5.58 9.3E−02 27.43 NUE574 10366.2 153.38 8.1E−02 78.3NUE587 9642.2 4.50 8.7E−01 2.86 Control 86.00 NUE587 9641.3 6.88 4.2E−0257.14 NUE576 9791.3 164.75 1.6E−02 102.2 Control 4.38 NUE576 9792.390.20 4.8E−01 10.7 NUE588 9591.3 5.03 5.9E−02 36.27 NUE576 9792.4 106.401.0E−01 30.6 NUE588 9591.4 3.75 9.4E−01 1.69 NUE576 9794.1 108.431.9E−01 33.1 NUE588 9592.2 4.68 3.7E−01 26.78 NUE576 9793.3 136.936.6E−02 68.1 NUE588 9592.4 4.98 1.7E−01 34.92 Control 81.48 NUE5889592.1 3.98 7.2E−01 7.80 NUE583 9673.4 171.65 5.2E−03 99.6 Control 3.69NUE583 9673.2 117.30 3.2E−03 36.4 NUE592 9744.5 9.000 3.7E−07 77.3Control 86.00 NUE592 9747.5 7.900 2.1E−05 55.7 NUE585 9662.4 135.601.7E−01 36.0 Control 5.075 NUE585 9661.5 122.20 6.2E−02 22.6 Control99.68 NUE586 9751.1 153.83 2.1E−01 88.8 NUE586 9752.1 194.23 2.2E−02138.4 Control 129.73 NUE587 9643.2 156.78 2.0E−01 92.4 NUE587 9641.3180.28 6.6E−02 121.3 Control 129.73 NUE592 9744.5 187.48 1.8E−06 64.7NUE592 9747.5 155.45 5.0E−03 36.6 Control 113.81

Table 24: Analyses of plant biomass (plant fresh and dry weight) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under limiting nitrogen conditions [low nitrogen or nitrogendeficient conditions (0.75 mM N)] as compared to control plants.“Incr.”=increment.

TABLE 25 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (leafarea) under nitrogen deficient conditions Leaf Area [cm²] Gene NameEvent # Average p-value % increment CT11 4894.3 0.70 4.9E−02 43.10 CT114892.3 0.77 4.0E−02 57.27 CT11 4892.2 0.65 7.4E−02 33.68 CT11 4893.20.51 6.3E−01 4.12 CT11 4892.1 0.93 1.2E−02 91.25 Control 0.49 CT275033.7 0.40 8.2E−01 3.15 CT27 5031.4 0.59 7.3E−04 50.88 CT27 5035.2 0.529.6E−03 33.43 CT27 5033.4 0.45 5.2E−01 14.02 Control 0.39 CT6 4943.10.58 1.1E−01 47.28 CT6 4941.4 0.56 6.0E−02 43.93 Control 0.39 CT765044.6 0.58 2.9E−01 19.76 CT76 5041.5 0.74 1.1E−01 50.86 CT76 5043.50.81 2.8E−04 66.21 CT76 5041.9 0.79 5.4E−02 61.76 CT76 5041.6 0.807.2E−03 64.90 Control 0.49 CT81 4992.1 0.70 3.0E−04 44.35 CT81 4993.60.50 8.2E−01 3.08 CT81 4993.5 0.59 3.2E−01 20.36 CT81 4992.2 0.698.9E−02 41.46 Control 0.49 NUE206 6731.2 0.34 1.4E−02 30.90 NUE2066732.7 0.30 1.9E−01 15.55 Control 0.26 NUE208 8354.8 0.33 4.7E−01 8.19NUE208 8351.3 0.43 5.8E−02 44.03 NUE208 8355.3 0.37 7.4E−02 22.98 NUE2088351.5 0.31 7.3E−01 3.72 Control 0.30 NUE209 8192.13 0.39 1.6E−01 30.67NUE209 8192.14 0.47 5.9E−02 56.92 NUE209 8191.5 0.32 6.9E−01 4.74Control 0.30 NUE209 8192.13 0.45 4.7E−03 34.55 NUE209 8191.5 0.512.8E−02 53.15 NUE209 8192.14 0.40 1.5E−01 20.45 Control 0.33 NUE2118265.1 0.35 7.9E−02 17.46 Control 0.30 NUE212 8335.2 0.43 1.7E−03 43.62NUE212 8334.1 0.33 5.3E−01 10.76 NUE212 8331.4 0.44 3.5E−02 44.45Control 0.30 NUE221 9801.1 0.500 1.0E−01 20.5 NUE221 9802.8 0.5058.2E−02 21.8 NUE221 9806.1 0.666 4.2E−06 60.5 Control 0.415 NUE2249001.3 0.62 1.7E−03 38.99 Control 0.44 NUE225 9732.8 0.445 5.6E−01 7.2NUE225 9734.5 0.484 1.8E−01 16.6 NUE225 9734.9 0.439 6.4E−01 5.7 Control0.415 NUE230 9154.2 0.52 7.3E−02 17.73 NUE230 9151.2 0.50 1.2E−01 12.59Control 0.44 NUE231 10633.3 0.573 2.8E−04 38.1 Control 0.415 NUE23310174.3 0.47 8.4E−03 54.39 NUE233 10174.1 0.75 2.8E−04 146.46 NUE23310172.5 0.32 6.6E−01 6.03 NUE233 10173.7 0.39 3.5E−02 26.43 Control 0.31NUE237 9651.1 0.52 4.4E−01 17.13 NUE237 9654.4 0.57 1.1E−01 29.19 NUE2379654.1 0.54 2.2E−02 21.91 Control 0.44 NUE239 9191.1 0.66 4.6E−02 57.34Control 0.42 NUE240 9172.2 0.68 1.4E−03 63.63 NUE240 9174.3 0.55 7.7E−0232.26 Control 0.42 NUE240 9174.2 0.54 7.7E−01 4.29 NUE240 9172.1 0.717.8E−03 35.86 NUE240 9174.3 0.57 4.2E−01 9.74 Control 0.52 NUE241 9633.40.56 2.1E−05 47.36 NUE241 9632.2 0.44 1.4E−01 15.74 NUE241 9632.4 0.492.4E−01 28.11 Control 0.38 NUE241 9631.3 0.53 3.9E−03 36.03 NUE2419632.3 0.54 7.5E−02 40.72 NUE241 9632.4 0.49 1.1E−01 26.49 Control 0.39NUE242 9212.1 0.56 2.9E−02 50.18 NUE242 9213.4 0.43 1.3E−01 14.72Control 0.37 NUE246 9033.6 0.44 8.7E−01 3.16 NUE246 9033.8 0.51 1.8E−0121.26 NUE246 9033.4 0.50 2.6E−01 18.30 NUE246 9034.1 0.64 2.1E−02 52.45NUE246 9031.1 0.49 4.7E−01 15.11 Control 0.42 NUE248 8981.5 0.58 4.6E−0238.97 NUE248 8981.2 0.51 3.1E−02 22.19 Control 0.42 NUE251 10181.3 0.547.6E−06 75.63 NUE251 10183.2 0.42 2.3E−02 38.78 NUE251 10183.1 0.425.4E−03 38.58 Control 0.31 NUE251 10183.2 0.84 1.0E−02 72.56 NUE25110182.1 0.57 1.2E−01 18.26 Control 0.49 NUE256 10063.4 0.43 6.3E−01 5.53NUE256 10064.1 0.54 1.3E−02 30.76 NUE256 10061.1 0.45 5.2E−01 10.87Control 0.41 NUE256 10063.4 0.58 6.6E−02 90.27 NUE256 10064.1 0.591.1E−02 94.09 NUE256 10061.2 0.68 9.5E−06 122.00 NUE256 10062.4 0.648.2E−03 108.97 NUE256 10063.2 0.52 9.2E−03 68.33 Control 0.31 NUE25610061.2 0.75 5.3E−05 53.75 NUE256 10061.4 0.71 1.4E−02 46.02 NUE25610063.2 0.52 4.1E−01 6.83 Control 0.49 NUE268 8996.5 0.65 4.3E−02 21.87Control 0.53 NUE511 9273.1 0.47 4.7E−01 13.04 NUE511 9271.2 0.54 2.2E−0228.41 Control 0.42 NUE512 9282.3 0.72 2.1E−02 68.37 NUE512 9284.4 0.607.8E−03 39.86 Control 0.43 NUE514 9404.1 0.49 1.1E−02 30.78 NUE5149402.2 0.41 5.1E−01 10.03 NUE514 9403.2 0.42 1.5E−01 12.66 NUE514 9402.50.42 5.1E−01 13.45 Control 0.37 NUE515 9712.5 0.454 4.5E−01 9.4 NUE5159713.6 0.648 1.7E−05 56.1 Control 0.415 NUE520 9771.4 0.40 3.1E−03 31.70Control 0.31 NUE521 9362.2 0.50 7.5E−02 33.38 NUE521 9361.2 0.43 1.6E−0114.92 NUE521 9363.4 0.57 1.3E−03 54.08 Control 0.37 NUE521 9363.4 0.638.6E−02 31.31 Control 0.48 NUE523 9412.5 0.59 1.9E−01 40.65 NUE5239414.2 0.48 1.6E−01 16.08 NUE523 9412.1 0.65 7.4E−03 55.07 Control 0.42NUE525 9531.2 0.44 2.9E−01 15.42 NUE525 9534.1 0.45 3.8E−01 17.35 NUE5259531.3 0.51 6.2E−02 34.94 NUE525 9533.1 0.54 6.9E−02 42.25 NUE525 9531.10.49 2.7E−03 29.15 Control 0.38 NUE527 9201.1 0.44 6.7E−02 22.51 Control0.36 NUE528 9072.1 0.48 4.5E−02 17.39 NUE528 9073.1 0.42 7.8E−01 3.17Control 0.41 NUE531 10083.1 0.74 2.0E−02 31.74 NUE531 10082.2 0.693.0E−02 22.95 NUE531 10081.4 0.75 8.8E−02 32.91 NUE531 10081.5 0.751.3E−01 33.71 Control 0.56 NUE535 9082.2 0.37 4.1E−01 22.10 NUE5359084.2 0.37 4.6E−02 22.19 NUE535 9081.1 0.43 1.7E−01 41.23 NUE535 9083.10.63 2.8E−03 105.40 NUE535 9084.4 0.46 8.7E−03 50.93 Control 0.31 NUE5379391.2 0.65 1.6E−02 51.35 NUE537 9393.3 0.76 3.4E−03 76.76 Control 0.43NUE539 10101.5 0.53 1.2E−02 74.32 NUE539 10103.5 0.50 6.6E−05 63.28NUE539 10101.2 0.60 7.3E−05 96.27 NUE539 10101.7 0.68 3.9E−03 121.77NUE539 10103.4 0.32 8.9E−01 4.57 Control 0.31 NUE542 9333.2 0.56 2.0E−0225.55 Control 0.44 NUE543 10051.2 0.43 4.6E−01 11.78 NUE543 10051.6 0.521.7E−02 33.53 Control 0.39 NUE544 9764.2 0.49 9.7E−02 19.20 Control 0.41NUE548 9095.2 0.59 3.4E−03 41.28 NUE548 9095.3 0.47 3.1E−01 13.55 NUE5489092.2 0.55 1.8E−01 32.59 Control 0.42 NUE548 9095.2 0.67 3.3E−01 10.81NUE548 9095.4 0.82 5.7E−02 36.33 NUE548 9091.1 0.71 2.4E−02 17.16Control 0.60 NUE568 9471.3 0.54 3.5E−02 18.92 NUE568 9472.2 0.64 3.6E−0441.93 Control 0.45 NUE573 9491.4 0.59 6.8E−02 29.98 NUE573 9491.1 0.516.1E−01 13.35 NUE573 9494.3 0.52 2.8E−01 14.77 Control 0.45 NUE57410364.2 0.63 8.1E−04 28.96 NUE574 10366.2 0.81 4.0E−03 66.05 Control0.49 NUE576 9791.3 0.47 8.9E−02 21.80 NUE576 9792.3 0.39 9.8E−01 0.42NUE576 9792.4 0.48 1.5E−01 24.25 NUE576 9794.1 0.46 1.3E−01 19.07 NUE5769793.3 0.49 6.6E−02 25.95 Control 0.39 NUE581 9723.6 0.449 5.0E−01 8.3NUE581 9724.9 0.583 1.5E−03 40.6 Control 0.415 NUE582 9562.4 0.547.0E−02 21.31 NUE582 9561.2 0.51 6.2E−02 14.15 Control 0.44 NUE5839673.1 0.56 6.5E−02 46.13 Control 0.39 NUE583 9673.4 0.91 2.1E−02 88.22NUE583 9673.2 0.65 8.8E−02 34.45 Control 0.49 NUE586 9751.7 0.52 3.8E−0111.42 NUE586 9752.1 0.62 1.1E−02 31.98 Control 0.47 NUE586 9751.7 0.502.3E−01 23.80 NUE586 9751.3 0.42 6.9E−01 3.28 NUE586 9752.4 0.53 3.2E−0230.20 NUE586 9752.1 0.53 2.9E−01 30.75 Control 0.41 NUE587 9643.2 0.581.5E−02 24.86 Control 0.47 NUE592 9741.7 0.462 3.7E−01 11.3 NUE5929744.5 0.721 4.3E−08 73.7 NUE592 9747.4 0.472 2.7E−01 13.8 NUE592 9747.50.711 1.0E−07 71.4 Control

Table 25: Analyses of plant biomass (leaf area) of transgenic plantsoverexpressing the exogenous polynucleotides of some embodiments of theinvention (using the cloned or synthetic genes listed in Table 23 above)under the regulation of a constitutive promoter (35S) when grown underlimiting nitrogen conditions [low nitrogen or nitrogen deficientconditions (0.75 mM N)] as compared to control plants.

The genes presented in Table 26, hereinbelow, have improved plant NUEsince they produced larger root biomass when grown under limitingnitrogen growth conditions, compared to control plants. Plants producinglarger root biomass have better possibilities to absorb larger amount ofnitrogen from soil.

Table 26 depicts analyses of root biomass (root length and rootcoverage) when grown under limiting nitrogen conditions [low nitrogen ornitrogen deficient conditions (0.75 mM N)] in plants overexpressing thepolynucleotides of some embodiments of the invention under theregulation of a constitutive promoter (35S). Evaluation of each gene wasperformed by testing the performance of several events. Some of thegenes were evaluated in more than one tissue culture assay and thesecond experiment confirmed the significant increment in rootperformance. Event with p-value<0.1 was considered statisticallysignificant.

TABLE 26 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved root performanceunder nitrogen deficient conditions Roots Length [cm] Roots Coverage[cm²] Gene Event % Gene Event % Name # Ave. p-value incr. Name # Ave.p-value incr. CT1 4844.5 3.445 1.1E−01 19.68 CT11 4894.3 8.833 4.1E−0116.49 CT1 4841.2 3.580 2.6E−01 24.38 CT11 4892.1 8.345 6.3E−01 10.06Control 2.879 CT11 4892.1 9.792 8.1E−02 29.14 CT27 5035.2 4.246 1.7E−0347.50 Control 7.582 Control 2.879 CT22 5023.1 4.110 2.1E−01 49.46 CT275033.4 4.384 2.4E−02 29. 17 Control 2.750 Control 3.394 CT27 5031.45.380 5.8E−01 10.31 CT75 4873.4 4.829 3.9E−01 9.29 CT27 5033.4 6.9931.0E−01 43.38 CT75 4873.3 6.119 2.2E−03 38.51 Control 4.877 Control4.418 CT27 5035.2 5.220 3.8E−02 89.83 CT76 5044.6 3.723 1.9E−01 9.70Control 2.750 CT76 5041.5 4.025 3.1E−02 18.59 CT6 4943.1 7.564 2.3E−0155.08 CT76 5043.5 3.614 6.2E−01 6.49 CT6 4941.4 7.260 1.8E−01 48.86 CT765041.6 3.651 2.9E−01 7.58 Control 4.877 CT76 5041.9 3.970 5.6E−02 16.98CT75 4873.4 5.384 6.2E−01 10.40 Control 3.394 CT75 4873.3 6.378 1.6E−0130.78 NUE206 6731.2 4.717 1.4E−03 36.19 Control 4.877 NUE206 6732.93.952 1.7E−01 14.11 CT76 5044.6 4.762 2.6E−02 39.62 NUE206 6732.5 3.6247.7E−01 4.64 CT76 5041.5 4.729 6.0E−02 38.66 Control 3.463 CT76 5043.55.470 1.3E−01 60.38 NUE208 8355.3 4.721 7.5E−02 36.30 CT76 5041.9 5.2821.3E−02 54.87 Control 3.463 Control 3.410 NUE209 8192.14 4.584 5.7E−0232.34 NUE206 6731.2 7.467 1.3E−01 95.14 Control 3.463 NUE206 6732.74.706 3.7E−01 22.98 NUE212 8331.1 5.110 2.1E−01 9.85 Control 3.826NUE212 8332.2 5.868 9.1E−02 26.14 NUE206 6731.2 6.249 2.8E−02 92.41NUE212 8331.4 4.910 5.8E−01 5.55 NUE206 6732.5 4.433 4.0E−01 36.49Control 4.652 Control 3.248 NUE221 9801.1 4.34 1.6E−01 13.5 NUE2088354.8 4.799 5.0E−01 17.29 NUE221 9801.7 3.84 9.0E−01 0.4 NUE208 8351.35.763 7.7E−02 40.86 NUE221 9802.8 4.68 2.1E−02 22.5 Control 4.091Control 3.82 NUE209 8192.13 5.110 7.0E−02 24.91 NUE222 8854.1 4.9977.0E−02 7.48 NUE209 8192.14 5.450 1.7E−01 33.21 Control 4.649 Control4.091 NUE223 9613.1 4.236 5.0E−01 10.38 NUE209 8192.14 5.624 5.5E−0273.16 NUE223 9611.5 5.091 6.8E−03 32.67 Control 3.248 NUE223 9612.34.868 1.6E−01 26.86 NUE210 8202.2 5.208 1.0E−01 27.29 Control 3.837Control 4.091 NUE225 9731.7 4.58 4.0E−02 20.0 NUE212 8335.2 6.3382.8E−02 54.92 NUE225 9731.8 4.30 2.0E−01 12.5 NUE212 8334.1 4.5413.4E−01 10.99 NUE225 9732.8 4.09 4.7E−01 6.9 NUE212 8331.4 6.188 1.3E−0151.26 NUE225 9734.5 4.07 4.9E−01 6.5 Control 4.091 NUE225 9734.9 4.262.3E−01 11.5 NUE212 8332.2 8.847 2.5E−01 56.56 Control 3.82 NUE2128331.4 6.998 2.5E−01 23.84 NUE228 10092.2 4.242 1.4E−01 13.89 Control5.651 NUE228 10093.1 4.106 2.5E−01 10.23 NUE221 9801.1 5.06 1.4E−01 29.7Control 3.725 NUE221 9802.8 5.89 1.2E−03 50.9 NUE231 10631.3 4.272.3E−01 11.6 NUE221 9806.1 4.39 5.4E−01 12.3 NUE231 10631.4 4.08 4.8E−016.8 Control NUE231 10633.3 4.34 1.6E−01 13.5 NUE223 9613.1 5.411 2.9E−0116.86 Control 3.82 NUE223 9612.3 5.162 2.9E−01 11.49 NUE233 10174.33.942 4.0E−01 7.65 Control 4.630 NUE233 10174.1 4.973 2.8E−02 35.83NUE223 9611.5 8.701 1.9E−02 67.39 NUE233 10173.5 4.903 2.0E−02 33.89NUE223 9612.3 6.493 2.6E−01 24.90 NUE233 10172.5 4.240 1.3E−01 15.78Control 5.198 NUE233 10173.7 4.289 1.7E−01 17.14 NUE225 9731.7 4.772.7E−01 22.1 Control 3.662 Control 3.90 NUE233 10174.1 4.253 1.5E−0216.86 NUE228 10092.2 5.763 7.2E−02 34.52 NUE233 10173.5 4.101 3.7E−0112.66 NUE228 10093.3 5.099 1.5E−01 19.02 NUE233 10172.5 3.911 1.0E−017.44 NUE228 10093.1 5.468 1.2E−01 27.63 NUE233 10173.7 4.544 1.3E−0124.84 Control 4.284 Control 3.640 NUE231 10631.3 4.31 5.9E−01 10.5NUE234 9162.1 4.574 9.8E−02 23.62 NUE231 10631.4 4.87 2.1E−01 24.8Control 3.700 NUE231 10633.3 6.21 3.6E−03 59.1 NUE235 9693.4 4.9086.4E−02 22.32 Control NUE235 9691.1 4.310 4.9E−01 7.43 NUE233 10174.34.340 2.8E−01 24.27 NUE235 9694.4 4.347 4.0E−01 8.36 NUE233 10174.17.195 2.6E−04 106.04 NUE235 9694.3 5.377 3.4E−02 34.03 NUE233 10173.54.086 3.5E−01 17.00 Control 4.012 NUE233 10173.7 4.955 5.4E−02 41.90NUE239 9192.3 5.241 4.4E−04 36.59 Control 3.492 NUE239 9192.1 4.0415.6E−01 5.31 NUE235 9693.4 6.311 7.2E−03 44.37 NUE239 9191.2 4.0814.2E−01 6.35 NUE235 9691.1 5.246 3.3E−02 20.00 Control 3.837 NUE2359694.4 5.145 1.4E−01 17.69 NUE240 9172.1 4.624 1.3E−02 20.49 NUE2359694.3 6.927 4.8E−02 58.46 Control 3.837 Control 4.371 NUE241 9633.46.137 3.4E−06 52.97 NUE237 9654.4 7.760 1.1E−01 38.68 NUE241 9632.34.772 2.4E−01 18.94 NUE237 9654.1 7.127 3.0E−01 27.37 NUE241 9632.25.157 3.2E−04 28.54 Control 5.596 NUE241 9632.4 5.016 2.0E−01 25.02NUE239 9192.3 8.844 6.5E−05 70.14 Control 4.012 NUE239 9191.2 5.9032.8E−01 13.55 NUE242 9212.1 4.373 4.9E−01 6.96 Control 5.198 NUE2429211.2 4.328 5.2E−01 5.86 NUE240 9172.2 5.902 5.7E−02 27.47 NUE2429213.4 5.474 1.3E−03 33.89 NUE240 9174.3 5.530 1.2E−01 19.43 Control4.088 Control 4.630 NUE242 9212.1 4.552 1.0E−01 29.06 NUE240 9172.17.568 1.8E−02 45.59 Control 3.527 Control 5.198 NUE245 10641.7 4.3889.4E−02 20.56 NUE241 9633.4 9.643 7.2E−07 120.61 NUE245 10641.8 4.6577.4E−03 27.95 NUE241 9632.3 5.344 3.9E−01 22.26 NUE245 10643.4 3.9062.1E−01 7.31 NUE241 9632.2 6.559 3.6E−02 50.05 Control 3.640 NUE2419632.4 6.451 1.3E−01 47.58 NUE246 9033.4 4.695 4.8E−01 7.49 Control4.371 NUE246 9031.1 5.062 8.4E−02 15.90 NUE241 9632.5 5.170 5.1E−0115.54 Control 4.368 NUE241 9632.3 6.198 8.3E−02 38.51 NUE250 9134.14.593 1.6E−01 5.15 NUE241 9632.4 5.754 1.6E−01 28.58 NUE250 9132.2 4.5903.3E−01 5.09 Control 4.475 Control 4.088 NUE242 9212.1 5.873 4.1E−0111.59 NUE251 10181.3 3.907 2.6E−01 7.34 NUE242 9213.4 8.125 1.6E−0254.40 NUE251 10183.2 4.763 7.9E−02 30.87 Control 5.262 Control 3.640NUE242 9212.1 5.679 1.2E−01 67.87 NUE256 10063.4 5.259 1.4E−02 43.63NUE242 9213.4 4.572 2.4E−01 35.15 NUE256 10064.1 4.734 2.3E−02 29.28Control 3.383 NUE256 10061.2 4.281 1.3E−01 16.92 NUE245 10641.8 4.7951.3E−01 22.01 NUE256 10062.4 3.855 7.0E−01 5.28 Control 3.930 NUE25610063.2 5.276 5.5E−03 44.10 NUE246 9033.8 6.003 2.5E−01 20.21 Control3.662 NUE246 9033.4 5.693 4.7E−01 14.00 NUE512 9284.3 4.875 1.0E−0117.48 NUE246 9034.1 6.292 1.7E−01 25.99 NUE512 9282.3 4.442 4.4E−01 7.05NUE246 9031.1 7.329 6.6E−03 46.77 NUE512 9284.4 6.172 3.9E−04 48.73Control 4.994 Control 4.150 NUE250 9134.1 5.762 4.8E−01 9.49 NUE5139681.6 5.009 1.7E−03 30.52 NUE250 9132.2 7.281 2.7E−01 38.35 NUE5139683.2 4.506 8.6E−02 17.42 Control 5.262 Control 3.837 NUE251 10181.34.289 1.9E−01 22.81 NUE514 9404.1 4.333 5.3E−01 5.99 NUE251 10183.24.689 1.4E−01 34.27 NUE514 9404.5 4.906 4.1E−02 20.00 NUE251 10183.14.709 1.9E−01 34.86 NUE514 9403.2 4.451 6.9E−02 8.87 Control 3.492NUE514 9402.5 4.644 2.1E−01 13.59 NUE251 10183.2 6.691 3.8E−02 70.25Control 4.088 NUE251 10181.1 4.687 4.8E−01 19.25 NUE514 9403.2 4.8742.1E−02 38.20 Control 3.930 NUE514 9402.5 4.044 2.9E−01 14.65 NUE25610063.4 7.393 2.9E−02 111.70 Control 3.527 NUE256 10064.1 7.214 2.6E−02106.59 NUE515 9712.5 4.43 1.0E−01 15.9 NUE256 10061.2 6.139 2.2E−0375.81 NUE515 9712.6 4.05 5.0E−01 5.8 NUE256 10062.4 6.337 7.9E−02 81.46NUE515 9713.6 5.34 1.0E−04 39.7 NUE256 10063.2 6.594 1.7E−02 88.81Control 3.82 Control 3.492 NUE520 9771.4 4.327 6.9E−02 16.16 NUE25610061.3 4.798 3.9E−02 22.09 NUE520 9771.7 4.332 1.7E−01 16.28 NUE25610061.2 5.141 1.9E−02 30.82 NUE520 9771.2 4.303 1.2E−01 15.52 NUE25610061.4 5.617 9.8E−02 42.92 NUE520 9771.3 4.345 1.6E−01 16.66 NUE25610063.2 5.303 1.5E−02 34.95 Control 3.725 Control 3.930 NUE520 9771.44.377 1.4E−01 19.54 NUE268 8996.5 7.789 1.6E−02 40.04 NUE520 9771.24.684 3.1E−02 27.93 Control 5.562 NUE520 9771.3 3.878 5.2E−01 5.90NUE512 9284.3 4.930 1.1E−01 21.84 Control 3.662 NUE512 9282.3 5.8731.4E−01 45.13 NUE523 9412.5 4.031 3.9E−01 14.28 NUE512 9284.4 7.9121.6E−03 95.53 NUE523 9414.2 5.032 1.5E−03 42.68 Control 4.047 NUE5239413.4 3.766 2.8E−01 6.78 NUE513 9681.6 6.591 3.6E−02 26.79 Control3.527 Control 5.198 NUE523 9412.5 5.066 6.2E−01 7.98 NUE514 9404.5 6.5709.0E−02 24.84 NUE523 9414.2 5.879 2.1E−04 25.30 Control 5.262 Control4.692 NUE514 9403.2 5.579 1.2E−02 64.94 NUE525 9531.2 5.029 1.0E−0325.34 NUE514 9402.5 4.299 3.2E−01 27.09 NUE525 9534.1 5.116 4.0E−0227.51 Control 3.383 NUE525 9533.1 4.471 2.8E−01 11.43 NUE515 9712.5 4.9326.3 1.9E−01 NUE525 9531.1 5.184 1.9E−01 29.21 NUE515 9712.6 4.09  4.78.1E−01 Control 4.012 NUE515 9713.6 7.39 89.4 1.9E−05 NUE531 10081.55.029 9.2E−02 35.00 Control 3.90 Control 3.725 NUE519 9371.2 7.8684.7E−01 51.36 NUE531 10083.3 4.502 2.7E−03 23.69 NUE519 9371.1 7.8131.7E−01 50.30 NUE531 10081.4 3.894 1.4E−01 6.98 Control 5.198 NUE53110083.2 4.655 3.2E−02 27.89 NUE520 9771.4 4.820 3.0E−01 12.51 NUE53110081.5 5.026 2.1E−02 38.08 NUE520 9771.7 5.879 1.4E−02 37.23 Control3.640 NUE520 9771.2 6.392 3.3E−02 49.20 NUE536 9233.3 5.416 1.2E−0224.00 NUE520 9771.3 7.265 1.4E−02 69.57 Control 4.368 Control 4.284NUE539 10101.5 4.107 4.7E−01 12.17 NUE520 9771.4 6.158 1.8E−02 76.34NUE539 10103.5 4.561 5.0E−02 24.57 NUE520 9771.2 6.839 1.7E−02 95.84NUE539 10101.7 4.953 2.0E−02 35.27 NUE520 9771.3 5.440 5.3E−03 55.77Control 3.662 NUE520 9773.1 4.655 9.8E−02 33.29 NUE539 10101.7 4.3448.5E−02 19.36 Control 3.492 Control 3.640 NUE521 9362.2 4.458 3.1E−0131.79 NUE543 10051.1 4.030 3.5E−01 8.20 NUE521 9363.4 5.071 5.6E−0249.90 NUE543 10052.3 4.347 8.1E−02 16.70 Control 3.383 NUE543 10053.14.034 4.0E−01 8.29 NUE523 9412.5 4.834 3.0E−01 42.92 Control 3.725NUE523 9414.2 5.371 1.7E−04 58.79 NUE563 9452.3 5.668 7.3E−02 41.27Control 3.383 NUE563 9451.2 4.348 2.6E−01 8.38 NUE523 9413.3 6.5325.1E−01 20.78 NUE563 9452.1 4.415 3.9E−01 10.04 NUE523 9414.2 8.4794.6E−02 56.78 Control 4.012 Control 5.408 NUE566 9513.1 4.306 4.5E−017.32 NUE525 9531.2 6.497 1.7E−04 48.62 NUE566 9512.2 4.118 6.3E−01 2.63NUE525 9534.1 6.805 6.9E−02 55.67 NUE566 9512.4 4.411 3.9E−01 9.95NUE525 9531.3 4.928 6.1E−01 12.73 NUE566 9512.1 5.392 9.6E−02 34.39NUE525 9533.1 7.002 3.6E−02 60.17 NUE566 9514.1 5.583 1.8E−05 39.15NUE525 9531.1 8.063 1.5E−01 84.46 Control 4.012 Control 4.371 NUE57410363.4 4.132 3.5E−01 13.52 NUE531 10083.3 4.905 2.4E−02 24.81 NUE57410366.2 4.697 7.2E−02 29.04 NUE531 10081.4 6.308 1.3E−02 60.52 NUE57410366.1 4.264 6.0E−03 17.15 NUE531 10083.2 5.480 8.6E−02 39.45 Control3.640 NUE531 10081.5 7.516 4.3E−02 91.25 NUE581 9724.9 4.35 1.5E−01 13.8Control 3.930 Control 3.82 NUE536 9233.3 7.107 3.3E−02 42.30 NUE5839673.4 5.145 8.6E−02 41.35 Control 4.994 NUE583 9673.2 4.621 1.6E−0226.95 NUE537 9393.3 7.508 5.7E−02 85.53 NUE583 9671.2 4.181 1.0E−0114.88 Control 4.047 NUE583 9671.1 3.903 3.2E−01 7.24 NUE539 10101.55.026 1.1E−01 43.93 Control 3.640 NUE539 10103.5 5.622 7.6E−03 60.99NUE586 9751.1 4.510 4.7E−01 7.36 NUE539 10101.7 6 622 4.1E−03 89.62NUE586 9751.7 5.845 3.0E−03 39.13 Control 3.492 NUE586 9751.3 5.2597.3E−02 25.20 NUE543 10051.1 5.204 9.1E−02 21.47 NUE586 9752.2 4.9031.1E−01 16.71 NUE543 10052.3 4.978 2.0E−01 16.20 NUE586 9752.1 6.6261.3E−05 57.73 NUE543 10051.2 5.086 3.6E−01 18.73 Control 4.201 Control4.284 NUE586 9751.1 5.290 3.0E−01 13.71 NUE544 9764.2 8.303 9.5E−0246.92 NUE586 9751.6 6.090 1.6E−03 30.92 NUE544 9763.3 6.821 1.1E−0120.71 NUE586 9751.3 5.181 3.1E−01 11.38 Control 5.651 NUE586 9752.45.952 2.9E−03 27.96 NUE548 9095.2 7.731 2.2E−01 46.90 NUE586 9752.16.660 2.1E−04 43.17 NUE548 9095.4 7.888 1.3E−01 49.89 Control 4.652NUE548 9091.1 6.011 2.7E−01 14.23 NUE593 10391.2 4.849 8.9E−03 30.18Control 5.262 NUE593 10394.1 4.390 2.4E−01 17.85 NUE554 9115.2 7.6033.2E−02 36.68 NUE593 10394.2 4.698 3.3E−02 26.13 Control 5.562 Control3.725 NUE563 9452.3 9.266 1.7E−01 111.97 NUE592 9741.7 4.08 4.8E−01 6.8NUE563 9451.2 6.068 1.3E−01 38.82 NUE592 9747.4 4.00 6.2E−01 4.8 NUE5639452.1 5.145 1.2E−01 17.70 NUE592 9747.5 4.70 1.8E−02 23.0 Control 4.371Control 3.82 NUE566 9513.1 5.537 2.0E−01 26.67 NUE566 9512.2 5.0861.3E−01 16.36 NUE566 9512.1 7.608 1.0E−01 74.05 NUE566 9514.1 7.7522.2E−03 77.33 Control 4.371 NUE569 9381.2 5.147 2.8E−02 21.78 Control4.226 NUE570 9311.4 4.965 5.6E−01 22.69 NUE570 9314.4 5.327 8.0E−0231.63 NUE570 9314.1 5.093 3.3E−01 25.85 Control 4.047 NUE574 10364.24.318 1.9E−01 9.88 NUE574 10366.2 7.430 5.1E−02 89.06 NUE574 10366.15.260 5.6E−02 33.83 Control 3.930 NUE581 9723.6 4.16 7.4E 6.5 NUE5819724.9 4.93 1.9E 26.3 Control 3.90 NUE583 9673.4 8.986 1.7E−02 128.64NUE583 9673.2 6.359 5.0E−02 61.80 NUE583 9671.2 4.956 1.0E−01 26.11Control 3.930 NUE586 9751.1 5.324 5.1E−01 14.00 NUE586 9751.7 8.9382.6E−02 91.38 NUE586 9751.3 6.250 8.3E−02 33.83 NUE586 9752.2 5.5663.7E−01 19.18 NUE586 9752.1 10.320 9.6E−04 120.99 Control 4.670 NUE5869751.1 7.261 2.8E−01 28.49 NUE586 9751.6 7.902 4.2E−02 39.83 NUE5869751.7 6.250 6.0E−01 10.60 NUE586 9751.3 7.274 9.2E−02 28.71 NUE5869752.4 8.572 6.8E−03 51.70 NUE586 9752.1 9.922 5.6E−02 75.58 Control5.651 NUE587 9643.2 7.007 7.6E−02 50.03 Control 4.670 NUE592 9741.7 4.207.0E 7.7 NUE592 9747.5 5.31 7.3E 36.0 Control 3.90 NUE593 10391.2 5.1672.6E−01 20.60 NUE593 10394.2 6.009 9.4E−02 40.25 Control 4.284

Table 26: Analyses of root performance (root length and coverage) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under limiting nitrogen conditions [low nitrogen or nitrogendeficient conditions (0.75 mM N)] as compared to control plants.“Ave.”=Average; “Incr.”=increment.

The genes presented in Tables 27 and 28, hereinbelow, have improvedplant growth rate when grown under limiting nitrogen growth conditions,compared to control plants. Plants showing fast growth rate confirm abetter plant establishment in soil under nitrogen deficient conditions.Faster growth was observed when growth rate of leaf area as well as rootlength and coverage was measured.

Table 27 and 28 depict analyses of plant growth rate of the leaf area,root coverage and root length when grown under limiting nitrogenconditions [low nitrogen or nitrogen deficient conditions (0.75 mM N)]in plants overexpressing the polynucleotides of some embodiments of theinvention under the regulation of a constitutive promoter (35S).Evaluation of each gene was performed by testing the performance ofseveral events. Some of the genes were evaluated in more than one tissueculture assay and the second experiment confirmed the significantincrement in growth rate. Event with p-value<0.1 was consideredstatistically significant.

TABLE 27 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant growth rate(relative growth rate of leaf area and root coverage) under nitrogendeficient conditions RGR Of Leaf Area RGR Of Roots Coverage Gene % Gene% Name Event # Average p-value Incr. Name Event # Average p-value Incr.CT11 4892.3 0.043 3.1E−01 14.75 CT11 4894.3 1.07 2.6E−01 18.78 CT114893.2 0.054 7.0E−02 43.87 CT11 4892.2 1.02 4.7E−01 12.68 Control 0.038CT11 4892.1 1.17 4.9E−02 29.43 CT11 4894.3 0.071 3.2E−02 37.85 Control0.90 CT11 4892.3 0.077 6.5E−03 47.95 CT22 5023.1 0.49 6.6E−02 51.49 CT114892.2 0.066 8.1E−02 27.89 Control 0.32 Control 0.052 CT27 5033.7 0.515.1E−01 59.31 CT27 5031.4 0.059 2.1E−03 56.19 CT27 5031.4 0.66 4.4E−01106.25 CT27 5035.2 0.050 3.7E−02 31.33 CT27 5035.2 0.48 3.3E−01 50.35Control 0.038 CT27 5033.6 0.46 1.9E−01 41.98 CT27 5035.2 0.052 1.4E−0247.58 CT27 5033.4 0.81 5.0E−02 152.27 Control 0.035 CT27 5033.8 0.364.5E−02 12.91 CT27 5033.4 0.047 1.9E−01 28.65 Control 0.32 CT27 5033.80.062 1.7E−04 66.84 CT6 4943.1 0.93 4.7E−02 60.75 Control 0.037 CT64941.4 0.88 3.3E−02 52.53 CT6 4943.1 0.058 1.9E−02 54.43 Control 0.58CT6 4941.4 0.058 1.2E−02 52.74 CT75 4873.3 0.75 1.1E−01 30.24 Control0.038 Control 0.58 CT76 5044.6 0.059 3.8E−01 13.49 CT76 5041.5 1.295.2E−02 43.20 CT76 5041.5 0.075 2.9E−02 44.40 CT76 5043.5 1.24 5.2E−0237.15 CT76 5043.5 0.082 2.8E−04 58.85 CT76 5041.6 1.01 4.7E−01 11.99CT76 5041.9 0.084 4.2E−03 62.25 Control 0.90 CT76 5041.6 0.086 5.5E−0465.56 CT76 5044.6 0.59 3.6E−02 49.62 Control 0.052 CT76 5043.5 0.671.9E−02 71.45 CT76 5044.6 0.044 2.9E−01 18.54 CT76 5041.6 0.46 4.1E−0117.98 CT76 5041.5 0.050 5.8E−02 36.83 CT76 5041.9 0.64 8.3E−03 64.17CT76 5043.5 0.075 1.6E−07 103.60 Control 0.39 CT76 5041.6 0.053 9.2E−0343.71 NUE206 6731.2 0.88 6.1E−03 107.33 CT76 5041.9 0.055 7.7E−03 48.95NUE206 6732.7 0.54 2.5E−01 28.14 Control 0.037 Control 0.42 CT81 4992.10.074 1.9E−03 43.33 NUE206 6731.2 0.73 3.0E−04 103.93 CT81 4993.5 0.0612.8E−01 17.47 NUE206 6732.9 0.46 1.5E−01 29.09 CT81 4992.2 0.072 2.3E−0238.79 NUE206 6732.5 0.51 1.5E−01 42.93 Control 0.052 Control 0.36 NUE2066731.2 0.035 2.0E−02 39.25 NUE208 8354.8 0.58 3.9E−01 18.43 NUE2066732.7 0.032 6.4E−02 28.96 NUE208 8351.3 0.71 3.7E−02 44.72 Control0.025 Control 0.49 NUE208 8351.3 0.046 1.0E−02 54.30 NUE208 8355.3 0.729.1E−03 100.13 NUE208 8355.3 0.038 9.5E−02 28.29 Control 0.36 Control0.030 NUE209 8192.13 0.63 1.3E−01 28.43 NUE208 8355.3 0.073 2.0E−0255.84 NUE209 8192.14 0.65 1.1E−01 32.34 Control 0.047 Control 0.49NUE209 8192.13 0.043 3.2E−02 44.12 NUE209 8192.14 0.65 4.0E−03 79.86NUE209 8192.14 0.047 6.9E−03 60.54 Control 0.36 Control 0.030 NUE2128332.2 0.64 2.2E−02 70.92 NUE209 8192.13 0.047 2.2E−02 38.99 NUE2128334.1 0.61 1.5E−01 63.17 NUE209 8191.5 0.055 4.8E−04 64.77 Control 0.37NUE209 8192.14 0.041 1.2E−01 22.52 NUE212 8335.2 0.75 1.1E−02 52.25Control 0.033 NUE212 8331.4 0.76 2.4E−02 54.17 NUE209 8192.14 0.0712.5E−03 52.94 Control 0.49 NUE209 8191.3 0.057 2.2E−01 22.44 NUE2128332.2 1.08 2.7E−02 58.34 Control 0.047 NUE212 8331.4 0.87 1.6E−01 27.37NUE212 8335.2 0.041 1.9E−02 40.11 Control 0.68 NUE212 8331.4 0.0468.8E−03 54.77 NUE223 9611.5 1.06 8.9E−04 67.16 Control 0.030 NUE2239612.3 0.77 2.3E−01 21.00 NUE212 8332.1 0.062 4.8E−03 50.03 Control 0.63Control 0.041 NUE228 10092.2 0.70 2.0E−02 41.76 NUE224 9001.3 0.0645.9E−04 41.00 NUE228 10093.3 0.61 1.5E−01 23.30 Control 0.045 NUE22810093.1 0.66 5.5E−02 33.53 NUE230 9154.2 0.054 8.1E−02 19.70 Control0.49 NUE230 9151.2 0.052 1.5E−01 14.88 NUE233 10174.3 0.52 2.2E−01 27.38Control 0.045 NUE233 10174.1 0.86 1.1E−05 111.13 NUE230 9153.3 0.0463.5E−02 23.46 NUE233 10173.7 0.59 4.2E−02 45.30 Control 0.038 Control0.41 NUE233 10174.3 0.047 2.8E−03 52.81 NUE233 10174.1 0.56 8.1E−0222.89 NUE233 10174.1 0.075 5.3E−09 141.80 NUE233 10173.7 0.72 2.7E−0357.48 NUE233 10173.7 0.040 4.3E−02 28.55 Control 0.46 Control 0.031NUE234 9162.1 0.51 5.1E−02 39.40 NUE237 9651.1 0.051 4.5E−01 12.77Control 0.37 NUE237 9654.4 0.059 2.5E−02 31.14 NUE235 9693.4 0.682.5E−03 44.45 NUE237 9654.1 0.056 2.6E−02 24.39 NUE235 9691.1 0.562.0E−01 18.18 Control 0.045 NUE235 9694.4 0.55 2.3E−01 15.87 NUE2399191.1 0.063 1.3E−02 58.77 NUE235 9694.3 0.76 1.1E−03 60.48 Control0.040 Control 0.47 NUE239 9192.3 0.061 5.3E−02 25.48 NUE237 9654.4 0.937.8E=02 39.79 Control 0.048 NUE237 9654.1 0.84 2.4E−01 26.90 NUE2409172.2 0.067 1.5E−03 68.99 Control 0.66 NUE240 9174.3 0.052 1.4E−0129.40 NUE239 9191.1 0.75 6.5E−02 36.48 Control 0.040 Control 0.55 NUE2409172.1 0.068 4.1E−03 41.05 NUE239 9192.3 1.08 7.2E−05 71.00 Control0.048 NUE239 9191.2 0.73 3.5E−01 14.79 NUE241 9633.4 0.053 4.5E−04 58.65Control 0.63 NUE241 9632.2 0.042 7.6E−02 26.55 NUE240 9172.2 0.735.1E−02 33.21 NUE241 9632.4 0.045 6.8E−02 35.24 NUE240 9174.3 0.681.4E−01 23.94 Control 0.033 Control 0.55 NUE241 9632.3 0.056 2.2E−0243.54 NUE240 9172.1 0.93 8.4E−03 46.95 Control 0.039 Control 0.63 NUE2429212.1 0.053 4.2E−03 49.38 NUE241 9633.4 1.05 5.1E−09 121.17 NUE2429213.4 0.041 2.2E−01 15.83 NUE241 9632.3 0.56 3.0E−01 17.76 Control0.036 NUE241 9632.2 0.70 4.2E−03 47.48 NUE245 10641.7 0.064 3.3E−0239.30 NUE241 9632.4 0.68 2.6E−02 42.78 Control 0.046 Control 0.47 NUE2469033.8 0.047 5.0E−01 13.54 NUE241 9632.3 0.73 8.7E−02 37.63 NUE2469033.4 0.053 1.8E−01 27.65 NUE241 9632.4 0.68 1.8E−01 28.48 NUE2469034.1 0.067 1.3E−02 63.07 Control 0.53 Control 0.041 NUE242 9214.1 0.825.3E−01 34.65 NUE248 8981.5 0.059 1.3E−02 42.38 NUE242 9213.4 0.984.4E−04 61.42 Control 0.041 Control 0.41 NUE250 9132.1 0.051 2.9E−0113.50 NUE242 9212.1 0.69 2.3E−03 76.75 NUE250 9132.2 0.051 4.2E−01 12.43NUE242 9213.4 0.55 4.3E−02 40.32 NUE250 9134.1 0.055 5.3E−02 21.91Control 0.39 Control 0.045 NUE245 10641.7 0.67 5.7E−03 46.73 NUE25110181.3 0.052 3.2E−05 67.47 NUE245 10641.8 0.57 4.6E−02 24.96 NUE25110183.2 0.044 1.1E−02 41.23 NUE245 10643.4 0.50 4.1E−01 10.90 NUE25110183.1 0.043 1.1E−02 38.27 Control 0.46 Control 0.031 NUE246 9033.80.72 2.4E−01 22.81 NUE251 10183.2 0.084 2.2E−05 83.75 NUE246 9033.4 0.693.8E−01 17.06 NUE251 10182.1 0.057 8.1E−02 23.66 NUE246 9034.1 0.781.5E−01 32.69 NUE251 10181.1 0.048 7.3E−01 4.28 NUE246 9031.1 0.901.2E−02 52.58 Control 0.046 Control 0.59 NUE256 10063.4 0.045 4.9E−0110.33 NUE248 8981.5 0.70 7.3E−02 30.02 NUE256 10064.1 0.057 1.5E−0237.35 Control 0.53 Control 0.041 NUE250 9134.1 0.68 3.3E−01 12.73 NUE25610063.4 0.061 8.1E−04 96.42 NUE250 9132.2 0.89 3.6E−02 46.62 NUE25610064.1 0.063 1.3E−05 104.87 Control 0.61 NUE256 10061.2 0.065 1.0E−07110.14 NUE251 10183.2 0.81 8.3E−05 77.68 NUE256 10062.4 0.062 2.3E−0599.98 NUE251 10181.1 0.56 1.8E−01 24.08 NUE256 10063.2 0.054 1.7E−0474.87 Control 0.46 Control 0.031 NUE254 8972.4 0.74 6.0E−02 38.45 NUE25610061.2 0.071 6.0E−04 55.03 Control 0.53 NUE256 10061.4 0.068 4.1E−0347.93 NUE256 10063.4 0.88 2.3E−04 115.25 NUE256 10063.2 0.051 3.9E−0111.43 NUE256 10064.1 0.89 1.6E−04 117.15 Control 0.046 NUE256 10061.20.74 5.9E−04 81.36 NUE511 9271.2 0.056 2.6E−02 50.68 NUE256 10062.4 0.774.0E−03 87.01 Control 0.040 NUE256 10063.2 0.78 7.9E−04 90.93 NUE5129282.3 0.072 3.6E−04 68.22 Control 0.41 NUE512 9284.4 0.059 8.0E−0338.67 NUE256 10061.3 0.55 7.7E−02 20.48 Control 0.043 NUE256 10061.20.61 1.1E−02 34.76 NUE514 9404.1 0.047 3.4E−02 30.81 NUE256 10061.4 0.676.4E−03 46.55 NUE514 9402.2 0.041 3.3E−01 14.01 NUE256 10063.2 0.636.6E−03 39.24 NUE514 9403.2 0.042 1.7E−01 17.39 Control 0.46 Control0.036 NUE268 8996.5 0.95 7.5E−03 46.67 NUE516 9291.1 0.051 3.6E−01 12.83Control 0.65 NUE516 9291.4 0.058 5.2E−02 28.67 NUE512 9284.3 0.591.2E−01 24.74 Control 0.045 NUE512 9282.3 0.72 1.5E−02 51.97 NUE5199371.2 0.065 7.6E−02 34.99 NUE512 9284.4 0.94 7.5E−06 98.41 NUE5199371.1 0.059 1.7E−01 22.69 Control 0.47 Control 0.048 NUE513 9681.6 0.771.4E−01 21.72 NUE521 9362.2 0.050 7.7E−03 41.00 Control 0.63 NUE5219361.2 0.041 3.1E−01 15.27 NUE514 9404.1 0.72 2.6E−01 17.95 NUE5219363.4 0.056 1.4E−04 56.51 NUE514 9404.5 0.79 2.9E−02 30.17 Control0.036 Control 0.61 NUE521 9362.2 0.057 3.4E−01 16.01 NUE514 9403.2 0.673.9E−05 71.81 NUE521 9363.4 0.065 6.3E−02 31.54 NUE514 9402.5 0.525.8E−02 34.24 Control 0.049 Control 0.39 NUE523 9412.5 0.048 4.9E−0233.67 NUE519 9371.2 0.97 5.6E−02 52.96 NUE523 9414.2 0.043 9.2E−02 20.21NUE519 9371.1 0.96 2.5E−02 51.45 Control 0.036 Control 0.63 NUE5239412.5 0.058 7.6E−02 41.83 NUE520 9771.4 0.59 2.1E−01 20.79 NUE5239414.2 0.049 2.2E−01 19.88 NUE520 9771.7 0.72 9.4E−03 47.07 NUE5239412.1 0.062 5.2E−03 49.67 NUE520 9771.2 0.78 4.4E−03 59.79 Control0.041 NUE520 9771.3 0.89 5.5E−04 81.39 NUE525 9531.2 0.043 8.9E−02 27.96Control 0.49 NUE525 9534.1 0.042 1.4E−01 27.19 NUE520 9771.4 0.761.5E−03 85.18 NUE525 9531.3 0.046 3.9E−02 36.82 NUE520 9771.2 0.833.7E−04 102.18 NUE525 9533.1 0.048 1.6E−02 42.76 NUE520 9771.3 0.665.4E−03 60.90 NUE525 9531.1 0.045 3.5E−02 36.14 NUE520 9773.1 0.571.1E−01 39.32 Control 0.033 Control 0.41 NUE531 10083.1 0.070 5.6E−0224.91 NUE521 9362.2 0.55 5.4E−02 39.89 NUE531 10082.2 0.067 1.1E−0120.04 NUE521 9361.3 0.46 2.1E−01 18.65 NUE531 10081.4 0.070 1.2E−0124.78 NUE521 9363.4 0.63 1.4E−03 61.36 NUE531 10081.5 0.073 8.2E−0230.25 Control 0.39 Control 0.056 NUE523 9412.5 0.58 5.0E−02 49.67 NUE53110081.4 0.051 4.0E−01 11.96 NUE523 9414.2 0.63 9.9E−06 61.62 NUE53110081.5 0.090 1.3E−05 95.63 Control 0.39 Control 0.046 NUE523 9413.30.80 2.6E−01 24.87 NUE532 9222.4 0.050 1.6E−01 40.00 NUE523 9414.2 1.033.8E−03 60.15 Control 0.036 Control 0.64 NUE535 9082.2 0.040 1.7E−0129.19 NUE523 9412.5 1.03 5.0E−02 44.09 NUE535 9084.2 0.037 2.0E−01 17.89NUE523 9414.2 1.05 7.8E−03 47.15 NUE535 9081.1 0.045 4.7E−02 45.68Control 0.71 NUE535 9083.1 0.059 9.9E−06 91.43 NUE525 9531.2 0.711.1E−03 50.16 NUE535 9084.4 0.046 6.1E−03 49.99 NUE525 9534.1 0.763.0E−03 59.45 Control 0.031 NUE525 9531.3 0.55 3.9E−01 15.44 NUE5379391.2 0.067 8.9E−04 57.56 NUE525 9533.1 0.75 1.1E−03 57.42 NUE5379393.3 0.078 3.4E−06 83.37 NUE525 9531.1 0.88 9.0E−04 85.07 Control0.043 Control 0.47 NUE539 10103.5 0.060 7.5E−02 45.45 NUE527 9201.2 0.916.0E−02 39.82 Control 0.041 Control 0.65 NUE539 10101.5 0.052 6.3E−0468.95 NUE528 9073.1 0.91 8.8E−02 33.70 NUE539 10103.5 0.052 2.7E−0567.92 Control 0.68 NUE539 10101.2 0.058 4.6E−06 85.91 NUE531 10081.40.65 1.2E−01 33.32 NUE539 10101.7 0.067 1.3E−06 115.49 NUE531 10081.50.95 6.1E−03 93.18 Control 0.031 Control 0.49 NUE542 9333.2 0.0581.7E−02 27.22 NUE531 10083.3 0.56 5.7E−02 22.09 Control 0.045 NUE53110081.4 0.76 2.5E−04 67.03 NUE543 10051.2 0.043 4.9E−01 11.08 NUE53110083.2 0.65 7.4E−03 42.46 NUE543 10051.6 0.052 2.6E−02 32.97 NUE53110081.5 0.88 5.5E−05 94.20 Control 0.039 Control 0.46 NUE548 9095.20.058 1.9E−02 45.78 NUE535 9084.2 0.87 1.1E−01 34.81 NUE548 9092.2 0.0541.1E−01 34.83 Control 0.65 Control 0.040 NUE536 9233.3 0.85 2.7E−0245.06 NUE548 9095.2 0.067 2.0E−01 16.93 Control 0.59 NUE548 9095.4 0.0826.7E−03 43.33 NUE537 9393.2 0.50 9.3E−02 28.21 NUE548 9091.1 0.0707.8E−02 21.75 NUE537 9393.3 0.49 8.0E−02 25.71 Control 0.057 Control0.39 NUE554 9115.2 0.067 8.8E−02 26.21 NUE537 9393.3 0.92 5.4E−04 95.13Control 0.053 Control 0.47 NUE560 9424.3 0.069 4.8E−02 39.85 NUE53910101.5 0.62 3.4E−02 50.84 Control 0.049 NUE539 10103.5 0.66 6.2E−0361.79 NUE564 9242.2 0.066 8.5E−03 54.86 NUE539 10101.7 0.80 2.0E−0496.01 Control 0.043 Control 0.41 NUE566 9512.1 0.052 2.2E−02 56.47NUE544 9764.2 1.00 2.7E−02 46.55 Control 0.033 NUE544 9763.3 0.802.7E−01 17.81 NUE567 9263.3 0.053 1.8E−01 25.10 Control 0.68 Control0.043 NUE545 9482.4 0.61 7.1E−02 28.80 NUE568 9471.3 0.051 2.9E−01 14.08Control 0.47 NUE568 9472.2 0.062 5.2E−03 40.02 NUE548 9095.2 0.721.2E−01 30.12 Control 0.045 Control 0.55 NUE570 9314.1 0.064 6.7E−0232.94 NUE548 9095.2 0.96 1.5E−02 57.39 Control 0.048 NUE548 9095.4 0.974.3E−03 59.52 NUE573 9491.4 0.058 6.1E−02 30.87 NUE548 9091.1 0.741.1E−01 22.28 NUE573 9494.3 0.055 1.4E−01 23.73 Control 0.61 Control0.045 NUE550 9141.3 0.83 1.4E−01 28.42 NUE574 10364.2 0.062 1.0E−0234.44 Control 0.65 NUE574 10362.2 0.048 6.7E−01 5.59 NUE554 9115.2 0.931.7E−02 43.12 NUE574 10366.2 0.079 6.5E−05 72.54 Control 0.65 Control0.046 NUE563 9452.3 1.02 3.8E−03 114.90 NUE576 9791.3 0.046 2.5E−0117.12 NUE563 9451.2 0.65 3.0E−02 36.75 NUE576 9792.4 0.050 9.0E−02 27.76Control 0.47 NUE576 9794.1 0.048 9.8E−02 24.04 NUE564 9242.3 0.582.0E−01 21.75 NUE576 9793.3 0.048 1.4E−01 22.67 NUE564 9242.2 0.713.1E−02 49.74 Control 0.039 NUE564 9243.4 0.65 9.3E−02 37.10 NUE5829562.4 0.056 4.1E−02 24.61 Control 0.47 Control 0.045 NUE566 9513.1 0.581.3E−01 23.35 NUE583 9673.1 0.056 3.2E−02 43.43 NUE566 9512.2 0.561.7E−03 17.73 Control 0.039 NUE566 9512.1 0.79 5.1E−03 67.15 NUE5839673.4 0.092 3.5E−05 100.45 NUE566 9514.1 0.86 2.8E−05 80.42 NUE5839673.2 0.063 2.5E−02 38.28 Control 0.47 Control 0.046 NUE567 9263.3 0.667.7E−02 39.99 NUE586 9751.6 0.047 4.2E−01 12.92 Control 0.47 NUE5869751.7 0.049 3.1E−01 19.09 NUE567 9263.3 0.97 5.0E−02 42.53 NUE5869752.4 0.057 2.6E−02 36.93 Control 0.68 NUE586 9752.1 0.058 1.0E−0139.90 NUE569 9381.2 0.60 8.8E−02 20.49 Control 0.041 NUE569 9381.5 0.592.1E−01 19.31 Control 0.50 NUE570 9311.4 0.60 2.9E−01 27.17 NUE5709314.4 0.64 4.5E−02 35.92 NUE570 9314.1 0.61 1.6E−01 29.32 Control 0.47NUE570 9314.4 0.63 2.6E−01 26.50 NUE570 9314.1 0.81 1.1E−02 64.12Control 0.50 NUE571 9304.2 0.84 1.4E−01 30.16 Control 0.64 NUE57410364.2 0.51 2.5E−01 12.29 NUE574 10366.2 0.91 7.6E−05 99.71 NUE57410366.1 0.63 6.2E−03 38.15 Control 0.46 NUE583 9673.4 1.08 7.4E−07136.31 NUE583 9673.2 0.76 3.4E−02 67.32 NUE583 9671.2 0.58 3.4E−02 27.94Control 0.46 NUE586 9751.7 1.08 4.4E−04 90.56 NUE586 9751.3 0.73 1.3E−0128.69 NUE586 9752.1 1.23 1.1E−06 117.25 Control 0.57 NUE586 9751.1 0.861.9E−01 26.09 NUE586 9751.6 0.93 5.1E−02 35.91 NUE586 9751.3 0.899.6E−02 30.27 NUE586 9752.4 1.02 9.5E−03 49.17 NUE586 9752.1 1.166.5E−03 69.78 Control 0.68 NUE587 9643.2 0.85 2.3E−02 50.29 Control 0.57NUE593 10394.2 0.72 1.5E−02 46.89 Control 0.49

Table 27: Analyses of plant growth rate (relative growth rate of leafarea and root coverage) of transgenic plants overexpressing theexogenous polynucleotides of some embodiments of the invention (usingthe cloned or synthetic genes listed in Table 23 above) under theregulation of a constitutive promoter (35S) when grown under limitingnitrogen conditions [low nitrogen or nitrogen deficient conditions (0.75mM N)] as compared to control plants. “Incr.”=increment; “RGR”=relativegrowth rate.

TABLE 28 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant growth rate(relative growth rate of root length) under nitrogen deficientconditions RGR Of Roots Length Gene Name Event # Average p-value % incr.CT1 4841.1 0.325 4.6E−01 14.92 CT1 4844.5 0.386 3.9E−02 36.53 CT1 4841.20.399 5.1E−02 41.25 Control 0.282 CT11 4892.1 0.612 9.8E−02 15.48Control 0.530 CT22 5023.1 0.373 9.6E−02 32.01 Control 0.282 CT27 5033.40.394 5.2E−02 29.10 CT27 5033.8 0.350 2.6E−01 14.96 Control 0.305 CT64945.8 0.460 2.1E−01 17.91 CT6 4943.1 0.548 2.0E−02 40.56 Control 0.390CT75 4873.4 0.473 1.5E−01 21.17 CT75 4873.3 0.532 1.3E−02 36.39 Control0.390 CT76 5044.6 0.408 1.1E−02 33.88 CT76 5043.5 0.389 7.9E−02 27.59CT76 5041.9 0.381 6.4E−02 25.11 Control 0.305 NUE206 6731.2 0.4965.5E−03 49.81 NUE206 6732.7 0.395 1.8E−01 19.18 Control 0.331 NUE2066731.2 0.501 1.2E−05 64.61 NUE206 6732.9 0.417 8.6E−03 37.13 Control0.304 NUE208 8351.3 0.477 7.4E−02 22.59 Control 0.389 NUE208 8355.30.500 7.9E−04 64.40 Control 0.304 NUE209 8192.13 0.506 1.6E−02 30.23NUE209 8192.14 0.475 9.5E−02 22.29 Control 0.389 NUE209 8191.2 0.4215.1E−02 36.60 NUE209 8192.13 0.394 7.1E−02 27.85 NUE209 8191.5 0.4103.0E−02 32.93 Control 0.308 NUE209 8192.14 0.452 5.1E−03 48.66 Control0.304 NUE210 8202.2 0.462 9.4E−02 18.92 Control 0.389 NUE210 6755.30.421 3.2E−02 36.49 Control 0.308 NUE212 8332.2 0.455 1.7E−02 47.71NUE212 8334.1 0.426 1.6E−01 38.40 Control 0.308 NUE212 8331.4 0.5042.3E−02 29.68 Control 0.389 NUE212 8331.1 0.584 6.4E−02 24.96 NUE2128332.2 0.597 8.7E−02 27.76 NUE212 8331.4 0.567 1.3E−01 21.20 Control0.468 NUE223 9611.5 0.537 4.1E−03 35.20 NUE223 9612.3 0.466 2.5E−0117.18 Control 0.397 NUE228 10092.2 0.426 5.6E−02 29.90 NUE228 10093.30.422 8.2E−02 28.77 NUE228 10093.1 0.408 1.1E−01 24.40 Control 0.328NUE233 10174.3 0.391 3.9E−01 14.35 NUE233 10174.1 0.471 4.7E−02 37.60NUE233 10173.5 0.461 5.6E−02 34.78 NUE233 10172.5 0.412 2.3E−01 20.55NUE233 10173.7 0.407 2.8E−01 19.07 Control 0.342 NUE233 10174.1 0.4041.2E−03 29.24 NUE233 10173.5 0.362 2.5E−01 15.71 NUE233 10172.5 0.3626.0E−02 15.79 NUE233 10173.7 0.436 3.6E−03 39.35 Control 0.313 NUE2349162.1 0.426 5.9E−02 25.45 Control 0.340 NUE235 9693.4 0.451 1.4E−0225.62 NUE235 9694.3 0.517 4.3E−04 43.98 Control 0.359 NUE239 9191.10.435 3.5E−01 10.73 NUE239 9194.3 0.482 4.7E−02 22.75 Control 0.393NUE239 9192.3 0.565 2.7E−04 42.18 NUE239 9192.1 0.447 2.6E−01 12.34NUE239 9191.2 0.449 2.2E−01 13.06 Control 0.397 NUE240 9172.2 0.4901.2E−02 24.73 Control 0.393 NUE240 9172.1 0.507 1.4E−02 27.47 Control0.397 NUE241 9633.4 0.554 8.4E−07 54.27 NUE241 9632.3 0.407 2.8E−0113.32 NUE241 9632.2 0.466 1.7E−03 29.87 NUE241 9632.4 0.432 1.5E−0120.52 Control 0.359 NUE242 9212.1 0.429 2.2E−01 13.68 NUE242 9213.40.544 4.7E−05 44.09 Control 0.377 NUE242 9212.1 0.462 1.1E−03 54.42NUE242 9211.2 0.403 7.9E−02 34.60 NUE242 9213.4 0.347 2.6E−01 16.09Control 0.299 NUE245 10643.1 0.351 2.1E−01 12.20 NUE245 10641.7 0.4143.8E−03 32.32 NUE245 10641.8 0.434 2.5E−04 38.90 NUE245 10643.4 0.3772.2E−02 20.56 Control 0.313 NUE246 9033.4 0.504 1.2E−01 21.54 NUE2469034.1 0.510 1.4E−01 22.94 NUE246 9031.1 0.524 5.4E−02 26.35 Control0.414 NUE250 9134.1 0.433 1.4E−01 14.91 NUE250 9132.2 0.482 2.4E−0227.68 Control 0.377 NUE251 10183.1 0.460 6.6E−02 34.46 Control 0.342NUE251 10181.3 0.337 3.8E−01 7.88 NUE251 10183.2 0.485 1.4E−04 55.27NUE251 10182.1 0.391 3.4E−02 25.13 NUE251 10183.1 0.323 6.8E−01 3.24NUE251 10181.1 0.361 2.5E−01 15.54 Control 0.313 NUE252 9011.3 0.4686.8E−03 24.03 NUE252 9012.2 0.438 1.2E−01 16.22 NUE252 9013.2 0.4588.4E−02 21.40 Control 0.377 NUE254 8972.4 0.508 2.7E−03 27.22 Control0.399 NUE256 10063.4 0.507 1.5E−02 48.06 NUE256 10064.1 0.525 4.0E−0353.43 NUE256 10061.2 0.431 1.4E−01 26.08 NUE256 10063.2 0.518 6.8E−0351.45 Control 0.342 NUE256 10061.3 0.383 6.3E−02 22.55 NUE256 10061.20.368 6.1E−02 17.75 NUE256 10061.4 0.432 3.7E−04 38.10 NUE256 10063.20.434 3.8E−05 38.83 Control 0.313 NUE512 9282.3 0.479 5.0E−02 22.43NUE512 9284.4 0.525 3.4E−03 34.40 Control 0.391 NUE513 9681.4 0.4891.7E−01 18.01 NUE513 9683.5 0.518 8.4E−02 24.96 Control 0.414 NUE5139681.6 0.475 6.5E−02 19.62 Control 0.397 NUE513 9683.5 0.515 8.4E−0216.54 Control 0.442 NUE514 9404.1 0.471 2.5E−02 24.98 NUE514 9402.20.445 9.9E−02 17.92 NUE514 9404.5 0.493 4.6E−03 30.81 NUE514 9403.20.443 4.8E−02 17.37 NUE514 9402.5 0.503 3.8E−03 33.24 Control 0.377NUE514 9404.1 0.371 3.0E−02 23.82 NUE514 9403.2 0.471 4.9E−05 57.43NUE514 9402.5 0.442 9.5E−04 47.79 Control 0.299 NUE519 9371.2 0.5131.3E−01 29.00 NUE519 9371.1 0.555 1.8E−02 39.76 Control 0.397 NUE5209771.4 0.486 2.5E−03 48.07 NUE520 9771.7 0.471 1.1E−02 43.62 NUE5209771.2 0.463 9.8E−03 41.14 NUE520 9771.3 0.463 2.4E−02 41.09 Control0.328 NUE520 9771.4 0.476 3.4E−02 39.14 NUE520 9771.2 0.478 3.0E−0239.79 Control 0.342 NUE521 9362.2 0.414 1.2E−02 38.49 NUE521 9361.30.383 8.8E−03 27.99 NUE521 9363.4 0.456 1.0E−04 52.49 Control 0.299NUE523 9412.5 0.410 2.6E−02 36.99 NUE523 9414.2 0.495 1.1E−06 65.32NUE523 9412.1 0.364 7.6E−02 21.51 NUE523 9413.4 0.372 3.3E−02 24.28Control 0.299 NUE523 9412.5 0.525 1.7E−01 18.63 NUE523 9414.2 0.5526.9E−03 24.85 Control 0.442 NUE525 9531.2 0.465 3.5E−03 29.55 NUE5259534.1 0.506 5.7E−04 40.93 NUE525 9531.1 0.494 4.1E−03 37.67 Control0.359 NUE531 10082.2 0.413 1.1E−01 25.96 NUE531 10081.5 0.451 5.6E−0237.34 Control 0.328 NUE531 10083.3 0.387 8.0E−03 23.90 NUE531 10082.20.359 2.2E−01 14.68 NUE531 10081.4 0.366 5.7E−02 17.00 NUE531 10083.20.445 5.0E−04 42.20 NUE531 10081.5 0.478 2.9E−05 52.87 Control 0.313NUE536 9233.3 0.511 8.2E−02 23.25 Control 0.414 NUE537 9393.2 0.4092.5E−03 36.75 NUE537 9393.3 0.415 1.8E−03 38.64 Control 0.299 NUE5379393.3 0.496 1.4E−02 26.83 Control 0.391 NUE539 10101.5 0.454 9.5E−0232.65 NUE539 10103.5 0.436 1.2E−01 27.43 NUE539 10101.7 0.527 4.4E−0353.99 Control 0.342 NUE539 10101.7 0.420 2.4E−03 34.28 Control 0.313NUE544 9764.2 0.581 9.7E−02 24.20 Control 0.468 NUE548 9095.2 0.4953.1E−02 31.12 NUE548 9095.4 0.541 1.3E−03 43.44 NUE548 9091.1 0.4368.2E−02 15.49 Control 0.377 NUE550 9141.3 0.469 9.2E−02 27.80 Control0.367 NUE563 9452.3 0.513 4.5E−03 42.86 Control 0.359 NUE566 9512.10.448 7.9E−02 24.77 NUE566 9514.1 0.530 1.3E−05 47.65 Control 0.359NUE570 9314.4 0.477 6.1E−02 22.12 NUE570 9314.1 0.436 3.3E−01 11.56Control 0.391 NUE570 9314.1 0.522 5.4E−02 26.80 Control 0.411 NUE57410363.4 0.384 9.0E−02 22.79 NUE574 10364.2 0.369 3.4E−02 18.18 NUE57410362.2 0.372 4.0E−02 19.10 NUE574 10366.2 0.505 1.3E−05 61.62 NUE57410366.1 0.403 1.8E−03 28.75 Control 0.313 NUE583 9673.1 0.337 3.3E−017.83 NUE583 9673.4 0.511 4.9E−04 63.57 NUE583 9673.2 0.445 1.3E−04 42.20NUE583 9671.2 0.373 5.7E−02 19.44 NUE583 9671.1 0.356 1.3E−01 13.96Control 0.313 NUE586 9751.1 0.466 3.8E−01 12.01 NUE586 9751.7 0.5611.4E−02 34.87 NUE586 9752.1 0.616 6.6E−04 48.10 Control 0.416 NUE5869751.6 0.578 9.9E−02 23.61 NUE586 9751.3 0.544 2.5E−01 16.32 NUE5869752.4 0.585 6.0E−02 25.16 NUE586 9752.1 0.611 3.8E−02 30.58 Control0.468 NUE593 10394.2 0.446 2.9E−02 35.91 Control 0.328

Table 28: Analyses of plant growth rate (relative growth rate of rootlength) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under limiting nitrogenconditions [low nitrogen or nitrogen deficient conditions (0.75 mM N)]as compared to control plants. “Incr.”=increment; “RGR”=relative growthrate.

The genes presented in Tables 29 and 30, hereinbelow, have improvedplant NUE since they produced larger plant biomass when grown understandard nitrogen growth conditions, compared to control plants,indicating the high ability of the plant to better metabolize thenitrogen present in the medium.

Tables 29 and 30 depict analyses of plant biomass (plant fresh and dryweight and leaf area) when grown under standard nitrogen conditions[normal or regular growth conditions (15 mM N)] in plants overexpressingthe polynucleotides of some embodiments of the invention under theregulation of a constitutive promoter (35S). Evaluation of each gene wasperformed by testing the performance of several events. Some of thegenes were evaluated in more than one tissue culture assay and theresults obtained were repeated. Event with p-value<0.1 was consideredstatistically significant.

TABLE 29 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (freshand dry weight) under standard nitrogen conditions Plant Fresh Weight[mg] Plant Dry Weight [mg] Gene % Gene p- % Name Event # Average p-valueincr. Name Event # Average value incr. CT1 4841.1 224.68 5.7E−03 44.54CT11 4894.3 10.93 1.0E−01 57.88 CT1 4844.3 220.28 1.3E−01 41.71 CT114892.2 11.00 2.3E−02 58.84 Control 155.44 CT11 4892.3 9.35 1.6E−01 35.02CT11 4892.2 327.13 1.2E−02 41.78 CT11 4893.2 7.20 7.0E−01 3.97 CT114892.3 321.38 4.4E−02 39.29 CT11 4892.1 12.40 1.0E−01 79.06 Control230.73 Control 6.93 CT11 4893.2 293.83 1.8E−02 70.30 CT11 4894.2 6.705.8E−01 8.06 Control 172.54 CT11 4893.2 12.73 5.8E−03 105.24 CT22 5023.1249.48 1.3E−02 60.50 Control 6.20 Control 155.44 CT27 5033.6 7.401.9E−01 79.39 CT27 5033.6 234.13 1.1E−01 148.18 CT27 5033.8 7.50 9.2E−0281.82 CT27 5033.8 192.50 8.4E−03 104.05 CT27 5033.5 5.55 1.9E−01 34.55CT27 5033.5 143.73 3.1E−01 52.35 Control 4.13 Control 94.34 CT27 5033.78.23 1.5E−01 32.66 CT27 5033.7 224.58 1.2E−01 30.16 CT27 5035.2 13.102.1E−04 111.29 CT27 5035.2 343.65 2.2E−02 99.17 CT27 5031.4 9.28 8.2E−0349.60 CT27 5031.4 255.88 3.0E−03 48.30 CT27 5033.6 8.15 2.7E−01 31.45Control 172.54 CT27 5033.4 7.95 4.7E−02 28.23 CT76 5041.7 292.55 5.6R-0226.80 CT27 5033.8 8.90 2.5E−02 43.55 CT76 5043.5 415.05 1.4E−03 79.89CT27 5033.5 7.63 2.1E−01 22.98 Control 230.73 Control 6.20 CT76 5044.6239.08 2.1E−03 153.43 CT6 4943.1 7.83 3.2E−01 26.21 CT76 5041.5 209.101.6E−03 121.65 CT6 4945.9 7.63 1.8E−01 22.98 CT76 5043.5 272.60 2.7E−02188.96 CT6 4941.4 9.28 2.9E−02 49.60 CT76 5041.6 124.75 3.9E−02 32.24Control 6.20 CT76 5041.9 245.20 7.1E−02 159.92 CT75 4874.4 9.35 1.2E−0250.81 Control 94.34 Control 6.20 CT81 4992.1 381.73 3.3E−04 65.45 CT765044.6 9.40 1.6E−01 35.74 CT81 4992.2 305.85 2.8E−01 32.56 CT76 5043.517.23 7.8E−06 148.74 Control 230.73 CT76 5041.6 10.03 9.7E−02 44.89NUE209 8192.14 217.23 3.8E−02 86.30 Control 6.93 Control 116.60 CT765044.6 7.43 5.8E−02 80.00 NUE210 8202.1 279.53 1.1E−01 139.73 CT765041.5 9.70 1.0E−04 135.15 NUE210 8201.3 250.90 4.8E−02 115.18 CT765041.7 5.03 4.5E−01 21.82 Control 116.60 CT76 5043.5 10.88 1.3E−02163.64 NUE211 8263.5 162.35 5.0E−02 31.43 CT76 5041.9 8.95 1.1E−02116.97 Control 123.53 Control 4.13 NUE212 8332.1 253.75 1.0E−01 105.42CT81 4992.1 11.20 4.1E−02 61.73 NUE212 8335.2 169.28 4.9E−02 37.03 CT814993.5 8.60 3.6E−01 24.19 Control 123.53 CT81 4992.2 8.63 3.4E−01 24.55NUE212 8335.2 221.83 2.0E−02 90.24 CT81 4995.5 7.90 3.8E−01 14.08 NUE2128331.4 163.88 2.7E−01 40.54 Control 6.93 Control 116.60 NUE206 6732.913.68 7.9E−03 43.38 NUE212 8332.1 116.43 1.8E−01 29.34 NUE206 6731.214.13 4.3E−01 48.19 NUE212 8334.1 128.33 8.1E−02 42.56 NUE206 6732.512.98 1.8E−02 36.04 NUE212 8331.4 143.63 3.0E−02 59.56 NUE206 6732.210.98 3.6E−01 15.07 Control 90.01 Control 9.54 NUE221 9802.8 149.353.9E−03 58.7 NUE208 8354.8 8.20 2.7E−02 78.75 NUE221 9806.1 209.181.7E−08 122.3 NUE208 8355.3 5.78 3.3E−01 25.89 Control 94.09 Control4.59 NUE222 8851.3 240.70 6.1E−02 106.43 NUE208 8354.8 6.15 3.1E−0214.42 NUE222 8852.4 138.15 3.1E−01 18.48 Control 5.38 Control 116.60NUE208 8354.8 16.45 1.9E−03 72.48 NUE224 9002.4 279.08 6.1E−02 32.66NUE208 8354.5 15.58 2.4E−03 63.30 Control 210.36 NUE208 8355.3 12.409.6E−02 30.01 NUE224 9002.4 159.13 2.6E−01 14.58 Control 9.54 NUE2249002.2 268.95 3.8E−03 93.66 NUE209 8192.1 7.73 1.5E−01 68.39 NUE2249001.3 181.65 3.2E−02 30.80 NUE209 8191.5 7.13 7.1E−02 55.31 Control138.88 Control 4.59 NUE225 9732.8 117.00 2.2E−01 24.4 NUE209 8191.510.83 6.2E−01 162.42 Control 94.09 NUE209 8191.3 15.40 6.3E−03 NUE2279853.1 197.68 9.4E−02 55.51 Control 9.54 Control 127.11 NUE210 8202.110.95 1.0E−02 138.69 NUE229 8862.2 75.00 1.2E−02 26.32 NUE210 8201.38.98 5.5E−02 95.64 NUE229 8862.5 74.03 1.3E−01 24.67 Control 4.59 NUE2298864.2 84.93 3.9E−02 43.03 NUE210 8202.1 4.28 2.4E−02 41.91 Control59.38 NUE210 8751.4 4.33 2.4E−02 43.57 NUE230 9154.2 171.38 4.4E−0123.40 NUE210 6755.3 3.85 2.4E−01 27.80 NUE230 9151.2 203.78 3.1E−0246.73 NUE210 8201.2 3.93 2.1E−01 30.29 Control 138.88 Control 3.01NUE231 10633.3 199.70 1.6E−07 112.2 NUE211 8265.1 7.38 8.8E−02 60.76Control 94.09 Control 4.59 NUE233 10174.3 139.08 9.0E−02 44.46 NUE2128335.2 9.53 7.3E−02 107.63 NUE233 10174.1 190.05 5.5E−04 97.40 Control4.59 NUE233 10173.7 143.98 3.5E−03 49.55 NUE212 8334.1 4.20 7.4E−0239.42 Control 96.28 NUE212 8331.4 5.08 5.7E−02 68.46 NUE235 9694.2171.15 1.0E−01 23.24 Control 3.01 NUE235 9691.1 172.20 7.8E−02 24.00NUE221 9802.8. 7.50 2.5E−03 56.3 NUE235 9693.3 194.48 5.4E−02 40.04NUE221 9806.1. 9.08 3.5E−06 89.1 Control 138.88 Control 4.80 NUE2379651.1 293.05 3.8E−02 111.02 NUE222 8851.3 11.60 4.2E−02 152.86 NUE2379652.3 167.10 1.1E−01 20.32 Control 4.59 NUE237 9654.4 195.80 1.4E−0140.99 NUE224 9002.2 10.13 5.4E−02 83.67 Control 138.88 NUE224 9001.37.03 1.1E−01 27.44 NUE237 9651.1 191.70 1.8E−02 26.32 Control 5.51Control 151.76 NUE227 9851.2 5.88 1.4E−01 24.34 NUE239 9192.1 245.533.6E−02 56.82 NUE227 9853.1 8.88 4.0E−02 87.83 Control 156.56 Control4.73 NUE240 9172.1 212.68 7.3E−02 35.84 NUE228 10092 7.90 2.0E−01 45.29NUE240 9174.3 255.50 2.6E−01 NUE228 10093 7.98 8.6E−02 46.67 Control156.56 NUE228 10093 6.68 5.3E−03 22.76 NUE241 9631.3 166.03 6.6E−0230.61 Control 5.44 NUE241 9632.5 185.58 1.4E−02 45.99 NUE229 8862.2 3.904.1E−02 30.54 NUE241 9632.4 219.43 8.4E−03 72.62 NUE229 8862.5 3.802.7E−01 27.20 Control 127.11 NUE229 8864.2 4.45 2.9E−02 48.95 NUE2429212.1 140.78 3.9E−02 59.41 Control 2.99 NUE242 9214.1 129.18 1.5E−0146.27 NUE230 9154.2 7.38 1.0E−01 33.79 NUE242 9213.2 101.43 3.7E−0114.85 NUE230 9151.2 7.48 5.8E−02 35.60 NUE242 9213.4 146.30 3.0E−0265.66 Control 5.51 Control 88.31 NUE231 10632.2 5.53 4.1E−01 15.1 NUE2449061.1 164.20 8.9E−04 45.23 NUE231 10633.3 11.43 2.0E−11 138.0 NUE2449061.5 143.40 4.8E−01 26.83 Control 4.80 Control 113.06 NUE233 101746.13 1.2E−02 58.58 NUE246 9033.6 273.05 8.9E−03 43.57 NUE233 10174 8.632.1E−04 123.30 NUE246 9033.4 241.48 4.8E−01 26.97 NUE233 10174 5.108.2E−02 32.04 NUE246 9034.1 224.08 2.5E−01 17.82 Control 3.86 NUE2469031.1 232.65 3.3E−01 22.33 NUE234 9163.5 4.28 8.7E−02 43.10 Control190.19 NUE234 9162.1 4.60 1.3E−01 53.97 NUE246 9034.1 160.45 1.8E−0241.91 Control 2.99 Control 113.06 NUE235 9694.2 7.35 2.2E−01 33.33NUE246 9033.4 185.78 4.3E−01 16.45 NUE235 9691.1 7.90 1.4E−01 43.31NUE246 9033.8 205.95 1.8E−01 29.09 NUE235 9693.3 6.98 6.2E−02 26.53NUE246 9034.1 228.95 2.4E−03 43.51 Control 5.51 Control 159.54 NUE2379651.1 10.20 2.9E−02 85.03 NUE248 8982.4 275.80 2.2E−02 45.01 NUE2379652.3 6.68 6.3E−02 21.09 NUE248 8981.5 343.28 1.1E−02 80.49 NUE2379654.4 8.25 1.1E−02 49.66 NUE248 8984.1 294.45 1.5E−01 54.82 Control5.51 NUE248 8981.2 245.25 1.1E−01 28.95 NUE237 9651.1 6.98 4.0E−02 26.53Control 190.19 Control 5.51 NUE248 8982.4 118.75 1.6E−01 37.56 NUE2399191.2 8.80 7.6E−02 19.32 NUE248 8984.1 124.38 2.5E−02 44.08 Control7.38 NUE248 8981.5 140.05 4.8E−02 62.24 NUE241 9631.3 6.43 8.5E−02 35.98NUE248 8983.1 114.05 3.2E−01 32.12 NUE241 9632.5 8.33 2.8E−04 76.19Control 86.33 NUE241 9632.3 6.55 1.6E−02 38.62 NUE249 9122.5 145.734.6E−02 68.82 NUE241 9632.4 8.03 1.0E−04 70.02 NUE249 9121.4 112.833.6E−01 30.71 Control 4.73 NUE249 9123.3 107.98 2.1E−01 25.08 NUE2449061.1 5.65 6.3E−02 34.52 Control 86.33 NUE244 9061.5 5.88 6.7E−02 39.88NUE250 9133.2 182.70 4.3E−02 31.56 Control 4.20 NUE250 9134.1 216.852.5E−02 56.15 NUE246 9033.6 8.98 2.9E−02 28.90 Control 138.88 NUE2469033.4 8.28 3.1E−01 18.85 NUE251 10181.3 143.00 4.0E−02 48.53 Control6.96 NUE251 10183.2 146.38 2.4E−02 52.04 NUE246 9034.1 5.60 7.3E−0233.33 NUE251 10183.1 128.05 3.6E−01 33.00 Control 4.20 Control 96.28NUE246 9033.4 6.28 1.7E−01 18.40 NUE254 8972.2 173.28 8.8E−02 100.72NUE246 9033.8 8.63 5.2E−04 62.74 NUE254 8974.1 130.38 4.1E−02 51.03NUE246 9034.1 8.35 2.5E−04 57.55 Control 86.33 Control 5.30 NUE25610063.4 132.65 1.4E−02 37.78 NUE248 8982.4 9.88 2.2E−02 41.83 NUE25610064.1 212.63 1.5E−04 120.85 NUE248 8981.5 11.78 1.3E−01 69.12 NUE25610061.2 151.98 1.2E−01 57.86 NUE248 8984.1 10.25 1.4E−01 47.22 NUE25610062.4 152.75 1.5E−01 58.66 NUE248 8981.2 7.55 6.5E−01 8.44 NUE25610063.2 162.50 2.1E−01 68.79 Control 6.96 Control 96.28 NUE248 8984.17.15 1.2E−01 43.00 NUE267 8962.1 185.23 1.6E−02 63.83 NUE248 8981.5 8.651.9E−02 73.00 Control 113.06 Control 5.00 NUE268 8994.5 228.80 8.7E−0264.46 NUE250 9134.3 8.48 1.3E−02 49.67 NUE268 8992.1 204.08 2.2E−0146.69 Control 5.66 NUE268 8996.5 146.34 7.6E−02 5.19 NUE250 9132.1 11.181.5E−01 102.72 Control 139.13 NUE250 9133.2 7.88 2.6E−02 42.86 NUE2699101.1 95.83 1.4E−02 79.28 NUE250 9132.2 8.55 3.2E−02 55.10 NUE2699102.2 89.05 7.7E−05 66.60 NUE250 9134.1 8.88 4.5E−02 61.00 NUE2699102.3 117.90 6.5E−02 120.58 Control 5.51 NUE269 9103.1 83.60 7.0E−0256.41 NUE250 9134.1 3.53 1.8E−02 17.99 NUE269 9103.3 82.45 1.6E−02 54.26NUE250 9131.2 4.38 7.2E−02 46.44 Control 53.45 Control 2.99 NUE5129284.2 94.55 9.0E−02 20.60 NUE251 10181 5.98 4.9E−02 54.69 NUE512 9284.392.98 4.2E−01 18.59 NUE251 10183 6.63 3.6E−03 71.52 NUE512 9283.1 91.308.6E−02 16.45 Control 3.86 NUE512 9282.3 92.85 5.7E−02 18.43 NUE2548972.2 6.43 2.1E−02 52.98 NUE512 9281.3 105.50 2.1E−01 34.57 Control4.20 Control 78.40 NUE254 8972.2 9.28 3.3E−02 85.50 NUE514 9404.1 158.733.8E−02 79.73 Control 5.00 Control 88.31 NUE256 10063 6.10 1.7E−03 57.93NUE515 9712.5 104.98 5.6E−01 11.6 NUE256 10064 9.55 2.9E−07 147.25NUE515 9713.6 185.55 4.0E−06 97.2 NUE256 10061 6.30 5.2E−02 63.11Control 94.09 NUE256 10062 7.65 1.1E−01 98.06 NUE516 9291.1 230.008.3E−02 65.62 NUE256 10063 6.33 4.9E−03 63.75 NUE516 9291.4 227.135.7E−02 63.55 Control 3.86 Control 138.88 NUE267 8962.1 6.43 5.0E−0352.98 NUE520 9771.4 137.73 5.8E−02 43.05 Control 4.20 NUE520 9771.7160.25 3.5E−03 66.45 NUE268 8994.5 7.18 8.4E−02 59.44 NUE520 9771.2158.98 1.3E−02 65.13 NUE268 8996.3 6.85 1.9E−02 52.22 NUE520 9771.3148.40 6.5E−02 54.14 NUE268 8996.5 7.00 2.8E−03 55.56 Control 96.28Control 4.50 NUE521 9361.2 167.53 7.3E−05 89.70 NUE512 9284.2 4.201.6E−02 46.72 NUE521 9363.4 180.95 7.6E−03 104.90 NUE512 9284.3 3.581.7E−01 24.89 Control 88.31 NUE512 9283.1 4.35 1.8E−02 51.97 NUE5239412.1 271.35 1.0E−01 42.67 NUE512 9282.3 4.18 2.6E−02 45.85 Control190.19 NUE512 9281.3 4.93 1.9E−02 72.05 NUE523 9413.3 184.25 7.3E−0228.51 Control 2.86 NUE523 9413.4 180.55 2.2E−01 25.93 NUE512 9284.2 6.002.3E−03 53.35 Control 143.37 Control 3.91 NUE527 9202.6 152.18 6.1E−019.38 NUE514 9404.1 7.90 6.3E−02 61.64 NUE527 9203.2 249.95 7.5E−02 79.66Control 4.89 NUE527 9201.2 273.53 4.2E−04 96.60 NUE515 9713.6 8.381.0E−04 74.5 Control 139.13 Control 4.80 NUE527 9204.2 101.70 1.3E−0290.27 NUE519 9371.1 12.15 1.4E−01 64.75 NUE527 9202.6 82.40 3.4E−0254.16 NUE519 9371.2 14.15 3.5E−01 91.86 NUE527 9201.1 120.30 3.6E−03125.07 NUE519 9373.1 9.20 2.4E−01 24.75 NUE527 9203.2 84.63 2.6E−0358.33 Control 7.38 NUE527 9204.1 68.55 1.2E−01 28.25 NUE520 9771.4 5.731.5E−01 48.22 Control 53.45 NUE520 9771.7 6.60 5.4E−02 70.87 NUE5329222.4 210.65 3.5E−01 51.41 NUE520 9771.2 8.05 7.6E−03 108.41 NUE5329222.1 168.45 8.4E−02 21.08 NUE520 9771.3 5.73 2.9E−02 48.22 NUE5329223.5 210.15 7.6E−02 51.05 Control 3.86 Control 139.13 NUE523 9412.19.03 6.0E−02 29.62 NUE535 9081.1 117.15 3.0E−01 21.68 Control 6.96NUE535 9083.1 235.35 7.7E−02 144.46 NUE527 9201.2 8.78 7.7E−02 95.00NUE535 9084.4 128.88 5.4E−02 33.86 Control 4.50 NUE535 9082.1 114.833.1E−01 19.27 NUE531 10083 7.05 1.2E−01 29.66 Control 96.28 NUE531 100828.90 7.5E−02 63.68 NUE535 9082.2 85.55 5.9E−03 60.06 NUE531 10081 8.602.4E−01 58.16 NUE535 9086.2 120.63 1.3E−02 125.68 NUE531 10082 9.431.6E−02 73.33 NUE535 9086.3 86.67 1.2E−01 62.15 Control 5.44 NUE5359081.1 90.65 4.3E−03 69.60 NUE531 10081 8.48 4.2E−02 32.13 NUE535 9084.469.83 2.2E−02 30.64 NUE531 10082 8.95 1.2E−01 39.53 Control 53.45Control 6.41 NUE537 9393.3 207.43 6.7E−02 30.28 NUE532 9222.4 8.281.4E−01 83.89 Control 159.21 NUE532 9222.1 6.53 8.4E−02 45.00 NUE5389782.1 203.68 4.0E−02 60.23 NUE532 9223.3 6.08 7.5E−02 35.00 Control127.11 NUE532 9223.5 6.70 1.8E−01 48.89 NUE539 10101.5 146.60 3.7E−0352.27 Control 4.50 NUE539 10103.5 126.33 7.8E−02 31.21 NUE535 9083.110.90 5.6E−02 182.20 NUE539 10101.2 190.80 5.0E−03 98.18 Control 3.86NUE539 10101.7 173.78 2.0E−04 80.50 NUE537 9391.1 6.48 9.9E−02 65.50Control 96.28 NUE537 9393.3 5.53 2.9E−01 41.21 NUE542 9332.1 196.483.3E−02 41.48 Control 3.91 Control 138.88 NUE538 9782.1 8.30 2.8E−0575.66 NUE544 9763.3 169.78 8.8E−02 26.31 Control 4.73 Control 134.41NUE539 10102 6.83 1.4E−03 76.70 NUE549 9343.6 200.95 9.7E−02 32.41NUE539 10101 9.15 2.0E−02 136.89 NUE549 9343.7 205.95 2.8E−01 35.71NUE539 10102 7.80 1.6E−02 101.94 Control 151.76 Control 3.86 NUE5509144.4 128.13 5.6E−03 139.71 NUE543 10052 5.90 7.1E−02 24.87 NUE5509141.3 116.60 5.0E−07 118.15 Control 4.73 NUE550 9143.1 124.23 1.9E−02132.41 NUE544 9764.2 8.25 1.1E−01 53.49 NUE550 9143.4 98.70 5.2E−0284.66 NUE544 9763.3 8.25 2.7E−02 53.49 Control 53.45 Control 5.38 NUE5509143.1 197.68 2.6E−01 42.08 NUE548 9095.2 7.50 7.6E−02 32.45 NUE5509143.4 174.85 1.4E−01 25.68 NUE548 9095.4 8.18 1.7E−01 44.37 NUE5509142.2 240.83 7.2E−05 73.10 NUE548 9091.1 7.68 2.1E−01 35.54 Control139.13 Control 5.66 NUE553 9181.5 76.85 1.9E−03 43.78 NUE548 9095.210.17 2.7E−02 71.23 NUE553 9185.2 74.85 2.4E−01 40.04 NUE548 9092.2 8.158.9E−02 37.26 NUE553 9184.3 61.65 5.6E−01 15.34 Control 5.94 NUE5539182.2 72.28 1.3E−01 35.22 NUE549 9343.7 7.25 6.9E−02 31.52 Control53.45 Control 5.51 NUE554 9111.4 135.30 6.7E−02 153.13 NUE550 9141.35.80 3.0E−01 28.89 Control 53.45 NUE550 9143.4 5.73 8.3E−03 27.22 NUE5639453.2 270.58 1.2E−01 53.26 NUE550 9142.2 8.08 1.6E−02 79.44 NUE5639452.3 207.35 4.2E−01 17.45 Control 4.50 NUE563 9451.2 273.50 4.7E−0254.91 NUE554 9115.2 6.40 4.5E−02 42.22 Control 176.55 Control 4.50NUE564 9242.3 113.35 4.7E−02 44.58 NUE560 9424.3 8.85 1.7E−03 65.64NUE564 9242.4 90.95 8.5E−02 16.01 NUE560 9422.1 6.88 4.7E−02 28.68NUE564 9244.1 94.08 1.3E−02 19.99 Control 5.34 Control 78.40 NUE5629252.8 8.43 3.0E−02 57.69 NUE566 9512.4 257.28 1.4E−02 45.72 Control5.34 Control 176.55 NUE567 9261.3 4.10 6.3E−02 43.23 NUE567 9263.2130.00 7.3E−03 65.82 Control 2.86 NUE567 9261.3 93.50 8.4E−02 19.26NUE568 9471.3 7.63 1.8E−02 38.32 NUE567 9261.4 112.75 2.2E−02 43.81Control 5.51 NUE567 9263.3 84.55 5.9E−01 7.84 NUE569 9381.2 4.40 1.7E−0253.71 Control 78.40 NUE569 9381.5 4.90 9.0E−02 71.18 NUE568 9471.3230.43 4.1E−02 51.83 NUE569 9381.3 4.73 2.4E−03 65.07 NUE568 9461.2186.87 2.5E−01 23.13 Control 2.86 NUE568 9474.4 187.77 2.0E−01 23.72NUE570 9311.4 3.63 1.4E−01 26.64 NUE568 9472.2 195.70 3.0E−01 28.95NUE570 9313.3 4.33 4.3E−02 51.09 NUE568 9462.3 172.65 5.1E−01 13.76NUE570 9314.4 4.78 8.5E−03 66.81 Control 151.76 NUE570 9314.1 4.333.0E−02 51.09 NUE569 9384.4 90.90 2.1E−01 15.94 NUE570 9312.3 5.234.2E−04 82.53 NUE569 9381.2 124.28 7.9E−03 58.51 Control 2.86 NUE5699381.5 130.40 4.3E−02 66.33 NUE571 9304.2 8.98 5.6E−02 67.98 NUE5699381.3 99.18 1.7E−01 26.50 NUE571 9303.2 8.63 2.3E−03 61.43 NUE5699384.2 99.08 9.4E−02 26.37 NUE571 9301.4 7.13 6.3E−02 33.36 Control78.40 Control 5.34 NUE570 9313.3 110.70 1.2E−01 41.20 NUE571 9304.3 6.502.4E−04 127.07 NUE570 9314.4 119.08 1.2E−02 51.88 NUE571 9304.2 6.052.8E−02 111.35 NUE570 9314.1 109.93 8.7E−03 40.21 NUE571 9303.2 4.984.2E−03 73.80 NUE570 9312.3 149.30 8.0E−03 90.43 NUE571 9301.4 4.134.1E−02 44.10 Control 78.40 NUE571 9302.3 4.03 3.4E−02 40.61 NUE5719304.2 212.53 1.1E−01 48.23 Control 2.86 NUE571 9303.2 240.93 3.8E−0268.04 NUE572 9321.3 4.95 9.0E−02 72.93 NUE571 9302.1 177.58 4.0E−0123.86 NUE572 9324.3 4.55 2.4E−02 58.95 NUE571 9301.4 209.80 1.0E−0146.33 NUE572 9321.1 4.80 4.7E−03 67.69 NUE571 9302.3 199.13 2.6E−0138.89 NUE572 9322.2 4.35 1.5E−02 51.97 Control 143.37 Control 2.86NUE571 9304.3 124.43 1.2E−02 58.71 NUE573 9491.4 7.28 1.8E−03 31.97NUE571 9304.2 123.90 4.5E−02 58.04 Control 5.51 NUE571 9303.2 106.001.7E−02 35.20 NUE576 9793.3 8.03 7.1E−04 69.84 Control 78.40 Control4.73 NUE572 9322.1 124.90 3.5E−02 59.31 NUE581 9723.6 6.28 9.3E−02 30.7NUE572 9324.3 115.85 2.7E−03 47.77 NUE581 9724.9 8.15 2.0E−04 69.8NUE572 9321.1 101.00 3.1E−02 28.83 Control 4.80 NUE572 9322.2 98.051.1E−02 25.06 NUE582 9561.1 6.90 2.6E−01 25.17 Control 78.40 NUE5829562.4 7.88 3.3E−02 42.86 NUE573 9491.1 226.63 4.7E−02 49.33 NUE5829561.2 8.95 3.0E−02 62.36 Control 151.76 Control 5.51 NUE581 9723.6125.85 9.7E−02 33.8 NUE583 9673.4 11.28 6.2E−02 75.78 NUE581 9724.599.23 7.8E−01 5.5 NUE583 9673.2 7.70 4.3E−01 20.04 NUE581 9724.9 165.352.0E−04 75.7 Control 6.41 Control 94.09 NUE585 9661.1 6.95 7.5E−02 31.13NUE582 9564.2 189.45 1.4E−01 36.42 Control 5.30 NUE582 9561.1 186.301.5E−01 34.15 NUE587 9643.2 10.20 3.2E−02 85.03 NUE582 9562.4 209.487.3E−02 50.84 NUE587 9641.3 8.23 1.8E−01 49.21 NUE582 9561.2 244.259.8E−02 75.88 Control 5.51 Control 138.88 NUE592 9744.5 9.80 1.0E−07104.2 NUE583 9673.4 222.13 4.7E−02 54.28 NUE592 9747.5 8.23 2.0E−04 71.4Control 143.97 Control 4.80 NUE585 9661.5 198.18 6.9E−02 24.22 NUE5859661.1 194.93 2.6E−01 22.18 Control 159.54 NUE587 9643.2 242.53 4.2E−0253.46 NUE587 9643.1 221.50 1.9E−01 40.16 NUE587 9642.5 169.73 7.0E−017.40 NUE587 9642.2 192.08 4.1E−01 21.54 NUE587 9641.3 268.95 3.5E−0470.18 Control 158.04 NUE592 9741.7 115.18 2.6E−01 22.4 NUE592 9744.5197.68 2.6E−07 110.1 NUE592 9747.4 118.53 1.9E−01 26.0 NUE592 9747.5169.38 1.0E−04 80.0 Control 94.09

Table 29: Analyses of plant biomass (plant fresh and dry weight) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under standard nitrogen conditions [normal or regular growthconditions (15 mM N)] as compared to control plants. “Incr.”=increment;“RGR”=relative growth rate.

TABLE 30 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (leafarea) under standard nitrogen conditions Leaf Area cm² Gene Name Event #Average p-value % increment CT11 4892.2 0.873 6.4E−03 72.49 CT11 4892.30.809 1.6E−03 59.89 CT11 4892.1 0.848 8.2E−02 67.56 Control 0.506 CT114894.2 0.474 8.2E−02 21.28 CT11 4893.2 0.763 2.7E−02 94.95 Control 0.391CT27 5033.8 0.645 3.8E−02 81.02 CT27 5033.5 0.482 8.8E−02 35.52 Control0.356 CT27 5033.7 0.495 6.9E−02 26.56 CT27 5035.2 0.751 1.2E−02 92.06CT27 5031.4 0.582 1.3E−05 48.90 CT27 5033.6 0.602 1.6E−02 53.79 CT275033.4 0.575 1.3E−02 47.12 CT27 5033.8 0.528 6.0E−03 34.95 CT27 5033.50.446 5.8E−02 14.08 Control 0.391 CT6 4941.4 0.551 1.5E−04 40.90 Control0.391 CT75 4872.5 0.506 1.0E−01 29.29 CT75 4874.4 0.529 3.1E−02 35.35Control 0.391 CT76 5044.6 0.702 8.6E−03 38.82 CT76 5041.5 0.674 2.0E−0133.22 CT76 5041.7 0.596 1.4E−01 17.83 CT76 5043.5 1.093 2.1E−05 116.09CT76 5041.6 0.779 4.9E−02 53.91 CT76 5041.9 0.749 4.0E−03 48.14 Control0.506 CT76 5044.6 0.663 1.6E−02 86.32 CT76 5041.5 0.904 2.4E−03 153.77CT76 5043.5 0.850 1.8E−03 138.87 CT76 5041.6 0.528 2.3E−02 48.40 CT765041.9 0.696 6.3E−03 95.60 Control 0.356 CT81 4992.1 0.804 7.1E−02 58.98CT81 4992.2 0.778 3.4E−03 53.73 Control 0.506 NUE206 6732.5 0.7073.6E−02 21.77 Control 0.580 NUE208 8354.8 0.765 1.1E−02 31.82 NUE2088354.5 0.727 1.3E−01 25.27 NUE208 8355.3 0.763 5.7E−02 31.55 Control0.580 NUE209 8192.14 0.458 2.2E−01 43.17 NUE209 8191.5 0.430 7.0E−0234.44 Control 0.320 NUE210 8201.3 0.485 1.1E−02 51.70 Control 0.320NUE210 8202.1 0.414 1.3E−02 30.69 NUE210 6755.3 0.474 5.8E−02 49.68Control 0.316 NUE210 8201.2 0.275 1.5E−02 35.08 Control 0.204 NUE2118265.1 0.253 7.2E−02 24.01 NUE211 8263.5 0.370 4.7E−04 81.74 Control0.204 NUE212 8335.1 0.332 6.3E−02 62.71 NUE212 8334.1 0.277 5.9E−0336.04 NUE212 8331.4 0.268 1.1E−01 31.45 Control 0.204 NUE212 8335.20.490 1.2E−02 53.10 Control 0.320 NUE212 8332.1 0.390 7.7E−03 23.33NUE212 8334.1 0.420 2.1E−03 32.80 NUE212 8331.4 0.430 2.3E−03 35.83Control 0.316 NUE221 9801.10 0.47 5.6E−01 8.7 NUE221 9802.8 0.59 1.0E−0238.4 NUE221 9806.1 0.72 1.0E−05 68.8 Control 0.43 NUE224 9002.2 0.5251.3E−02 19.12 NUE224 9001.3 0.542 9.6E−02 22.97 Control 0.441 NUE2279851.2 0.531 1.9E−01 22.50 NUE227 9853.1 0.628 2.1E−02 44.84 NUE2279852.3 0.550 6.8E−02 26.84 Control 0.433 NUE228 10092.2 0.691 2.5E−0122.23 NUE228 10093.3 0.759 3.6E−02 34.30 NUE228 10093.1 0.646 1.7E−0114.35 Control 0.565 NUE229 8864.2 0.430 2.4E−02 21.48 Control 0.354NUE230 9154.2 0.559 1.3E−01 26.94 NUE230 9151.2 0.519 8.3E−02 17.74Control 0.441 NUE231 10631.3 0.46 5.9E−01 8.0 NUE231 10632.2 0.502.7E−01 16.3 NUE231 10633.3 0.74 3.9E−06 72.3 Control 0.43 NUE23310174.3 0.512 7.7E−04 87.53 NUE233 10174.1 0.735 5.1E−06 169.13 NUE23310173.7 0.398 2.9E−02 45.70 Control 0.273 NUE233 10174.1 0.820 1.2E−0435.44 Control 0.606 NUE235 9694.2 0.516 1.0E−01 17.08 NUE235 9694.30.578 2.6E−02 31.06 Control 0.441 NUE237 9651.1 0.710 3.7E−03 61.05NUE237 9654.4 0.566 1.9E−02 28.50 NUE237 9654.1 0.634 1.4E−01 43.87Control 0.441 NUE241 9631.3 0.670 6.8E−04 54.67 NUE241 9632.5 0.6261.2E−01 44.49 NUE241 9632.4 0.601 1.1E−03 38.64 Control 0.433 NUE2429214.1 0.726 5.0E−02 18.36 Control 0.613 NUE242 9212.1 0.542 4.1E−0242.31 NUE242 9213.4 0.518 1.3E−02 35.92 Control 0.381 NUE244 9061.50.473 4.8E−03 33.52 Control 0.354 NUE246 9033.8 0.740 3.2E−03 51.11NUE246 9034.1 0.582 6.5E−02 18.92 NUE246 9031.1 0.572 6.9E−02 16.87Control 0.490 NUE248 8981.5 0.803 7.2E−02 55.67 Control 0.516 NUE2509132.1 0.744 8.5E−02 68.79 NUE250 9133.2 0.528 2.0E−02 19.79 NUE2509132.2 0.517 1.8E−01 17.26 NUE250 9134.1 0.525 1.8E−01 19.17 Control0.441 NUE250 9134.1 0.444 4.1E−03 25.38 Control 0.354 NUE251 10181.30.599 1.1E−02 119.32 NUE251 10183.2 0.467 2.4E−02 71.22 NUE251 10183.10.408 2.7E−02 49.61 NUE251 10181.1 0.397 7.3E−03 45.35 Control 0.273NUE251 10181.3 0.600 6.2E−02 22.85 Control 0.488 NUE251 10183.2 0.7132.9E−02 17.75 Control 0.606 NUE256 10063.4 0.479 4.6E−03 75.40 NUE25610064.1 0.707 1.5E−04 159.05 NUE256 10061.2 0.601 9.0E−06 120.20 NUE25610062.4 0.588 1.2E−03 115.54 NUE256 10063.2 0.507 3.2E−03 85.58 Control0.273 NUE268 8996.5 0.730 6.1E−02 39.46 Control 0.523 NUE269 9103.30.448 8.1E−02 23.13 Control 0.364 NUE512 9284.2 0.531 4.8E−02 45.85NUE512 9282.3 0.748 1.2E−04 105.28 NUE512 9284.4 0.442 5.3E−02 21.37Control 0.364 NUE514 9404.1 0.796 1.2E−04 108.76 NUE514 9402.2 0.4492.1E−01 17.88 NUE514 9403.2 0.452 2.3E−01 18.65 Control 0.381 NUE5159712.6 0.49 3.5E−01 14.0 NUE515 9713.6 0.66 5.0E−04 53.4 Control 0.43NUE516 9291.1 0.516 2.0E−01 16.98 NUE516 9291.4 0.639 6.0E−04 45.10Control 0.441 NUE520 9771.4 0.465 1.6E−02 70.36 NUE520 9771.7 0.4821.4E−02 76.62 NUE520 9771.2 0.415 2.7E−03 51.98 NUE520 9771.3 0.3601.5E−02 32.02 Control 0.273 NUE521 9363.4 0.716 4.6E−03 75.12 Control0.409 NUE521 9361.2 0.525 4.8E−02 37.71 NUE521 9363.4 0.582 2.5E−0252.79 Control 0.381 NUE523 9412.1 0.752 2.0E−02 45.74 Control 0.516NUE523 9412.5 0.526 5.2E−02 38.12 NUE523 9414.2 0.487 3.3E−02 27.79Control 0.381 NUE531 10083.1 0.809 7.9E−03 43.12 NUE531 10082.2 0.7053.0E−01 24.67 NUE531 10081.4 0.900 1.5E−02 59.14 NUE531 10081.5 0.8663.8E−02 53.10 Control 0.565 NUE531 10081.4 0.789 7.0E−02 30.24 NUE53110081.5 0.816 2.0E−02 34.70 Control 0.606 NUE535 9084.2 0.430 1.9E−0257.65 NUE535 9083.1 0.822 4.7E−03 201.24 NUE535 9084.4 0.436 1.4E−0359.81 NUE535 9082.1 0.381 1.6E−01 39.64 Control 0.273 NUE537 9391.10.526 8.2E−02 44.39 NUE537 9393.2 0.459 7.1E−02 26.03 NUE537 9394.40.471 3.3E−02 29.36 NUE537 9391.2 0.575 1.1E−02 57.79 NUE537 9393.30.733 5.0E−03 101.21 Control 0.364 NUE539 10101.5 0.510 3.6E−02 86.79NUE539 10103.5 0.432 1.6E−02 58.38 NUE539 10101.2 0.638 3.5E−04 133.70NUE539 10101.7 0.641 6.5E−03 134.76 Control 0.273 NUE542 9333.2 0.5354.4E−04 46.83 NUE542 9331.3 0.455 7.7E−02 24.84 NUE542 9332.1 0.4112.9E−01 12.88 Control 0.364 NUE543 10052.3 0.556 2.0E−01 28.45 NUE54310051.6 0.530 2.6E−02 22.32 Control 0.433 NUE543 10051.2 0.759 7.7E−0234.20 NUE543 10051.6 0.682 1.0E−01 20.71 Control 0.565 NUE544 9763.30.596 1.8E−03 36.70 Control 0.436 NUE548 9091.1 0.783 1.2E−02 27.78Control 0.613 NUE550 9144.3 0.436 4.0E−02 19.74 Control 0.364 NUE5509143.1 0.558 1.4E−02 35.96 Control 0.410 NUE550 9141.3 0.622 2.0E−0118.74 NUE550 9142.2 0.664 5.7E−03 26.77 Control 0.523 NUE551 9351.10.566 7.6E−02 15.59 Control 0.490 NUE560 9424.3 0.537 8.5E−02 31.22NUE560 9422.1 0.581 2.0E−04 41.90 Control 0.409 NUE564 9244.1 0.5207.9E−02 22.64 Control 0.424 NUE564 9242.3 0.415 5.6E−02 13.97 NUE5649243.2 0.469 7.6E−02 28.83 NUE564 9242.2 0.585 4.5E−04 60.72 Control0.364 NUE567 9263.2 0.510 2.5E−02 40.15 NUE567 9261.3 0.415 3.4E−0113.94 NUE567 9261.2 0.400 6.0E−01 9.89 NUE567 9263.3 0.453 3.9E−01 24.33Control 0.364 NUE568 9471.3 0.613 4.5E−04 60.34 NUE568 9472.2 0.6893.5E−03 80.16 Control 0.382 NUE569 9381.2 0.576 1.6E−02 35.83 NUE5699381.3 0.507 1.7E−01 19.49 Control 0.424 NUE571 9304.2 0.752 4.6E−0383.90 NUE571 9301.1 0.623 1.4E−01 52.23 NUE571 9303.2 0.545 6.6E−0433.32 NUE571 9302.1 0.574 1.3E−04 40.39 NUE571 9302.3 0.524 9.1E−0228.11 Control 0.409 NUE571 9301.4 0.492 6.1E−02 16.00 Control 0.424NUE573 9491.1 0.511 1.5E−02 33.50 NUE573 9491.4 0.539 2.3E−01 40.86Control 0.382 NUE576 9794.1 0.503 1.2E−01 16.10 NUE576 9793.3 0.6419.9E−03 48.00 Control 0.433 NUE578 9524.3 0.551 3.2E−04 43.94 NUE5789524.1 0.477 2.2E−01 24.73 Control 0.382 NUE579 9701.3 0.481 8.3E−0218.67 Control 0.406 NUE580 9554.4 0.483 2.0E−02 26.39 Control 0.382NUE581 9723.6 0.53 1.3E−01 22.7 NUE581 9724.9 0.74 2.9E−06 73.4 Control0.43 NUE582 9562.4 0.567 2.6E−02 28.69 NUE582 9561.2 0.649 3.4E−02 47.37Control 0.441 NUE583 9673.4 1.065 4.9E−03 75.90 NUE583 9673.2 0.7738.5E−02 27.63 Control 0.606 NUE586 9751.6 0.551 3.4E−02 26.32 NUE5869751.7 0.628 2.7E−02 44.06 NUE586 9752.4 0.479 8.0E−02 9.73 NUE5869752.1 0.609 2.0E−02 39.56 Control 0.436 NUE587 9643.2 0.780 4.2E−0392.27 NUE587 9641.3 0.503 1.9E−01 23.98 Control 0.406 NUE592 9744.5 0.891.0E−10 106.7 NUE592 9747.5 0.59 1.0E−02 38.7 Control 0.43

Table 30: Analyses of plant biomass (leaf area) of transgenic plantsoverexpressing the exogenous polynucleotides of some embodiments of theinvention (using the cloned or synthetic genes listed in Table 23 above)under the regulation of a constitutive promoter (35S) when grown understandard nitrogen conditions [normal or regular growth conditions (15 mMN)] as compared to control plants. “Incr.”=increment; “RGR”=relativegrowth rate.

The genes presented in Table 31 hereinbelow, have improved plant NUEsince they produced larger root biomass when grown under standardnitrogen growth conditions, compared to control plants. Plants producinglarger root biomass have better possibilities to absorb larger amount ofnitrogen from soil.

Table 31 depicts analyses of root performance (root length and coverage)when grown under standard nitrogen conditions [normal or regular growthconditions (15 mM N)] in plants overexpressing the polynucleotides ofsome embodiments of the invention under the regulation of a constitutivepromoter (35S). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 31 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved root performance(root length and coverage) under standard nitrogen conditions RootsLength [cm] Roots Coverage_[cm²] Gene Name Event # Average p-value %incr. Average p-value % incr. CT27 5033.6 3.341 1.8E−01 18.64 2.8791.5E−01 31.17 CT27 5033.4 3.362 1.6E−02 19.36 2.461 5.3E−01 12.11Control 2.817 2.195 CT75 4873.4 4.223 8.1E−02 13.74 3.136 8.5E−01 2.38CT75 4873.3 5.290 9.1E−03 42.48 4.560 7.9E−02 48.88 Control 3.713 3.063CT76 5043.5 4.908 7.0E−02 23.17 7.927 1.5E−02 101.19 CT76 5041.6 4.2864.2E−01 7.57 5.216 3.7E−01 32.41 CT76 5041.9 4.051 7.2E−01 1.67 4.8093.9E−02 22.05 Control 3.984 3.940 CT76 5041.5 4.273 5.4E−04 51.72 5.3772.8E−03 144.94 CT76 5043.5 2.837 9.1E−01 0.73 3.200 5.7E−03 45.76Control 2.817 2.195 NUE206 6731.2 3.619 6.8E−02 28.05 3.376 3.5E−0260.66 NUE206 6732.7 3.311 6.7E−02 17.14 2.577 1.3E−01 22.63 NUE2066732.5 3.516 3.1E−02 24.42 2.894 1.3E−01 37.69 NUE206 6732.1 3.3472.0E−02 18.43 2.575 1.2E−01 22.53 Control 2.826 2.102 NUE206 6731.24.088 4.8E−02 36.80 5.469 3.2E−02 109.44 NUE206 6732.5 4.106 1.2E−0337.41 5.064 5.2E−03 93.92 NUE206 6732.15 3.669 9.1E−02 22.78 4.0312.4E−02 54.36 Control 2.988 2.611 NUE208 8351.3 3.930 2.9E−03 39.053.339 8.4E−03 58.86 NUE208 8354.4 3.385 1.8E−01 19.76 2.828 1.3E−0234.57 Control 2.826 2.102 NUE208 8355.3 3.393 6.0E−01 8.41 3.528 8.5E−0248.07 Control 3.130 2.382 NUE208 8355.3 3.600 6.6E−02 20.47 3.9698.6E−02 51.99 Control 2.988 2.611 NUE212 8332.2 4.896 6.1E−02 14.165.444 2.0E−02 27.45 Control 4.289 4.272 NUE221 9802.8 3.72 4.9E−01 12.1Control 3.32 NUE223 9613.1 4.376 9.8E−02 18.05 5.480 4.0E−02 36.61NUE223 9612.3 4.426 8.7E−02 19.41 5.228 4.5E−02 30.32 Control 3.7074.012 NUE230 9152.4 4.034 9.1E−03 17.77 3.441 1.1E−01 40.34 Control3.425 2.452 NUE231 10631.3 4.45 5.3E−02 34.2 NUE231 10632.2 4.31 7.8E−0314.7 4.64 2.3E−02 40.0 NUE231 10633.3 4.84 7.0E−04 28.9 6.30 1.2E−0690.1 Control 3.76 3.32 NUE233 10174.3 3.326 1.2E−01 16.40 2.879 3.3E−0245.78 NUE233 10174.1 4.581 3.9E−04 60.32 5.392 3.2E−04 173.01 NUE23310173.5 4.414 4.8E−04 54.47 2.865 1.1E−02 45.06 NUE233 10172.5 3.5813.5E−02 25.30 2.957 4.8E−03 49.73 NUE233 10173.7 3.100 4.5E−01 8.482.613 1.9E−01 32.30 Control 2.858 1.975 NUE233 10174.1 4.375 1.3E−0230.08 3.884 1.6E−01 18.86 NUE233 10173.5 4.755 7.1E−04 41.36 4.7462.3E−02 45.25 Control 3.363 3.268 NUE233 10174.1 4.357 5.8E−04 21.634.698 6.0E−03 39.12 Control 3.582 3.377 NUE237 9654.4 3.928 8.6E−01−1.32 4.729 1.0E−02 21.38 NUE237 9654.1 4.951 4.0E−02 24.37 6.0357.3E−02 54.90 Control 3.981 3.896 NUE237 9654.1 3.831 1.8E−01 11.854.235 2.3E−02 49.30 Control 3.425 2.837 NUE239 9191.2 4.379 2.1E−0227.86 5.300 3.4E−02 86.84 Control 3.707 4.012 NUE241 9631.3 4.0104.1E−01 5.60 3.785 1.4E−01 23.13 NUE241 9632.5 5.084 4.2E−04 33.88 6.2072.3E−03 101.95 NUE241 9632.3 4.507 1.7E−01 18.69 4.237 2.3E−01 37.86Control 3.797 3.074 NUE242 9213.4 4.696 5.9E−02 20.84 5.038 1.3E−0128.12 Control 3.886 3.933 NUE246 9033.8 4.534 1.2E−01 14.09 5.5226.1E−02 50.92 Control 3.974 3.659 NUE251 10181.3 3.824 2.7E−02 33.823.356 9.5E−03 69.93 NUE251 10183.2 3.635 3.7E−02 27.20 3.158 3.6E−0259.92 NUE251 10183.1 3.726 6.2E−02 30.37 3.075 7.4E−02 55.70 Control2.858 1.975 NUE251 10181.3 4.406 1.5E−03 30.98 4.945 2.9E−02 51.35Control 3.363 3.268 NUE256 10063.4 4.545 1.0E−02 59.03 4.513 1.7E−02128.53 NUE256 10064.1 3.444 8.2E−02 20.53 3.655 3.0E−04 85.07 NUE25610061.2 3.398 6.3E−02 18.90 3.772 7.5E−05 91.01 NUE256 10063.2 3.4642.0E−01 21.21 3.617 7.2E−02 83.13 Control 2.858 1.975 NUE256 10061.34.011 8.5E−02 11.96 3.500 7.6E−01 3.66 Control 3.582 3.377 NUE269 9104.14.116 9.0E−02 26.30 3.527 2.2E−01 28.77 Control 3.259 2.739 NUE5129284.3 4.178 3.9E−01 14.99 3.222 6.3E−01 12.38 NUE512 9282.3 3.6838.6E−01 1.36 4.173 1.7E−02 45.56 NUE512 9284.4 5.110 6.5E−04 40.66 3.9873.4E−02 39.06 Control 3.633 2.867 NUE513 9681.6 4.639 2.5E−02 25.145.087 1.9E−01 26.81 Control 3.707 4.012 NUE513 9683.5 5.331 3.4E−0220.24 4.960 9.1E−01 1.26 Control 4.433 4.898 NUE513 9683.5 5.216 1.6E−0431.26 4.402 2.2E−01 20.31 Control 3.974 3.659 NUE514 9403.2 5.8892.1E−04 54.38 6.448 1.4E−03 101.05 Control 3.815 3.207 NUE515 9712.6.3.87 3.4E−01 16.6 NUE515 9713.6. 4.19 1.3E−01 26.3 Control 3.32 NUE5209771.4 3.480 6.1E−02 21.77 3.356 7.8E−02 69.94 NUE520 9771.2 3.4875.7E−02 22.01 4.241 1.3E−02 114.72 NUE520 9772.1 3.382 2.6E−01 18.353.936 4.7E−02 99.31 NUE520 9771.3 3.284 2.0E−01 14.93 3.728 4.4E−0388.78 Control 2.858 1.975 NUE523 9414.2 5.238 9.7E−02 18.15 4.9129.9E−01 0.28 Control 4.433 4.898 NUE523 9412.5 4.589 2.1E−02 24.78 3.8205.5E−01 11.95 NUE523 9414.2 4.983 3.6E−02 35.48 4.310 1.6E−01 26.28Control 3.678 3.413 NUE523 9412.5 3.997 5.3E−01 4.78 4.391 1.4E−02 36.91NUE523 9414.2 4.386 7.8E−02 14.98 4.588 1.8E−02 43.04 Control 3.8153.207 NUE531 10083.3 4.413 5.7E−02 33.45 3.781 2.2E−01 24.13 NUE53110081.4 3.857 3.6E−02 16.65 4.484 3.8E−02 47.23 NUE531 10081.5 4.3773.6E−02 32.38 4.698 9.9E−02 54.25 Control 3.306 3.046 NUE531 10083.23.852 7.3E−02 14.51 3.992 6.0E−02 22.18 Control 3.363 3.268 NUE53110083.3 4.256 9.0E−02 18.79 4.023 2.8E−01 19.16 NUE531 10081.4 4.0665.2E−02 13.49 4.864 3.7E−02 44.06 NUE531 10081.5 4.240 1.7E−02 18.374.905 8.1E−02 45.26 Control 3.582 3.377 NUE535 9084.2 3.244 2.5E−0113.51 3.005 9.7E−02 52.16 NUE535 9083.1 3.300 2.3E−01 15.49 3.2637.1E−02 65.20 Control 2.858 1.975 NUE537 9393.3 3.603 8.9E−01 −0.834.005 4.8E−02 39.70 Control 3.633 2.867 NUE538 9782.1 3.805 9.8E−01 0.223.850 7.9E−02 25.26 Control 3.797 3.074 NUE538 9784.4 3.674 7.5E−0216.79 2.729 4.6E−01 12.64 NUE538 9783.4 4.332 6.1E−03 37.70 3.5714.5E−02 47.38 Control 3.146 2.423 NUE539 10103.5 3.228 2.0E−01 12.962.727 1.5E−01 38.07 NUE539 10101.7 3.736 1.6E−02 30.72 3.446 8.3E−0374.48 Control 2.858 1.975 NUE543 10052.3 4.801 3.8E−03 26.44 4.3734.7E−02 42.28 Control 3.797 3.074 NUE543 10051.1 4.064 2.9E−02 22.914.493 6.0E−02 47.51 NUE543 10052.3 4.003 1.1E−01 21.06 3.953 2.1E−0129.78 Control 3.306 3.046 NUE544 9764.1 3.603 9.6E−02 14.52 3.0752.3E−02 26.92 NUE544 9763.3 3.953 1.8E−02 25.66 3.096 3.0E−02 27.77Control 3.146 2.423 NUE550 9141.3 4.453 4.6E−01 8.58 5.445 5.9E−02 35.65Control 4.101 4.014 NUE551 9354.3 4.584 9.0E−02 23.66 5.375 1.3E−0133.99 Control 3.707 4.012 NUE566 9512.2 5.004 3.0E−02 18.17 5.7412.3E−01 27.49 NUE566 9512.1 4.859 1.5E−01 14.75 5.807 3.0E−01 28.96Control 4.234 4.503 NUE568 9471.3 3.796 2.4E−01 10.82 3.883 8.9E−0236.89 NUE568 9472.2 4.025 1.8E−01 17.50 4.944 9.7E−03 74.30 NUE5689462.3 4.519 4.8E−02 31.93 4.354 5.0E−02 53.47 Control 3.425 2.837NUE570 9311.4 4.279 3.0E−01 17.77 4.349 5.4E−02 51.70 NUE570 9314.43.692 8.1E−01 1.63 4.044 2.8E−02 41.05 Control 3.633 2.867 NUE571 9304.23.841 7.5E−01 4.43 4.423 3.7E−01 29.60 NUE571 9301.1 4.444 6.6E−02 20.845.020 1.1E−01 47.11 Control 3.678 3.413 NUE573 9491.1 3.948 7.6E−0215.26 3.887 4.8E−02 37.01 Control 3.425 2.837 NUE574 10363.4 4.0805.6E−02 13.90 4.326 2.0E−01 28.12 NUE574 10366.1 4.893 3.3E−02 36.585.262 4.3E−02 55.84 Control 3.582 3.377 NUE576 9792.4 4.284 1.5E−0112.82 4.322 2.3E−02 40.63 Control 3.797 3.074 NUE579 9701.3 3.9659.9E−02 26.03 3.899 4.7E−02 60.90 NUE579 9703.3 3.542 1.0E−01 12.602.797 4.7E−01 15.43 Control 3.146 2.423 NUE580 9553.2 4.418 4.7E−0328.99 3.784 7.3E−02 33.40 NUE580 9551.4 4.239 5.1E−02 23.75 4.2821.5E−01 50.96 NUE580 9554.4 4.158 8.9E−02 21.41 3.902 7.1E−02 37.54Control 3.425 2.837 NUE582 9562.4 4.115 5.1E−01 3.38 4.951 7.6E−02 27.06Control 3.981 3.896 NUE583 9673.4 4.319 2.2E−03 20.56 5.635 4.1E−0366.89 NUE583 9673.2 3.739 5.9E−01 4.36 4.595 1.5E−01 36.10 Control 3.5823.377 NUE586 9752.1 6.394 2.6E−05 49.10 7.681 6.9E−05 79.81 Control4.289 4.272 NUE586 9751.1 3.847 9.6E−02 22.28 3.171 2.3E−01 30.87 NUE5869751.7 4.956 9.5E−03 57.53 4.510 8.1E−02 86.12 NUE586 9752.2 4.5382.3E−04 44.25 3.478 1.6E−03 43.54 NUE586 9751.3 4.047 2.8E−01 28.623.090 3.3E−01 27.54 NUE586 9752.1 4.629 3.9E−02 47.13 4.232 1.4E−0174.66 Control 3.146 2.423 NUE587 9643.2 3.473 3.4E−01 10.38 3.6534.2E−02 50.77 Control 3.146 2.423 NUE593 10394.2 4.033 4.5E−02 21.963.941 3.7E−02 29.39 NUE593 10393.2 3.719 6.3E−02 12.48 3.544 1.8E−0116.38 Control 3.306 3.046

Table 31: Analyses of root performance (root length and coverage) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under standard nitrogen conditions [normal or regular growthconditions (15 mM N)] as compared to control plants. “Incr.”=increment;“RGR”=relative growth rate.

The genes presented in Table 32, hereinbelow, have improved plant growthrate when grown under standard nitrogen growth conditions, compared tocontrol plants. Faster growth was observed when growth rate of leaf areaand root length and coverage was measured.

Table 32 depicts analyses of leaf area, root length and root coveragegrowth rate when grown under standard nitrogen conditions [normal orregular growth conditions (15 mM N)] in plants overexpressing thepolynucleotides of some embodiments of the invention under theregulation of a constitutive promoter (35S). Evaluation of each gene wasperformed by testing the performance of several events. Some of thegenes were evaluated in more than one tissue culture assay and theresults obtained were repeated. Event with p-value<0.1 was consideredstatistically significant.

TABLE 32 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved growth rate understandard nitrogen conditions RGR Of Leaf Area RGR Of Roots Coverage RGROf Roots Length Gene Event p- % p- % p- % Name # Ave. value incr. Ave.value incr. Ave. value incr. CT11 4892.2 0.093 2.4E−04 76.02 0.5847.8E−02 28.88 0.428 1.0E−01 17.45 CT11 4892.3 0.082 1.3E−03 55.84 0.5243.6E−01 15.68 0.397 4.0E−01 8.75 CT11 4892.1 0.085 6.5E−03 61.71 0.4906.8E−01 8.24 0.385 6.7E−01 5.43 Control 0.053 0.453 0.365 CT11 4894.20.049 2.0E−02 30.32 CT11 4893.2 0.078 3.6E−05 107.01 0.550 3.4E−02 52.790.395 2.0E−01 21.56 Control 0.037 0.360 0.325 CT27 5033.6 0.040 1.6E−0122.17 0.347 4.1E−02 40.85 0.325 1.9E−02 36.08 CT27 5033.4 0.047 3.2E−0240.54 0.287 3.5E−01 16.49 0.296 5.9E−02 24.30 CT27 5033.8 0.063 5.9E−0590.98 0.357 1.0E−01 44.71 0.251 7.5E−01 5.19 CT27 5033.5 0.049 3.7E−0348.72 0.248 7.8E−01 3.85 Control 0.033 0.247 0.238 CT27 5033.7 0.0491.8E−02 31.77 CT27 5035.2 0.079 8.4E−07 111.02 0.402 5.8E−01 11.56 0.3437.1E−01 5.73 CT27 5031.4 0.053 1.5E−03 41.85 CT27 5033.6 0.058 4.6E−0453.91 0.513 3.1E−02 42.39 0.357 5.4E−01 10.03 CT27 5033.4 0.060 2.2E−0559.59 0.547 3.2E−02 51.81 0.416 9.9E−02 28.01 CT27 5033.8 0.055 3.8E−0447.37 CT27 5033.5 0.047 4.0E−02 25.25 Control 0.037 0.360 0.325 CT64943.1 0.048 6.3E−02 27.13 0.378 8.1E−01 4.79 Control 0.037 0.360 0.325CT75 4872.5 0.055 2.1E−03 47.90 CT75 4874.4 0.053 3.2E−03 42.55 CT754874.7 0.054 6.7E−03 44.21 0.368 9.2E−01 2.17 CT75 4873.3 0.052 1.3E−0239.84 0.518 4.3E−02 43.19 0.415 1.1E−01 27.69 Control 0.037 0.360 0.325CT76 5044.6 0.072 2.6E−02 36.36 CT76 5041.5 0.068 29.56 0.685 2.8E−0251.34 0.403 4.7E−01 10.41 CT76 5041.7 0.058 9.50 CT76 5043.5 0.1141.4E−07 115.19 0.961 9.5E−06 112.17 0.506 2.2E−03 38.75 CT76 5041.60.082 7.3E−03 55.95 0.626 9.8E−02 38.15 0.448 5.4E−02 22.83 CT76 5041.90.079 3.8E−03 49.27 0.571 9.6E−02 25.96 0.378 7.0E−01 3.74 Control 0.0530.453 0.365 CT76 5044.6 0.064 2.7E−05 92.67 0.283 4.2E−01 14.80 0.2921.2E−01 22.42 CT76 5041.5 0.094 9.7E−12 182.48 0.635 9.1E−08 157.510.384 2.8E−05 61.08 CT76 5041.7 0.042 3.4E−02 25.46 CT76 5043.5 0.0911.1E−11 174.29 0.394 2.7E−03 59.72 0.300 4.2E−02 25.74 CT76 5041.6 0.0527.3E−05 55.70 0.287 3.9E−01 16.43 0.271 3.6E−01 13.45 CT76 5041.9 0.0664.5E−07 100.31 0.408 1.2E−03 65.59 0.317 9.7E−03 33.04 Control 0.0330.247 0.238 NUE206 6731.2 0.396 1.6E−03 78.33 0.369 8.5E−03 44.35 NUE2066732.1 0.297 1.5E−01 33.53 0.333 4 .9E−02 30.52 Control 0.036 0.2220.256 NUE206 6731.2 0.063 6.3E−01 7.32 0.650 3.1E−05 125.39 0.4354.9E−05 65.80 NUE206 6732.5 0.074 9.5E−02 26.57 0.579 4.4E−06 100.850.389 8.9E−05 48.32 NUE206 6732.2 0.059 9.2E−01 1.58 0.464 1.9E−03 60.760.365 4.6E−03 39.21 Control 0.058 0.288 0.262 NUE208 8351.3 0.033 0.3891.4E−03 74.92 0.397 1.9E−04 55.33 Control 0.036 0.222 0.256 NUE2088354.8 0.041 24.69 0.422 3.7E−02 51.73 0.393 8.2E−02 35.05 NUE208 8355.30.037 13.69 0.418 3.5E−02 50.07 0.331 4.7E−01 13.65 Control 0.033 0.2780.291 NUE208 8354.8 0.076 9.1E−02 30.54 NUE208 8354.5 0.073 1.8E−0124.95 0.305 7.4E−01 5.73 0.276 6.4E−01 5.23 NUE208 8355.3 0.079 5.8E−0234.69 0.460 7.5E−03 59.54 0.362 3.1E−03 37.83 NUE208 8351.5 0.0599.0E−01 1.94 0.312 6.2E−01 8.27 0.315 9.1E−02 19.92 Control 0.058 0.2880.262 NUE209 8192.1 0.045 1.4E−01 36.98 0.450 1.9E−02 61.65 0.3681.4E−01 26.60 NUE209 8191.5 0.044 7.8E−02 34.71 0.295 7 .7E−01 6.00Control 0.033 0.278 0.291 NUE209 8191.2 0.041 4.5E−02 31.45 0.3304.3E−01 12.88 0.335 4.4E−01 12.06 NUE209 8192.1 0.035 3.6E−01 14.35NUE209 8192.1 0.044 2.3E−02 40.98 NUE209 8191.5 0.040 5.9E−02 30.43NUE209 8192.1 0.034 5.7E−01 9.26 Control 0.031 0.292 0.299 NUE209 8192.10.345 19.74 0.365 1.4E−03 39.07 NUE209 8192.1 0.487 9.6E−03 68.94 0.3772.5E−02 43.59 NUE209 8191.3 0.083 4.7E−02 41.92 Control 0.058 0.2880.262 NUE210 8202.1 0.049 3.9E−02 48.76 NUE210 8201.3 0.049 9.7E−0350.64 Control 0.033 0.278 0.291 NUE210 8202.1 0.042 3.6E−02 34.98 0.4034.8E−02 37.90 0.345 3.7E−01 15.40 NUE210 6755.3 0.048 4.6E−03 55.180.423 1.9E−02 44.72 0.441 1.1E−02 47.78 Control 0.031 0.292 0.299 NUE2118263.5 0.038 7.6E−05 89.32 0.191 8.9E−02 58.43 0.215 5.7E−02 53.44Control 0.020 0.121 0.140 NUE212 8335.1 0.036 2.7E−03 82.84 0.2165.8E−02 79.29 0.235 4.4E−02 68.20 NUE212 8334.1 0.029 6.2E−03 46.650.131 7.9E−01 8.85 0.159 6.3E−01 13.62 NUE212 8331.4 0.026 9.1E−02 31.280.153 7.1E−01 9.70 Control 0.020 0.121 0.140 NUE212 8335.2 0.049 1.5E−0248.76 0.314 5.6E−01 12.68 Control 0.033 0.278 0.291 NUE212 8332.2 0.0468.1E−01 3.53 0.656 4.5E−02 27.59 0.507 1.2E−01 19.17 Control 0.044 0.5140.426 NUE212 8332.1 0.039 5.5E−02 26.08 NUE212 8334.1 0.044 5.7E−0341.96 0.316 6.2E−01 8.13 0.361 2.1E−01 20.73 NUE212 8331.4 0.041 3.2E−0231.53 0.307 8.8E−01 2.62 Control 0.031 0.292 0.299 NUE222 8851.3 0.0466.7E−02 41.29 0.365 2.2E−01 31.13 0.291 1.0E+00 −0.102 Control 0.0330.278 0.291 NUE223 9613.1 0.659 3.7E−02 36.07 0.449 9.4E−02 19.43Control 0.068 0.484 0.376 NUE224 9002.2 0.052 5.5E−02 23.49 NUE2249001.3 0.055 2.7E−02 30.04 Control 0.042 0.445 0.370 NUE224 9001.3 0.0463.2E−02 28.75 Control 0.036 0.279 0.316 NUE227 9851.2 0.058 2.8E−0235.17 NUE227 9853.1 0.064 3.6E−03 49.89 Control 0.043 0.349 0.360 NUE22810093 0.078 2.9E−02 39.70 0.355 3.25 0.328 16.65 NUE228 10093 0.0672.1E−01 20.98 0.301 7.24 Control 0.056 0.344 0.281 NUE229 8864.2 0.0453.9E−02 26.44 Control 0.036 0.279 0.316 NUE230 9154.2 0.057 2.1E−0235.25 0.461 3.61 NUE230 9151.2 0.055 1.7E−02 29.70 Control 0.042 0.4450.370 NUE230 9152.4 0.047 1.7E−01 29.87 0.378 7.6E−02 35.74 0.3287.2E−01 3.71 Control 0.036 0.279 0.316 NUE233 10174 0.051 4.1E−06 93.040.340 1.4E−02 54.67 0.311 1.7E−01 31.25 NUE233 10174 0.069 5.1E−11160.30 0.624 8.1E−09 183.57 0.399 7.2E−03 68.42 NUE233 10174 0.0303.6E−01 13.97 0.314 4.1E−02 42.81 0.344 5.6E−02 45.20 NUE233 10173 0.0321.9E−01 19.26 0.342 9.3E−03 55.25 0.324 1.1E−01 36.68 NUE233 10174 0.0391.1E−02 45.92 0.299 1.2E−01 35.80 0.262 6.4E−01 10.75 Control 0.0260.220 0.237 NUE233 10174 0.053 7.8E−01 4.51 0.451 3.0E−01 17.53 0.3815.1E−02 32.89 NUE233 10174 0.538 2.8E−02 40.15 0.407 1.2E−02 41.78Control 0.051 0.384 0.287 NUE234 9163.5 0.044 9.7E−02 22.24 NUE2349162.1 0.048 2.8E−02 32.38 0.381 7.4E−02 36.73 0.330 7.6E−01 4.39Control 0.036 0.279 0.316 NUE235 9694.2 0.054 3.7E−02 27.26 0.4756.0E−01 6.64 NUE235 9691.1 0.055 6.9E−02 31.10 0.454 8.9E−01 1.90 0.3827.4E−01 3.28 NUE235 9693.3 0.054 5.5E−02 28.28 NUE235 9694.3 0.0621.0E−03 46.88 0.583 1.9E−02 30.90 0.470 1.2E−02 27.17 Control 0.0420.445 0.370 NUE237 9651.1 0.073 7.6E−06 74.28 0.508 2.1E−01 14.12 NUE2379654.4 0.057 5.2E−03 36.61 0.553 2.5E−02 24.26 0.384 7.1E−01 3.83 NUE2379654.1 0.065 8.0E−03 53.61 0.680 5.8E−03 52.84 0.445 9.4E−02 20.30Control 0.042 0.445 0.370 NUE237 9651.1 0.047 4.6E−02 31.99 0.3319.7E−01 0.99 NUE237 9654.1 0.056 6.7E−03 58.85 0.493 9.9E−02 50.53 0.3635.2E−01 13.15 Control 0.036 0.327 0.321 NUE241 9631.3 0.066 4.8E−0452.60 0.452 5.0E−02 29.57 0.395 3.6E−01 9.92 NUE241 9632.5 0.066 9.6E−0352.36 0.733 8.9E−08 110.05 0.490 2.0E−03 36.33 NUE241 9632.3 0.0604.8E−02 39.27 0.494 5.1E−02 41.65 0.377 7.3E−01 4.72 NUE241 9632.4 0.0605.7E−03 38.59 0.428 1.8E−01 22.84 Control 0.043 0.349 0.360 NUE2429214.1 0.074 7.4E−02 25.97 0.460 8.2E−01 4.07 0.380 3.8E−01 13.95 NUE2429213.4 0.068 2.6E−01 16.65 0.588 8.2E−02 32.97 0.428 7.7E−02 28.57Control 0.059 0.442 0.333 NUE242 9212.1 0.050 4.6E−02 43.13 0.3748.2E−01 2.93 NUE242 9214.1 0.052 4.5E−02 47.68 NUE242 9213.4 0.0503.3E−02 41.82 0.504 1.6E−02 38.74 0.409 1.8E−01 20.10 Control 0.0350.363 0.341 NUE244 9061.5 0.049 2.2E−03 35.40 0.347 1.9E−01 24.39 0.3248.4E−01 2.40 Control 0.036 0.279 0.316 NUE245 1064.2 0.080 7.9E−02 33.890.622 2.6E−02 60.99 0.397 2.9E−02 26.31 Control 0.060 0.387 0.315 NUE2469033.8 0.462 7.1E−02 30.62 0.400 2.0E−01 18.15 Control 0.049 0.354 0.339NUE246 9033.6 0.053 5.9E−01 8.27 NUE246 9033.4 0.053 5.4E−01 8.70 0.5461.5E−01 30.07 0.410 2.1E−01 14.19 NUE246 9033.8 0.075 3.8E−03 52.670.658 9.3E−03 56.72 0.436 4.3E−02 21.22 NUE246 9034.1 0.060 1.3E−0122.21 NUE246 9031.1 0.057 2.3E−01 16.59 0.505 2.9E−01 20.28 0.4246.6E−02 17.89 Control 0.049 0.420 0.359 NUE248 8981.5 0.085 3.9E−0363.02 0.638 5.3E−01 11.57 Control 0.052 0.572 0.423 NUE250 9132.1 0.0781.3E−03 86.50 0.604 7.4E−02 35.67 0.386 4.46 NUE250 9132.2 0.054 4.2E−0228.86 NUE250 9134.1 0.051 1.3E−01 21.34 Control 0.042 0.445 0.370 NUE2509134.1 0.043 6.6E−02 19.98 0.329 3.0E−01 17.87 Control 0.036 0.279 0.316NUE251 10181 0.059 5.5E−06 122.34 0.396 9.1E−04 80.08 0.349 5.2E−0247.21 NUE251 10183 0.048 2.3E−04 80.31 0.369 5.8E−03 67.70 0.343 5.7E−0244.89 NUE251 10183 0.042 2.5E−03 58.23 0.366 9.1E−03 66.32 0.377 1.9E−0259.19 NUE251 10181 0.039 5.8E−03 48.78 0.249 5.2E−01 13.16 0.240 9.5E−011.48 Control 0.026 0.220 0.237 NUE251 10181 0.063 1.5E−01 23.79 0.5828.9E−03 51.43 0.381 3.8E−02 32.80 Control 0.051 0.384 0.287 NUE251 101830.072 9.1E−02 19.27 0.324 6.6E−01 3.02 Control 0.060 0.387 0.315 NUE25610063 0.050 7.6E−06 89.88 0.520 1.9E−05 136.35 0.410 6.7E−03 72.97NUE256 10064 0.076 2.0E−11 188.66 0.442 5.9E−05 100.99 0.356 3.4E−0250.21 NUE256 10061 0.056 5.0E−08 110.73 0.450 1.2E−05 104.36 0.3308.2E−02 39.37 NUE256 10062 0.054 2.3E−06 103.99 0.327 7.9E−02 48.640.272 5.3E−01 14.99 NUE256 10063 0.051 9.1E−06 93.42 0.424 2.3E−03 92.640.335 1.0E−01 41.59 Control 0.026 0.220 0.237 NUE268 8996.5 0.0727.2E−03 43.95 0.388 7.2E−01 3.95 Control 0.050 0.463 0.374 NUE512 9284.20.052 3.6E−03 48.09 0.369 3.4E−01 18.49 0.372 9.7E−02 22.41 NUE5129282.3 0.073 4.5E−09 108.17 0.514 2.9E−03 64.80 0.403 2.2E−02 32.41NUE512 9284.4 0.041 1.5E−01 18.26 0.450 2.2E−02 44.50 0.390 5.3E−0228.25 Control 0.035 0.312 0.304 NUE513 9683.5 0.511 5.7E−02 20.91Control 0.052 0.572 0.423 NUE513 9683.5 0.521 24.10 0.512 1.8E−05 42.60Control 0.049 0.420 0.359 NUE514 9404.1 0.082 7.9E−07 133.82 0.4262.2E−01 17.18 0.377 4.6E−01 10.68 NUE514 9402.2 0.044 1.7E−01 26.640.389 6.0E−01 7.20 0.356 7.5E−01 4.44 NUE514 9403.2 0.046 1.3E−01 31.040.776 1.9E−07 113.56 0.565 8.5E−05 65.81 Control 0.035 0.363 0.341NUE516 9291.1 0.054 4.3E−02 28.22 0.577 4.3E−02 29.59 0.407 3.9E−0110.04 NUE516 9291.4 0.064 8.4E−05 52.81 0.531 7.4E−02 19.37 0.3925.9E−01 6.00 Control 0.042 0.445 0.370 NUE520 9771.4 0.049 5.8E−05 87.020.408 2.7E−03 85.53 0.370 1.7E−02 56.27 NUE520 9771.7 0.050 6.3E−0588.34 0.404 5.1E−03 83.74 0.372 2.1E−02 57.16 NUE520 9771.2 0.0428.9E−04 59.66 0.491 4.1E−05 122.92 0.330 1.1E−01 39.23 NUE520 9772.10.462 5.2E−04 109.90 0.301 2.6E−01 27.23 NUE520 9771.3 0.035 3.8E−0232.37 0.454 1.3E−04 106.42 0.346 5.1E−02 46.13 NUE520 9773.1 0.0234.8E−01 −14.07 0.369 7.7E−02 67.68 0.329 2.3E−01 38.86 Control 0.0260.220 0.237 NUE520 9771.4 0.360 7.9E−01 4.93 0.352 6.6E−02 25.41 NUE5209771.7 0.434 1.9E−01 26.37 0.377 2.5E−02 34.19 Control 0.056 0.344 0.281NUE521 9362.2 0.051 4.7E−02 29.12 0.430 6.6E−01 9.02 0.375 6.4E−01 6.57NUE521 9363.4 0.073 5.8E−05 84.97 0.407 8.4E−01 3.33 0.349 9.6E−01 −0.67Control 0.040 0.394 0.351 NUE521 9361.2 0.051 3.9E−02 44.60 0.3708.9E−01 1.86 0.349 8.6E−01 2.50 NUE521 9363.4 0.059 3.0E−03 69.25 0.3133.7E−01 Control 0.035 0.363 0.341 NUE523 9412.1 0.070 2.7E−02 35.53Control 0.052 0.572 0.423 NUE523 9413.3 0.059 3.1E−02 49.30 0.4664.2E−01 18.10 0.406 NUE523 9414.2 0.052 1.4E−01 30.19 0.516 8.7E−0230.82 0.471 3.4E−01 15.59 Control 0.040 0.394 0.351 3.2E−02 34.02 NUE5239412.5 0.053 1.6E−02 51.59 0.522 3.6E−03 43.78 0.392 NUE523 9414.2 0.0468.0E−02 32.35 0.523 4.2E−03 43.92 0.394 2.8E−01 15.04 Control 0.0350.363 0.341 2.6E−01 15.82 NUE527 9202.6 0.046 1.5E−01 22.86 0.3752.0E−01 25.97 0.974 Control 0.038 0.297 0.254 2.8E−02 46.88 NUE531 100830.078 2.7E−02 40.47 0.305 NUE531 10081 0.090 3.8E−03 62.13 0.535 7.9E−0355.85 0.359 5.6E−01 8.47 NUE531 10082 0.086 1.2E−02 55.40 0.554 1.3E−0261.37 0.398 5.6E−02 27.85 Control 0.056 0.344 0.281 1.2E−02 41.80 NUE53110081 0.080 2.5E−02 32.77 0.578 49.54 0.386 NUE531 10082 0.078 2.4E−0230.60 0.581 1.5E−02 50.29 0.410 22.62 Control 0.060 0.387 0.315 7.9E−0430.44 NUE535 9084.2 0.043 1.2E−03 62.38 0.345 2.6E−02 56.73 0.277 NUE5359083.1 0.082 3.3E−09 211.11 0.388 5.6E−03 76.23 0.325 4.6E−01 17.08NUE535 9084.4 0.042 4.8E−04 59.66 0.246 5.8E−01 11.82 0.306 1.2E−0137.19 NUE535 9082.1 0.039 2.9E−02 46.36 2.2E−01 29.02 Control 0.0260.220 0.237 NUE537 9391.1 0.053 4.2E−03 51.98 0.444 6.2E−02 42.62 0.388NUE537 9394.4 0.046 3.2E−02 31.60 5.6E−02 27.48 NUE537 9391.2 0.0562.6E−04 60.43 0.385 2.0E−01 23.46 0.365 NUE537 9393.3 0.073 8.5E−07109.66 0.481 1.3E−02 54.18 0.366 1.4E−01 19.94 Control 0.035 0.312 0.3041.3E−01 20.35 NUE538 9782.1 0.071 1.0E−03 64.80 0.474 2.4E−02 36.000.424 1.3E−01 17.89 Control 0.043 0.349 0.360 NUE538 9781.4 0.0483.3E−01 15.40 0.405 5.9E−02 41.28 0.398 2.8E−02 31.83 NUE538 9783.40.046 5.4E−01 9.73 0.414 9.5E−03 44.32 0.390 2.5E−02 29.10 Control 0.0420.287 0.302 NUE539 10102 0.049 1.1E−03 83.56 0.307 1.3E−01 39.36 0.3112.0E−01 31.26 NUE539 10104 0.045 3.9E−04 69.52 0.317 6.6E−02 43.97 0.2992.4E−01 26.34 NUE539 10101 0.061 3.2E−08 129.48 0.266 3.2E−01 20.900.293 3.0E−01 23.62 NUE539 10102 0.061 1.1E−06 132.28 0.410 5.3E−0486.11 0.375 1.5E−02 58.30 Control 0.026 0.220 0.237 NUE539 10102 0.3668.7E−02 27.65 NUE539 10102 0.371 1.0E−01 29.42 Control 0.051 0.384 0.287NUE542 9333.2 0.053 2.2E−04 50.87 0.445 3.5E−02 42.68 0.400 1.7E−0231.61 NUE542 9331.3 0.045 6.9E−02 27.12 0.450 6.4E−02 44.47 0.3978.1E−02 30.56 NUE542 9332.1 0.041 2.4E−01 16.04 0.414 8.3E−02 32.840.347 2.8E−01 14.05 Control 0.035 0.312 0.304 NUE543 10052 0.060 2.7E−0239.61 0.505 8.0E−03 44.88 0.420 1.4E−01 16.75 NUE543 10052 0.058 1.6E−0233.92 0.399 3.4E−01 14.30 Control 0.043 0.349 0.360 NUE543 10051 0.0569.8E−01 0.47 0.509 2.1E−02 48.29 0.356 7.1E−02 26.63 NUE543 10051 0.0774.3E−02 39.35 0.452 1.5E−01 31.70 0.385 3.3E−02 36.96 Control 0.0560.344 0.281 NUE544 9763.3 0.060 2.7E−02 35.77 0.602 2.4E−01 17.04Control 0.044 0.514 0.426 NUE544 9764.1 0.047 3.4E−01 11.45 0.3677.3E−02 27.87 0.350 1.9E−01 15.92 NUE544 9763.3 0.046 4.0E−01 9.93 0.3581.0E−01 24.63 0.378 4.9E−02 24.94 Control 0.042 0.287 0.302 NUE5489091.3 0.058 9.7E−01 −0.55 0.489 5.6E−01 10.49 0.423 9.0E−02 26.90NUE548 9091.1 0.076 3.2E−02 29.54 0.578 1.1E−01 30.72 0.419 1.0E−0125.87 NUE548 9092.2 0.063 6.6E−01 6.64 0.686 2.4E−02 55.14 0.486 2.4E−0245.86 Control 0.059 0.442 0.333 NUE549 9343.7 0.055 5.8E−03 53.63 0.3627.2E−01 10.47 NUE549 9342.3 0.045 6.6E−02 26.67 0.330 9.7E−01 0.73 0.3703.2E−01 15.10 Control 0.036 0.327 0.321 NUE550 9143.1 0.061 6.0E−0236.03 Control 0.045 0.416 0.368 NUE550 9141.3 0.061 1.1E−01 21.68 0.6511.3E−02 40.71 0.451 8.2E−02 20.82 NUE550 9142.2 0.066 1.5E−02 31.25Control 0.050 0.463 0.374 NUE560 9424.3 0.052 1.6E−02 31.56 0.374 6.54NUE560 9422.1 0.058 5.3E−04 46.65 Control 0.040 0.351 NUE562 9252.80.055 4.1E−02 38.43 Control 0.040 NUE564 9243.2 0.049 8.7E−03 38.960.477 2.0E−02 53.10 0.356 2.2E−01 17.03 NUE564 9242.2 0.060 7.6E−0570.29 0.471 3.1E−02 51.25 0.452 8.2E−03 48.52 NUE564 9243.4 0.0458.0E−02 27.01 0.356 4.3E−01 14.34 0.349 2.8E−01 14.73 Control 0.0350.312 0.304 NUE567 9263.2 0.047 1.8E−02 34.46 Control 0.035 NUE5689471.3 0.059 4.2E−05 65.16 0.458 8.3E−02 40.04 0.358 4.4E−01 11.62NUE568 9461.3 0.062 1.5E−03 73.11 0.424 3.6E−01 29.62 0.347 6.9E−01 8.17NUE568 9474.4 0.056 4.1E−03 57.42 0.353 7.6E−01 7.67 0.331 8.6E−01 3.00NUE568 9472.2 0.071 3.5E−07 99.64 0.567 4.6E−03 73.13 0.364 4.0E−0113.42 NUE568 9462.3 0.047 5.2E−02 32.21 0.478 5.4E−02 45.99 0.3693.9E−01 14.82 Control 0.036 0.327 0.321 NUE569 9381.2 0.056 5.3E−0336.52 0.391 9.1E−01 1.25 Control 0.041 0.386 0.353 NUE569 9381.1 0.0538.1E−03 52.48 0.474 6.6E−02 52.21 0.342 4.9E−01 12.36 Control 0.0350.312 0.304 NUE570 9314.1 0.049 1.8E−01 40.41 0.439 2.2E−01 40.98 0.4207.2E−02 38.13 Control 0.041 0.386 0.353 NUE570 9311.4 0.047 3.3E−0232.96 0.530 5.0E−03 70.17 0.456 7.3E−03 49.83 NUE570 9311.3 0.0402.3E−01 15.28 0.426 7.1E−02 36.71 0.397 3.7E−02 30.73 NUE570 9314.40.035 9.4E−01 0.90 0.479 7.4E−03 53.70 0.368 1.1E−01 21.10 NUE570 9314.10.418 7.8E−02 34.04 0.374 1.0E−01 22.95 Control 0.035 0.312 0.304 NUE5719304.2 0.078 3.4E−06 95.35 0.542 1.3E−01 37.40 0.420 2.1E−01 19.40NUE571 9301.1 0.063 9.9E−03 59.31 0.576 2.9E−02 46.03 0.389 4.4E−0110.81 NUE571 9303.2 0.053 1.1E−03 33.00 NUE571 9302.1 0.062 7.2E−0656.48 NUE571 9302.3 0.054 2.1E−02 36.15 0.040 0.394 0.351 NUE571 9304.30.055 6.8E−02 33.34 NUE571 9304.2 0.054 7.6E−02 32.37 NUE571 9303.20.050 1.0E−01 22.33 NUE571 9301.4 0.049 9.7E−02 19.07 Control 0.041NUE572 9324.3 0.049 2.7E−02 24.30 0.410 8.0E−01 3.99 0.381 5.3E−01 8.40Control 0.040 0.394 0.351 NUE573 9491.1 0.053 1.5E−03 49.67 0.4577.9E−02 39.55 0.382 1.9E−01 19.03 NUE573 9491.4 0.050 8.7E−02 40.830.470 8.2E−02 43.44 0.400 1.9E−01 24.64 Control 0.036 0.327 0.321 NUE57410366 0.063 8.1E−01 4.39 0.610 7.5E−03 57.68 0.473 6.5E−04 50.50 Control0.060 0.387 0.315 NUE575 9502.1 0.052 7.9E−03 46.87 0.446 1.1E−01 36.360.343 6.5E−01 6.83 Control 0.036 0.327 0.321 NUE576 9792.4 0.050 2.2E−0116.55 0.504 3.9E−03 44.47 0.390 4.7E−01 8.39 NUE576 9794.1 0.054 5.4E−0226.15 0.357 8.7E−01 2.30 0.392 4.4E−01 9.02 NUE576 9793.3 0.064 1.8E−0351.97 0.386 4.4E−01 10.78 Control 0.043 0.349 0.360 NUE578 9524.3 0.0551.8E−04 54.47 0.374 5.1E−01 14.35 0.360 4.1E−01 12.25 NUE578 9524.10.050 2.5E−02 41.80 Control 0.036 0.327 0.321 NUE579 9701.3 0.0518.7E−02 21.86 0.456 3.9E−03 58.84 0.377 8.8E−02 24.61 Control 0.0420.287 0.302 NUE580 9551.4 0.040 4.3E−01 13.32 0.479 9.3E−02 46.41 0.3614.2E−01 12.46 NUE580 9554.4 0.049 1.2E−02 36.68 0.462 7.3E−02 41.180.382 2.3E−01 18.93 Control 0.036 0.327 0.321 NUE582 9562.4 0.0593.4E−03 40.45 0.591 8.2E−03 32.70 0.414 1.9E−01 12.08 NUE582 9561.20.061 4.1E−03 45.60 Control 0.042 0.445 0.370 NUE583 9671.1 0.0571.3E−01 32.70 0.499 2.2E−02 43.12 0.401 4.5E−01 11.60 Control 0.0430.349 0.360 NUE583 9673.4 0.106 1.1E−05 77.51 0.663 2.0E−04 71.43 0.4164.2E−05 32.10 NUE583 9673.2 0.077 4.3E−02 28.35 0.533 5.6E−02 37.790.331 5.4E−01 5.30 Control 0.060 0.387 0.315 NUE586 9751.7 0.067 5.9E−0349.93 0.548 6.5E−01 6.47 NUE586 9752.1 0.066 4.3E−03 47.72 0.855 4.1E−0566.23 0.563 1.2E−02 32.27 Control 0.044 0.514 0.426 NUE586 9751.1 0.0528.3E−02 24.79 0.379 9.5E−02 32.19 0.393 3.6E−02 30.10 NUE586 9751.70.067 7.5E−03 60.10 0.529 1.7E−03 84.40 0.452 1.5E−03 49.64 NUE5869752.2 0.394 1.4E−02 37.21 0.390 2.6E−02 29.02 NUE586 9752.1 0.0555.8E−02 32.53 0.472 1.9E−02 64.61 0.400 4.5E−02 32.28 Control 0.0420.287 0.302 NUE587 9643.2 0.083 2.5E−06 97.54 0.439 3.8E−03 52.93 0.3147.6E−01 4.01 Control 0.042 0.287 0.302 NUE593 10394 0.074 1.4E−01 33.100.465 9.0E−02 35.46 0.368 6.3E−02 31.07 Control 0.056 0.344 0.281

Table 32: Analyses of plant growth rate (leaf area, root coverage androot length) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogenconditions [normal or regular growth conditions (15 mM N)] as comparedto control plants. “Incr.”=increment; “RGR”=relative growth rate;“Ave.”=average.

Example 6 Assay 2: Nitrogen Use Efficiency: Yield and Plant Growth Rateat Limited and Optimal Nitrogen Concentration Under GreenhouseConditions

This assay follows the seed yield production, biomass formation androsette area growth of plants grown in the greenhouse at nitrogendeficient of nitrogen standard fertilization conditions. Seeds were sownin agar media supplemented with ½ MS medium and a selection agent(Kanamycin). The T₂ transgenic seedlings were then transplanted to 1.7trays filled with peat and perlite. The trays were irrigated with asolution containing constant nitrogen limiting conditions, which wereachieved by irrigating the plants with a solution containing 1.5 mMinorganic nitrogen in the form of KNO₃, supplemented with 1 mM KH₂PO₄, 1mM MgSO₄, 3.6 mM K₂SO₄, 2 mM CaCl₂ and microelements, while normalnitrogen levels were achieved by applying a solution of 6 mM inorganicnitrogen also in the form of KNO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mMCaCl₂ and microelements. All plants were grown in the greenhouse untilmature seeds. Seeds were harvested separately to the above groundtissue, extracted and weight. Plant biomass (the above ground tissue)was also collected and dried for 1 week at 30° C.

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conform by an empty vector carrying the 35Spromoter and the selectable marker were used as controls.

The plants were analyzed for their overall size, growth rate, seedyield, 1,000-seed weight, dry matter and harvest index (HI—seedyield/dry matter). Transgenic plants performance was compared to controlplants grown in parallel under the same conditions.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) was used for capturingimages of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. During the captureprocess, the trays were placed beneath the iron mount, while avoidingdirect sun light and casting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf Growth Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, rosette area, rosette diameter, leaf blade area, plot coverage,leaf petiole length.

Vegetative Growth Rate: is the Rate of Growth of the Plant as Defined byFormulas VIII, IX, X and XIRelative growth rate of leaf blade area=Regression coefficient of leafarea along time course.  Formula VIII:Relative growth rate of rosette area=Regression coefficient of rosettearea along time course.  Formula IX:Relative growth rate of rosette diameter=Regression coefficient ofrosette diameter along time course.  Formula XRelative growth rate of plot coverage=Regression coefficient of plotcoverage along time course.  Formula XI

Seeds Average Weight (Seed Weight or 1000 Seed Weight)—

At the end of the experiment all seeds were collected. The seeds werescattered on a glass tray and a picture was taken. Using the digitalanalysis, the number of seeds in each sample was calculated.

Plant Dry Weight and Seed Yield—

On about day 80 from sowing, the plants were harvested and left to dryat 30° C. in a drying chamber. The biomass and seed weight of each plotwere measured and divided by the number of plants in each plot.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 30° C. in a drying chamber; Seed yieldper plant=total seed weight per plant (grams).

The Harvest Index can be calculated using Formula III (as describedabove; Harvest Index=Average seed yield per plant/Average dry weight).

Statistical Analyses—

To identify genes conferring significantly improved nitrogen useefficiency and yield production, the results obtained from thetransgenic plants were compared to those obtained from control plants.To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Datawas analyzed using Student's t-test and results were consideredsignificant if the p value was less than 0.1. The JMP statisticssoftware package was used (Version 5.2.1, SAS Institute Inc., Cary,N.C., USA).

Experiment Results:

The genes presented in Tables 33, 34 and 35, hereinbelow, have improvedplant NUE when grown at limiting nitrogen concentration levels. Thesegenes produced higher seed yield, harvest index, seed weight (expressedas 1000-seed weight) and plant biomass [(as expressed as plant dryweight (DW)] when grown under limiting nitrogen growth conditions,compared to control.

Tables 33, 34 and 35 depict analyses of seed yield, harvest index, seedsize (expressed as 1000-seed weight) when grown under limiting nitrogenconditions in plants overexpressing the polynucleotides of someembodiments of the invention under the regulation of a constitutive(35S). Evaluation of each gene was performed by testing the performanceof several events. Some of the genes were evaluated in more than onetissue culture assay and the results obtained were repeated. Event withp-value<0.1 was considered statistically significant.

TABLE 33 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved seed yield and weight(expressed as 1000-seed weight) under nitrogen deficient growthconditions. Gene Seed Yield Gene Seeds Weight Name Event # Ave. p-value% incr. Name Event # Ave. p-value % incr. NUE241 9631.6 0.169 1.3E−0112.60 NUE241 9632.3 0.020 2.5E−01 2.46 NUE241 9631.4 0.150 9.7E−01 0.40NUE241 9631.4 0.020 6.4E−01 1.96 Control 0.150 Control 0.020 NUE2488982.3 0.144 3.6E−01 7.27 NUE248 8982.4 0.023 4.1E−03 14.08 Control0.135 NUE248 8982.3 0.021 4.3E−01 4.59 NUE525 9534.1 0.161 6.7E−01 7.33NUE248 8981.1 0.021 7.2E−01 7.54 NUE525 9531.3 0.169 5.6E−01 12.83NUE248 8983.1 0.021 7.3E−01 5.14 NUE525 9533.4 0.162 7.0E−01 8.26Control 0.020 NUE525 9531.1 0.166 1.0E−01 10.90 NUE255 9431.4 0.0211.0E−01 4.57 Control 0.150 Control 0.020 NUE536 9234.1 0.157 6.1E−0116.81 NUE525 9533.1 0.022 2.1E−01 10.94 Control 0.135 NUE525 9531.30.020 7.6E−01 2.55 NUE545 9482.4 0.184 1.7E−04 22.72 Control 0.020Control 0.150 NUE536 9234.1 0.020 6.0E−01 3.02 NUE565 9443.4 0.2041.8E−01 36.33 NUE536 9231.3 0.021 5.3E−01 4.99 Control 0.150 Control0.020 NUE566 9514.3 0.163 1.6E−01 9.08 NUE545 9482.4 0.020 7.1E−01 1.36NUE566 9514.1 0.172 7.0E−01 15.02 Control 0.020 Control 0.150 NUE5499343.6 0.023 2.7E−01 14.91 NUE568 9471.3 0.160 1.8E−01 6.55 NUE5499342.3 0.021 5.1E−01 3.66 Control 0.150 Control 0.020 NUE573 9493.40.172 3.8E−01 14.54 NUE560 9424.1 0.023 1.3E−04 18.35 NUE573 9491.20.181 3.3E−04 20.87 NUE560 9424.3 0.021 8.0E−02 4.76 NUE573 9492.2 0.1558.9E−01 3.21 NUE560 9422.1 0.020 3.1E−01 3.38 Control 0.150 Control0.020 NUE578 9524.1 0.147 9.3E−01 −1.70 NUE568 9461.2 0.024 1.3E−0521.77 Control 0.150 Control 0.020 NUE580 9552.3 0.180 1.9E−01 19.99NUE573 9491.2 0.023 1.1E−01 14.40 Control 0.150 NUE573 9492.2 0.0213.6E−02 5.14 NUE585 9661.1 0.150 1.8E−01 11.29 Control 0.020 Control0.135 NUE578 9524.1 0.022 8.2E−04 10.87 Control 0.020 NUE580 9551.30.025 7.2E−02 24.52 NUE580 9554.4 0.023 9.7E−02 14.78 Control 0.020NUE585 9662.4 0.021 7.5E−02 6.26 NUE585 9661.1 0.022 5.2E−03 9.36Control 0.020

Table 33: Analyses of seed yield and weight of transgenic plantsoverexpressing the exogenous polynucleotides of some embodiments of theinvention (using the cloned or synthetic genes listed in Table 23 above)under the regulation of a constitutive promoter (35S) when grown undernitrogen deficient conditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6mM K₂SO₄, 2 mM CaCl₂ and microelements) as compared to control plants.“Incr.”=increment; “Ave.”=average.

TABLE 34 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved harvest index undernitrogen deficient growth conditions. Harvest Index Gene Name Event #Average p-value % increment NUE525 9534.1 0.321 1.9E−01 6.99 NUE5259533.1 0.319 7.0E−01 6.25 NUE525 9533.4 0.322 4.8E−01 7.18 NUE525 9531.10.356 3.5E−02 18.72 Control 0.300 NUE536 9234.1 0.344 6.9E−02 20.95NUE536 9231.3 0.298 3.7E−01 4.68 Control 0.285 NUE545 9482.4 0.3282.0E−01 9.31 Control 0.300 NUE549 9341.1 0.337 2.9E−02 12.21 NUE5499342.3 0.322 6.8E−01 7.19 Control 0.300 NUE560 9424.3 0.316 9.5E−0210.86 NUE560 9422.1 0.318 8.2E−02 11.60 Control 0.285 NUE565 9443.40.335 3.9E−01 11.59 Control 0.300 NUE566 9514.1 0.351 5.8E−01 17.05Control 0.300

Table 34: Analyses of harvest index of transgenic plants overexpressingthe exogenous polynucleotides of some embodiments of the invention(using the cloned or synthetic genes listed in Table 23 above) under theregulation of a constitutive promoter (35S) when grown under nitrogendeficient conditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mMK₂SO₄, 2 mM CaCl₂ and microelements) as compared to control plants.

TABLE 35 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved dry weight undernitrogen deficient growth conditions Dry Weight Gene Name Event #Average p-value % increment NUE241 9631.6 0.569 5.2E−01 11.66 NUE2419632.3 0.613 8.0E−02 20.25 Control 0.509 NUE248 8982.3 0.534 2.9E−0113.35 Control 0.471 NUE525 9531.3 0.600 4.3E−01 17.79 Control 0.509NUE545 9482.4 0.561 2.9E−01 10.18 Control 0.509 NUE549 9342.2 0.5417.3E−01 6.13 Control 0.509 NUE565 9444.1 0.589 6.5E−01 15.71 NUE5659443.4 0.609 3.4E−02 19.63 Control 0.509 NUE566 9514.3 0.637 5.2E−0124.96 Control 0.509 NUE568 9471.3 0.515 8.9E−01 1.10 NUE568 9462.3 0.6391.4E−02 25.52 Control 0.509 NUE573 9493.4 0.581 2.9E−01 14.15 NUE5739491.2 0.613 3.4E−02 20.37 NUE573 9492.2 0.683 4.2E−02 33.99 Control0.509 NUE580 9552.3 0.561 10.18 NUE580 9551.3 0.634 2.4E−01 24.42 NUE5809554.4 0.526 7.0E−01 3.31 Control 0.509 NUE585 9661.1 0.519 4.6E−0110.30 Control 0.471

Table 35: Analyses of dry weight of transgenic plants overexpressing theexogenous polynucleotides of some embodiments of the invention (usingthe cloned or synthetic genes listed in Table 23 above) under theregulation of a constitutive promoter (35S) when grown under nitrogendeficient conditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mMK₂SO₄, 2 mM CaCl₂ and microelements) as compared to control plants.

The genes presented in Tables 36 and 37, hereinbelow, have improvedplant NUE since they produced higher seed yield, harvest index, seedweight (expressed as 1000-seed weight) and plant biomass [(as expressedas plant dry weight (DW)] when grown under standard nitrogen growthconditions, compared to control plants indicating the high ability ofthe plant to better metabolize the nitrogen present in the medium.

Tables 36 and 37 depict analyses of dry weight, seed yield, harvestindex, seed size (expressed as 1000-seed weight) when grown understandard nitrogen conditions (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mMCaCl₂ and microelements) in plants overexpressing the polynucleotides ofsome embodiments of the invention under the regulation of a constitutivepromoter (35S). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 36 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (dryweight) and seed yield under standard nitrogen conditions Gene DryWeight Gene Seed Yield Name Event # Ave. P-Value % incr. Name Event #Ave. P-Value % incr. NUE255 9431.4 1.394 5.6E−01 14.344 NUE234 9162.50.523 1.0E−01 16.207 NUE255 9432.1 1.499 5.9E−02 22.894 Control 0.450NUE255 9433.1 1.409 1.0E−01 15.530 NUE241 9631.4 0.364 3.0E−01 −12.185Control 1.219 Control 0.414 NUE525 9531.1 1.635 6.1E−03 34.085 NUE2559431.4 0.436 7.4E−01 5.346 Control 1.219 NUE255 9432.1 0.483 3.5E−0116.593 NUE545 9484.2 1.246 7.0E−01 2.204 NUE255 9433.1 0.444 1.0E−017.100 NUE545 9481.3 1.631 3.4E−04 33.726 Control 0.414 Control 1.219NUE525 9534.1 0.486 2.6E−03 17.328 NUE549 9341.1 1.381 6.7E−02 13.275NUE525 9531.1 0.504 4.0E−01 21.719 NUE549 9342.3 1.310 2.7E−01 7.432Control 0.414 Control 1.219 NUE549 9341.1 0.447 6.4E−01 8.031 NUE5639451.1 1.303 5.6E−01 6.868 Control 0.414 NUE563 9452.3 1.473 1.6E−0220.788 NUE563 9451.1 0.419 8.6E−01 1.237 Control 1.219 NUE563 9452.30.454 6.5E−01 9.572 NUE565 9443.2 1.348 9.3E−02 10.507 Control 0.414NUE565 9444.3 1.376 4.7E−01 12.814 NUE566 9512.4 0.458 1.8E−01 10.657Control 1.219 Control 0.414 NUE566 9512.4 1.471 5.8E−03 20.605 NUE5689464.2 0.429 8.6E−01 3.674 Control 1.219 NUE568 9462.3 0.423 7.7E−012.233 NUE568 9461.2 1.571 8.6E−04 28.806 Control 0.414 NUE568 9464.21.366 4.9E−01 11.994 NUE573 9491.4 0.455 5.1E−01 9.769 NUE568 9462.31.288 3.8E−01 5.638 NUE573 9492.1 0.486 4.5E−01 17.465 Control 1.219NUE573 9493.4 0.449 7.5E−01 8.482 NUE573 9491.4 1.249 8.9E−01 2.460Control 0.414 NUE573 9492.1 1.668 1.3E−01 36.802 NUE582 9561.2 0.4524.7E−01 9.239 NUE573 9493.4 1.478 7.2E−02 21.213 Control 0.414 NUE5739491.2 1.407 1.0E−01 15.377 Control 1.219

Table 36: Analyses of plant biomass (dry weight) and see yield oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under standard nitrogen conditions (6 mM KNO₃, 1 mM KH₂PO₄, 1mM MgSO₄, 2 mM CaCl₂ and microelements) as compared to control plants.“Incr.”=increment; “RGR”=relative growth rate; “Ave.”=average.

TABLE 37 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved harvest index andseed weight under standard nitrogen conditions Gene Harvest Index GeneSeed Weight Name Event # Ave. P-value % incr. Name Event # Ave. P-Value% incr. NUE234 9162.5 0.368 3.7E−02 15.734 NUE241 9631.4 0.022 3.3E−026.274 Control 0.318 Control 0.021 NUE525 9534.1 0.477 4.5E−01 38.546NUE255 9432.1 0.023 2.5E−02 11.793 Control 0.344 NUE255 9433.1 0.0216.6E−02 4.087 NUE573 9491.4 0.366 3.7E−01 6.179 Control 0.021 Control0.344 NUE525 9534.1 0.021 1.0E+00 0.008 NUE582 9561.2 0.466 2.2E−0135.384 NUE525 9531.1 0.025 3.6E−01 22.277 Control 0.344 Control 0.021NUE545 9481.3 0.024 3.0E−01 17.664 Control 0.021 NUE549 9341.1 0.0225.8E−01 9.152 NUE549 9342.3 0.023 5.6E−01 11.416 Control 0.021 NUE5639451.1 0.023 1.1E−01 10.668 NUE563 9452.3 0.022 3.5E−01 5.095 Control0.021 NUE565 9443.2 0.024 2.0E−01 15.540 NUE565 9444.3 0.021 9.1E−011.159 Control 0.021 NUE566 9512.4 0.022 8.6E−02 5.164 Control 0.021NUE568 9461.2 0.024 3.0E−01 19.048 NUE568 9464.2 0.022 6.1E−01 8.243NUE568 9462.3 0.023 6.1E−04 10.961 Control 0.021 NUE573 9491.4 0.0217.1E−01 1.229 NUE573 9492.1 0.021 1.8E−01 3.164 NUE573 9493.4 0.0221.9E−01 8.883 NUE573 9491.2 0.023 4.0E−01 14.335 Control 0.021 NUE5829561.2 0.024 1.6E−03 15.172 Control 0.021

Table 37: Analyses of harvest index and seed weight of transgenic plantsoverexpressing the exogenous polynucleotides of some embodiments of theinvention (using the cloned or synthetic genes listed in Table 23 above)under the regulation of a constitutive promoter (35S) when grown understandard nitrogen conditions (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mMCaCl₂ and microelements) as compared to control plants.“Incr.”=increment; “RGR”=relative growth rate; “Ave.”=average.

Improvement of rosette area as well as rosette growth rate supports thefact that plants can produce larger plant biomass by better exploitingthe nitrogen available in the soil. In addition a production of a largernumber of leaves as well as a higher plot coverage when grown at lownitrogen conditions indicate a larger photosynthetic capacity of theplant when grown at different nitrogen growth conditions

The genes presented in Tables 38 and 39, hereinbelow, have improvedplant NUE and produced larger plant biomass when grown under limitingnitrogen growth conditions, compared to control plants. In addition aproduction of a larger number of leaves as well as a higher plotcoverage when grown at low nitrogen conditions indicate a largerphotosynthetic capacity of the plant when grown at low nitrogen growthconditions

Tables 38 and 39 depict analyses of rosette area and leaf number(rosette diameter, rosette area, leaf number, leaf blade area and plotcoverage) when grown under limiting nitrogen conditions (1.5 mM KNO₃, 1mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM K₂SO₄, 2 mM CaCl₂ and microelements) inplants overexpressing the polynucleotides of some embodiments of theinvention under the regulation of a constitutive promoter (35S).Evaluation of each gene was performed by testing the performance ofseveral events. Some of the genes were evaluated in more than one tissueculture assay and the results obtained were repeated. Event withp-value<0.1 was considered statistically significant.

TABLE 38 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved rosette growthperformance (rosette diameter and area and plot coverage) under nitrogendeficient conditions Gene Rosette Diameter [cm] Rosette Area [cm²] PlotCoverage [%] Name Event # Ave. p-value % incr. Ave. p-value % incr. Ave.p-value % incr. NUE234 9163.4 2.26 1.6E−01 8.49 1.634 2.7E−01 7.24 13.072.7E−01 7.24 Control 2.08 1.523 12.19 NUE241 9632.3 1.72 4.1E−01 20.771.008 5.3E−01 42.18 8.06 5.3E−01 42.18 NUE241 9631.4 1.57 6.4E−02 10.200.886 1.7E−03 24.97 7.09 1.7E−03 24.97 Control 1.42 0.709 5.67 NUE2499122.2 2.20 2.3E−01 5.86 1.696 2.6E−01 11.33 13.57 2.6E−01 11.33 Control2.08 1.523 12.19 NUE525 9534.1 1.84 9.3E−02 29.75 1.255 6.6E−02 77.069.45 1.9E−01 66.71 NUE525 9531.2 1.83 2.1E−02 29.02 1.191 8.3E−02 68.098.98 2.2E−01 58.33 NUE525 9533.1 1.74 7.1E−02 22.38 1.060 1.4E−01 49.548.48 1.4E−01 49.54 NUE525 9531.3 1.58 4.3E−01 11.39 0.884 5.3E−01 24.707.07 5.3E−01 24.70 NUE525 9533.4 1.71 1.5E−03 20.13 1.048 1.5E−04 47.848.38 1.5E−04 47.84 NUE525 9531.1 1.62 8.0E−02 13.70 1.025 2.8E−02 44.648.20 2.8E−02 44.64 Control 1.42 0.709 5.67 NUE536 9234.1 2.29 2.1E−029.74 1.734 3.2E−01 13.79 13.87 3.2E−01 13.79 Control 2.08 1.523 12.19NUE545 9484.2 1.97 1.5E−07 38.83 1.311 4.1E−08 84.91 10.49 4.1E−08 84.91NUE545 9482.4 1.71 2.1E−01 19.99 0.999 2.1E−01 40.87 7.99 2.1E−01 40.87NUE545 9481.3 1.79 4.6E−01 26.22 1.025 4.9E−01 44.66 8.20 4.9E−01 44.66NUE545 9484.4 2.01 1.6E−07 41.45 1.265 2.1E−05 78.42 10.12 2.1E−05 78.42Control 1.42 0.709 5.67 NUE549 9341.1 1.61 13.18 0.956 2.2E−01 34.917.65 2.2E−01 34.91 Control 1.42 0.709 5.67 NUE563 9454.1 1.61 1.6E−0113.12 0.930 1.0E−01 31.25 7.44 1.0E−01 31.25 NUE563 9452.3 1.56 4.6E−0110.02 0.828 5.7E−01 16.76 6.62 5.7E−01 16.76 NUE563 9453.4 1.72 1.7E−0121.08 1.077 1.9E−01 51.96 8.62 1.9E−01 51.96 NUE563 9452.1 1.48 7.5E−024.31 0.720 8.4E−01 1.64 5.76 8.4E−01 1.64 Control 1.42 0.709 5.67 NUE5659444.1 1.72 3.1E−01 20.73 0.889 2.7E−01 25.43 7.11 2.7E−01 25.43 NUE5659442.4 1.63 8.5E−04 14.54 0.839 9.5E−02 18.42 6.72 9.5E−02 18.42 Control1.42 0.709 5.67 NUE566 9514.3 1.75 2.1E−01 22.85 1.113 2.3E−01 56.978.27 1.2E−01 45.76 NUE566 9513.1 1.63 3.0E−01 14.74 0.915 2.3E−01 29.047.32 2.3E−01 29.04 NUE566 9512.4 1.58 5.0E−01 11.26 0.927 4.3E−01 30.797.42 4.3E−01 30.79 NUE566 9514.1 1.72 6.9E−02 21.08 1.061 2.3E−01 49.668.02 3.7E−01 41.52 Control 1.42 0.709 5.67 NUE568 9474.4 1.66 2.3E−0116.75 0.937 2.9E−02 32.20 7.50 2.9E−02 32.20 NUE568 9461.2 1.79 2.1E−0126.22 1.216 1.6E−01 71.50 9.72 1.6E−01 71.50 NUE568 9462.4 1.76 3.3E−0123.46 1.072 2.7E−01 51.17 8.57 2.7E−01 51.17 NUE568 9462.3 1.69 5.8E−0219.20 1.005 1.8E−01 41.72 8.04 1.8E−01 41.72 NUE568 9463.4 1.78 2.3E−0125.46 1.018 3.6E−01 43.57 8.14 3.6E−01 43.57 NUE568 9473.3 1.52 3.4E−016.95 0.826 1.9E−01 16.55 6.61 1.9E−01 16.55 Control 1.42 0.709 5.67NUE573 9491.4 1.72 2.1E−01 21.02 1.007 2.4E−01 42.03 8.05 2.4E−01 42.03NUE573 9492.1 2.01 1.1E−05 41.63 1.404 4.1E−03 98.05 11.23 4.1E−03 98.05NUE573 9493.4 1.77 4.9E−06 24.30 1.106 3.3E−04 56.00 8.31 1.5E−01 46.46NUE573 9494.3 1.82 1.4E−06 27.69 1.177 6.2E−03 66.10 9.42 6.2E−03 66.10NUE573 9491.2 1.79 1.2E−01 26.17 1.115 1.8E−01 57.32 8.92 1.8E−01 57.32NUE573 9492.2 1.75 1.2E−01 22.83 1.016 1.5E−01 43.40 8.13 1.5E−01 43.40Control 1.42 0.709 5.67 NUE575 9501.4 2.04 1.4E−02 43.55 1.338 8.7E−0888.73 10.70 8.7E−08 88.73 NUE575 9504.1 1.93 1.8E−01 35.73 1.259 2.5E−0177.60 10.07 2.5E−01 77.60 NUE575 9503.1 1.84 2.2E−01 29.22 1.282 2.0E−0180.88 10.26 2.0E−01 80.88 NUE575 9502.1 1.73 2.7E−01 21.38 1.097 2.1E−0154.82 8.78 2.1E−01 54.82 Control 1.42 0.709 5.67 NUE578 9524.3 1.926.1E−02 34.78 1.274 4.5E−02 79.68 10.19 4.5E−02 79.68 NUE578 9524.1 2.131.5E−01 49.88 1.602 1.4E−01 126.00 12.12 2.2E−01 #### NUE578 9523.3 1.971.9E−02 38.35 1.400 4.0E−02 97.45 11.20 4.0E−02 97.45 NUE578 9522.3 1.754.8E−04 22.83 1.095 6.4E−05 54.54 8.76 6.4E−05 54.54 Control 1.42 0.7095.67 NUE580 9552.3 1.52 1.2E−01 6.68 0.783 3.5E−02 10.46 6.26 3.5E−0210.46 NUE580 9551.3 1.71 1.7E−01 19.93 1.049 2.0E−01 48.02 8.39 2.0E−0148.02 NUE580 9553.4 1.73 1.1E−05 21.63 1.058 5.1E−06 49.24 8.46 5.1E−0649.24 NUE580 9551.4 1.85 6.7E−02 30.17 1.284 6.8E−02 81.21 10.28 6.8E−0281.21 NUE580 9554.4 1.70 2.7E−01 19.55 1.084 2.8E−01 52.96 8.67 2.8E−0152.96 Control 1.42 0.709 5.67 NUE582 9561.1 1.73 3.0E−01 21.81 1.0263.3E−01 44.69 7.60 2.5E−01 34.04 NUE582 9562.1 1.60 3.4E−01 12.36 0.9852.2E−01 38.99 7.88 2.2E−01 38.99 NUE582 9562.4 1.58 4.7E−01 11.39 0.9204.7E−01 29.79 7.00 6.1E−01 23.36 NUE582 9563.3 1.76 2.1E−01 23.73 1.0711.4E−01 51.05 8.57 1.4E−01 51.05 NUE582 9561.2 1.92 6.2E−02 34.91 1.3289.8E−02 87.34 10.02 2.1E−01 76.63 Control 1.42 0.709 5.67

Table 38: Analyses of rosette diameter and area and plot coverage oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under nitrogen deficient conditions (1.5 mM KNO₃, 1 mMKH₂PO₄, 1 mM MgSO₄, 3.6 mM K₂SO₄, 2 mM CaCl₂ and microelements) ascompared to control plants. “Incr.”=increment; “Ave.”=average.

TABLE 39 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved rosette growthperformance (leaf number and leaf blade) under nitrogen deficientconditions Gene Leaf Number Leaf Blade Area [cm²] Name Event # Ave.p-value % incr. Ave. p-value % incr. NUE241 9632.3 8.0 6.4E−01 7.340.174 5.3E−01 30.98 NUE241 9631.4 8.3 1.6E−01 10.69 0.156 2.8E−02 17.43Control 7.5 0.133 NUE249 9122.2 9.8 1.1E−01 4.20 0.266 4.7E−02 12.23Control 9.4 0.237 NUE525 9534.1 8.8 5.9E−02 17.88 0.199 1.6E−01 49.90NUE525 9531.2 8.9 3.6E−05 19.80 0.181 4.1E−02 36.68 NUE525 9533.1 7.81.9E−02 4.82 0.175 3.3E−01 31.80 NUE525 9531.3 7.6 8.6E−01 2.31 0.1496.4E−01 12.26 NUE525 9533.4 8.3 8.0E−02 11.53 0.176 2.0E−02 32.40 NUE5259531.1 8.4 3.0E−05 12.37 0.161 3.4E−03 21.39 Control 7.5 0.133 NUE5369234.1 9.4 9.7E−01 0.19 0.266 3.0E−01 12.00 Control 9.4 0.237 NUE5459484.2 8.7 4.6E−02 16.56 0.216 2.8E−03 62.69 NUE545 9482.4 8.0 4.7E−017.34 0.174 1.6E−01 31.20 NUE545 9481.3 7.8 7.2E−01 3.98 0.188 4.7E−0141.85 NUE545 9484.4 8.3 1.6E−01 10.69 0.207 2.6E−07 56.09 Control 7.50.133 NUE549 9341.1 7.9 6.7E−01 5.66 0.160 8.3E−02 20.34 Control 7.50.133 NUE563 9454.1 8.3 1.6E−01 10.69 0.154 1.7E−01 16.19 NUE563 9452.37.4 9.8E−01 −0.21 0.150 6.1E−01 13.28 NUE563 9453.4 8.1 1.9E−01 9.010.181 2.2E−01 36.86 NUE563 9452.1 0.141 4.4E−01 6.33 Control 7.5 0.133NUE565 9444.1 7.7 8.6E−02 3.14 0.162 2.2E−01 21.85 NUE565 9442.4 7.81.9E−02 4.82 0.148 1.4E−01 11.60 Control 7.5 0.133 NUE566 9514.3 7.84.5E−01 4.94 0.188 5.6E−02 41.70 NUE566 9513.1 7.8 1.8E−01 3.98 0.1623.2E−01 22.40 NUE566 9512.4 8.1 5.4E−01 8.18 0.170 3.9E−01 28.31 NUE5669514.1 8.0 4.2E−01 7.82 0.180 1.9E−01 35.77 Control 7.5 0.133 NUE5689474.4 7.9 3.1E−01 5.66 0.175 4.2E−02 31.80 NUE568 9461.2 8.6 9.9E−0315.72 0.195 9.6E−02 47.20 NUE568 9462.4 8.1 1.3E−01 8.18 0.193 3.2E−0145.51 NUE568 9462.3 7.8 5.7E−01 3.98 0.176 5.3E−02 32.52 NUE568 9463.47.6 6.1E−01 2.31 0.185 3.7E−01 39.91 NUE568 9473.3 7.9 4.6E−01 6.500.148 1.8E−01 11.71 Control 7.5 0.133 NUE573 9491.4 7.9 6.7E−01 5.660.168 2.6E−01 27.07 NUE573 9492.1 9.1 6.1E−02 22.43 0.234 3.8E−02 76.54NUE573 9493.4 8.0 3.1E−01 7.94 0.193 9.4E−07 45.94 NUE573 9494.3 8.13.6E−02 9.01 0.193 1.3E−05 45.95 NUE573 9491.2 8.7 2.0E−01 16.56 0.1812.2E−01 36.33 NUE573 9492.2 7.6 6.7E−01 1.47 0.183 5.8E−02 38.25 Control7.5 0.133 NUE575 9501.4 8.5 1.1E−01 14.05 0.216 1.9E−02 62.82 NUE5759504.1 8.5 2.0E−01 14.05 0.214 2.2E−01 61.54 NUE575 9503.1 8.4 3.8E−0113.21 0.207 2.1E−01 55.92 NUE575 9502.1 8.4 2.5E−01 13.21 0.182 2.6E−0137.35 Control 7.5 0.133 NUE578 9524.3 8.4 2.3E−01 12.37 0.208 1.5E−0757.07 NUE578 9524.1 9.1 1.3E−01 22.19 0.242 1.6E−01 82.58 NUE578 9523.38.8 3.1E−06 17.40 0.223 7.2E−02 68.19 NUE578 9522.3 8.4 2.3E−01 12.370.178 2.4E−04 34.51 Control 7.5 0.133 NUE580 9552.3 8.1 1.6E−03 8.180.135 6.4E−01 1.85 NUE580 9551.3 8.5 1.3E−05 14.05 0.175 2.4E−01 31.72NUE580 9553.4 7.9 1.8E−01 6.50 0.185 1.4E−01 39.73 NUE580 9551.4 8.52.0E−01 14.05 0.202 2.0E−05 52.26 NUE580 9554.4 7.9 9.4E−02 5.66 0.1833.4E−01 38.26 Control 7.5 0.133 NUE582 9561.1 8.3 2.8E−01 11.29 0.1713.9E−01 28.94 NUE582 9562.1 8.1 1.9E−01 9.01 0.168 3.4E−01 26.84 NUE5829562.4 7.7 5.3E−01 2.67 0.164 5.0E−01 24.06 NUE582 9563.3 8.4 1.0E−0413.21 0.186 1.5E−01 40.09 NUE582 9561.2 8.7 7.5E−02 16.08 0.217 1.5E−0163.53 Control 7.5 0.133

Table 39: Analyses of leaf number and leaf blade of transgenic plantsoverexpressing the exogenous polynucleotides of some embodiments of theinvention (using the cloned or synthetic genes listed in Table 23 above)under the regulation of a constitutive promoter (35S) when grown undernitrogen deficient conditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6mM K₂SO₄, 2 mM CaCl₂ and microelements) as compared to control plants.“Incr.”=increment; “Ave.”=average.

The genes presented in Tables 40 and 41, hereinbelow, have improvedplant growth rate when grown at limiting nitrogen fertilization levels.These genes improved the growth rate of the rosette and faster coveredthe soil when grown at limiting nitrogen growth conditions.

Tables 40 and 41 depict analyses of the growth rate of the rosettediameter, rosette area, leaf blade area, leaf number and plot coveragewhen grown under limiting nitrogen conditions (1.5 mM KNO₃, 1 mM KH₂PO₄,1 mM MgSO₄, 3.6 mM K₂SO₄, 2 mM CaCl₂ and microelements) in plantsoverexpressing the polynucleotides of some embodiments of the inventionunder the regulation of a constitutive promoter (35S). Evaluation ofeach gene was performed by testing the performance of several events.Some of the genes were evaluated in more than one tissue culture assayand the results obtained were repeated. Event with p-value<0.1 wasconsidered statistically significant.

TABLE 40 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved growth rate (RGR ofleaf blade area and RGR of leaf number) under nitrogen deficientconditions Gene RGR Of Leaf Blade Area RGR Of Leaf Number Name Event #Average p-value % increment Average p-value % increment NUE241 9633.40.016 8.8E−01 1.45 0.567 0.330 13.34 NUE241 9632.3 0.021 4.2E−02 30.380.548 0.538 9.72 NUE241 9631.4 0.019 6.1E−02 17.16 0.536 0.579 7.27Control 0.016 0.500 NUE525 9534.1 0.023 6.7E−05 43.97 0.612 0.105 22.43NUE525 9531.2 0.020 6.2E−03 25.68 0.661 0.016 32.14 NUE525 9533.1 0.0202.0E−02 25.03 0.503 0.954 0.71 NUE525 9531.3 0.017 7.1E−01 3.98 0.5080.918 1.54 NUE525 9533.4 0.020 1.4E−02 22.12 0.560 0.372 11.94 NUE5259531.1 0.018 1.3E−01 13.01 0.557 0.392 11.36 Control 0.016 0.500 NUE5459484.2 0.026 1.1E−07 60.54 NUE545 9482.4 0.021 6.0E−03 28.52 NUE5459482.2 0.018 9.81 NUE545 9481.3 0.022 2.1E−02 39.32 NUE545 9484.4 0.0251.3E−06 54.25 Control 0.016 NUE549 9341.1 0.018 1.6E−01 12.49 Control0.016 NUE563 9454.1 0.018 1.4E−01 13.13 0.544 0.509 8.79 NUE563 9452.30.018 3.7E−01 9.76 NUE563 9453.4 0.021 2.7E−03 30.97 0.580 0.223 16.07Control 0.016 0.500 NUE565 9444.1 0.020 3.2E−02 21.06 Control 0.016NUE566 9514.3 0.022 3.5E−03 34.09 0.558 0.384 11.55 NUE566 9513.1 0.0203.2E−02 22.23 0.551 0.432 10.19 NUE566 9512.4 0.021 1.2E−02 31.16 0.5750.285 14.98 NUE566 9514.1 0.021 5.5E−03 29.97 0.515 3.05 Control 0.0160.500 NUE568 9474.4 0.022 6.2E−04 34.41 NUE568 9461.2 0.024 1.2E−0446.02 0.567 0.327 13.35 NUE568 9462.4 0.024 5.3E−04 48.85 0.527 0.6875.52 NUE568 9462.3 0.022 1.2E−03 35.77 NUE568 9463.4 0.022 2.5E−03 38.12Control 0.016 0.500 NUE573 9491.4 0.018 1.4E−01 14.16 NUE573 9492.10.029 7.4E−09 77.15 0.606 0.122 21.29 NUE573 9493.4 0.023 9.0E−05 42.330.539 0.549 7.79 NUE573 9494.3 0.023 1.3E−04 40.79 0.573 0.271 14.63NUE573 9491.2 0.020 9.4E−03 26.53 0.565 0.383 13.00 NUE573 9492.2 0.0224.2E−04 39.19 Control 0.016 0.500 NUE575 9501.4 0.026 2.8E−07 59.000.554 0.441 10.78 NUE575 9504.3 0.016 8.9E−01 −1.86 0.517 0.813 3.41NUE575 9504.1 0.025 1.3E−04 55.64 0.560 0.398 12.06 NUE575 9503.1 0.0241.4E−04 51.12 0.615 0.126 23.04 NUE575 9502.1 0.021 1.5E−02 28.36 0.5130.852 2.58 Control 0.016 0.500 NUE578 9524.3 0.025 4.9E−06 56.63 0.5750.268 14.98 NUE578 9524.1 0.029 3.0E−07 77.86 0.630 0.050 25.93 NUE5789523.3 0.027 6.9E−08 65.64 0.561 0.372 12.29 NUE578 9522.3 0.021 1.6E−0332.66 0.606 0.119 21.29 Control 0.016 0.500 NUE580 9551.3 0.021 6.5E−0328.15 0.538 0.545 7.62 NUE580 9554.2 0.564 0.339 12.76 NUE580 9553.40.023 1.5E−04 40.79 0.526 0.686 5.28 NUE580 9551.4 0.024 1.3E−05 47.330.524 0.734 4.82 NUE580 9554.4 0.022 4.0E−03 37.25 Control 0.016 0.500NUE582 9561.1 0.021 1.3E−02 29.36 0.585 0.225 16.94 NUE582 9562.1 0.0201.8E−02 26.50 0.560 0.379 11.94 NUE582 9562.4 0.020 3.7E−02 26.29 0.5560.464 11.24 NUE582 9563.3 0.023 1.7E−04 44.35 0.615 0.113 23.04 NUE5829561.2 0.026 1.4E−06 61.66 0.605 0.124 21.11 Control 0.016 0.500

Table 40: Analyses of growth rate (RGR of leaf blade area and RGR ofleaf number) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under nitrogen deficientconditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM K₂SO₄, 2 mMCaCl₂ and microelements) as compared to control plants.

TABLE 41 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved growth rate (RGR ofrosette area and diameter and RGR of plot coverage) under nitrogendeficient conditions Gene RGR Of Rosette Area RGR Of Rosette DiameterRGR Of Plot Coverage Name Event # Ave. p-value % incr. Ave. p-value %incr. Ave. p-value % incr. NUE241 9633.4 0.102 2.9E−01 13.04 0.1306.4E−01 −3.23 0.82 2.9E−01 13.04 NUE241 9632.3 0.131 2.2E−02 44.18 0.1635.3E−02 21.41 1.05 2.2E−02 44.18 NUE241 9631.4 0.114 2.6E−02 25.68 0.1518.4E−02 12.12 0.91 2.6E−02 25.68 Control 0.091 0.135 0.72 NUE525 9534.10.160 3.4E−07 76.36 0.164 3.4E−03 21.78 1.20 2.3E−05 66.06 NUE525 9531.20.150 3.9E−06 65.52 0.161 7.7E−03 19.52 1.13 2.0E−04 55.87 NUE525 9533.10.135 3.4E−04 48.53 0.153 5.1E−02 13.37 1.08 3.4E−04 48.53 NUE525 9531.30.111 1.1E−01 22.62 0.135 9.8E−01 0.21 0.89 1.1E−01 22.62 NUE525 9533.40.130 3.6E−04 43.83 0.149 1.1E−01 11.07 1.04 3.6E−04 43.83 NUE525 9531.10.129 6.5E−04 42.14 0.140 5.5E−01 4.10 1.03 6.5E−04 42.14 Control 0.0910.135 0.72 NUE545 9484.2 0.168 4.4E−08 85.85 0.184 4.2E−06 36.37 1.354.4E−08 85.85 NUE545 9482.4 0.127 3.1E−03 40.34 0.154 6.9E−02 14.35 1.023.1E−03 40.34 NUE545 9482.2 0.098 8.66 0.135 9.6E−01 0.34 0.79 4.4E−018.66 NUE545 9481.3 0.130 2.3E−02 43.77 0.173 3.3E−02 28.42 1.04 2.3E−0243.77 NUE545 9484.4 0.163 2.7E−07 79.91 0.194 4.6E−07 43.83 1.30 2.7E−0779.91 Control 0.091 0.135 0.72 NUE549 9341.1 0.118 1.4E−02 30.70 0.1406.0E−01 3.79 0.95 1.4E−02 30.70 Control 0.091 0.135 0.72 NUE563 9454.10.119 9.5E−03 31.19 0.148 1.5E−01 9.88 0.95 9.5E−03 31.19 NUE563 9452.30.104 2.4E−01 15.20 0.140 6.0E−01 4.00 0.84 2.4E−01 15.20 NUE563 9453.40.137 2.1E−04 51.54 0.155 3.6E−02 15.50 1.10 2.1E−04 51.54 Control 0.0910.135 0.72 NUE565 9444.1 0.111 6.2E−02 22.99 0.157 6.4E−02 16.45 0.896.2E−02 22.99 Control 0.091 0.135 0.72 NUE566 9514.3 0.143 3.0E−04 58.150.162 4.2E−02 20.44 1.07 6.9E−04 47.03 NUE566 9513.1 0.118 1.7E−02 30.340.155 4.8E−02 14.92 0.94 1.7E−02 30.34 NUE566 9512.4 0.121 2.4E−02 33.060.156 7.6E−02 15.84 0.96 2.4E−02 33.06 NUE566 9514.1 0.134 1.1E−03 48.170.160 1.1E−02 18.57 1.02 1.0E−02 40.14 Control 0.091 0.135 0.72 NUE5689474.4 0.121 7.0E−03 33.08 0.160 1.2E−02 19.19 0.96 7.0E−03 33.08 NUE5689461.2 0.157 7.6E−06 73.36 0.170 4.1E−03 26.24 1.26 7.6E−06 73.36 NUE5689462.4 0.139 5.3E−04 53.79 0.172 5.8E−03 27.76 1.11 5.3E−04 53.79 NUE5689462.3 0.131 1.4E−03 44.88 0.167 1.3E−03 23.84 1.05 1.4E−03 44.88 NUE5689463.4 0.129 3.7E−03 42.01 0.159 2.5E−02 18.33 1.03 3.7E−03 42.01Control 0.091 0.135 0.72 NUE573 9491.4 0.123 6.0E−03 35.42 0.149 1.7E−0110.44 0.98 6.0E−03 35.42 NUE573 9492.1 0.182 5.7E−09 100.76 0.1901.2E−06 41.04 1.46 5.7E−09 100.76 NUE573 9493.4 0.141 5.6E−05 55.360.166 2.1E−03 23.03 1.06 6.8E−04 45.86 NUE573 9494.3 0.151 5.3E−06 66.870.171 6.6E−04 26.79 1.21 5.3E−06 66.87 NUE573 9491.2 0.139 1.3E−04 53.690.164 1.1E−02 21.53 1.11 1.3E−04 53.69 NUE573 9492.2 0.130 1.5E−03 43.690.165 4.8E−03 22.63 1.04 1.5E−03 43.69 Control 0.091 0.135 0.72 NUE5759501.4 0.172 9.2E−09 89.99 0.198 1.1E−07 47.36 1.38 9.2E−09 89.99 NUE5759504.3 0.095 7.7E−01 4.69 0.135 1.0E+00 −0.04 0.76 7.7E−01 4.69 NUE5759504.1 0.160 4.5E−05 76.22 0.178 3.5E−03 32.48 1.28 4.5E−05 76.22 NUE5759503.1 0.165 4.1E−06 82.22 0.168 1.2E−02 24.58 1.32 4.1E−06 82.22 NUE5759502.1 0.136 9.0E−04 50.03 0.151 1.3E−01 12.57 1.09 9.0E−04 50.03Control 0.091 0.135 0.72 NUE578 9524.3 0.165 6.9E−07 81.99 0.185 2.5E−0437.68 1.32 6.9E−07 81.99 NUE578 9524.1 0.206 3.9E−09 127.63 0.2021.1E−06 49.79 1.56 4.4E−07 115.41 NUE578 9523.3 0.181 4.2E−09 99.400.179 1.9E−05 33.16 1.45 4.2E−09 99.40 NUE578 9522.3 0.141 6.5E−05 55.950.167 1.6E−03 24.01 1.13 6.5E−05 55.95 Control 0.091 0.135 0.72 NUE5809551.3 0.135 3.5E−04 48.50 0.157 2.7E−02 16.48 1.08 3.5E−04 48.50 NUE5809554.2 0.093 8.5E−01 0.130 7.1E−01 0.74 8.5E−01 NUE580 9553.4 0.1371.5E−04 51.17 0.169 8.0E−04 25.59 1.10 1.5E−04 51.17 NUE580 9551.4 0.1652.1E−07 82.42 0.170 1.3E−03 26.33 1.32 2.1E−07 82.42 NUE580 9554.4 0.1381.0E−03 52.73 0.152 9.4E−02 12.99 1.11 1.0E−03 52.73 Control 0.091 0.1350.72 NUE582 9561.1 0.133 2.5E−03 47.08 0.167 6.9E−03 24.32 0.99 7.7E−0336.09 NUE582 9562.1 0.127 3.4E−03 40.44 0.152 7.7E−02 13.15 1.02 3.4E−0340.44 NUE582 9562.4 0.120 2.8E−02 32.82 0.153 1.1E−01 13.90 0.91 1.0E−0126.19 NUE582 9563.3 0.141 2.1E−04 55.33 0.178 4.6E−04 32.62 1.13 2.1E−0455.33 NUE582 9561.2 0.172 9.3E−08 89.88 0.186 1.1E−05 37.84 1.30 4.6E−0679.08 Control 0.091 0.135 0.72

Table 41: Analyses of growth rate (RGR of rosette area and diameter andRGR of plot coverage) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under nitrogen deficientconditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM K₂SO₄, 2 mMCaCl₂ and microelements) as compared to control plants.

The genes presented in Tables 42 and 43, hereinbelow, have improvedplant NUE and produced larger plant biomass when grown under standardnitrogen fertilization conditions, compared to control plants. Inaddition a production of a larger number of leaves as well as a higherplot coverage when grown at low nitrogen conditions indicate a largerphotosynthetic capacity of the plant when grown at high nitrogen growthconditions. Table 42 and 43 depict analyses of rosette area and leafnumber (rosette diameter, rosette area, leaf number, leaf blade area andplot coverage) when grown under standard nitrogen fertilizationconditions (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ andmicroelements) in plants overexpressing the polynucleotides of someembodiments of the invention under the regulation of a constitutivepromoter (35S). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 42 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved rosette growthperformance (rosette diameter and area and plot coverage) under standardnitrogen conditions Gene Rosette Diameter [cm] Rosette Area [cm²] PlotCoverage [%] Name Event # Ave. P-Value % incr. Ave. P-Value % incr. Ave.P-Value % incr. NUE230 9154.2 2.16 1.1E−02 6.87 1.57 4.6E−03 17.45 12.564.6E−03 17.45 Control 2.02 1.34 10.69 NUE234 9163.4 2.40 2.7E−02 18.641.72 1.7E−02 28.84 13.78 1.7E−02 28.84 NUE234 9162.5 2.14 2.1E−02 5.871.52 1.6E−02 13.45 12.13 1.6E−02 13.45 Control 2.02 1.34 10.69 NUE2488983.1 2.21 2.2E−03 9.45 1.58 3.5E−03 18.29 12.65 3.5E−03 18.29 Control2.02 1.34 10.69 NUE249 9122.2 2.27 8.3E−02 12.44 1.62 1.8E−03 20.8312.92 1.8E−03 20.83 Control 2.02 1.34 10.69 NUE268 8996.3 2.17 1.2E−027.34 1.64 2.2E−03 23.05 13.16 2.2E−03 23.05 Control 2.02 1.34 10.69NUE525 9534.1 1.96 1.0E−01 12.33 1.37 4.5E−02 27.78 10.97 4.5E−02 27.78NUE525 9531.2 2.17 8.8E−02 24.47 1.65 7.2E−02 53.32 13.17 7.2E−02 53.32NUE525 9533.1 2.11 3.4E−01 21.09 1.57 3.5E−01 46.56 12.59 3.5E−01 46.56NUE525 9531.3 2.00 2.9E−02 14.68 1.47 1.9E−02 37.33 11.79 1.9E−02 37.33NUE525 9533.4 2.08 3.2E−01 19.24 1.47 3.6E−01 36.69 11.74 3.6E−01 36.69Control 1.75 1.07 8.59 NUE536 9233.3 2.28 2.2E−01 12.83 1.71 1.7E−0127.70 13.66 1.7E−01 27.70 NUE536 9234.1 2.43 6.8E−02 20.35 1.92 2.6E−0243.88 15.39 2.6E−02 43.88 Control 2.02 1.34 10.69 NUE545 9484.2 2.371.2E−03 35.50 1.74 8.5E−03 62.13 13.92 8.5E−03 62.13 NUE545 9482.4 1.936.8E−01 10.29 1.37 5.9E−01 27.95 10.99 5.9E−01 27.95 NUE545 9481.3 2.098.6E−03 19.87 1.51 7.8E−03 40.62 12.08 7.8E−03 40.62 NUE545 9484.4 2.158.4E−02 22.97 1.55 1.7E−01 43.98 12.37 1.7E−01 43.98 Control 1.75 1.078.59 NUE549 9343.6 1.93 4.9E−01 10.73 1.22 6.2E−01 13.86 9.78 6.2E−0113.86 NUE549 9341.1 1.94 5.1E−01 11.16 1.33 4.3E−01 23.54 10.61 4.3E−0123.54 NUE549 9342.3 2.01 2.7E−02 15.03 1.38 4.3E−02 28.53 11.04 4.3E−0228.53 Control 1.75 1.07 8.59 NUE560 9423.4 2.23 4.7E−01 10.17 1.664.2E−01 24.21 13.28 4.2E−01 24.21 Control 2.02 1.34 10.69 NUE568 9461.22.35 2.3E−03 34.72 2.01 1.3E−02 87.26 16.08 1.3E−02 87.26 NUE568 9461.32.05 9.9E−02 17.54 1.48 7.0E−02 38.17 11.87 7.0E−02 38.17 NUE568 9462.41.92 2.1E−01 9.87 1.25 3.4E−01 16.76 9.46 6.7E−01 10.14 NUE568 9463.42.01 7.4E−02 14.90 1.41 7.7E−02 31.41 11.29 7.7E−02 31.41 Control 1.751.07 8.59 NUE573 9491.4 2.10 9.7E−03 20.13 1.48 1.4E−02 37.74 11.831.4E−02 37.74 NUE573 9492.1 2.01 1.0E−01 15.05 1.36 7.2E−02 26.45 8.028.5E−01 −6.67 NUE573 9493.4 1.99 1.3E−01 14.08 1.38 7.7E−02 28.86 10.423.7E−01 21.28 NUE573 9491.2 2.18 1.1E−01 25.09 1.59 1.3E−01 48.09 12.721.3E−01 48.09 NUE573 9494.3 1.98 7.0E−02 13.52 1.45 1.0E−01 34.78 11.571.0E−01 34.78 NUE573 9492.2 1.94 4.4E−01 11.14 1.46 5.2E−01 35.94 9.795.7E−01 14.02 Control 1.75 1.07 8.59 NUE575 9501.4 1.96 2.8E−01 12.361.31 2.7E−01 22.00 10.48 2.7E−01 22.00 NUE575 9504.1 2.13 4.8E−02 21.961.58 7.9E−02 47.31 12.65 7.9E−02 47.31 NUE575 9503.1 1.95 9.7E−02 11.941.24 3.4E−01 15.70 9.94 3.4E−01 15.70 NUE575 9502.1 1.99 4.1E−01 14.241.44 3.8E−01 34.12 10.35 6.9E−01 20.49 Control 1.75 1.07 8.59 NUE5789524.1 2.15 3.4E−03 23.14 1.64 1.9E−03 52.74 13.12 1.9E−03 52.74 NUE5789524.3 2.08 6.0E−01 18.89 1.58 5.2E−01 47.61 12.68 5.2E−01 47.61 NUE5789523.3 2.37 8.6E−02 35.89 1.79 2.9E−02 66.79 13.36 1.4E−03 55.59 NUE5789522.3 2.07 1.3E−01 18.61 1.63 1.2E−01 51.78 13.03 1.2E−01 51.78 Control1.75 1.07 8.59 NUE580 9552.3 1.92 1.1E−01 10.01 1.24 2.4E−01 15.83 9.952.4E−01 15.83 NUE580 9551.3 1.98 4.3E−02 13.26 1.47 1.4E−02 37.05 11.771.4E−02 37.05 NUE580 9554.4 2.03 3.7E−01 16.34 1.52 2.0E−01 41.78 12.182.0E−01 41.78 Control 1.75 1.07 8.59 NUE582 9561.1 2.25 9.2E−02 28.981.73 1.6E−01 61.52 13.87 1.6E−01 61.52 NUE582 9561.2 2.10 7.1E−03 20.111.56 4.6E−03 45.40 12.49 4.6E−03 45.40 Control 1.75 1.07 8.59 NUE5859661.1 2.46 4.9E−02 21.69 2.02 1.2E−05 50.77 16.12 1.2E−05 50.77 Control2.02 1.34 10.69 NUE588 9591.3 2.14 1.3E−01 5.91 1.50 5.3E−02 12.38 12.025.3E−02 12.38 Control 2.02 1.34 10.69

Table 42: Analyses of rosette growth performance (rosette diameter andarea and plot coverage) of transgenic plants overexpressing theexogenous polynucleotides of some embodiments of the invention (usingthe cloned or synthetic genes listed in Table 23 above) under theregulation of a constitutive promoter (35S) when grown under standardnitrogen (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ andmicroelements) as compared to control plants.

TABLE 43 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved rosette growthperformance (leaf number and leaf blade area) under standard nitrogenconditions Leaf Number Leaf Blade Area [cm²] Gene Name Event # Ave.P-Value % incr. Ave. P-Value % incr. NUE230 9154.2 8.75 1.4E−01 4.030.26 7.3E−03 16.16 Control 8.41 0.23 NUE234 9163.4 8.88 4.2E−01 5.520.28 2.9E−02 23.30 NUE234 9162.5 8.06 5.7E−01 −4.14 0.26 9.0E−02 12.41Control 8.41 0.23 NUE248 8983.1 8.75 1.4E−01 4.03 0.25 1.0E−02 11.97Control 8.41 0.23 NUE249 9122.2 9.25 2.2E−02 9.98 0.27 3.5E−03 17.48Control 8.41 0.23 NUE268 8996.3 8.81 1.4E−02 4.78 0.28 6.2E−04 21.22Control 8.41 0.23 NUE525 9534.1 8.88 3.4E−02 9.44 0.23 6.6E−02 20.64NUE525 9531.2 9.06 9.5E−03 11.75 0.26 1.3E−01 39.24 NUE525 9533.1 8.635.6E−01 6.36 0.24 3.4E−01 29.39 NUE525 9531.3 8.69 4.8E−01 7.13 0.232.3E−02 23.65 NUE525 9533.4 8.88 4.8E−01 9.44 0.23 3.5E−01 24.51 Control8.11 0.19 NUE536 9233.3 9.50 1.2E−02 12.95 0.27 2.8E−01 18.94 NUE5369234.1 9.44 6.1E−02 12.21 0.29 7.6E−02 28.74 Control 8.41 0.23 NUE5459484.2 9.31 1.5E−01 14.84 0.28 2.1E−03 47.68 NUE545 9482.4 8.56 6.3E−015.59 0.22 6.6E−01 18.07 NUE545 9481.3 8.06 8.7E−01 −0.58 0.27 1.2E−0341.88 NUE545 9484.4 8.88 1.1E−01 9.44 0.25 8.3E−02 31.54 Control 8.110.19 NUE549 9343.6 8.81 7.7E−02 8.67 0.20 6.6E−01 8.33 NUE549 9341.18.44 6.7E−01 4.05 0.21 6.0E−01 14.37 NUE549 9342.3 9.06 1.1E−01 11.750.21 1.3E−01 13.98 Control 8.11 0.19 NUE560 9423.4 8.75 1.3E−02 4.030.28 4.4E−01 21.95 Control 8.41 0.23 NUE568 9461.2 9.63 1.5E−02 18.690.30 2.1E−02 59.80 NUE568 9461.3 8.94 2.6E−01 10.21 0.23 3.2E−02 25.07NUE568 9462.4 8.48 3.5E−01 4.60 0.21 4.6E−01 13.21 NUE568 9463.4 8.697.2E−02 7.13 0.24 7.0E−02 28.16 Control 8.11 0.19 NUE573 9491.4 8.632.3E−01 6.36 0.23 6.2E−02 22.82 NUE573 9492.1 8.81 7.7E−02 8.67 0.233.9E−02 20.79 NUE573 9493.4 8.86 4.2E−02 9.22 0.22 8.1E−02 17.66 NUE5739491.2 8.63 1.2E−01 6.36 0.25 1.2E−01 33.61 NUE573 9494.3 9.13 1.5E−0112.52 0.23 9.9E−02 20.47 NUE573 9492.2 8.46 7.1E−01 4.30 0.23 5.5E−0125.07 Control 8.11 0.19 NUE575 9501.4 8.38 3.6E−01 3.28 0.21 3.9E−0114.56 NUE575 9504.1 9.06 9.5E−03 11.75 0.25 1.1E−01 33.06 NUE575 9503.18.06 9.2E−01 −0.58 0.22 3.3E−01 16.13 NUE575 9502.1 8.98 3.9E−01 10.730.22 3.0E−01 18.72 Control 8.11 0.19 NUE578 9524.1 8.50 1.9E−01 4.820.26 7.3E−03 40.77 NUE578 9524.3 8.31 8.7E−01 2.50 0.25 5.4E−01 32.70NUE578 9523.3 9.48 1.5E−02 16.93 0.28 4.2E−03 51.09 NUE578 9522.3 9.061.1E−01 11.75 0.24 1.9E−01 27.90 Control 8.11 0.19 NUE580 9552.3 8.561.4E−01 5.59 0.20 4.9E−01 7.20 NUE580 9551.3 8.69 7.2E−02 7.13 0.231.6E−02 25.62 NUE580 9554.4 8.63 4.8E−01 6.36 0.23 3.5E−01 24.90 Control8.11 0.19 NUE582 9561.1 9.44 2.7E−01 16.38 0.27 1.6E−01 43.32 NUE5829561.2 8.94 1.8E−02 10.21 0.25 5.5E−03 33.79 Control 8.11 0.19 NUE5859661.1 8.94 4.0E−03 6.26 0.33 1.8E−02 43.63 Control 8.41 0.23 NUE5889591.3 9.19 5.6E−04 9.24 0.25 2.6E−02 9.62 Control 8.41 0.23

Table 43: Analyses of rosette growth performance (leaf number and leafblade area) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogen (6 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) as comparedto control plants.

The genes presented in Tables 44 and 45, hereinbelow, have improvedplant growth rate when grown at limiting nitrogen fertilization levels.These genes improved the growth rate of the rosette and faster coveredthe soil when grown at standard nitrogen fertilization levels. Thesegenes produced faster growing plants showing a better utilization of thenitrogen present.

Tables 44 and 45 depict analyses of the growth rate of the rosettediameter, rosette area, leaf blade area, leaf number and plot coveragewhen grown under standard nitrogen conditions (6 mM KNO₃, 1 mM KH₂PO₄, 1mM MgSO₄, 2 mM CaCl₂ and microelements) in plants overexpressing thepolynucleotides of some embodiments of the invention under theregulation of a constitutive promoter (35S). Evaluation of each gene wasperformed by testing the performance of several events. Some of thegenes were evaluated in more than one tissue culture assay and theresults obtained were repeated. Event with p-value<0.1 was consideredstatistically significant.

TABLE 44 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved growth rate (RGR ofleaf blade area, leaf number and rosette area) under standard nitrogenconditions RGR Of Leaf Blade Area Gene Event % RGR Of Leaf Number RGR OfRosette Area Name # Ave. P-Value incr. Ave. P-Value % incr. Ave. P-Value% incr. NUE230 9154.2 0.032 2.5E−01 18.08 0.561 0.862 −1.88 0.20 2.8E−0118.63 NUE230 9153.3 0.034 1.5E−01 25.07 0.629 0.404 9.96 0.22 9.2E−0232.65 Control 0.027 0.572 0.16 NUE525 9534.1 0.027 3.4E−01 17.00 0.5770.820 3.62 0.18 2.5E−01 25.20 NUE525 9531.2 0.032 4.9E−02 37.26 0.6190.479 11.17 0.21 2.0E−02 53.67 NUE525 9533.1 0.029 2.1E−01 23.87 0.5700.889 2.25 0.20 6.0E−02 45.89 NUE525 9531.3 0.028 2.3E−01 21.75 0.6180.485 10.96 0.19 9.2E−02 37.52 NUE525 9533.4 0.028 2.4E−01 22.32 0.5810.783 4.35 0.19 1.2E−01 36.12 Control 0.023 0.557 0.14 NUE536 9233.30.032 2.0E−01 21.11 0.696 0.053 21.70 0.21 9.8E−02 30.44 NUE536 9234.10.035 6.2E−02 31.13 0.719 0.025 25.69 0.24 1.6E−02 46.81 Control 0.0270.572 0.16 NUE545 9484.2 0.034 1.4E−02 47.61 0.666 0.224 19.56 0.237.1E−03 63.45 NUE545 9482.4 0.026 5.0E−01 13.65 0.617 0.523 10.85 0.182.8E−01 26.57 NUE545 9481.3 0.032 3.8E−02 39.71 0.478 0.19 7.9E−02 38.94NUE545 9484.4 0.030 1.3E−01 27.62 0.542 0.20 6.8E−02 41.88 Control 0.0230.557 0.14 NUE568 9474.3 0.025 7.0E−01 7.00 0.535 0.15 6.4E−01 10.17NUE568 9471.3 0.024 7.5E−01 5.65 0.627 0.423 12.49 0.17 3.4E−01 20.52NUE568 9461.2 0.037 3.1E−03 58.77 0.665 0.208 19.45 0.26 3.9E−04 88.65NUE568 9474.4 0.026 5.5E−01 10.89 0.526 0.726 −5.61 0.15 6.2E−01 10.72NUE568 9461.3 0.029 1.9E−01 23.81 0.643 0.342 15.47 0.20 8.0E−02 39.53Control 0.023 0.557 0.14 NUE573 9491.4 0.026 4.3E−01 14.05 0.543 0.872−2.57 0.19 1.2E−01 34.11 NUE573 9493.4 0.026 4.9E−01 12.34 0.653 0.27017.18 0.18 2.0E−01 28.04 NUE573 9491.2 0.030 1.0E−01 30.33 0.519 0.657−6.87 0.21 4.2E−02 46.75 NUE573 9492.2 0.029 2.3E−01 24.31 0.531 0.791−4.60 0.19 1.6E−01 35.61 Control 0.023 0.557 0.14 NUE575 9504.1 0.0301.1E−01 30.22 0.624 0.428 12.01 0.21 4.0E−02 47.00 NUE575 9503.1 0.0274.0E−01 15.42 0.516 0.651 −7.29 0.16 4.7E−01 15.58 NUE575 9502.1 0.0264.7E−01 13.38 0.609 0.566 9.32 0.19 1.7E−01 32.29 Control 0.023 0.5570.14 NUE578 9524.1 0.033 3.3E−02 41.00 0.613 0.511 10.12 0.22 1.8E−0254.74 NUE578 9524.3 0.031 1.5E−01 31.93 0.608 0.642 9.18 0.21 7.3E−0249.17 NUE578 9523.3 0.034 1.6E−02 46.45 0.665 0.213 19.44 0.23 5.4E−0365.74 NUE578 9522.3 0.029 2.1E−01 23.38 0.685 0.143 23.02 0.21 2.4E−0252.52 Control 0.023 0.557 0.14 NUE580 9551.3 0.029 1.4E−01 26.91 0.6020.608 8.02 0.19 8.4E−02 38.45 NUE580 9554.4 0.027 3.8E−01 16.77 0.5430.875 −2.57 0.19 9.4E−02 38.64 Control 0.023 0.557 0.14 NUE582 9561.10.032 4.1E−02 39.34 0.666 0.252 19.56 0.22 1.2E−02 60.37 NUE582 9562.10.027 3.5E−01 17.41 0.577 0.833 3.51 0.17 3.0E−01 23.28 NUE582 9562.40.027 3.8E−01 15.92 0.568 0.902 1.94 0.16 4.4E−01 16.65 NUE582 9561.20.032 7.0E−02 37.29 0.665 0.255 19.42 0.22 3.1E−02 54.06 Control 0.0230.557 0.14 NUE585 9661.3 0.030 4.5E−01 11.39 0.684 0.145 19.49 0.185.3E−01 10.62 NUE585 9661.1 0.039 1.2E−02 43.80 0.658 0.178 14.96 0.257.0E−03 52.30 Control 0.027 0.572 0.16 NUE588 9591.3 0.031 2.6E−01 17.230.713 0.052 24.56 0.19 3.2E−01 17.29 Control 0.027 0.572 0.16

Table 44: Analyses of growth rate (RGR of leaf blade area, leaf numberand rosette area) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogen (6 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) as comparedto control plants.

TABLE 45 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved growth rate (RGR ofrosette diameter and plot coverage) under standard nitrogen conditionsRGR Of Rosette Diameter RGR Of Plot Coverage Gene Name Event # Ave.P-Value % incr. Ave. P-Value % incr. NUE230 9154.2 0.20 0.612 5.67 1.560.283 18.63 NUE230 9153.3 0.21 0.382 10.99 1.75 0.092 32.65 Control 0.191.32 NUE525 9534.1 0.18 0.672 6.16 1.40 0.249 25.20 NUE525 9531.2 0.210.188 19.78 1.72 0.020 53.67 NUE525 9533.1 0.21 0.239 18.66 1.63 0.06045.89 NUE525 9531.3 0.20 0.407 12.27 1.54 0.092 37.52 NUE525 9533.4 0.200.308 16.23 1.52 0.125 36.12 Control 0.17 1.12 NUE536 9233.3 0.22 0.25013.45 1.72 0.098 30.44 NUE536 9234.1 0.23 0.068 21.81 1.93 0.016 46.81Control 0.19 1.32 NUE545 9484.2 0.23 0.024 34.81 1.83 0.007 63.45 NUE5459482.4 0.18 0.741 5.45 1.42 0.281 26.57 NUE545 9481.3 0.20 0.304 15.241.55 0.079 38.94 NUE545 9484.4 0.21 0.215 18.52 1.59 0.068 41.88 Control0.17 1.12 NUE568 9474.3 0.18 0.868 2.45 1.23 0.641 10.17 NUE568 9471.30.17 0.853 −2.69 1.35 0.345 20.52 NUE568 9461.2 0.23 0.039 31.20 2.110.000 88.65 NUE568 9474.4 0.18 0.947 0.98 1.24 0.624 10.72 NUE568 9461.30.21 0.218 18.40 1.56 0.080 39.53 Control 0.17 1.12 NUE573 9491.4 0.190.582 8.00 1.50 0.121 34.11 NUE573 9493.4 0.19 0.589 7.89 1.35 0.34920.66 NUE573 9491.2 0.21 0.222 18.90 1.64 0.042 46.75 NUE573 9492.2 0.180.821 3.40 1.27 0.542 13.46 Control 0.17 1.12 NUE575 9504.1 0.21 0.19619.39 1.64 0.040 47.00 NUE575 9503.1 0.19 0.644 6.72 1.29 0.472 15.58NUE575 9502.1 0.19 0.637 7.16 1.33 0.439 19.10 Control 0.17 1.12 NUE5789524.1 0.21 0.154 21.31 1.73 0.018 54.74 NUE578 9524.3 0.21 0.302 19.941.67 0.073 49.17 NUE578 9523.3 0.23 0.036 33.14 1.73 0.017 54.56 NUE5789522.3 0.19 0.472 10.54 1.71 0.024 52.52 Control 0.17 1.12 NUE580 9551.30.20 0.319 14.57 1.55 0.084 38.45 NUE580 9554.4 0.18 0.764 4.75 1.550.094 38.64 Control 0.17 1.12 NUE582 9561.1 0.22 0.112 24.58 1.79 0.01260.37 NUE582 9562.1 0.19 0.548 9.10 1.38 0.300 23.28 NUE582 9562.4 0.190.469 10.79 1.31 0.438 16.65 NUE582 9561.2 0.20 0.325 16.03 1.72 0.03154.06 Control 0.17 1.12 NUE585 9661.3 0.20 0.518 7.29 1.46 0.528 10.62NUE585 9661.1 0.22 0.142 17.93 2.00 0.007 52.30 Control 0.19 1.32 NUE5889591.3 0.21 0.413 9.34 1.54 0.317 17.29 Control 0.19 1.32

Table 45: Analyses of growth rate (RGR of rosette diameter and plotcoverage) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogen (6 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) as comparedto control plants.

Example 7 Assay 3: Nitrogen Use Efficiency Measured Until Bolting Stage:Plant Biomass and Plant Growth Rate at Limited and Standard NitrogenConcentration Under Greenhouse Conditions

This assay follows seed yield production, the biomass formation and therosette area growth of plants grown in the greenhouse at limiting andnon-limiting nitrogen growth conditions. Transgenic Arabidopsis seedswere sown in agar media supplemented with ½ MS medium and a selectionagent (Kanamycin). The T₂ transgenic seedlings were then transplanted to1.7 trays filled with peat and perlite in a 1:1 ratio. The trays wereirrigated with a solution containing nitrogen limiting conditions, whichwere achieved by irrigating the plants with a solution containing 1.5 mMinorganic nitrogen in the form of KNO₃, supplemented with 1 mM KH₂PO₄, 1mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ and microelements, while normalnitrogen levels were achieved by applying a solution of 6 mM inorganicnitrogen also in the form of KNO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mMCaCl₂ and microelements. All plants were grown in the greenhouse untilmature seeds. Plant biomass (the above ground tissue) was weightimmediately after harvesting the rosette (plant fresh weight [FW]).Following, plants were dried in an oven at 50° C. for 48 hours andweighted (plant dry weight [DW]).

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying the35S promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, freshweight and dry matter. Transgenic plants performance was compared tocontrol plants grown in parallel under the same conditions.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) was used for capturingimages of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. During the captureprocess, the tubes were placed beneath the iron mount, while avoidingdirect sun light and casting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, rosette area, rosette diameter, leaf blade area, plot coverageand leaf petiole area.

Vegetative growth rate: is the rate of growth of the plant as defined byformula VIII, IX, X and XI as described above:Relative growth rate of leaf blade area=Regression coefficient of leafarea along time course.  Formula VIII:Relative growth rate of rosette area=Regression coefficient of rosettearea along time course.  Formula IX:Relative growth rate of rosette diameter=Regression coefficient ofrosette diameter along time course.  Formula XRelative growth rate of plot coverage=Regression coefficient of plotcoverage along time course.  Formula XI

Plant Fresh and Dry Weight—

On about day 40 from sowing, the plants were harvested and directlyweight for the determination of the plant fresh weight (FW) and left todry at 50° C. in a drying chamber for about 48 hours before weighting todetermine plant dry weight (DW).

Statistical Analyses—

To identify genes conferring significantly improved NUE, the resultsobtained from the transgenic plants were compared to those obtained fromcontrol plants. To identify outperforming genes and constructs, resultsfrom the independent transformation events tested are analyzedseparately. Data was analyzed using Student's t-test and results wereconsidered significant if the p value was less than 0.1. The JMPstatistics software package was used (Version 5.2.1, SAS Institute Inc.,Cary, N.C., USA).

Experimental Results:

The genes presented in Tables 46 and 47, hereinbelow, have improvedplant NUE when grown under limiting nitrogen growth conditions, comparedto control plants. These genes produced larger plants with a largerphotosynthetic capacity when grown under limiting nitrogen conditions.

Tables 46 and 47 depict analyses of plant biomass and photosyntheticarea (fresh weight, dry weight, rosette diameter, rosette area and plotcoverage) when grown under limiting nitrogen conditions (1.5 mM KNO₃, 1mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ and microelements) inplants overexpressing the polynucleotides of some embodiments of theinvention under the regulation of a constitutive promoter (35S).Evaluation of each gene was performed by testing the performance ofseveral events. Some of the genes were evaluated in more than one tissueculture assay and the results obtained were repeated. Event withp-value<0.1 was considered statistically significant.

TABLE 46 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (dryweight and fresh weight) under limiting nitrogen conditions Dry Weight[g] Fresh Weight [g] Gene % % Name Event # Average P-Value incrementAverage P-Value increment NUE227 9851.4 0.076 0.014 44.26 0.725 0.00279.65 NUE227 9854.2 0.059 0.202 12.33 0.569 0.000 40.93 NUE227 9853.10.069 0.025 31.25 0.581 0.092 44.03 NUE227 9851.1 0.063 0.052 19.430.581 0.205 44.03 NUE227 9852.3 0.063 0.063 18.24 0.519 0.469 28.54Control 0.053 0.404 NUE233 10173.5 0.035 0.055 34.48 0.243 0.838 −1.89Control 0.026 0.248 NUE256 10061.4 0.034 0.031 32.08 NUE256 10061.10.044 0.126 70.50 0.325 0.250 31.29 Control 0.026 0.248 NUE512 9284.20.044 0.417 −17.23 0.419 0.794 3.76 NUE512 9283.1 0.066 0.033 25.340.488 0.088 20.80 NUE512 9284.3 0.052 0.900 −1.86 0.469 0.566 16.15NUE512 9282.3 0.064 0.066 20.61 0.506 0.005 25.44 NUE512 9283.3 0.0630.247 18.24 0.538 0.002 33.19 NUE512 9281.3 0.079 0.066 49.32 0.5380.023 33.41 Control 0.053 0.404 NUE514 9403.5 0.050 0.581 −5.41 0.3880.807 −3.98 NUE514 9404.4 0.054 0.964 2.20 0.439 0.759 8.85 NUE5149402.2 0.056 0.468 6.42 0.594 0.000 47.12 NUE514 9402.5 0.057 0.553 7.600.488 0.088 20.80 Control 0.053 0.404 NUE531 10082.2 0.042 0.001 62.840.235 0.976 −5.26 NUE531 10081.5 0.029 0.810 10.46 0.219 0.334 −11.63Control 0.026 0.248 NUE532 9222.4 0.069 0.066 31.25 0.606 0.296 50.22NUE532 9222.3 0.061 0.773 15.88 0.444 0.148 9.96 NUE532 9222.1 0.0640.570 20.61 0.525 0.117 30.09 NUE532 9223.3 0.062 0.093 17.06 0.4750.603 17.70 NUE532 9224.4 0.035 0.007 −32.94 0.467 0.037 15.71 NUE5329223.5 0.057 0.746 7.60 0.556 0.242 37.83 Control 0.053 0.404 NUE5359086.2 0.056 0.915 5.24 0.550 0.477 36.28 Control 0.053 0.404 NUE5379392.2 0.053 0.988 0.51 0.550 0.021 36.28 NUE537 9393.2 0.055 0.847 3.720.589 0.065 46.02 NUE537 9393.1 0.060 0.274 13.51 0.444 0.148 9.96NUE537 9393.3 0.068 0.033 28.89 0.575 0.014 42.48 Control 0.053 0.404NUE576 9794.1 0.039 0.090 48.89 0.294 0.028 18.67 Control 0.026 0.248NUE576 9791.3 0.431 0.838 6.86 NUE576 9792.4 0.500 0.007 23.89 NUE5769792.3 0.550 0.159 36.28 Control 0.404

Table 46: Analyses of plant biomass (dry and fresh weight) of transgenicplants overexpressing the exogenous polynucleotides of some embodimentsof the invention (using the cloned or synthetic genes listed in Table 23above) under the regulation of a constitutive promoter (35S) when grownunder limiting nitrogen (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mMKCl, 2 mM CaCl₂ and microelements) as compared to control plants.

TABLE 47 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass(rosette diameter and area and plot coverage) under limiting nitrogenconditions Rosette Diameter [cm] Rosette Area [cm²] Plot Coverage [%]Gene P- % % P- % Name Event # Ave. Value incr. Ave. P-Value incr. Ave.Value incr. NUE227 9851.4 2.040 2.4E−02 48.28 1.477 7.6E−02 115.9011.819 7.6E−02 115.90 NUE227 9854.2 1.778 5.9E−02 29.24 1.070 7.0E−0356.44 8.564 7.0E−03 56.44 NUE227 9853.1 1.679 9.5E−02 22.03 0.9791.1E−01 43.09 7.833 1.1E−01 43.09 NUE227 9851.1 1.555 2.8E−01 13.030.915 2.8E−01 33.71 7.320 2.8E−01 33.71 NUE227 9852.3 1.889 1.7E−0137.33 1.298 1.8E−01 89.71 10.386 1.8E−01 89.71 Control 1.376 0.684 5.474NUE233 10173.5 1.778 1.9E−01 7.66 1.080 3.2E−01 16.76 7.559 7.1E−01 5.15Control 1.651 0.925 7.189 NUE256 10061.1 1.991 2.6E−03 20.56 1.3332.7E−02 44.13 10.664 2.0E−02 48.34 Control 1.651 0.925 7.189 NUE5129284.2 1.475 7.1E−01 7.21 0.794 7.0E−01 15.97 6.349 7.0E−01 15.97 NUE5129283.1 1.581 1.8E−01 14.91 0.916 2.6E−02 33.86 7.328 2.6E−02 33.86NUE512 9284.3 1.395 9.0E−01 1.41 0.719 8.2E−01 5.14 5.755 8.2E−01 5.14NUE512 9282.3 1.588 2.6E−01 15.43 0.928 6.3E−02 35.67 7.427 6.3E−0235.67 NUE512 9283.3 1.410 8.2E−01 2.50 0.789 3.9E−01 15.37 6.315 3.9E−0115.37 NUE512 9281.3 1.526 3.8E−01 10.94 0.824 4.1E−01 20.42 6.2366.5E−01 13.91 Control 1.376 0.684 5.474 NUE514 9403.5 1.581 5.6E−0114.93 0.901 5.4E−01 31.69 7.209 5.4E−01 31.69 NUE514 9404.4 1.6082.3E−01 16.92 0.930 2.0E−01 35.86 6.923 2.2E−02 26.47 NUE514 9402.21.996 1.8E−01 45.07 1.395 1.5E−01 103.85 11.159 1.5E−01 103.85 NUE5149402.5 1.860 3.2E−01 35.21 1.160 3.2E−01 69.52 9.280 3.2E−01 69.52NUE514 9404.5 1.689 2.2E−01 22.77 1.006 4.7E−02 47.02 8.048 4.7E−0247.02 Control 1.376 0.684 5.474 NUE527 9201.1 1.773 1.4E−02 28.89 1.1099.3E−02 62.12 8.875 9.3E−02 62.12 Control 1.376 0.684 5.474 NUE53110081.5 1.758 4.5E−01 6.49 1.043 5.1E−01 12.74 8.342 4.3E−01 16.04Control 1.651 0.925 7.189 NUE532 9222.4 1.752 3.9E−01 27.34 1.0564.4E−01 54.32 8.448 4.4E−01 54.32 NUE532 9222.3 1.668 4.6E−04 21.241.034 8.3E−05 51.08 8.270 8.3E−05 51.08 NUE532 9222.1 1.623 3.8E−0117.95 1.016 2.8E−01 48.52 8.130 2.8E−01 48.52 NUE532 9223.3 1.5855.5E−03 15.23 0.902 6.2E−03 31.88 7.219 6.2E−03 31.88 NUE532 9224.41.732 1.4E−04 25.88 1.060 6.5E−05 54.91 7.941 2.3E−02 45.06 NUE5329223.5 1.899 7.1E−02 38.02 1.294 3.2E−02 89.11 10.353 3.2E−02 89.11Control 1.376 0.684 5.474 NUE535 9086.2 1.696 2.2E−01 23.27 0.9382.2E−01 37.03 7.502 2.2E−01 37.03 NUE535 9084.2 1.463 6.4E−01 6.35 0.8274.3E−01 20.92 6.620 4.3E−01 20.92 NUE535 9081.1 1.521 3.3E−01 10.570.823 3.0E−01 20.21 6.581 3.0E−01 20.21 NUE535 9082.1 1.432 5.6E−01 4.100.742 3.8E−01 8.46 5.938 3.8E−01 8.46 Control 1.376 0.684 5.474 NUE5379391.1 1.503 5.2E−01 9.24 0.807 3.8E−01 17.86 6.452 3.8E−01 17.86 NUE5379392.2 1.475 4.9E−01 7.21 0.851 3.4E−01 24.32 6.806 3.4E−01 24.32 NUE5379393.2 1.532 8.5E−03 11.34 0.955 4.1E−04 39.59 7.157 4.5E−02 30.73NUE537 9393.1 1.856 1.7E−03 34.95 1.225 7.0E−05 78.96 9.797 7.0E−0578.96 NUE537 9392.3 1.429 8.3E−01 3.89 0.784 7.3E−01 14.54 6.270 7.3E−0114.54 NUE537 9393.3 1.739 5.3E−02 26.42 1.092 1.5E−02 59.52 8.7331.5E−02 59.52 Control 1.376 0.684 5.474 NUE576 9794.1 1.963 1.5E−0118.86 1.350 2.3E−01 45.96 10.800 2.1E−01 50.23 Control 1.651 0.925 7.189NUE576 9791.3 1.416 8.6E−01 2.94 0.753 7.4E−01 10.01 6.022 7.4E−01 10.01NUE576 9792.4 1.826 2.1E−05 32.75 1.204 2.4E−04 75.90 9.629 2.4E−0475.90 NUE576 9792.3 1.912 5.4E−06 39.03 1.208 1.1E−05 76.46 9.6601.1E−05 76.46 Control 1.376 0.684 5.474

Table 47: Analyses of plant biomass (rosette diameter and area and plotcoverage) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under limiting nitrogen (1.5 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ and microelements)as compared to control plants.

The genes presented in Table 48, hereinbelow, have improved plant NUEwhen grown under limiting nitrogen growth conditions, compared tocontrol plants. These genes produced larger photosynthetic areas as itcan be observed by their larger leaf number, leaf blade area and petiolearea.

Table 48 depicts analyses of plant photosynthetic area (leaf number,leaf blade area and petiole area) when grown under limiting nitrogenconditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂and microelements) in plants overexpressing the polynucleotides of someembodiments of the invention under the regulation of a constitutivepromoter (35S)). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 48 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved photosynthetic area(leaf number, leaf blade area and petiole area) under limiting nitrogenconditions Leaf Petiole Length Leaf Number Leaf Blade Area [cm²] [cm]Gene P- % P- % P- % Name Event # Ave. Value incr. Ave. Value incr. Ave.Value incr. NUE227 9851.4 8.56 1.9E−02 16.38 0.24 4.6E−02 108.44 0.386.5E−02 69.59 NUE227 9854.2 7.63 1.5E−01 3.64 0.19 5.8E−03 67.63 0.321.2E−04 41.44 NUE227 9853.1 7.81 3.5E−02 6.19 0.17 2.2E−01 49.04 0.263.8E−02 14.04 NUE227 9851.1 7.69 2.8E−01 4.49 0.16 2.9E−01 35.75 0.244.7E−01 5.61 NUE227 9852.3 8.50 2.4E−01 15.53 0.21 1.1E−01 82.96 0.324.4E−01 41.85 Control 7.36 0.11 0.22 NUE233 10173.5 7.79 6.0E−01 2.050.19 4.0E−01 15.50 0.26 5.8E−01 8.27 Control 7.63 0.17 0.24 NUE25610061.1 8.13 2.5E−01 6.50 0.23 8.4E−02 39.00 0.31 2.0E−01 30.30 Control7.63 0.17 0.24 NUE512 9283.1 7.63 2.5E−01 3.64 0.16 7.0E−02 42.74 0.241.2E−01 8.56 NUE512 9284.3 6.88 5.0E−01 −6.55 0.13 4.9E−01 15.53 0.218.3E−01 −5.04 NUE512 9282.3 7.38 9.8E−01 0.24 0.16 3.9E−02 41.04 0.265.5E−01 14.40 NUE512 9283.3 7.56 6.3E−01 2.79 0.13 6.1E−01 12.00 0.256.2E−02 11.05 NUE512 9281.3 7.08 7.9E−01 −3.76 0.15 3.8E−02 31.49 0.239.9E−01 0.30 Control 7.36 0.11 0.22 NUE514 9403.5 7.31 8.7E−01 −0.610.16 5.0E−01 35.23 0.28 4.7E−01 25.62 NUE514 9404.4 8.04 4.1E−01 9.220.14 1.2E−01 25.05 0.26 1.1E−02 17.01 NUE514 9402.2 8.75 2.5E−01 18.930.22 1.4E−01 89.22 0.35 3.1E−01 55.03 NUE514 9402.5 8.38 2.7E−01 13.830.19 3.3E−01 63.94 0.32 3.1E−01 42.26 NUE514 9404.5 8.56 1.0E−01 16.380.16 3.9E−02 37.00 0.30 3.7E−01 31.67 Control 7.36 0.11 0.22 NUE5279201.1 8.19 2.0E−03 11.29 0.17 1.3E−01 49.68 0.33 1.7E−03 45.43 NUE5279201.2 6.94 6.5E−01 −5.70 0.12 5.8E−01 7.58 0.22 9.3E−01 −1.31 Control7.36 0.11 0.22 NUE531 10082.2 8.24 7.1E−02 7.98 0.15 2.3E−01 −12.84 0.273.9E−01 13.71 NUE531 10081.5 8.31 4.4E−02 8.95 0.17 9.4E−01 1.15 0.273.2E−02 15.99 Control 7.63 0.17 0.24 NUE532 9222.4 7.56 8.4E−01 2.790.17 4.2E−01 51.79 0.30 4.1E−01 32.08 NUE532 9222.3 8.31 3.5E−02 12.990.16 2.5E−05 41.75 0.29 1.5E−03 30.67 NUE532 9222.1 7.94 1.1E−01 7.890.16 2.6E−01 40.64 0.27 5.0E−01 21.12 NUE532 9223.3 7.31 9.4E−01 −0.610.15 2.6E−01 32.70 0.28 1.5E−03 26.20 NUE532 9224.4 8.27 1.3E−03 12.380.16 3.4E−02 41.63 0.32 6.8E−02 43.77 NUE532 9223.5 8.25 9.8E−02 12.140.20 1.5E−02 72.88 0.35 1.5E−02 58.06 Control 7.36 0.11 0.22 NUE5359086.2 7.75 5.7E−01 5.34 0.16 2.0E−01 37.02 0.34 2.1E−01 52.44 NUE5359084.2 6.88 4.0E−01 −6.55 0.15 2.4E−01 28.20 0.23 8.7E−01 2.43 NUE5359081.1 8.31 1.4E−01 12.99 0.13 4.7E−01 12.91 0.28 2.6E−03 25.65 NUE5359082.1 7.25 6.2E−01 −1.46 0.13 2.5E−02 12.04 0.26 4.5E−01 14.26 Control7.36 0.11 0.22 NUE537 9391.1 7.81 4.8E−01 6.19 0.14 2.6E−01 23.28 0.255.7E−01 11.88 NUE537 9392.2 7.31 9.4E−01 −0.61 0.14 2.9E−01 25.68 0.264.1E−01 14.53 NUE537 9393.2 7.56 7.2E−01 2.79 0.15 2.2E−02 33.42 0.245.1E−01 4.72 NUE537 9393.1 8.63 1.7E−03 17.23 0.19 4.6E−02 69.46 0.344.7E−04 52.73 NEU537 9392.3 7.19 8.5E−01 −2.31 0.13 7.3E−01 10.02 0.257.4E−01 11.68 NUE537 9393.3 8.00 4.8E−01 8.74 0.18 2.6E−02 56.70 0.303.1E−04 33.39 Control 7.36 0.11 0.22 NUE576 9794.1 8.13 5.7E−01 6.500.24 1.4E−01 42.20 0.30 5.0E−01 28.80 Control 7.63 0.17 0.24 NUE5769791.3 7.00 6.7E−01 −4.85 0.13 6.3E−01 15.21 0.24 8.0E−01 7.24 NUE5769792.4 8.75 1.1E−03 18.93 0.18 1.4E−04 55.06 0.35 2.8E−02 56.82 NUE5769792.3 8.06 2.1E−01 9.59 0.20 2.9E−05 71.82 0.34 9.6E−04 52.50 Control7.36 0.11 0.22

Table 48: Analyses of photosynthetic area (leaf number, leaf blade areaand petiole area) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under limiting nitrogen (1.5 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ and microelements)as compared to control plants.

The genes presented in Table 49, hereinbelow, have improved plant growthrate when grown at limiting nitrogen fertilization levels. These genesimproved the growth rate of the rosette and faster covered the soil whengrown under limiting nitrogen growth conditions, compared to controlplants. These genes produced faster growing plants showing a betterutilization of the nitrogen present.

Table 49 depicts analyses of the growth rate of the rosette diameter,rosette area, leaf blade area, leaf number and plot coverage when grownunder standard nitrogen conditions when grown under limiting nitrogenconditions (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂and microelements) in plants overexpressing the polynucleotides of someembodiments of the invention under the regulation of a constitutivepromoter (35S). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 49 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved rosette growthperformance (RGR of rosette area and diameter and plot coverage) underlimiting nitrogen conditions RGR Of Rosette RGR Of Rosette Area DiameterRGR Of Plot Coverage Gene P- % P- % P- % Name Event # Ave. Value incr.Ave. Value incr. Ave. Value incr. NUE227 9851.4 0.183 2.6E−05 118.890.189 2.0E−03 44.11 1.462 2.6E−05 118.89 NUE227 9854.2 0.133 7.4E−0359.50 0.172 2.0E−02 31.13 1.066 7.4E−03 59.50 NUE227 9853.1 0.1204.6E−02 43.12 0.157 1.2E−01 19.92 0.956 4.6E−02 43.12 NUE227 9851.10.112 1.2E−01 33.76 0.152 2.5E−01 15.45 0.894 1.2E−01 33.76 NUE2279852.3 0.161 5.4E−04 93.37 0.174 2.5E−02 32.77 1.292 5.4E−04 93.37Control 0.084 0.131 0.668 NUE256 10063.4 0.132 5.2E−01 11.42 0.1327.1E−01 −6.29 0.923 9.8E−01 0.41 NUE256 10061.1 0.167 1.2E−02 40.750.160 4.0E−01 13.13 1.332 1.2E−02 44.97 Control 0.118 0.141 0.919 NUE5129284.2 0.100 3.9E−01 19.99 0.142 5.7E−01 8.06 0.802 3.9E−01 19.99 NUE5129283.1 0.115 7.3E−02 37.78 0.150 2.7E−01 14.31 0.920 7.3E−02 37.78NUE512 9284.3 0.091 6.8E−01 8.61 0.136 7.8E−01 3.59 0.726 6.8E−01 8.61NUE512 9282.3 0.116 6.6E−02 38.73 0.151 2.8E−01 14.64 0.927 6.6E−0238.73 NUE512 9283.3 0.098 4.0E−01 17.73 0.125 6.8E−01 −5.14 0.7874.0E−01 17.73 NUE512 9281.3 0.103 2.5E−01 23.92 0.140 6.0E−01 6.87 0.7834.2E−01 17.24 Control 0.084 0.131 0.668 NUE514 9403.5 0.108 2.4E−0128.84 0.137 7.8E−01 4.40 0.861 2.4E−01 28.84 NUE514 9404.4 0.117 6.4E−0239.59 0.155 1.6E−01 17.67 0.869 1.4E−01 30.04 NUE514 9402.2 0.1778.9E−05 111.43 0.195 1.5E−03 48.26 1.412 8.9E−05 111.43 NUE514 9403.20.105 3.4E−01 26.02 0.140 7.1E−01 6.57 0.842 3.4E−01 26.02 NUE514 9402.50.144 8.3E−03 72.44 0.180 2.7E−02 37.10 1.152 8.3E−03 72.44 NUE5149404.5 0.126 1.8E−02 50.85 0.164 6.6E−02 24.82 1.008 1.8E−02 50.85Control 0.084 0.131 0.668 NUE527 9201.1 0.131 1.5E−02 56.83 0.1463.8E−01 11.45 1.048 1.5E−02 56.83 Control 0.084 0.131 0.668 NUE5329222.4 0.132 3.4E−02 58.62 0.177 4.2E−02 34.79 1.060 3.4E−02 58.62NUE532 9222.3 0.124 2.7E−02 48.99 0.148 3.2E−01 12.66 0.995 2.7E−0248.99 NUE532 9222.1 0.124 3.9E−02 48.48 0.153 2.5E−01 16.37 0.9923.9E−02 48.48 NUE532 9223.3 0.106 2.0E−01 26.75 0.135 8.3E−01 2.62 0.8472.0E−01 26.75 NUE532 9224.4 0.132 9.5E−03 58.37 0.159 1.0E−01 21.080.991 2.7E−02 48.37 NUE532 9223.5 0.162 1.4E−04 94.31 0.169 3.4E−0228.67 1.298 1.4E−04 94.31 Control 0.084 0.131 0.668 NUE535 9086.2 0.1186.7E−02 40.98 0.169 3.7E−02 28.72 0.942 6.7E−02 40.98 NUE535 9084.20.103 2.6E−01 23.65 0.138 7.0E−01 4.93 0.826 2.6E−01 23.65 NUE535 9081.10.101 3.1E−01 20.62 0.137 7.2E−01 4.66 0.806 3.1E−01 20.62 Control 0.0840.131 0.668 NUE537 9391.1 0.101 3.0E−01 20.89 0.143 5.1E−01 8.62 0.8083.0E−01 20.89 NUE537 9392.2 0.107 1.9E−01 27.77 0.142 5.0E−01 8.29 0.8541.9E−01 27.77 NUE537 9393.2 0.120 4.4E−02 43.18 0.135 8.3E−01 2.61 0.8941.1E−01 33.89 NUE537 9393.1 0.156 5.1E−04 86.73 0.198 2.4E−04 50.581.247 5.1E−04 86.73 NUE537 9392.3 0.101 3.8E−01 20.36 0.140 6.6E−01 6.280.804 3.8E−01 20.36 NUE537 9393.3 0.133 1.0E−02 59.56 0.162 7.9E−0223.54 1.066 1.0E−02 59.56 Control 0.084 0.131 0.668 NUE576 9793.4 0.1631.1E−01 37.49 0.157 5.5E−01 11.44 1.139 3.0E−01 23.91 NUE576 9792.40.139 2.6E−01 17.71 0.167 2.7E−01 18.29 1.047 4.2E−01 13.98 NUE5769794.1 0.168 1.7E−02 41.95 0.161 4.0E−01 14.09 1.343 1.6E−02 46.20Control 0.118 0.141 0.919 NUE576 9792.4 0.150 8.5E−04 79.96 0.1626.5E−02 23.36 1.202 8.5E−04 79.96 NUE576 9792.3 0.149 1.2E−03 78.960.177 1.3E−02 34.59 1.196 1.2E−03 78.96 NUE576 9794.1 0.095 5.1E−0114.23 0.145 4.7E−01 10.57 0.763 5.1E−01 14.23 NUE576 9793.3 0.1042.4E−01 24.59 0.140 6.4E−01 6.33 0.771 4.4E−01 15.45 Control 0.084 0.1310.668

Table 49: Analyses of rosette growth performance (RUR of rosette areaand diameter and plot coverage) of transgenic plants overexpressing theexogenous polynucleotides of some embodiments of the invention (usingthe cloned or synthetic genes listed in Table 23 above) under theregulation of a constitutive promoter (35S) when grown under limitingnitrogen (1.5 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂and microelements) as compared to control plants.

The genes presented in Tables 50 and 51, hereinbelow, have improvedplant NUE when grown under standard nitrogen growth conditions, comparedto control plants. These genes produced larger plants with a largerphotosynthetic area when grown under standard nitrogen growthconditions, compared to control plants.

Tables 50 and 51 depicts analyses of plant biomass (fresh weight, dryweight, rosette diameter, rosette area and plot coverage) when grownunder standard nitrogen conditions (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄,2 mM CaCl₂ and microelements) in plants overexpressing thepolynucleotides of some embodiments of the invention under theregulation of a constitutive promoter (35S)). Evaluation of each genewas performed by testing the performance of several events. Some of thegenes were evaluated in more than one tissue culture assay and theresults obtained were repeated. Event with p-value<0.1 was consideredstatistically significant.

TABLE 50 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass (dryweight and fresh weight) under standard nitrogen conditions Dry WeightFresh Weight Gene Name Event # Ave. P-Value % increment Ave. P-Value %increment NUE227 9851.4 0.170 1.8E−01 18.41 1.631 2.1E−01 12.64 NUE2279854.2 0.163 1.7E−01 13.18 1.744 2.1E−02 20.41 NUE227 9853.1 0.2022.1E−01 40.61 2.019 1.9E−02 39.40 NUE227 9852.3 0.199 2.6E−01 38.871.794 3.7E−01 23.86 Control 0.144 1.448 NUE233 10174.3 0.128 1.3E−0121.58 1.206 1.0E−01 19.80 NUE233 10173.7 0.143 1.4E−02 36.31 1.2103.8E−01 20.15 Control 0.105 1.007 NUE256 10063.4 0.139 4.0E−01 32.651.363 1.6E−01 35.31 NUE256 10061.3 0.118 5.8E−01 12.64 1.025 9.2E−011.80 Control 0.105 1.007 NUE512 9282.3 0.177 2.0E−01 23.20 1.881 2.8E−0329.90 Control 0.144 1.448 NUE514 9403.5 0.168 2.3E−02 17.10 1.5565.9E−01 7.46 NUE514 9402.2 0.161 2.2E−01 11.88 1.769 3.7E−02 22.13NUE514 9404.5 0.153 5.0E−01 6.65 1.531 3.9E−01 5.73 NUE514 9402.5 0.1711.7E−01 19.28 1.488 7.3E−01 2.71 Control 0.144 1.448 NUE531 10081.50.115 5.2E−01 10.00 1.086 5.9E−01 7.83 Control 0.105 1.007 NUE532 9222.40.175 4.9E−01 21.89 1.750 3.4E−01 20.84 NUE532 9223.3 0.156 2.3E−01 8.831.556 2.8E−01 7.46 NUE532 9223.5 0.164 4.4E−01 14.05 1.669 5.3E−02 15.23Control 0.144 1.448 NUE537 9391.1 0.178 1.3E−01 24.07 1.669 4.8E−0215.23 NUE537 9393.1 0.168 2.4E−01 16.92 1.743 3.2E−02 20.35 Control0.144 1.448

Table 50: Analyses of plant biomass (dry weight and fresh weight) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under standard nitrogen (6 mM KNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄,2 mM CaCl₂ and microelements) as compared to control plants.“Ave.”=average.

TABLE 51 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant biomass(rosette diameter and area and plot coverage) under standard nitrogenconditions Gene Rosette Diameter Rosette Area Plot Coverage Name Event #Ave. P-Value % incr. Ave P-Value % incr. Ave. P-Value % incr. NUE2279851.4 1.82 8.6E−01 −1.70 1.29 3.5E−01 9.98 10.30 3.5E−01 9.98 NUE2279854.2 2.13 7.6E−02 14.82 1.40 2.1E−01 19.82 11.22 2.1E−01 19.82 NUE2279853.1 1.96 6.1E−01 5.70 1.45 1.8E−01 23.64 11.58 1.8E−01 23.64 NUE2279852.3 1.94 5.9E−01 4.72 1.40 5.8E−01 19.24 11.17 5.8E−01 19.24 Control1.85 1.17 9.37 NUE233 10174.3 2.69 2.8E−01 12.27 2.54 3.0E−01 24.7320.36 2.7E−01 27.64 NUE233 10173.7 2.61 4.7E−01 9.02 2.49 4.0E−01 22.0018.84 5.9E−01 18.14 Control 2.40 2.04 15.95 NUE256 10063.4 3.37 1.5E−0340.59 3.25 1.4E−01 59.40 24.17 2.9E−02 51.58 NUE256 10061.3 2.89 3.3E−0120.50 2.38 5.8E−01 16.48 19.01 5.3E−01 19.20 Control 2.40 2.04 15.95NUE512 9282.3 2.08 3.8E−02 12.43 1.38 1.3E−01 18.07 11.06 1.3E−01 18.07Control 1.85 1.17 9.37 NUE514 9403.5 2.14 1.9E−02 15.56 1.44 6.0E−0223.37 11.56 6.0E−02 23.37 NUE514 9402.2 2.03 9.8E−02 9.60 1.46 5.4E−0224.90 11.70 5.4E−02 24.90 NUE514 9404.5 2.13 2.1E−02 14.76 1.56 1.6E−0232.88 12.45 1.6E−02 32.88 NUE514 9402.5 2.15 3.9E−02 16.09 1.45 5.6E−0223.83 11.60 5.6E−02 23.83 Control 1.85 1.17 9.37 NUE531 10081.5 2.781.5E−01 16.08 2.50 2.5E−01 22.65 18.66 3.0E−01 17.01 NUE531 10083.2 2.996.6E−02 24.72 3.18 2.0E−01 56.02 24.12 3.4E−01 51.24 Control 2.40 2.0415.95 NUE532 9222.4 2.04 2.8E−01 10.29 1.43 2.2E−01 22.08 11.44 2.2E−0122.08 NUE532 9223.3 2.04 9.0E−02 10.28 1.38 1.3E−01 18.26 11.08 1.3E−0118.26 NUE532 9223.5 2.23 5.3E−03 20.20 1.64 6.4E−03 40.20 13.13 6.4E−0340.20 Control 1.85 1.17 9.37 NUE537 9391.1 1.87 8.6E−01 0.96 1.226.7E−01 4.43 9.78 6.7E−01 4.43 NUE537 9393.1 1.90 7.6E−01 2.36 1.344.7E−01 14.85 10.17 7.5E−01 8.55 Control 1.85 1.17 9.37

Table 51: Analyses of plant biomass (rosette diameter and area and plotcoverage) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogen (6 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) as comparedto control plants. “Incr.”=increment; “Ave.”=average.

The genes presented in Table 52, hereinbelow, have improved plant NUEwhen grown under standard nitrogen growth conditions, compared tocontrol plants. These genes produced larger photosynthetic areas as itcan be observed by their larger leaf number, leaf blade area and petiolearea as compared to control plants.

Table 52 depicts analyses of plant photosynthetic area (leaf number andpetiole area) when grown under standard nitrogen conditions (6 mM KNO₃,1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) in plantsoverexpressing the polynucleotides of some embodiments of the inventionunder the regulation of a constitutive promoter (35S)). Evaluation ofeach gene was performed by testing the performance of several events.Some of the genes were evaluated in more than one tissue culture assayand the results obtained were repeated. Event with p-value<0.1 wasconsidered statistically significant.

TABLE 52 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved photosynthetic areas(leaf blade area and leaf petiole length) under standard nitrogen growthconditions Leaf Blade Area [cm²] Leaf Petiole Length Gene Name Event #Ave. P-Value % increment Ave. P-Value % increment NUE227 9851.4 0.2191.6E−01 15.14 0.305 7.2E−02 −14.52 NUE227 9854.2 0.249 2.6E−02 30.940.373 4.6E−01 4.52 NUE227 9853.1 0.248 6.6E−02 30.68 0.364 7.9E−01 2.16NUE227 9852.3 0.232 4.4E−01 22.31 0.331 4.7E−01 −7.20 Control 0.1900.357 NUE233 10174 0.437 1.8E−01 28.66 0.424 3.3E−01 15.67 NUE233 101740.440 1.8E−01 29.28 0.440 2.3E−01 20.06 Control 0.340 0.366 NUE256 100630.518 2.3E−01 52.46 0.491 1.9E−01 34.00 NUE256 10061 0.384 4.9E−01 13.040.426 4.3E−01 16.39 Control 0.340 0.366 NUE512 9282.3 0.244 2.3E−0228.51 0.370 4.7E−01 3.82 Control 0.190 0.357 NUE514 9403.5 0.245 1.2E−0128.88 0.380 2.4E−01 6.47 NUE514 9402.2 0.225 1.3E−01 18.56 0.373 4.4E−014.60 NUE514 9404.5 0.246 5.1E−02 29.57 0.358 9.5E−01 0.42 NUE514 9402.50.235 4.4E−02 23.86 0.414 3.7E−02 16.10 Control 0.190 0.357 NUE531 100820.393 2.5E−01 15.61 0.445 5.6E−02 21.67 NUE531 10083 0.526 4.9E−02 54.720.503 4.4E−02 37.48 Control 0.340 0.366 NUE532 9222.4 0.238 1.8E−0125.41 0.380 4.4E−01 6.51 NUE532 9223.3 0.217 3.5E−01 13.98 0.418 3.3E−0217.17 NUE532 9223.5 0.261 1.1E−02 37.44 0.404 9.7E−02 13.18 Control0.190 0.357 NUE537 9391.1 0.206 4.1E−01 8.34 0.340 4.9E−01 −4.82 NUE5379393.1 0.204 6.9E−01 7.22 0.369 5.6E−01 3.52 Control 0.190 0.357

Table 52: Analyses of photosynthetic areas (leaf blade area and leafpetiole length) of transgenic plants overexpressing the exogenouspolynucleotides of some embodiments of the invention (using the clonedor synthetic genes listed in Table 23 above) under the regulation of aconstitutive promoter (35S) when grown under standard nitrogen (6 mMKNO₃, 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements) as comparedto control plants. “Ave.”=average.

Example 8 Evaluating Transgenic Plant Growth Under Abiotic StressConditions

One of the consequences of drought is the induction of osmotic stress inthe area surrounding the roots; therefore, in many scientific studies,PEG (e.g., 1.5% PEG8000) is used to simulate the osmotic stressconditions resembling the high osmolarity found during drought stress.

Assay 1: Abiotic Stress Tolerance Assay Under Tissue Culture Conditions—

Plant growth was evaluated under salinity (150 mM NaCl) or osmoticstress [poly (ethylene glycol) (PEG)] in tissue culture conditions.

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (for selecting only transgenic plants). Aftersowing, plates were transferred for 2-3 days for stratification at 4° C.and then grown at 25° C. under 12-hour light 12-hour dark daily cyclesfor 7 to 10 days. At this time point, seedlings randomly chosen werecarefully transferred to plates containing 150 mM or 1.5% PEG: 0.5 MSmedia or Normal growth conditions (0.5 MS media). Each plate contained 5seedlings of the same transgenic event, and 3-4 different plates(replicates) for each event. For each polynucleotide of the invention atleast four independent transformation events were analyzed from eachconstruct. Plants expressing the polynucleotides of the invention werecompared to the average measurement of the control plants (empty vectoror GUS reporter gene under the same promoter) used in the sameexperiment.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincluded 4 light units (4×150 Watts light bulb) and located in adarkroom, was used for capturing images of plantlets sawn in agarplates.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 (Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Seedling Analysis—

Using the digital analysis seedling data was calculated, including leafarea, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following formulas V, VI and VII asdescribed above.Relative growth rate of leaf area=Regression coefficient of leaf areaalong time course.  Formula V:Relative growth rate of root coverage=Regression coefficient of rootcoverage along time course.  Formula VI:Relative growth rate of root length=Regression coefficient of rootcoverage along time course.  Formula VII:

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C., and weighed again to measure plant dryweight for later statistical analysis. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results were used to resolvethe effect of the gene introduced on plant vigor, under osmotic stress,as well as under optimal conditions. Similarly, the effect of the geneintroduced on biomass accumulation, under osmotic stress as well asunder optimal conditions, was determined by comparing the plants' freshand dry weight to that of control plants (containing the GUS reportergene under the same promoter). From every construct created, 3-5independent transformation events were examined in replicates.

Statistical Analyses—

To identify genes conferring significantly improved tolerance to abioticstresses or enlarged root architecture, the results obtained from thetransgenic plants were compared to those obtained from control plants.To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Toevaluate the effect of a gene event over a control the data was analyzedby Student's t-test and the p value was calculated. Results wereconsidered significant if p≦0.1. The JMP statistics software package wasused (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results:

The genes presented in Tables 53, 54 and 55, hereinbelow, have improvedplant ABST (abiotic stress tolerance) when grown under high salinityconcentration levels, compared to control plants. Results showed thatthe genes also improved plant performance under non-salinity conditions.

Tables 53, 54 and 55 depict analyses of plant performance (leaves androots area) under normal (0 mM NaCl) or high salinity (150 mM NaCl)conditions in plants overexpressing the polynucleotides of someembodiments of the invention under the regulation of a constitutivepromoter (35S). Evaluation of each gene was performed by testing theperformance of several events. Some of the genes were evaluated in morethan one tissue culture assay and the results obtained were repeated.Event with p-value<0.1 was considered statistically significant.

TABLE 53 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant performance(leaves and roots area) under normal (standard) conditions Leaves Area[cm²] Roots Area [cm²] Gene % % Treatment name Event Average Statisticsincrement Average Statistics increment 0 mM NaCl CT81 4995.1 0.60 A23.04 0.25 A 57.13 0 mM NaCl CT81 4991.1 0.53 AB 7.91 0.16 B 0.18 0 mMNaCl Control 4543.3 0.49 B 0.16 B

Table 53: Analyses of plant performance (leaves area and root area) oftransgenic plants overexpressing the exogenous polynucleotides of someembodiments of the invention (using the cloned or synthetic genes listedin Table 23 above) under the regulation of a constitutive promoter (35S)when grown under standard conditions (0 mM NaCl) compared to controlplants.

TABLE 54 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant performance(leaves area) under salinity stress Leaves Area [cm²] Treatment Genename Event Average Statistics % increment 150 mM NaCl CT81 4991.1 0.25 A27.57 150 mM NaCl CT81 4995.1 0.21 B 3.74 150 mM NaCl CT81 4993.1 0.20 B2.09 150 mM NaCl Control 4543.3 0.20 B

Table 54: Analyses of plant performance (leaves area) of transgenicplants overexpressing the exogenous polynucleotides of some embodimentsof the invention (using the cloned or synthetic genes listed in Table 23above) under the regulation of a constitutive promoter (35S) when grownunder salinity conditions (150 mM NaCl) compared to control plants.

TABLE 55 Transgenic plants exogenously expressing the polynucleotides ofsome embodiments of the invention exhibit improved plant performance(roots area) under salinity conditions Roots Area [cm²] Treatment Genename Event Average Statistics % increment 150 mM NaCl CT81 4995.1 0.24 A44.30 150 mM NaCl CT81 4991.1 0.22 A 30.09 150 mM NaCl Control 4543.30.17 B 0.00

Table 55: Analyses of plant performance (roots area) of transgenicplants overexpressing the exogenous polynucleotides of some embodimentsof the invention (using the cloned or synthetic genes listed in Table 23above) under the regulation of a constitutive promoter (35S) when grownunder salinity conditions (150 mM NaCl) compared to control plants.

Although the invention has been described in conjunction with specificembodiments thereof, it is evident that many alternatives, modificationsand variations will be apparent to those skilled in the art.Accordingly, it is intended to embrace all such alternatives,modifications and variations that fall within the spirit and broad scopeof the appended claims.

All publications, patents and patent applications mentioned in thisspecification are herein incorporated in their entirety by referenceinto the specification, to the same extent as if each individualpublication, patent or patent application was specifically andindividually indicated to be incorporated herein by reference. Inaddition, citation or identification of any reference in thisapplication shall not be construed as an admission that such referenceis available as prior art to the present invention. To the extent thatsection headings are used, they should not be construed as necessarilylimiting.

What is claimed is:
 1. A method of increasing nitrogen use efficiency,leaf area, growth rate of leaf area, leaf number, leaf blade area,rosette diameter, rosette area, photosynthetic capacity, and/ortolerance to nitrogen deficiency of a plant under nitrogen deficiency,comprising: (a) over-expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence encoding a polypeptidecomprising an amino acid sequence as set forth by SEQ ID NO: 196, (b)selecting a plant transformed with said exogenous polynucleotide for anincreased leaf area, growth rate of leaf area, leaf number, leaf bladearea, rosette diameter, rosette area, nitrogen use efficiency, anincreased photosynthetic capacity, and/or an increased tolerance tonitrogen deficiency under nitrogen deficiency as compared to a nativeplant of the same species, which is grown under the same growthconditions, (c) isolating regenerable portion from plants selectedaccording to step (b), and; (d) regenerating plants from saidregenerable portion isolated according to step (c), thereby increasingthe nitrogen use efficiency, leaf area, growth rate of leaf area, leafnumber, leaf blade area, rosette diameter, rosette area, photosyntheticcapacity, and/or tolerance to nitrogen deficiency of the plant under thenitrogen deficiency.
 2. A method of growing a crop comprising growing acrop plant transformed with an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide comprising an amino acidsequence as set forth by SEQ ID NO: 196, wherein said crop plant isderived from parent plants that have been transformed to express saidexogenous polynucleotide and that have been selected for at least onetrait under nitrogen deficiency selected from the group consisting of:an increased nitrogen use efficiency, an increased leaf area, anincreased growth rate of leaf area, an increased leaf number, anincreased leaf blade area, an increased rosette diameter, an increasedrosette area, an increased photosynthetic capacity and an increasedtolerance to nitrogen deficiency as compared to a native plant of thesame species, which is grown under the same growth conditions, whereinsaid crop plant which comprises said exogenous polynucleotide havingsaid increased nitrogen use efficiency, said increased leaf area, saidincreased growth rate of leaf area, said increased leaf number, saidincreased leaf blade area, said increased rosette diameter, saidincreased rosette area said increased photosynthetic capacity and/orsaid increased tolerance to nitrogen deficiency, thereby growing thecrop.
 3. The method of claim 1, wherein said exogenous polynucleotideencoding said polypeptide is selected from the group consisting of SEQID NOs: 2454, and
 59. 4. The method of claim 1, wherein said nucleicacid is sequence set forth by SEQ ID NO:
 2454. 5. The method of claim 1,further comprising growing the plant expressing said exogenouspolynucleotide under the nitrogen deficiency.
 6. The method of claim 2,wherein said exogenous polynucleotide encoding said polypeptide isselected from the group consisting of SEQ ID NOs: 2454, and
 59. 7. Themethod of claim 2, wherein said nucleic acid is sequence set forth bySEQ ID NO:
 2454. 8. A method of selecting a plant having increasednitrogen use efficiency, leaf area, growth rate of leaf area, leafnumber, leaf blade area, rosette diameter, rosette area, photosyntheticcapacity, and/or tolerance to nitrogen deficiency under nitrogendeficiency as compared to a native plant of the same species, which isgrown under the same growth conditions, the method comprising: (a)providing plants transformed with an exogenous polynucleotide encoding apolypeptide comprising an amino acid sequence set forth by SEQ ID NO:196, (b) selecting said plants of step (a) for increased nitrogen useefficiency, leaf area, growth rate of leaf area, leaf number, leaf bladearea, rosette diameter, rosette area, photosynthetic capacity, and/ortolerance to nitrogen deficiency under nitrogen deficiency as comparedto a native plant of the same species, which is grown under the samegrowth condition, and (c) growing a crop of said plant selected in step(b), thereby selecting the plant having the increased nitrogen useefficiency, leaf area, growth rate of leaf area, leaf number, leaf bladearea, rosette diameter, rosette area, photosynthetic capacity, and/ortolerance to nitrogen deficiency under nitrogen deficiency as comparedto the native plant of the same species, which is grown under the samegrowth conditions.
 9. The method of claim 8, wherein said exogenouspolynucleotide encoding said polypeptide is selected from the groupconsisting of SEQ ID NOs: 2454, and
 59. 10. The method of claim 8,wherein said nucleic acid is sequence set forth by SEQ ID NO:
 2454. 11.A method of increasing nitrogen use efficiency and/or tolerance tonitrogen deficiency of a plant, comprising: (a) over-expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide comprising an amino acid sequence as set forth bySEQ ID NO: 196, and; (b) selecting a plant transformed with saidexogenous polynucleotide for an increased nitrogen use efficiency,and/or an increased tolerance to nitrogen deficiency as compared to anative plant of the same species, which is grown under the same growthconditions, thereby increasing the nitrogen use efficiency, and/or thetolerance to nitrogen deficiency of the plant.
 12. The method of claim11, wherein said exogenous polynucleotide encoding said polypeptide isselected from the group consisting of SEQ ID NOs: 2454, and
 59. 13. Themethod of claim 11, wherein said nucleic acid is sequence set forth bySEQ ID NO:
 2454. 14. The method of claim 11, further comprising growingthe plant expressing said exogenous polynucleotide under nitrogendeficiency.
 15. A method of growing a crop comprising growing a cropplant transformed with an exogenous polynucleotide comprising a nucleicacid sequence encoding a polypeptide comprising an amino acid sequenceas set forth by SEQ ID NO: 196, wherein said crop plant is derived fromparent plants that have been transformed to express said exogenouspolynucleotide and that have been selected for at least one traitselected from the group consisting of: an increased nitrogen useefficiency and an increased tolerance to nitrogen deficiency as comparedto a native plant of the same species, which is grown under the samegrowth conditions, wherein said crop plant which comprises saidexogenous polynucleotide having said increased nitrogen use efficiency,and/or said increased tolerance to nitrogen deficiency, thereby growingthe crop.
 16. The method of claim 15, wherein said exogenouspolynucleotide encoding said polypeptide is selected from the groupconsisting of SEQ ID NOs: 2454, and
 59. 17. The method of claim 15,wherein said nucleic acid is sequence set forth by SEQ ID NO:
 2454. 18.A method of selecting a plant having increased nitrogen use efficiencyand/or tolerance to nitrogen deficiency as compared to a native plant ofthe same species, which is grown under the same growth conditions, themethod comprising: (a) providing plants transformed with an exogenouspolynucleotide encoding a polypeptide comprising an amino acid sequenceas set forth by SEQ ID NO: 196, (b) selecting said plants of step (a)for increased nitrogen use efficiency and/or tolerance to nitrogendeficiency as compared to a native plant of the same species, which isgrown under the same growth condition, and (c) growing a crop of saidplant selected in step (b), thereby selecting the plant having theincreased nitrogen use efficiency and/or tolerance to nitrogendeficiency as compared to the native plant of the same species, which isgrown under the same growth conditions.
 19. The method of claim 18,wherein said exogenous polynucleotide encoding said polypeptide isselected from the group consisting of SEQ ID NOs: 2454, and
 59. 20. Themethod of claim 18, wherein said nucleic acid is sequence set forth bySEQ ID NO: 2454.